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Conserved domains on  [gi|85680313|gb|ABC72343|]
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pyrroloquinoline quinone biosynthesis protein [uncultured haloarchaeon]

Protein Classification

TenA family protein( domain architecture ID 10002780)

TenA family protein such as Bacillus halodurans aminopyrimidine aminohydrolase, which catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, as part of the thiamine salvage pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 6-217 1.60e-85

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


:

Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 252.50  E-value: 1.60e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:COG0819   4 SERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVEL 83

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE-LTDGTHEYTPFIDT 164
Cdd:COG0819  84 ALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAErPLPPDHPYAEWIET 163

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 85680313 165 YTSEEFRGDVAWGRSFVDDCWERY-PGEREAMEEAFIMSAKLEYRFWEMAYTTE 217
Cdd:COG0819 164 YASEEFQELVEWLIALLDRLAATAsEAERERLEEAFRTASRLEYAFWDMAYRLE 217
 
Name Accession Description Interval E-value
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 6-217 1.60e-85

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 252.50  E-value: 1.60e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:COG0819   4 SERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVEL 83

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE-LTDGTHEYTPFIDT 164
Cdd:COG0819  84 ALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAErPLPPDHPYAEWIET 163

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 85680313 165 YTSEEFRGDVAWGRSFVDDCWERY-PGEREAMEEAFIMSAKLEYRFWEMAYTTE 217
Cdd:COG0819 164 YASEEFQELVEWLIALLDRLAATAsEAERERLEEAFRTASRLEYAFWDMAYRLE 217
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 6-214 7.82e-85

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 250.55  E-value: 7.82e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19366   2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEM 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE--LTDGTHEYTPFID 163
Cdd:cd19366  82 DLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEqgGALEHNPYREWIE 161

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|..
gi 85680313 164 TYTSEEFRGDVAWGRSFVDDCWERYPG-EREAMEEAFIMSAKLEYRFWEMAY 214
Cdd:cd19366 162 MYSSDEFTELADWLIDLLDELAEGKSEaELERLEEIFLTSSRYEYMFWDMAY 213
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 14-215 1.32e-49

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 160.82  E-value: 1.32e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    14 ESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFAA 93
Cdd:TIGR04306   2 KPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    94 DYGISQEELEATAK-APTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAEL-TDGTHE-YTPFIDTYTSEEF 170
Cdd:TIGR04306  82 VLGISDEEYFQKIKpGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKhTAPEHSvYHKWIETYSSSWF 161

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 85680313   171 RGDVAWGRSFVDDCWERYPGE-REAMEEAFIMSAKLEYRFWEMAYT 215
Cdd:TIGR04306 162 REAVNEGENLLNHLAETSSPEeLEKLKTIFAESCEYEYNFWTMAYE 207
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-215 6.51e-45

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 148.66  E-value: 6.51e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    14 ESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFAA 93
Cdd:pfam03070   5 RPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    94 DYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE-LTDGTH-EYTPFIDTYTSEEFR 171
Cdd:pfam03070  85 ALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEkIRALEGpEYYEWVKTYASEYFR 164

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 85680313   172 GDVAWGRSFVDDCWERYPGER-EAMEEAFIMSAKLEYRFWEMAYT 215
Cdd:pfam03070 165 AAVEEAKRLLDRLLAYVSDEQlEELQEIFLKALEFEYSFWTMALD 209
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
6-213 2.55e-35

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 130.68  E-value: 2.55e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:PRK14713 321 TAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVES 400

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   86 DLHRSFAADYGISqeeleaTAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIAdhmAELTD-----GTHEYTP 160
Cdd:PRK14713 401 ELHRSWLGDRDAD------TAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVG---AELHAragnpDDHPYAE 471

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 85680313  161 FIDTYTSEEFRGDVAWGRSFVDDCWER-YPGEREAMEEAFIMSAKLEYRFWEMA 213
Cdd:PRK14713 472 WLQTYADPEFAAATRRAIAFVDRAFRAaSPAERAAMARAFLTACRYELEFFDQA 525
 
Name Accession Description Interval E-value
TenA COG0819
Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and ...
 6-217 1.60e-85

Aminopyrimidine aminohydrolase TenA (thiamine salvage pathway) [Coenzyme transport and metabolism];


