Chain F, Twinkle mtDNA helicase
bifunctional DNA primase/helicase( domain architecture ID 10099140)
bifunctional DNA primase/helicase similar to mitochondrial Twinkle protein, chloroplastic Twinkle homolog, and bacteriophage T7 gp4, which have both DNA primase and helicase activities
List of domain hits
Name | Accession | Description | Interval | E-value | |||||
Twinkle_C | cd01122 | C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ... |
246-509 | 4.50e-179 | |||||
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases. : Pssm-ID: 410867 Cd Length: 266 Bit Score: 504.46 E-value: 4.50e-179
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TOPRIM_primases | cd01029 | TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain ... |
139-215 | 2.14e-03 | |||||
TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain found in the active site regions of bacterial DnaG-type primases and their homologs. Primases synthesize RNA primers for the initiation of DNA replication. DnaG type primases are often closely associated with DNA helicases in primosome assemblies. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). This glutamate and two aspartates, cluster together to form a highly acid surface patch. The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function. The prototypical bacterial primase. Escherichia coli DnaG is a single subunit enzyme. : Pssm-ID: 173779 [Multi-domain] Cd Length: 79 Bit Score: 37.25 E-value: 2.14e-03
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Name | Accession | Description | Interval | E-value | |||||
Twinkle_C | cd01122 | C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ... |
246-509 | 4.50e-179 | |||||
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases. Pssm-ID: 410867 Cd Length: 266 Bit Score: 504.46 E-value: 4.50e-179
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AAA_25 | pfam13481 | AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins. |
273-444 | 2.03e-23 | |||||
AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins. Pssm-ID: 463892 [Multi-domain] Cd Length: 193 Bit Score: 97.84 E-value: 2.03e-23
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RepA | COG3598 | RecA-family ATPase [Replication, recombination and repair]; |
281-490 | 3.11e-11 | |||||
RecA-family ATPase [Replication, recombination and repair]; Pssm-ID: 442817 [Multi-domain] Cd Length: 313 Bit Score: 64.54 E-value: 3.11e-11
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PRK08506 | PRK08506 | replicative DNA helicase; Provisional |
231-406 | 2.49e-05 | |||||
replicative DNA helicase; Provisional Pssm-ID: 236278 [Multi-domain] Cd Length: 472 Bit Score: 46.93 E-value: 2.49e-05
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TOPRIM_primases | cd01029 | TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain ... |
139-215 | 2.14e-03 | |||||
TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain found in the active site regions of bacterial DnaG-type primases and their homologs. Primases synthesize RNA primers for the initiation of DNA replication. DnaG type primases are often closely associated with DNA helicases in primosome assemblies. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). This glutamate and two aspartates, cluster together to form a highly acid surface patch. The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function. The prototypical bacterial primase. Escherichia coli DnaG is a single subunit enzyme. Pssm-ID: 173779 [Multi-domain] Cd Length: 79 Bit Score: 37.25 E-value: 2.14e-03
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ATPase_ComGA | NF041000 | competence type IV pilus ATPase ComGA; |
275-303 | 8.16e-03 | |||||
competence type IV pilus ATPase ComGA; Pssm-ID: 468930 [Multi-domain] Cd Length: 265 Bit Score: 38.20 E-value: 8.16e-03
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Name | Accession | Description | Interval | E-value | |||||
Twinkle_C | cd01122 | C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid ... |
246-509 | 4.50e-179 | |||||
