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Conserved domains on  [gi|827356527|gb|AKJ78266|]
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neomycin phosphotransferase II [BiFC expression vector kanII-StriRV]

Protein Classification

aminoglycoside 3'-phosphotransferase( domain architecture ID 10790026)

aminoglycoside 3'-phosphotransferase phosphorylates and inactives antibiotic substrates such as kanamycin, streptomycin, neomycin, and gentamicin, among others

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
11-267 2.59e-124

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


:

Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 354.22  E-value: 2.59e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  11 HAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEA 90
Cdd:COG3231    1 GPRLPPALRELLGGYRWEPVTIGESGAKVFRLADGGRPTLYLKIEPAGPAAELEDEADRLRWLAGQGLPVPEVLDFGEDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  91 GRDWLLLGEVPGQDLLSS--HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGL 168
Cdd:COG3231   81 GGAWLLTTAVPGRPAASVseALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGLVDPDDFDEERRGR 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 169 APAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYG 248
Cdd:COG3231  161 PPEELLAELLAERPAEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEGWVEPFLDAYG 240

                        250
                 ....*....|....*....
gi 827356527 249 IaAPDSQRIAFYRLLDEFF 267
Cdd:COG3231  241 I-APDPERLAFYRLLDEFF 258
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
11-267 2.59e-124

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 354.22  E-value: 2.59e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  11 HAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEA 90
Cdd:COG3231    1 GPRLPPALRELLGGYRWEPVTIGESGAKVFRLADGGRPTLYLKIEPAGPAAELEDEADRLRWLAGQGLPVPEVLDFGEDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  91 GRDWLLLGEVPGQDLLSS--HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGL 168
Cdd:COG3231   81 GGAWLLTTAVPGRPAASVseALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGLVDPDDFDEERRGR 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 169 APAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYG 248
Cdd:COG3231  161 PPEELLAELLAERPAEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEGWVEPFLDAYG 240

                        250
                 ....*....|....*....
gi 827356527 249 IaAPDSQRIAFYRLLDEFF 267
Cdd:COG3231  241 I-APDPERLAFYRLLDEFF 258
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 25-267 1.88e-111

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 321.07  E-value: 1.88e-111
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  25 YDWAQQTIGCSDAAVFRLSAqGRPVLFVKTDLSGALNELQDEAARLSWLATTgVPCAAVLDVVTEAGRDWLLLGEVPGQD 104
Cdd:cd05150    1 YRWEPDTIGESGARVYRLDG-GGPVLYLKTAPAGYAYELAREAERLRWLAGK-LPVPEVLDYGSDDGGDWLLTTALPGRD 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 105 LLSSH--LAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMP 182
Cdd:cd05150   79 AASLEplLDPERLVDLLAEALRALHSLPIADCPFDRRLDARLAEARARVEAGLVDEDDFDEERQGRTAEELLAELEATRP 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 183 DGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGE-WADRFLVLYGIAAPDSQRIAFYR 261
Cdd:cd05150  159 AEEDLVVTHGDACLPNIILDPGRFSGFIDLGRLGVADRYQDLALAVRSLRENLGGEeYAERFLDAYGIDAPDPERLAYYR 238


                 ....*.
gi 827356527 262 LLDEFF 267
Cdd:cd05150  239 LLDEFF 244
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 36-254 2.90e-30

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 113.36  E-value: 2.90e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527   36 DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGV-PCAAVLDVVTEA---GRDWLLLGEVPGQDLLSSHLA 111
Cdd:pfam01636  10 SNRTYLVTTGDGRYVLRLPPPGRAAEELRRELALLRHLAAAGVpPVPRVLAGCTDAellGLPFLLMEYLPGEVLARPLLP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  112 PAEKVSI--MADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVV 189
Cdd:pfam01636  90 EERGALLeaLGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAELLDRLEELEERLLAALLALLPAELPPVL 169

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 827356527  190 THGDACLPNIMV-ENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDS 254
Cdd:pfam01636 170 VHGDLHPGNLLVdPGGRVSGVIDFEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYGAFGYAR 235
 
Name Accession Description Interval E-value
Aph COG3231
Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];
11-267 2.59e-124

Aminoglycoside phosphotransferase [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442463 [Multi-domain]  Cd Length: 258  Bit Score: 354.22  E-value: 2.59e-124
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  11 HAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEA 90
Cdd:COG3231    1 GPRLPPALRELLGGYRWEPVTIGESGAKVFRLADGGRPTLYLKIEPAGPAAELEDEADRLRWLAGQGLPVPEVLDFGEDD 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  91 GRDWLLLGEVPGQDLLSS--HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGL 168
Cdd:COG3231   81 GGAWLLTTAVPGRPAASVseALDPERAVELLAEALRRLHALPVADCPFDRRLERRLAEARARVAAGLVDPDDFDEERRGR 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 169 APAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYG 248
Cdd:COG3231  161 PPEELLAELLAERPAEEDLVVTHGDACLPNILVDPGTFSGFIDLGRLGVADRYQDLALAARSLRENLGEGWVEPFLDAYG 240

                        250
                 ....*....|....*....
gi 827356527 249 IaAPDSQRIAFYRLLDEFF 267
Cdd:COG3231  241 I-APDPERLAFYRLLDEFF 258
APH cd05150
Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group ...
 25-267 1.88e-111

