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Conserved domains on  [gi|166230036|sp|A1UBJ0|]
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RecName: Full=Large ribosomal subunit protein uL10; AltName: Full=50S ribosomal protein L10

Protein Classification

50S ribosomal protein L10( domain architecture ID 10785131)

50S ribosomal protein L10 binds and anchors the two L7/L12 dimers to the large ribosomal subunit

Gene Ontology:  GO:0006412|GO:0015934|GO:0070180
PubMed:  15989950|28524878

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RplJ COG0244
Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 ...
 1-171 7.83e-62

Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 is part of the Pathway/BioSystem: Ribosome 50S subunit


:

Pssm-ID: 440014  Cd Length: 172  Bit Score: 188.74  E-value: 7.83e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:COG0244    1 MTREEKKAVVAELKEKLKEAQAVVLTDYRGLTVAQMTELRRKLREAgVEYKVVKNTLARIALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036  80 AFVQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQVA 159
Cdd:COG0244   81 AFSYEDPVAAAKVLKDFAKENEKLVIKGGVLDGKVLDAEQVKALAKLPSREELLAKLLGLLQAPATKLVGVLNAPPRKLA 160

                        170
                 ....*....|..
gi 166230036 160 RLAAALQEKKAG 171
Cdd:COG0244  161 RVLKAIAEKKEA 172
 
Name Accession Description Interval E-value
RplJ COG0244
Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 ...
 1-171 7.83e-62

Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 440014  Cd Length: 172  Bit Score: 188.74  E-value: 7.83e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:COG0244    1 MTREEKKAVVAELKEKLKEAQAVVLTDYRGLTVAQMTELRRKLREAgVEYKVVKNTLARIALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036  80 AFVQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQVA 159
Cdd:COG0244   81 AFSYEDPVAAAKVLKDFAKENEKLVIKGGVLDGKVLDAEQVKALAKLPSREELLAKLLGLLQAPATKLVGVLNAPPRKLA 160

                        170
                 ....*....|..
gi 166230036 160 RLAAALQEKKAG 171
Cdd:COG0244  161 RVLKAIAEKKEA 172
rplJ PRK00099
50S ribosomal protein L10; Reviewed
 1-171 8.62e-60

50S ribosomal protein L10; Reviewed


Pssm-ID: 234632  Cd Length: 172  Bit Score: 183.42  E-value: 8.62e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:PRK00099   1 MNREEKKEIVAELAEKLKKAQSAVVADYRGLTVAQMTELRKKLREAgVEYKVVKNTLARRALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036  80 AFVQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQVA 159
Cdd:PRK00099  81 AFSYEDPVAAAKVLKDFAKDNKKLEIKGGAIEGKVLDAEEVKALAKLPSREELLAKLLGVLQAPATKLAGVLNAPPSKLA 160

                        170
                 ....*....|..
gi 166230036 160 RLAAALQEKKAG 171
Cdd:PRK00099 161 RVLKALAEKKEA 172
Ribosomal_L10 cd05797
Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and ...
 3-157 4.91e-49

Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and eukaryotic mitochondrial 39S ribosomal protein, L10. L10 occupies the L7/L12 stalk of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These bacteria and eukaryotic sequences have no additional C-terminal domain, present in other eukaryotic and archaeal orthologs.


Pssm-ID: 240223  Cd Length: 157  Bit Score: 155.74  E-value: 4.91e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   3 KADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAIAF 81
Cdd:cd05797    2 REKKEEIVAELKEKLKEAKSVVVADYRGLTVAQLTELRKELREAgVKLKVVKNTLAKRALEGTGFEDLDDLLKGPTAIAF 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 166230036  82 VQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQ 157
Cdd:cd05797   82 SEEDPVAAAKVLKDFAKENKKLEIKGGVVEGKVLDAEEVKALAKLPSREELLAQLLGLLQAPATKLVRVLNAPASK 157
Ribosomal_L10 pfam00466
Ribosomal protein L10;
1-88 1.03e-24

Ribosomal protein L10;


Pssm-ID: 459822 [Multi-domain]  Cd Length: 99  Bit Score: 91.84  E-value: 1.03e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036    1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:pfam00466   1 KTREKKEELVEELKELLKEYKSVVVVDYRGLTVAQLTELRKKLRENgAELKVGKNTLMRRALEETGEEKLEDYLKGPTAL 80


                  ....*....
gi 166230036   80 AFVQGEPVD 88
Cdd:pfam00466  81 LFTNEDPVA 89
 
Name Accession Description Interval E-value
RplJ COG0244
Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 ...
 1-171 7.83e-62

Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 440014  Cd Length: 172  Bit Score: 188.74  E-value: 7.83e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:COG0244    1 MTREEKKAVVAELKEKLKEAQAVVLTDYRGLTVAQMTELRRKLREAgVEYKVVKNTLARIALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036  80 AFVQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQVA 159
Cdd:COG0244   81 AFSYEDPVAAAKVLKDFAKENEKLVIKGGVLDGKVLDAEQVKALAKLPSREELLAKLLGLLQAPATKLVGVLNAPPRKLA 160