C-terminal domain of Twinkle; Twinkle ( T7 gp4-like protein with intramitochondrial nucleoid localization, also known as C10orf2, PEO1, SCA8, ATXN8, IOSCA, PEOA3 or SANDO) is a homohexameric DNA helicases which unwinds short stretches of double-stranded DNA in the 5' to 3' direction and, along with mitochondrial single-stranded DNA binding protein and mtDNA polymerase gamma, is thought to play a key role in mtDNA replication. Mutations in the human gene cause infantile onset spinocerebellar ataxia (IOSCA) and progressive external ophthalmoplegia (PEO) and are also associated with several mitochondrial depletion syndromes. This group also contains viral GP4-like and related bacterial helicases. Pssm-ID: 410867 Cd Length: 266 Bit Score: 504.46 E-value: 4.50e-179
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AAA_25 | pfam13481 | AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins. |
273-444 | 2.03e-23 | |||||
AAA domain; This AAA domain is found in a wide variety of presumed DNA repair proteins. Pssm-ID: 463892 [Multi-domain] Cd Length: 193 Bit Score: 97.84 E-value: 2.03e-23
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RecA-like_Gp4D_helicase | cd19483 | RecA-like domain of Escherichia coli bacteriophage T7 Gp4D helicase; This family includes the ... |
291-499 | 4.73e-16 | |||||
RecA-like domain of Escherichia coli bacteriophage T7 Gp4D helicase; This family includes the RecA-like domain of the Gp4D fragment of the Gene4 helicase-primase (Gp4) from bacteriophage T7. Gp4D (residues 241-566) is the minimal fragment of the Gp4 that forms hexameric rings, it contains the helicase domain and the linker connecting the helicase and primase domains. Helicases are ring-shaped oligomeric enzymes that unwind DNA at the replication fork; they couple NTP hydrolysis to the unwinding of nucleic acid duplexes into their component strands. This family belongs to the RecA-like NTPase superfamily which includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. The RecA-like NTPase family also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion. Pssm-ID: 410891 [Multi-domain] Cd Length: 231 Bit Score: 77.61 E-value: 4.73e-16
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RepA | COG3598 | RecA-family ATPase [Replication, recombination and repair]; |
281-490 | 3.11e-11 | |||||
RecA-family ATPase [Replication, recombination and repair]; Pssm-ID: 442817 [Multi-domain] Cd Length: 313 Bit Score: 64.54 E-value: 3.11e-11
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RecA-like_superfamily | cd01120 | RecA-like_NTPases; RecA-like NTPases. This superfamily includes the NTP binding domain of F1 ... |
291-456 | 8.34e-11 | |||||
RecA-like_NTPases; RecA-like NTPases. This superfamily includes the NTP binding domain of F1 and V1 H(+)ATPases, DnaB and related helicases as well as bacterial RecA and related eukaryotic and archaeal recombinases. This group also includes bacterial conjugation proteins and related DNA transfer proteins involved in type II and type IV secretion. Pssm-ID: 410865 [Multi-domain] Cd Length: 119 Bit Score: 59.44 E-value: 8.34e-11
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RAD55 | COG0467 | RecA-superfamily ATPase, KaiC/GvpD/RAD55 family [Signal transduction mechanisms]; |
273-489 | 2.06e-05 | |||||
RecA-superfamily ATPase, KaiC/GvpD/RAD55 family [Signal transduction mechanisms]; Pssm-ID: 440235 [Multi-domain] Cd Length: 221 Bit Score: 45.68 E-value: 2.06e-05
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PRK08506 | PRK08506 | replicative DNA helicase; Provisional |
231-406 | 2.49e-05 | |||||
replicative DNA helicase; Provisional Pssm-ID: 236278 [Multi-domain] Cd Length: 472 Bit Score: 46.93 E-value: 2.49e-05
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DnaB_C | cd00984 | C-terminal domain of DnaB helicase; DnaB helicase C-terminal domain. The hexameric helicase ... |
273-401 | 6.28e-05 | |||||
C-terminal domain of DnaB helicase; DnaB helicase C-terminal domain. The hexameric helicase DnaB unwinds the DNA duplex at the chromosome replication fork. Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerization of the N-terminal domain has been observed and may occur during the enzymatic cycle. This C-terminal domain contains an ATP-binding site and is therefore probably the site of ATP hydrolysis. Pssm-ID: 410864 [Multi-domain] Cd Length: 256 Bit Score: 44.81 E-value: 6.28e-05
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TOPRIM_primases | cd01029 | TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain ... |