Pssm-ID: 440581 [Multi-domain]  Cd Length: 218  Bit Score: 252.50  E-value: 1.60e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:COG0819   4 SERLREAAAPIWEAILEHPFVQGLADGTLPREAFRHYLVQDYLYLEHFARALALAAAKAPDLEDMRFLAGLAAGLLEVEL 83

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE-LTDGTHEYTPFIDT 164
Cdd:COG0819  84 ALHERYAAELGISEEELEATPPSPTTRAYTSYLLAAAASGDYAELLAALLPCEWGYAEIGKRLAErPLPPDHPYAEWIET 163

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 85680313 165 YTSEEFRGDVAWGRSFVDDCWERY-PGEREAMEEAFIMSAKLEYRFWEMAYTTE 217
Cdd:COG0819 164 YASEEFQELVEWLIALLDRLAATAsEAERERLEEAFRTASRLEYAFWDMAYRLE 217
TenA_C_BhTenA-like cd19366
TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to ...
 6-214 7.82e-85

TenA_C proteins similar to Bacillus halodurans TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus halodurans TenA which participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381701 [Multi-domain]  Cd Length: 213  Bit Score: 250.55  E-value: 7.82e-85
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19366   2 SERLREKAKPIWEAGLEHPFVQGLGDGTLDKEKFKFYLKQDYLYLIDYARVFALGAAKADDLETMGRFAELLHGTLNTEM 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE--LTDGTHEYTPFID 163
Cdd:cd19366  82 DLHRQYAAEFGITEEELEATEPSPTTLAYTSYMLRTAQTGTLAELLAALLPCAWGYAEIGKRLAEqgGALEHNPYREWIE 161

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|..
gi 85680313 164 TYTSEEFRGDVAWGRSFVDDCWERYPG-EREAMEEAFIMSAKLEYRFWEMAY 214
Cdd:cd19366 162 MYSSDEFTELADWLIDLLDELAEGKSEaELERLEEIFLTSSRYEYMFWDMAY 213
TenA_PqqC-like cd16099
TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone ...
 16-212 3.43e-67

TenA-like proteins including TenA_C and TenA_E proteins, as well as pyrroloquinoline quinone (PQQ) synthesis protein C; TenA proteins participate in thiamin metabolism and can be classified into two classes: TenA_C which has an active site Cys, and TenA_E which does not; TenA_E proteins often have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product amino-HMP (4-amino-5-amino-methyl-2-methylpyrimidine) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin. Bacillus subtilis TenA_C can hydrolyze amino-HMP to AMP and can catalyze the hydrolysis of thiamin. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal ThiD domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). Bacillus halodurans TenA_C participates in a salvage pathway where the thiamine degradation product 2-methyl-4-formylamino-5-aminomethylpyrimidine (formylamino-HMP) is hydrolyzed first to amino-HMP by the YlmB protein, and the amino-HMP is then hydrolyzed by TenA to produce HMP. Helicobacter pylori TenA_C is also thought to catalyze a salvage reaction but the pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect; Pyrococcus furiosus TenA_E lacks appropriate surface charges for DNA interactions. This family also includes bacterial coenzyme pyrroloquinoline quinone (PQQ) synthesis protein C (PQQC), an oxidase involved in the final step of PQQ biosynthesis, and CADD, a Chlamydia protein that interacts with death receptors.


Pssm-ID: 381691 [Multi-domain]  Cd Length: 196  Bit Score: 205.28  E-value: 3.43e-67
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  16 IWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTaLLDVAHTILETEMDLHRSFAADY 95
Cdd:cd16099   1 IWDAILNHPFVQELAAGTLPREKFRYYLAQDYYYLKDFARALALAAAKAPDLELRT-FLAELINVLDDELELHEKLLAEL 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  96 GISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHEYTPFIDTYTSEEFRGDVA 175
Cdd:cd16099  80 GISEEDLSEAEPNPATLAYTNHLLRVAARGTPAEGLAALLPCYWSYGEIGRRLAASLPEHPPYRFWIDFYASDEYQELVE 159