                        170
                 ....*....|..
gi 166230036 160 RLAAALQEKKAG 171
Cdd:COG0244  161 RVLKAIAEKKEA 172
rplJ PRK00099
50S ribosomal protein L10; Reviewed
 1-171 8.62e-60

50S ribosomal protein L10; Reviewed


Pssm-ID: 234632  Cd Length: 172  Bit Score: 183.42  E-value: 8.62e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:PRK00099   1 MNREEKKEIVAELAEKLKKAQSAVVADYRGLTVAQMTELRKKLREAgVEYKVVKNTLARRALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036  80 AFVQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQVA 159
Cdd:PRK00099  81 AFSYEDPVAAAKVLKDFAKDNKKLEIKGGAIEGKVLDAEEVKALAKLPSREELLAKLLGVLQAPATKLAGVLNAPPSKLA 160

                        170
                 ....*....|..
gi 166230036 160 RLAAALQEKKAG 171
Cdd:PRK00099 161 RVLKALAEKKEA 172
Ribosomal_L10 cd05797
Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and ...
 3-157 4.91e-49

Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and eukaryotic mitochondrial 39S ribosomal protein, L10. L10 occupies the L7/L12 stalk of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These bacteria and eukaryotic sequences have no additional C-terminal domain, present in other eukaryotic and archaeal orthologs.


Pssm-ID: 240223  Cd Length: 157  Bit Score: 155.74  E-value: 4.91e-49
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   3 KADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAIAF 81
Cdd:cd05797    2 REKKEEIVAELKEKLKEAKSVVVADYRGLTVAQLTELRKELREAgVKLKVVKNTLAKRALEGTGFEDLDDLLKGPTAIAF 81

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 166230036  82 VQGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQ 157
Cdd:cd05797   82 SEEDPVAAAKVLKDFAKENKKLEIKGGVVEGKVLDAEEVKALAKLPSREELLAQLLGLLQAPATKLVRVLNAPASK 157
Ribosomal_L10_P0 cd00379
Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in ...
 4-157 2.91e-41

Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in bacteria, P0 in eukaryotes, and L10e in archaea, as well as uncharacterized P0-like eukaryotic proteins. In all three kingdoms, L10 forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). Some eukaryotic P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 238222 [Multi-domain]  Cd Length: 155  Bit Score: 135.77  E-value: 2.91e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   4 ADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAIAFV 82
Cdd:cd00379    1 EKKEELVEELKELLKKYKSVVVVDYRGLTVAQLTELRKELRESgAKLKVGKNTLMRRALKGTGFEELKPLLKGPTALAFT 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 166230036  83 QGEPVDAAKAIKAFAKEHKALVIKGGYMEGRALSIDEVNRIADLESREVLLAKLAGAMKGNLAKAAGLFNAPASQ 157
Cdd:cd00379   81 NEDPVEVAKVLKDFAKENKKLFAKGGVVAGKVLDPAGVTALAKLPSREELLAMLIGLLKAPIAKLARLLNALGIG 155
Ribosomal_L10 pfam00466
Ribosomal protein L10;
1-88 1.03e-24

Ribosomal protein L10;


Pssm-ID: 459822 [Multi-domain]  Cd Length: 99  Bit Score: 91.84  E-value: 1.03e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036    1 MAKADKATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGES-ATYTVAKNTLVKRAAAEAGIDGLDELFTGPTAI 79
Cdd:pfam00466   1 KTREKKEELVEELKELLKEYKSVVVVDYRGLTVAQLTELRKKLRENgAELKVGKNTLMRRALEETGEEKLEDYLKGPTAL 80


                  ....*....
gi 166230036   80 AFVQGEPVD 88
Cdd:pfam00466  81 LFTNEDPVA 89
rplP0 PRK04019
acidic ribosomal protein P0; Validated
 6-86 2.14e-05

acidic ribosomal protein P0; Validated


Pssm-ID: 179712 [Multi-domain]  Cd Length: 330  Bit Score: 43.32  E-value: 2.14e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 166230036   6 KATAVADIAEQFKEATATVVTEYRGLTVANLAQLRRSLGESATYTVAKNTLVKRAAAEAGIDGLDEL---FTGPTAIAFV 82
Cdd:PRK04019   8 KKEEVEELKELIKSYPVVGIVDLEGIPARQLQEIRRKLRGKAELKVSKNTLIKRALEEAGEEDLEKLedyLEGQVALIFT 87


                 ....
gi 166230036  83 QGEP 86
Cdd:PRK04019  88 NMNP 91
Ribosomal_P0_L10e cd05795
Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal ...
 36-86 3.38e-03

Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal protein P0 and the archaeal P0 homolog, L10e. P0 or L10e forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These eukaryotic and archaeal P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 240221 [Multi-domain]  Cd Length: 175  Bit Score: 36.40  E-value: 3.38e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 166230036  36 LAQLRRSLGESATYTVAKNTLVKRAAAEAG-----IDGLDELFTGPTAIAFVQGEP 86
Cdd:cd05795   33 LQKIRRSLRGKAEILMGKNTLIRRALRNLGdenpeLEKLLPYLKGNVGFIFTNGDP 88
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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