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Conserved domains on  [gi|663528511|gb|AIF18548|]
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Fe-S oxidoreductase [uncultured marine thaumarchaeote KM3_83_D12]

Protein Classification

radical SAM protein( domain architecture ID 11425241)

radical SAM protein generates radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity; contains a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster; transfers a single electron from the iron-sulfur cluster to SAM leads to its reductive cleavage to methionine and a 5'-deoxyadenosyl radical

Gene Ontology:  GO:1904047|GO:0051539|GO:0003824
PubMed:  11222759|18307109|17936058
SCOP:  3000308

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SkfB COG0535
Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, ...
 42-158 2.63e-29

Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, chromosome partitioning, Coenzyme transport and metabolism];


:

Pssm-ID: 440301 [Multi-domain]  Cd Length: 159  Bit Score: 109.61  E-value: 2.63e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  42 DIINECNLHCEHCYWWLNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELFT--KEFPKRSCVVTNG- 118
Cdd:COG0535    5 ELTNRCNLRCKHCYADAGPKRPGELSTEEAKRILDE-LAELGVKVVGLTGGEPLLRPDLFELVEyaKELGIRVNLSTNGt 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 663528511 119 ---NYPLKKFKD--LYFYWVSIDG-DQKIHDTVRG-DGTWAKTRKNV 158
Cdd:COG0535   84 lltEELAERLAEagLDHVTISLDGvDPETHDKIRGvPGAFDKVLEAI 130
 
Name Accession Description Interval E-value
SkfB COG0535
Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, ...
 42-158 2.63e-29

Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, chromosome partitioning, Coenzyme transport and metabolism];


Pssm-ID: 440301 [Multi-domain]  Cd Length: 159  Bit Score: 109.61  E-value: 2.63e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  42 DIINECNLHCEHCYWWLNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELFT--KEFPKRSCVVTNG- 118
Cdd:COG0535    5 ELTNRCNLRCKHCYADAGPKRPGELSTEEAKRILDE-LAELGVKVVGLTGGEPLLRPDLFELVEyaKELGIRVNLSTNGt 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 663528511 119 ---NYPLKKFKD--LYFYWVSIDG-DQKIHDTVRG-DGTWAKTRKNV 158
Cdd:COG0535   84 lltEELAERLAEagLDHVTISLDGvDPETHDKIRGvPGAFDKVLEAI 130
Radical_SAM cd01335
Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S ...
 47-235 2.95e-11

Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster. Mechanistically, they share the transfer of a single electron from the iron-sulfur cluster to SAM, which leads to its reductive cleavage to methionine and a 5'-deoxyadenosyl radical, which, in turn, abstracts a hydrogen from the appropriately positioned carbon atom. Depending on the enzyme, SAM is consumed during this process or it is restored and reused. Radical SAM enzymes catalyze steps in metabolism, DNA repair, the biosynthesis of vitamins and coenzymes, and the biosynthesis of many antibiotics. Examples are biotin synthase (BioB), lipoyl synthase (LipA), pyruvate formate-lyase (PFL), coproporphyrinogen oxidase (HemN), lysine 2,3-aminomutase (LAM), anaerobic ribonucleotide reductase (ARR), and MoaA, an enzyme of the biosynthesis of molybdopterin.


Pssm-ID: 100105 [Multi-domain]  Cd Length: 204  Bit Score: 61.97  E-value: 2.95e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHCY-WWLNRKENEELTLEEWKQVIDEKFKKRHVFAVTVVGGEPMMRPDVVELFT---KEFPKRS-CVVTNGNYP 121
Cdd:cd01335    7 CNLNCGFCSnPASKGRGPESPPEIEEILDIVLEAKERGVEVVILTGGEPLLYPELAELLRrlkKELPGFEiSIETNGTLL 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511 122 ----LKKFKDLYFYWVSI---DGDQKIHDTVRGDGTWAKTRKNVIDYVEnngsKAYKDIWISMTINsRNYktvrkVIEDW 194
Cdd:cd01335   87 teelLKELKELGLDGVGVsldSGDEEVADKIRGSGESFKERLEALKELR----EAGLGLSTTLLVG-LGD-----EDEED 156