                       170       180       190
                ....*....|....*....|....*....|....*..
gi 85680313 176 WGRSFVDDCWERYPGEREAMEEAFIMSAKLEYRFWEM 212
Cdd:cd16099 160 ELLQLLDQLAAAGEEEKEELKEIFLTSLRYELMFWDA 196
TenA_C-like cd19365
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 6-213 5.89e-62

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381700 [Multi-domain]  Cd Length: 205  Bit Score: 191.96  E-value: 5.89e-62
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19365   1 TEALWEAIAPIYAAILAHPFIRELADGTLPREKFRFYLAQDALYLRDYARALALLAARAPDPEEQVFFARFAAGAIEVER 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISqeelEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHEYTPFIDTY 165
Cdd:cd19365  81 ELHRSFLGEFGID----AAAEPSPVTLAYTSFLLATAATGPYAVAVAAVLPCFWIYAEVGKRLAAAASPNHPYQDWIDTY 156

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*....
gi 85680313 166 TSEEFRGDVAWGRSFVDDCWER-YPGEREAMEEAFIMSAKLEYRFWEMA 213
Cdd:cd19365 157 SDEEFAEAVRRAIAIVDRLAAEaSPEERARMLEAFLRASRLEWMFWDAA 205
TenA_C_BsTenA-like cd19364
TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the ...
 6-215 1.07e-57

TenA_C proteins similar to Bacillus subtilis TenA; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Bacillus subtilis TenA which has been shown to be a thiaminase II, catalyzing the hydrolysis of thiamine into HMP and 5-(2-hydroxyethyl)-4-methylthiazole (THZ), within thiamine metabolism. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381699 [Multi-domain]  Cd Length: 212  Bit Score: 181.61  E-value: 1.07e-57
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19364   2 TEELREAADPLWQKSFEHPFIQGLADGTLPLETFRYYLIQDAYYLKHFAKLHALAAAKADDPAIKALLLEGAQGLAEGEL 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHEYTPFIDTY 165
Cdd:cd19364  82 ALRETFFKELGITEEEIAQTPPAPTAYHYVSHMYRQLNEGSVAEAVAALLPCYWLYQEIGERLADAGSPVPLYQRWIDTY 161

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|.
gi 85680313 166 TSEEFRGDVAWGRSFVDDCWER-YPGEREAMEEAFIMSAKLEYRFWEMAYT 215
Cdd:cd19364 162 ASDEFTESVQQQIDLVDRLAEEaSEEEREKMKQAFLISSYYELQFWEMAYT 212
TenA_C_ScTHI20-like cd19367
TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 ...
 16-214 2.80e-56

TenA_C family similar to the C-terminal TenA_C domain of Saccharomyces cerevisiae THI20 protein; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Saccharomyces cerevisiae THI20 includes a C-terminal tetrameric TenA-like domain fused to an N-terminal HMP kinase/HMP-P kinase (ThiD-like) domain, and participates in thiamin biosynthesis, degradation and salvage; the TenA-like domain catalyzes the production of HMP from thiamin degradation products (salvage). A majority of this family are single-domain TenA_C- like proteins; some however have additional domains such as a ThiD domain. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381702 [Multi-domain]  Cd Length: 204  Bit Score: 177.70  E-value: 2.80e-56
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  16 IWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILEtEMDLHRSFAADY 95
Cdd:cd19367   2 IWKAYTNHPFVRQLADGTLPLESFRHYLKQDYLYLIHYARAHALAAYKAPTLEDIRAAAAILLAILE-EMELHVKYCAEW 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  96 GISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHE---YTPFIDTYTSEEFRG 172
Cdd:cd19367  81 GISEEELEATPESPATLAYTRYVLDVGLSGDLLDLLVALAPCLLGYGEIGLRLAADPATKLEgnpYWSWIETYASDEYQE 160

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....
gi 85680313 173 DVAWGRSFVDDCWERYPGER--EAMEEAFIMSAKLEYRFWEMAY 214
Cdd:cd19367 161 AVREGIELLEKLAAERPSPArlEELVKIFATATRLEIGFWDMGL 204
TenA_C_HP1287-like cd19361
TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins ...
 6-212 8.72e-56

TenA_C proteins similar to Helicobacter pylori TenA (HP1287; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Helicobacter pylori TenA (HP1287) protein which is thought to catalyze a salvage reaction in thiamin metabolism, however its pyrimidine substrate has not yet been identified. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. HP1287 may contribute to stomach colonization and persistence.