139-215 | 2.14e-03 | |||||
TOPRIM_primases: The topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain found in the active site regions of bacterial DnaG-type primases and their homologs. Primases synthesize RNA primers for the initiation of DNA replication. DnaG type primases are often closely associated with DNA helicases in primosome assemblies. The TOPRIM domain has two conserved motifs, one of which centers at a conserved glutamate and the other one at two conserved aspartates (DxD). This glutamate and two aspartates, cluster together to form a highly acid surface patch. The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function. The prototypical bacterial primase. Escherichia coli DnaG is a single subunit enzyme. Pssm-ID: 173779 [Multi-domain] Cd Length: 79 Bit Score: 37.25 E-value: 2.14e-03
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ABC_ATPase | cd00267 | ATP-binding cassette transporter nucleotide-binding domain; ABC transporters are a large ... |
286-338 | 3.83e-03 | |||||
ATP-binding cassette transporter nucleotide-binding domain; ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules. The nucleotide-binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins. Pssm-ID: 213179 [Multi-domain] Cd Length: 157 Bit Score: 38.38 E-value: 3.83e-03
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AAA_16 | pfam13191 | AAA ATPase domain; This family of domains contain a P-loop motif that is characteriztic of the ... |
277-399 | 3.83e-03 | |||||
AAA ATPase domain; This family of domains contain a P-loop motif that is characteriztic of the AAA superfamily. Pssm-ID: 433025 [Multi-domain] Cd Length: 167 Bit Score: 38.25 E-value: 3.83e-03
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ABC_Class3 | cd03229 | ATP-binding cassette domain of the binding protein-dependent transport systems; This class is ... |
286-333 | 6.26e-03 | |||||
ATP-binding cassette domain of the binding protein-dependent transport systems; This class is comprised of all BPD (Binding Protein Dependent) systems that are largely represented in archaea and eubacteria and are primarily involved in scavenging solutes from the environment. ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules. The nucleotide binding domain shows the highest similarity between all members of the family. ABC transporters are a subset of nucleotide hydrolases that contain a signature motif, Q-loop, and H-loop/switch region, in addition to, the Walker A motif/P-loop and Walker B motif commonly found in a number of ATP- and GTP-binding and hydrolyzing proteins. Pssm-ID: 213196 [Multi-domain] Cd Length: 178 Bit Score: 37.94 E-value: 6.26e-03
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ABCC_MRP_domain1 | cd03250 | ATP-binding cassette domain 1 of multidrug resistance-associated protein, subfamily C; This ... |
286-306 | 7.61e-03 | |||||
ATP-binding cassette domain 1 of multidrug resistance-associated protein, subfamily C; This subfamily is also known as MRP (multidrug resistance-associated protein). Some of the MRP members have five additional transmembrane segments in their N-terminus, but the function of these additional membrane-spanning domains is not clear. The MRP was found in the multidrug-resisting lung cancer cell in which p-glycoprotein was not overexpressed. MRP exports glutathione by drug stimulation, as well as, certain substrates in conjugated forms with anions, such as glutathione, glucuronate, and sulfate. Pssm-ID: 213217 [Multi-domain] Cd Length: 204 Bit Score: 37.83 E-value: 7.61e-03
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ATPase_ComGA | NF041000 | competence type IV pilus ATPase ComGA; |
275-303 | 8.16e-03 | |||||
competence type IV pilus ATPase ComGA; Pssm-ID: 468930 [Multi-domain] Cd Length: 265 Bit Score: 38.20 E-value: 8.16e-03
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DnaB | COG0305 | Replicative DNA helicase [Replication, recombination and repair]; |
245-313 | 8.20e-03 | |||||
Replicative DNA helicase [Replication, recombination and repair]; Pssm-ID: 440074 [Multi-domain] Cd Length: 429 Bit Score: 38.90 E-value: 8.20e-03
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Blast search parameters | ||||
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