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 663528511 195 REYSNKIGVQFHTPFMKGDPLWLPFGEERNAVVDELIDLQK 235
Cdd:cd01335  157 DLEELELLAEFRSPDRVSLFRLLPEEGTPLELAAPVVPAEK 197
SCM_rSAM_ScmE TIGR04250
SynChlorMet cassette radical SAM/SPASM protein ScmE; A biosynthesis cassette found in ...
 42-158 1.26e-09

SynChlorMet cassette radical SAM/SPASM protein ScmE; A biosynthesis cassette found in Syntrophobacter fumaroxidans MPOB, Chlorobium limicola DSM 245, Methanocella paludicola SANAE, and delta proteobacterium NaphS2 contains two PqqE-like radical SAM/SPASM domain proteins, a PqqD homolog, and a conserved hypothetical protein. These components suggest modification of a ribosomally produced peptide precursor, but the precursor has not been identified. Of the two PqqE homologs of the cassette, this family is the closer in sequence.


Pssm-ID: 211973 [Multi-domain]  Cd Length: 358  Bit Score: 58.71  E-value: 1.26e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   42 DIINECNLHCEHCYWWLNRKE-NEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELFTKEFPKRS--CVVTNG 118
Cdd:TIGR04250   8 DITGRCNLRCRYCSHFSSAAEtPTDLETAEWLRFFRE-LNRCSVLRVVLSGGEPFMRSDFREIIDGIVKNRMrfSILSNG 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 663528511  119 NY-------PLKKFKDLYFYWVSIDGDQ-KIHDTVRGDGTWAKTRKNV 158
Cdd:TIGR04250  87 TLitdaiasFLAATRRCDYVQVSIDGSTpGTHDRLRGTGSFLQAVEGI 134
Radical_SAM pfam04055
Radical SAM superfamily; Radical SAM proteins catalyze diverse reactions, including unusual ...
 43-173 5.77e-09

Radical SAM superfamily; Radical SAM proteins catalyze diverse reactions, including unusual methylations, isomerization, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation.


Pssm-ID: 427681 [Multi-domain]  Cd Length: 159  Bit Score: 54.45  E-value: 5.77e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   43 IINECNLHCEHCY--WWLNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELF------TKEFPKRSCV 114
Cdd:pfam04055   1 ITRGCNLRCTYCAfpSIRARGKGRELSPEEILEEAKE-LKRLGVEVVILGGGEPLLLPDLVELLerllklELAEGIRITL 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 663528511  115 VTNGNYP----LKKFKDLYFYWVSID---GDQKIHDTVRGDGTWAKtRKNVIDYVENNGSKAYKDI 173
Cdd:pfam04055  80 ETNGTLLdeelLELLKEAGLDRVSIGlesGDDEVLKLINRGHTFEE-VLEALELLREAGIPVVTDN 144
moaA PRK00164
GTP 3',8-cyclase MoaA;
43-125 5.84e-05

GTP 3',8-cyclase MoaA;


Pssm-ID: 234672 [Multi-domain]  Cd Length: 331  Bit Score: 43.98  E-value: 5.84e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  43 IINECNLHCEHC-----YWWLNRKEneELTLEEWKQVIdekfkkrHVFA---VTVV---GGEPMMRPDVVELF--TKEFP 109
Cdd:PRK00164  23 VTDRCNFRCTYCmpegyLPFLPKEE--LLSLEEIERLV-------RAFValgVRKVrltGGEPLLRKDLEDIIaaLAALP 93

                         90
                 ....*....|....*...
gi 663528511 110 KRS--CVVTNGnYPLKKF 125
Cdd:PRK00164  94 GIRdlALTTNG-YLLARR 110
 
Name Accession Description Interval E-value
SkfB COG0535
Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, ...
 42-158 2.63e-29

Radical SAM superfamily maturase, SkfB/NifB/PqqE family [Cell cycle control, cell division, chromosome partitioning, Coenzyme transport and metabolism];