Pssm-ID: 381696 [Multi-domain]  Cd Length: 212  Bit Score: 176.62  E-value: 8.72e-56
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19361   2 SERLYEAVEDIWDSYYEHPFVQGIADGTLDIEKFRFYMIQDYLYLLDYAKVFALGVAKAKDEEVMRFFADLINAILNEEM 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAEL--TDGTHE-YTPFI 162
Cdd:cd19361  82 DIHRGYMKRLGITEEEIENTKPALDNLSYTSYMLSVAYEGGIAEILAAILSCSWSYEYIAKKLVERypAALEHEfYGEWV 161

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|.
gi 85680313 163 DTYTSEEFRGDVAWGRSFVDDCWERYP-GEREAMEEAFIMSAKLEYRFWEM 212
Cdd:cd19361 162 KGYSSEEYAEANQELIDLLDRLTEDISeEQIEKLEEIFVNCSRYELKFWDM 212
salvage_TenA TIGR04306
thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the ...
 14-215 1.32e-49

thiaminase II; The TenA protein of Bacillus subtilis and Staphylococcus aurues, and the C-terminal region of trifunctional protein Thi20p from Saccharomyces cerevisiae, perform cleavages on thiamine and related compounds to produce 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), a substrate a salvage pathway for thiamine biosynthesis. The gene symbol tenA, for Transcription ENhancement A, reflects a misleading early characterization as a regulatory protein. This family is related to PqqC from the PQQ biosynthesis system (see TIGR02111), heme oxygenase (pfam01126), and CADD (Chlamydia protein Associating with Death Domains), a putative folate metabolism enzyme (see TIGR04305).


Pssm-ID: 213919 [Multi-domain]  Cd Length: 208  Bit Score: 160.82  E-value: 1.32e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    14 ESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFAA 93
Cdd:TIGR04306   2 KPIWDDYINHPFVQKIGDGTLERDKFRFYIEQDYAYLVEYAKVHALGGSKACDEDMEKELVEQIQGGVETEMGQHKRLAE 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    94 DYGISQEELEATAK-APTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAEL-TDGTHE-YTPFIDTYTSEEF 170
Cdd:TIGR04306  82 VLGISDEEYFQKIKpGPALRSYTSYMYDVSRRGNWQELVAALLPCLWGYGEAATKLKGKhTAPEHSvYHKWIETYSSSWF 161

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 85680313   171 RGDVAWGRSFVDDCWERYPGE-REAMEEAFIMSAKLEYRFWEMAYT 215
Cdd:TIGR04306 162 REAVNEGENLLNHLAETSSPEeLEKLKTIFAESCEYEYNFWTMAYE 207
TENA_THI-4 pfam03070
TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: ...
 14-215 6.51e-45

TENA/THI-4/PQQC family; Members of this family are found in all the three major phyla of life: archaebacteria, eubacteria, and eukaryotes. In Bacillus subtilis, TENA is one of a number of proteins that enhance the expression of extracellular enzymes, such as alkaline protease, neutral protease and levansucrase. The THI-4 protein, which is involved in thiamine biosynthesis, is also a member of this family. The C-terminal part of these proteins consistently show significant sequence similarity to TENA proteins. This similarity was first noted with the Neurospora crassa THI-4. This family includes bacterial coenzyme PQQ synthesis protein C or PQQC proteins. Pyrroloquinoline quinone (PQQ) is the prosthetic group of several bacterial enzymes,including methanol dehydrogenase of methylotrophs and the glucose dehydrogenase of a number of bacteria. PQQC has been found to be required in the synthesis of PQQ but its function is unclear. The exact molecular function of members of this family is uncertain.


Pssm-ID: 397272 [Multi-domain]  Cd Length: 210  Bit Score: 148.66  E-value: 6.51e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    14 ESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFAA 93
Cdd:pfam03070   5 RPIWKFYINHPFVQALAKGTLPREQFQGYVIQDYLYLVNFARVLAIIASKSPDLEVRREWENRIVDHDGNEIELHLRLAE 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    94 DYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE-LTDGTH-EYTPFIDTYTSEEFR 171
Cdd:pfam03070  85 ALGLSREDLSAYKPLPATRAYVRYLLDFARRGSWLEAVAALLPCLFVYQEIASRLGEkIRALEGpEYYEWVKTYASEYFR 164

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 85680313   172 GDVAWGRSFVDDCWERYPGER-EAMEEAFIMSAKLEYRFWEMAYT 215
Cdd:pfam03070 165 AAVEEAKRLLDRLLAYVSDEQlEELQEIFLKALEFEYSFWTMALD 209
TenA_C_SaTenA-like cd19360
TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins ...
 6-212 1.62e-42

TenA_C proteins similar to Staphylococcus aureus TenA (SaTenA); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. This family includes Staphylococcus aureus TenA (SaTenA) which plays two essential roles in thiamin metabolism: in the deamination of aminopyrimidine to HMP, and in hydrolyzing thiamin into HMP and 5-(2-hydroxyethyl)4-methylthiazole (THZ). It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. SaTenA is then also a putative transcriptional regulator controlling the secretion of extracellular proteases such as subtilisin-type proteases in bacteria. This family includes mostly uncharacterized TenA like proteins.


Pssm-ID: 381695 [Multi-domain]  Cd Length: 211  Bit Score: 142.73  E-value: 1.62e-42
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKA--REEMRMtaLLDVAHTILET 83
Cdd:cd19360   2 SEELREEAQPILEAIYAHPFVQGIAAGELPKEALIHYVQQDYEYLNAFLKVYALAIAKSdtREDMRF--FLEQIGFILNG 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  84 EMDLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAE--LTDGTHEYTPF 161
Cdd:cd19360  80 ESHAHQNLCEVAGVDYEELQGAPWAPTADHYIKHMYYAARTGDLGDILAALLPCPWTYVELAKRLIEegKPTPDNPFYEW 159

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|..
gi 85680313 162 IDTYTSEEFRGDVAWGRSFVDDCWERY-PGEREAMEEAFIMSAKLEYRFWEM 212
Cdd:cd19360 160 IDFYADDEMDGLTDQLFARLDRLAEKAsEEERERAKQAFLKSCQLEWRFWEM 211
TenA_C_PH1161-like cd19363
uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA ...
 6-214 2.00e-41

uncharacterized TenA_C proteins similar to Pyrococcus horikoshii PH1161; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes functionally uncharacterized TenA like proteins such as Pyrococcus horikoshii PH1161 protein.


Pssm-ID: 381698 [Multi-domain]  Cd Length: 210  Bit Score: 139.77  E-value: 2.00e-41
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19363   2 TGKLRRDADDIWKKILNHPFVVELYSGTLPMEKFKFYLLQDYNYLVGSTKNLSILASKAESLDLMRELLELAYGEATTEF 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DLHRSFAADYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAEL--TDGTHEYTPFID 163
Cdd:cd19363  82 ANYEELLDELGLSLEDAIKVEPFPTNVAYMNFLLSTSSLGSFYEGLAALLPCFWSYLEIAEYHKDKlsENPNDIYRDWAS 161

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|.
gi 85680313 164 TYTSEEFRGDVAWGRSFVDDCWERYPGEReaMEEAFIMSAKLEYRFWEMAY 214
Cdd:cd19363 162 VYLSKEYKELVERLRRIVDKYGEGEPFEK--LKRIFKTASKYEYMFWDAAY 210
TenA_C-like cd19369
uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as ...
 16-212 1.65e-40

uncharacterized TenA_C proteins; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA_C- like proteins.


Pssm-ID: 381704 [Multi-domain]  Cd Length: 202  Bit Score: 137.36  E-value: 1.65e-40
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  16 IWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAR--EEMRMtaLLDVAHTILETEMDLHRSFAA 93
Cdd:cd19369   2 IWEQYLEHPFIKELGEGTLDKEKFKNYLIQDSLYLKEYAKVFAMGIYKSRtmKEMQF--FYSSLSFVNEDETATRIKYLK 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  94 DYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDG---THEYTPFIDTYTSEEF 170
Cdd:cd19369  80 EFGLTDEDIEKIEPLPENKAYTDYMLGIAKTGDVKEILMAVLPCMLSYYYIFKELVKKYKDnleSNPYKDWIEDYASEEY 159