Pssm-ID: 440301 [Multi-domain]  Cd Length: 159  Bit Score: 109.61  E-value: 2.63e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  42 DIINECNLHCEHCYWWLNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELFT--KEFPKRSCVVTNG- 118
Cdd:COG0535    5 ELTNRCNLRCKHCYADAGPKRPGELSTEEAKRILDE-LAELGVKVVGLTGGEPLLRPDLFELVEyaKELGIRVNLSTNGt 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 663528511 119 ---NYPLKKFKD--LYFYWVSIDG-DQKIHDTVRG-DGTWAKTRKNV 158
Cdd:COG0535   84 lltEELAERLAEagLDHVTISLDGvDPETHDKIRGvPGAFDKVLEAI 130
AslB COG0641
Sulfatase maturation enzyme AslB, radical SAM superfamily [Posttranslational modification, ...
 47-234 2.77e-17

Sulfatase maturation enzyme AslB, radical SAM superfamily [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440406 [Multi-domain]  Cd Length: 349  Bit Score: 81.19  E-value: 2.77e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHCY-WWLNRKENEELTLEEWKQVID---EKFKKRHVFAVTVVGGEPMMRPD----VVELFTKEFPK----RSCV 114
Cdd:COG0641   11 CNLRCSYCYySEGDEGSRRRMSEETAEKAIDfliESSGPGKELTITFFGGEPLLNFDfikeIVEYARKYAKKgkkiRFSI 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511 115 VTNG----NYPLKKFKDLYFYW-VSIDGDQKIHDTVR----GDGTWAKTRKNvIDYVENNGSKAYkdiwISMTINSRNYK 185
Cdd:COG0641   91 QTNGtlldDEWIDFLKENGFSVgISLDGPKEIHDRNRvtknGKGSFDRVMRN-IKLLKEHGVEVN----IRCTVTRENLD 165

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 663528511 186 TVRKVIEDWREY-SNKIGVQFHTPFMKGDPLWLP--FGEERNAVVDELIDLQ 234
Cdd:COG0641  166 DPEELYDFLKELgFRSIQFNPVVEEGEADYSLTPedYGEFLIELFDEWLERD 217
Radical_SAM cd01335
Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S ...
 47-235 2.95e-11

Radical SAM superfamily. Enzymes of this family generate radicals by combining a 4Fe-4S cluster and S-adenosylmethionine (SAM) in close proximity. They are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster. Mechanistically, they share the transfer of a single electron from the iron-sulfur cluster to SAM, which leads to its reductive cleavage to methionine and a 5'-deoxyadenosyl radical, which, in turn, abstracts a hydrogen from the appropriately positioned carbon atom. Depending on the enzyme, SAM is consumed during this process or it is restored and reused. Radical SAM enzymes catalyze steps in metabolism, DNA repair, the biosynthesis of vitamins and coenzymes, and the biosynthesis of many antibiotics. Examples are biotin synthase (BioB), lipoyl synthase (LipA), pyruvate formate-lyase (PFL), coproporphyrinogen oxidase (HemN), lysine 2,3-aminomutase (LAM), anaerobic ribonucleotide reductase (ARR), and MoaA, an enzyme of the biosynthesis of molybdopterin.


Pssm-ID: 100105 [Multi-domain]  Cd Length: 204  Bit Score: 61.97  E-value: 2.95e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHCY-WWLNRKENEELTLEEWKQVIDEKFKKRHVFAVTVVGGEPMMRPDVVELFT---KEFPKRS-CVVTNGNYP 121
Cdd:cd01335    7 CNLNCGFCSnPASKGRGPESPPEIEEILDIVLEAKERGVEVVILTGGEPLLYPELAELLRrlkKELPGFEiSIETNGTLL 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511 122 ----LKKFKDLYFYWVSI---DGDQKIHDTVRGDGTWAKTRKNVIDYVEnngsKAYKDIWISMTINsRNYktvrkVIEDW 194
Cdd:cd01335   87 teelLKELKELGLDGVGVsldSGDEEVADKIRGSGESFKERLEALKELR----EAGLGLSTTLLVG-LGD-----EDEED 156

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 663528511 195 REYSNKIGVQFHTPFMKGDPLWLPFGEERNAVVDELIDLQK 235
Cdd:cd01335  157 DLEELELLAEFRSPDRVSLFRLLPEEGTPLELAAPVVPAEK 197
SCM_rSAM_ScmE TIGR04250
SynChlorMet cassette radical SAM/SPASM protein ScmE; A biosynthesis cassette found in ...
 42-158 1.26e-09

SynChlorMet cassette radical SAM/SPASM protein ScmE; A biosynthesis cassette found in Syntrophobacter fumaroxidans MPOB, Chlorobium limicola DSM 245, Methanocella paludicola SANAE, and delta proteobacterium NaphS2 contains two PqqE-like radical SAM/SPASM domain proteins, a PqqD homolog, and a conserved hypothetical protein. These components suggest modification of a ribosomally produced peptide precursor, but the precursor has not been identified. Of the two PqqE homologs of the cassette, this family is the closer in sequence.