                       170       180       190       200
                ....*....|....*....|....*....|....*....|...
gi 85680313 171 RGDVAWGRSFVDDCWERYPGER-EAMEEAFIMSAKLEYRFWEM 212
Cdd:cd19369 160 AEYCKEWIDFADRLCENLSEEEkEKLKEIFRKASLYELKFWDM 202
PRK14713 PRK14713
bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;
6-213 2.55e-35

bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase;


Pssm-ID: 237797 [Multi-domain]  Cd Length: 530  Bit Score: 130.68  E-value: 2.55e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313    6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:PRK14713 321 TAALWQASGPIREAIEDLPFVRALADGTLPEEAFEFYLAQDALYLNGYSRALARLAALAPDPAEQVFWAQSAQACLEVES 400

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   86 DLHRSFAADYGISqeeleaTAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIAdhmAELTD-----GTHEYTP 160
Cdd:PRK14713 401 ELHRSWLGDRDAD------TAPSPVTLAYTDFLLARAAGGSYAVGAAAVLPCFWLYAEVG---AELHAragnpDDHPYAE 471

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....
gi 85680313  161 FIDTYTSEEFRGDVAWGRSFVDDCWER-YPGEREAMEEAFIMSAKLEYRFWEMA 213
Cdd:PRK14713 472 WLQTYADPEFAAATRRAIAFVDRAFRAaSPAERAAMARAFLTACRYELEFFDQA 525
TenA_C_AtTH2-like cd19368
TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine ...
 23-170 2.03e-34

TenA_C family similar to the N-terminal TenA_C domain of Arabidopsis thaliana thiamine requiring 2; This TenA family belongs to the TenA_C class as it has a conserved active site Cys residue; the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. Arabidopsis thaliana TH2 is an orphan enzyme thiamin monophosphate phosphatase which has a haloacid dehalogenase (HAD) family domain fused to its TenA_C domain, it's TenA_C domain has thiamin salvage hydrolase activity against amino-HMP. This family includes mostly uncharacterized single-domain TenA_C- like proteins; some however have additional domains such as a HAD family domain or a kinase domain It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381703 [Multi-domain]  Cd Length: 210  Bit Score: 121.96  E-value: 2.03e-34
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  23 HPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILEtEMDLHRSFAADYGIsqEEL 102
Cdd:cd19368  19 HPFVVGLAAGNLPLDSFRHYISQDAHFLEAFARAYELAEAKADDDEDKKAIRELRKGVLE-ELKLHDSYAEEWGV--DLP 95

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 85680313 103 EATAKAPTCVAYTNFLLRTA--YEGSIAEIAAALYPCMQGYLDIADHMAELTDGT--HEYTPFIDTYTSEEF 170
Cdd:cd19368  96 KEVTPDPATRKYTDFLLATAsgKVKVAAYTLAAMAPCMRLYAFLGQELARALDDTedHPYKKWIDTYSSQEF 167
TenA_E_At3g16990-like cd19357
TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins ...
 7-212 2.16e-31

TenA_E proteins similar to Arabidopsis thaliana At3g16990; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Members of this family include Arabidopsis thaliana At3g16990, Zea mays GRMZM2G080501, and Pyrococcus furiosus PF1337, among others. Arabidopsis thaliana TenA_E hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP, but does not hydrolyze thiamin; nor does it have activity with other thiamine degradation products such as thiamine mono- or diphosphate, oxythiamine, oxothiamine, thiamine disulfide, desthiothiamine or thiochrome as substrates. Structural studies of P. furiosus PF1337 strongly support its enzymatic function in thiamine biosynthesis. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect.


Pssm-ID: 381692 [Multi-domain]  Cd Length: 217  Bit Score: 114.34  E-value: 2.16e-31
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   7 DHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAR-----EEMRMTALLDVAHTIL 81
Cdd:cd19357   2 SHLLSLHPALYTAATQHPFLRAAADGTLPKEALSRWLAQDRLYVQAYIRFLGSLLARAPlpsssLNQRLLDVLLGALANL 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  82 ETEMDLHRSFAADYGISQEELEAtAKAPTCVAYTNFLLRTAYEG-SIAEIAAALYPCMQGYLD----IADHMAELTDGTH 156
Cdd:cd19357  82 RRELAFFEETAAEYGLDLPGLGV-PPSPATRAYVDFLASLASEGvSYLEGLVVLWATEKVYLDawsyARSFLPSDADGGA 160

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 85680313 157 EYTPFIDTYTSEEFRGDVAWGRSFVDDCWERYPGE-REAMEEAFIMSAKLEYRFWEM 212
Cdd:cd19357 161 LYREFIPNWTSPEFAAFVDRLGDLVDEALEQAGEEvLERAEEVWRRVLELEEAFWPM 217
TenA_E_Spr0628-like cd19358
TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C ...
 6-214 7.52e-31

TenA_E proteins similar to Streptococcus pneumoniae Spr0628 and Saccharomyces cerevisiae S288C PET18; This family of TenA proteins belongs to the TenA_E class, and lacks the conserved active site Cys residue of the TenA_C class; most have a pair of structurally conserved Glu residues in the active site. TenA_C proteins (EC 3.5.99.2; aminopyrimidine aminohydrolase, also known as thiaminase II) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway; the role of TenA_E proteins is less clear. Arabidopsis thaliana TenA_E (not belonging to this family) hydrolyzes amino-HMP to AMP, and the N-formyl derivative of amino-HMP to amino-HMP. Members of this family include the putative thiaminase Streptococcus pneumoniae Spr0628, and Saccharomyces cerevisiae S288C PET18, a protein of unknown function whose expression is induced in the absence of thiamin. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. Many proteins in this family have yet to be characterized.


Pssm-ID: 381693 [Multi-domain]  Cd Length: 209  Bit Score: 112.66  E-value: 7.52e-31
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEM 85
Cdd:cd19358   2 SDRLRAANAEDWDAAVTHRFVRELCAGTLPDAVLARYLVQDYQFVETFLRLLGKAVAKAPDLEAKLRLARFLGFLANDEN 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  86 DL-HRSFAAdYGISQEELEATAKAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHEYTPFIDT 164
Cdd:cd19358  82 DYfERAFAA-LGVSEADREAPPLLPATRAFIDLMLEAARSGSYAEILTVLLVAEWLYLDWASRAAAAAPLRFKHQEWIDL 160

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|
gi 85680313 165 YTSEEFRGDVAWGRSFVDDCWERyPGEREAMEEAFIMSAKLEYRFWEMAY 214
Cdd:cd19358 161 HSGPEFEAWVDFLRDEVDRVGPT-EEERERLEAVFARAVELEIAFFDAAY 209
PTZ00347 PTZ00347
phosphomethylpyrimidine kinase; Provisional
 23-219 4.88e-25

phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 240375 [Multi-domain]  Cd Length: 504  Bit Score: 101.96  E-value: 4.88e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   23 HPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFAadygisqEEL 102
Cdd:PTZ00347  31 LPFVQGLGDGTLDQNAFRTYIAQDTLYLNGYIRILSYCITKSDVTATGGGLLELLKGVLEELKNCHHHYI-------DNP 103

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  103 EATAKAPTCVAYTNFLL---RTAYEGSIAEIAAALyPCMQGYLDIADHMA---ELTDgTHEYTPFIDTYTSEEFRGDVAW 176
Cdd:PTZ00347 104 DAAGPEAACRKYVDFLLasgNADTLGPSVVIAAVI-PCARLYAWVGQELTnevELTE-SHPFRRWLLSYSDEPINTSVEQ 181

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 85680313  177 GRSFVDDcwERYPGEREAMEEAFIMSAKLEYRFWEMAYTTEGW 219
Cdd:PTZ00347 182 LESLLDK--YIRPGEFSEVAQAYRRAMELEYDFFDSFGYCLGR 222
PRK09517 PRK09517
multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; ...
 24-220 4.14e-22

multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase; Provisional


Pssm-ID: 169939 [Multi-domain]  Cd Length: 755  Bit Score: 93.89  E-value: 4.14e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   24 PFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKAREEMRMTALLDVAHTILETEMDLHRSFaadygISQEELE 103
Cdd:PRK09517 565 DFIRMLGDGTLRRPEFDFYIDQDAQYLRQYSRALARLSSIAPDSHAQVEWAQSAAECIVVEAELHRSY-----LSGKEAP 639