Pssm-ID: 211973 [Multi-domain]  Cd Length: 358  Bit Score: 58.71  E-value: 1.26e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   42 DIINECNLHCEHCYWWLNRKE-NEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELFTKEFPKRS--CVVTNG 118
Cdd:TIGR04250   8 DITGRCNLRCRYCSHFSSAAEtPTDLETAEWLRFFRE-LNRCSVLRVVLSGGEPFMRSDFREIIDGIVKNRMrfSILSNG 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 663528511  119 NY-------PLKKFKDLYFYWVSIDGDQ-KIHDTVRGDGTWAKTRKNV 158
Cdd:TIGR04250  87 TLitdaiasFLAATRRCDYVQVSIDGSTpGTHDRLRGTGSFLQAVEGI 134
PflA COG1180
Pyruvate-formate lyase-activating enzyme [Posttranslational modification, protein turnover, ...
 47-179 3.71e-09

Pyruvate-formate lyase-activating enzyme [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440793 [Multi-domain]  Cd Length: 242  Bit Score: 56.35  E-value: 3.71e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHCY-WWL-NRKENEELTLEEWKQVIDEkFKKRHVF-----AVTVVGGEPMMRPD-VVELFT--KEFPKRSCVVT 116
Cdd:COG1180   31 CNLRCPYCHnPEIsQGRPDAAGRELSPEELVEE-ALKDRGFldscgGVTFSGGEPTLQPEfLLDLAKlaKELGLHTALDT 109

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 663528511 117 NGNYPLKKFKDLYFY--WVSID---GDQKIHDTVRGdGTWAKTRKNvIDYVENNGskayKDIWISMTI 179
Cdd:COG1180  110 NGYIPEEALEELLPYldAVNIDlkaFDDEFYRKLTG-VSLEPVLEN-LELLAESG----VHVEIRTLV 171
Radical_SAM pfam04055
Radical SAM superfamily; Radical SAM proteins catalyze diverse reactions, including unusual ...
 43-173 5.77e-09

Radical SAM superfamily; Radical SAM proteins catalyze diverse reactions, including unusual methylations, isomerization, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation.


Pssm-ID: 427681 [Multi-domain]  Cd Length: 159  Bit Score: 54.45  E-value: 5.77e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   43 IINECNLHCEHCY--WWLNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMMRPDVVELF------TKEFPKRSCV 114
Cdd:pfam04055   1 ITRGCNLRCTYCAfpSIRARGKGRELSPEEILEEAKE-LKRLGVEVVILGGGEPLLLPDLVELLerllklELAEGIRITL 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 663528511  115 VTNGNYP----LKKFKDLYFYWVSID---GDQKIHDTVRGDGTWAKtRKNVIDYVENNGSKAYKDI 173
Cdd:pfam04055  80 ETNGTLLdeelLELLKEAGLDRVSIGlesGDDEVLKLINRGHTFEE-VLEALELLREAGIPVVTDN 144
Fer4_12 pfam13353
4Fe-4S single cluster domain; This family includes proteins containing domains which bind to ...
 47-118 1.97e-07

4Fe-4S single cluster domain; This family includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. The structure of the domain is an alpha-antiparallel beta sandwich.