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  104 ATAkAPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAELTDGTHEYTPFIDTYTSEEFRGDVAWGRSFVDD 183
Cdd:PRK09517 640 SAP-SPVTMAYTDFLIARTYTEDYVVGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARVEK 718

                        170       180       190
                 ....*....|....*....|....*....|....*...
gi 85680313  184 CWERY-PGEREAMEEAFIMSAKLEYRFWEMAyTTEGWD 220
Cdd:PRK09517 719 ALENAgPEQRVDAARAFLSASVHEREFFDQA-TRHGWT 755
TenA_C_Bt3146-like cd19359
uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of ...
 7-211 2.11e-14

uncharacterized TenA_C proteins similar to Bacteroides thetaiotaomicron Bt3146; This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA-like proteins such as Bacteroides thetaiotaomicron Bt3146.


Pssm-ID: 381694 [Multi-domain]  Cd Length: 206  Bit Score: 68.91  E-value: 2.11e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   7 DHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARLWALAGTKA-------REEMRMTALLDVAHT 79
Cdd:cd19359   2 DKLWNDNEDLWDKALNNPFCQGMADGTLDLDGFGYYMVQDYYYCINYVRFKALRAAKApdpdllaFLAAKIKSYLDYAED 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  80 ILETemdLHRSFAADYGISQEELEatakaPTCVAYTNFLLRTAYEGSIAEIAAALYPCMQGYLDIADHMAEltDGTHEYT 159
Cdd:cd19359  82 FLKT---CHIKLGIPDVVDGVKPS-----PALKAYVDFERSVAESEDWFYLLVAMIPCIYLWYWLANQLNE--DPSDKNT 151

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....
gi 85680313 160 PFIDTYTSEEFRGDVAWG--RSFVDDCWERYPGEREAMEEAFIMSAKLEYRFWE 211
Cdd:cd19359 152 NFYKTWIEPNLPDPSSAKqlEFFLNANAAWSKIDREKANEIFRQAMQLEINFFN 205
TenA_C_SsTenA-1-like cd19362
uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This ...
 6-213 4.61e-14

uncharacterized TenA_C proteins similar to Sulfolobus solfataricus TenA-1 (Sso2206); This family of TenA proteins belongs to the TenA_C class as it has a conserved active site Cys residue; only one of the double Glu residues identified in the active site of TenA_E from the archaeon Pyrococcus furiosus is conserved in this family. TenA_C proteins (EC 3.5.99.2) catalyze the hydrolysis of the thiamin breakdown product 4-amino-5-amino-methyl-2-methylpyrimidine (amino-HMP) to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) in a thiamin salvage pathway. It has also been suggested that TenA proteins act as transcriptional regulators based on changes in gene-expression patterns when TenA is overexpressed in Bacillus subtilis, however this effect may be indirect. This family includes mostly uncharacterized TenA like proteins such as Sulfolobus solfataricus putative TenA-like thiaminase (Tena-1, Sso2206).


Pssm-ID: 381697 [Multi-domain]  Cd Length: 200  Bit Score: 67.85  E-value: 4.61e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313   6 SDHLLDAGESIWQAQKTHPFVVELADGTLDREAFEHWVKQDYRYLQDYARlwalAGTKAREEMRMTALLDVAHTILETE- 84
Cdd:cd19362   1 SENLKNLVGDLWNKYVRHEFVERMRDGTLPLDNFRYYLIQDSKYVEEMLR----ALLRASSKAPLDKAIKILNSVFSGRd 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 85680313  85 --MDLHRSFAADYGISQEELEATAKAPTCVAYTNFLLrtaYEGSIA--EIAAALYPCMQGYLDIADHMAELTDgtHEYTP 160
Cdd:cd19362  77 kgMEVHKFLYSELGITEDEIRRTGYNLVNYAYTRHLY---YYSTLGwpQFLAAWAPCMWGYSEIGKYVLNSPN--ELYKT 151

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|...
gi 85680313 161 FIDTYTSEEFRGDVawgrSFVDDCWERYPgEREAMEEAFIMSAKLEYRFWEMA 213
Cdd:cd19362 152 WASFYASKDYKKRV----EAILEALDSIE-DTEDIKNIFRNSVNFEIMFWDAA 199
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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