Pssm-ID: 433138 [Multi-domain]  Cd Length: 137  Bit Score: 49.48  E-value: 1.97e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 663528511   47 CNLHCEHCY---WWlNRKENEELTLEEWKQVIDEkFKKRHVFAVTVVGGEPMM-RPDVVELFTK---EFPKRSCVVTNG 118
Cdd:pfam13353  15 CNHHCKGCFnpeTW-DFKYGKPFTEELEDEIIED-LAKPYIQGLTLSGGEPLLnAEALLELVKRvreECPEKDIWLWTG 91
SCM_rSAM_ScmF TIGR04251
SynChlorMet cassette radical SAM/SPASM protein ScmF; A biosynthesis cassette found in ...
 47-191 6.46e-07

SynChlorMet cassette radical SAM/SPASM protein ScmF; A biosynthesis cassette found in Syntrophobacter fumaroxidans MPOB, Chlorobium limicola DSM 245, Methanocella paludicola SANAE, and delta proteobacterium NaphS2 contains two PqqE-like radical SAM/SPASM domain proteins, a PqqD homolog, and a conserved hypothetical protein. These components suggest modification of a ribosomally produced peptide precursor, but the precursor has not been identified. Of the two PqqE homologs of the cassette, this family is the more distant in sequence.


Pssm-ID: 211974 [Multi-domain]  Cd Length: 353  Bit Score: 50.22  E-value: 6.46e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   47 CNLHCEHCywWLNRKENEE------LTLEEWKQVIDEKfKKRHVFAVTVVGGEPMMRPDVVELF--TKEFPKRSCVVTNG 118
Cdd:TIGR04251  14 CNLKCRHC--WIDPKYQGEgeqhpsLDPSLFRSIIRQA-IPLGLTSVKLTGGEPLLHPAIGEILecIGENNLQLSVETNG 90

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  119 NYPLKKF-KDLY-----FYWVSIDG-DQKIHDTVRG-DGTWAKTRKNVIDYVENNGSKAykdiwISMTINSRNYKTVRKV 190
Cdd:TIGR04251  91 LLCTPQTaRDLAscetpFVSVSLDGvDAATHDWMRGvKGAFDKAVRGIHNLVEAGIHPQ-----IIMTVTRRNVGQMEQI 165


                  .
gi 663528511  191 I 191
Cdd:TIGR04251 166 V 166
QueE COG0602
Organic radical activating enzyme NrdG/QueE [Coenzyme transport and metabolism]; Organic ...
 47-136 1.05e-05

Organic radical activating enzyme NrdG/QueE [Coenzyme transport and metabolism]; Organic radical activating enzyme NrdG/QueE is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440367 [Multi-domain]  Cd Length: 205  Bit Score: 45.51  E-value: 1.05e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHC---YWWlNRKENEELTLEEwkqvIDEKFKKRHVFAVTVVGGEPMMRPDVVELfTKEFPKRSCVV---TNGNY 120
Cdd:COG0602   30 CNLRCSWCdtkYAW-DGEGGKRMSAEE----ILEEVAALGARHVVITGGEPLLQDDLAEL-LEALKDAGYEValeTNGTL 103

                         90
                 ....*....|....*.
gi 663528511 121 PLKKFKDlyfyWVSID 136
Cdd:COG0602  104 PIPAGID----WVTVS 115
Tyw1 COG0731
Wyosine [tRNA(Phe)-imidazoG37] synthetase, radical SAM superfamily [Translation, ribosomal ...
 45-157 1.47e-05

Wyosine [tRNA(Phe)-imidazoG37] synthetase, radical SAM superfamily [Translation, ribosomal structure and biogenesis]; Wyosine [tRNA(Phe)-imidazoG37] synthetase, radical SAM superfamily is part of the Pathway/BioSystem: tRNA modification


Pssm-ID: 440495 [Multi-domain]  Cd Length: 248  Bit Score: 45.57  E-value: 1.47e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  45 NECNLHCEHCywWLNRKENEELTLEEW---KQVIDE--KFKKRHVFA------VTVVG-GEPMMRPD---VVELFTKEFP 109
Cdd:COG0731   32 KTCNFDCVYC--QRGRTTDLTRERREFddpEEILEEliEFLRKLPEEarepdhITFSGsGEPTLYPNlgeLIEEIKKLRG 109

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 663528511 110 KRSCVVTNG---NYP-----LKKFKDLYFywvSID-GD----QKIHDTVRGDgTWAKTRKN 157
Cdd:COG0731  110 IKTALLTNGsllHRPevreeLLKADQVYP---SLDaADeetfRKINRPHPGL-SWERIIEG 166
moaA PRK00164
GTP 3',8-cyclase MoaA;
43-125 5.84e-05

GTP 3',8-cyclase MoaA;


Pssm-ID: 234672 [Multi-domain]  Cd Length: 331  Bit Score: 43.98  E-value: 5.84e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  43 IINECNLHCEHC-----YWWLNRKEneELTLEEWKQVIdekfkkrHVFA---VTVV---GGEPMMRPDVVELF--TKEFP 109
Cdd:PRK00164  23 VTDRCNFRCTYCmpegyLPFLPKEE--LLSLEEIERLV-------RAFValgVRKVrltGGEPLLRKDLEDIIaaLAALP 93

                         90
                 ....*....|....*...
gi 663528511 110 KRS--CVVTNGnYPLKKF 125
Cdd:PRK00164  94 GIRdlALTTNG-YLLARR 110
rSAM_Cxxx_rpt TIGR04115
radical SAM peptide maturase, CXXX-repeat target family; Members of this radical SAM domain ...
 43-169 8.27e-05

radical SAM peptide maturase, CXXX-repeat target family; Members of this radical SAM domain protein are predicted peptide maturases, similar to PqqE, AlbA, the mycofactocin radical SAM maturase, and many others that share the peptide modification radical SAM protein C-terminal additional 4Fe4S-binding domain (TIGR04085). Members co-occur with a protein of unknown function that may be a chaperone or immunity protein and with a peptide that may have twelve or more cysteines occurring regularly spaced every fourth residue. These Cys residues tend to be flanked by residues with small side chains that provide minimal steric hindrance to crosslink formation by the radical SAM enzyme as in the subtilosin A system.


Pssm-ID: 200366 [Multi-domain]  Cd Length: 359  Bit Score: 43.72  E-value: 8.27e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511   43 IINECNLHCEHCYwWLNRKENEELTLEEWKQVIDEKFKKRHVFA-----VTVVGGEPMMRPDVV---------ELFTKEF 108
Cdd:TIGR04115   8 VTDDCQLACKYCY-QTGKNKNKRMSFETAKKAVDYILSGNKGFGepsviWDFIGGEPLLEIELIdricdyiknRMIELNH 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  109 P----KRSCVVTNG-NYP-------LKKFKDLYFYWVSIDGDQKIHDTVR----GDGTWAKTRKNVIDYVE---NNGSKA 169
Cdd:TIGR04115  87 PwfnsYRFSFSTNGvCYFeekvqrfIQKNNQHLSISITIDGTKEKHDSCRvfpdGRGSYDLVVSNAPLWLNqfpYASTKV 166
PRK13745 PRK13745
anaerobic sulfatase-maturation protein;
47-146 1.52e-04

anaerobic sulfatase-maturation protein;


Pssm-ID: 237489 [Multi-domain]  Cd Length: 412  Bit Score: 42.93  E-value: 1.52e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 663528511  47 CNLHCEHCYWWlnrkENEELTLEEWKQVID----EKFKKRHVFAVTVV-------GGEPMMRP-----DVVELFTKEFPK 110
Cdd:PRK13745  24 CNLACDYCYYL----EKSKLYQENPKHVMSdellEKFIKEYINSQTMPqvlftwhGGETLMRPlsfykKALELQKKYARG 99

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 663528511 111 RS---CVVTNGNYPLKK----FKDLYFY-WVSIDGDQKIHDTVR 146
Cdd:PRK13745 100 RQidnCIQTNGTLLTDEwcefFRENNFLvGVSIDGPQEFHDEYR 143
MoaA COG2896
GTP 3',8-cyclase (molybdenum cofactor biosynthesis protein MoaA) [Coenzyme transport and ...
 47-104 3.38e-04

GTP 3',8-cyclase (molybdenum cofactor biosynthesis protein MoaA) [Coenzyme transport and metabolism]; GTP 3',8-cyclase (molybdenum cofactor biosynthesis protein MoaA) is part of the Pathway/BioSystem: Molybdopterin biosynthesis


Pssm-ID: 442141 [Multi-domain]  Cd Length: 329  Bit Score: 41.58  E-value: 3.38e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 663528511  47 CNLHC-----EHCYWWLNRKEneELTLEEWKQVIdekfkkrHVFA---VTVV---GGEPMMRPDVVELF 104
Cdd:COG2896   24 CNFRCtycmpEEGYQFLPKEE--LLSFEEIERLV-------RAFVelgVRKIrltGGEPLLRKDLPELI 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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