Aminoglycoside 3'-phosphotransferase; APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270699 [Multi-domain]  Cd Length: 244  Bit Score: 321.07  E-value: 1.88e-111
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  25 YDWAQQTIGCSDAAVFRLSAqGRPVLFVKTDLSGALNELQDEAARLSWLATTgVPCAAVLDVVTEAGRDWLLLGEVPGQD 104
Cdd:cd05150    1 YRWEPDTIGESGARVYRLDG-GGPVLYLKTAPAGYAYELAREAERLRWLAGK-LPVPEVLDYGSDDGGDWLLTTALPGRD 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 105 LLSSH--LAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMP 182
Cdd:cd05150   79 AASLEplLDPERLVDLLAEALRALHSLPIADCPFDRRLDARLAEARARVEAGLVDEDDFDEERQGRTAEELLAELEATRP 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 183 DGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGE-WADRFLVLYGIAAPDSQRIAFYR 261
Cdd:cd05150  159 AEEDLVVTHGDACLPNIILDPGRFSGFIDLGRLGVADRYQDLALAVRSLRENLGGEeYAERFLDAYGIDAPDPERLAYYR 238


                 ....*.
gi 827356527 262 LLDEFF 267
Cdd:cd05150  239 LLDEFF 244
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 36-254 2.90e-30

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 113.36  E-value: 2.90e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527   36 DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGV-PCAAVLDVVTEA---GRDWLLLGEVPGQDLLSSHLA 111
Cdd:pfam01636  10 SNRTYLVTTGDGRYVLRLPPPGRAAEELRRELALLRHLAAAGVpPVPRVLAGCTDAellGLPFLLMEYLPGEVLARPLLP 89

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  112 PAEKVSI--MADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVV 189
Cdd:pfam01636  90 EERGALLeaLGRALARLHAVDPAALPLAGRLARLLELLRQLEAALARLLAAELLDRLEELEERLLAALLALLPAELPPVL 169

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 827356527  190 THGDACLPNIMV-ENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDS 254
Cdd:pfam01636 170 VHGDLHPGNLLVdPGGRVSGVIDFEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAAYGAFGYAR 235
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 36-267 2.43e-19

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 85.17  E-value: 2.43e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  36 DAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLA-TTGVPCAAVLDVVTEA---GRDWLLLGEVPGQDLLS--SH 109
Cdd:COG3173   33 SNLTYRLDTGDRLVLRRPPRGLASAHDVRREARVLRALApRLGVPVPRPLALGEDGeviGAPFYVMEWVEGETLEDalPD 112

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 110 LAPAEKVSI---MADAMRRLHTLDPATCPFD----HQAKHRIERARTRMEAGLVDQDDLDEehqglAPAELFARLKARMP 182
Cdd:COG3173  113 LSPAERRALaraLGEFLAALHAVDPAAAGLAdgrpEGLERQLARWRAQLRRALARTDDLPA-----LRERLAAWLAANLP 187

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 183 DGEDLVVTHGDACLPNIMV--ENGRFSGFIDCGRLGVADRYQDIA--LATRDIAEELGGEwADRFLVLYGIAAPDSQRIA 258
Cdd:COG3173  188 EWGPPVLVHGDLRPGNLLVdpDDGRLTAVIDWELATLGDPAADLAylLLYWRLPDDLLGP-RAAFLAAYEEATGDLDDLT 266


                 ....*....
gi 827356527 259 FYRLLDEFF 267
Cdd:COG3173  267 WWALADPEL 275
ACAD10_11_N-like cd05154
N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This ...
 66-253 1.43e-11

N-terminal domain of Acyl-CoA dehydrogenase (ACAD) 10 and 11, and similar proteins; This subfamily is composed of the N-terminal domains of vertebrate ACAD10 and ACAD11, and similar uncharacterized bacterial and eukaryotic proteins. ACADs are a family of flavoproteins that are involved in the beta-oxidation of fatty acyl-CoA derivatives. ACAD deficiency can cause metabolic disorders including muscle fatigue, hypoglycemia, and hepatic lipidosis. There are at least 11 distinct ACADs, some of which show distinct substrate specificities to either straight-chain or branched-chain fatty acids. ACAD10 is widely expressed in human tissues and highly expressed in liver, kidney, pancreas, and spleen. ACAD10 and ACAD11 are both significantly expressed in human brain tissues. They contain a long N-terminal domain with similarity to phosphotransferases with a Protein Kinase fold, which is absent in other ACADs. They may exhibit multiple functions in acyl-CoA oxidation pathways. ACAD11 utilizes substrates with carbon chain lengths of 20 to 26, with optimal activity towards C22CoA. ACAD10 may be associated with an increased risk in type II diabetes. The ACAD10/11-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270703 [Multi-domain]  Cd Length: 254  Bit Score: 62.63  E-value: 1.43e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  66 EAARLSWLATTGVPCAAVLDVVTEA---GRDWLLL----GEVPGQDLLSSHLAPAEKVSI---MADAMRRLHTLDPATCP 135
Cdd:cd05154   48 EYRVLRALAGTGVPVPRVLALCEDPsvlGAPFYVMervdGRVLPDPLPRPDLSPEERRALarsLVDALAALHSVDPAALG 127

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 136 FDHQAKHR------IERARTRMEAGLVDQDDLDEEhqglapaeLFARLKARMPDGEDLVVTHGDACLPNIMV-ENGRFSG 208
Cdd:cd05154  128 LADLGRPEgylerqVDRWRRQLEAAATDPPPALEE--------ALRWLRANLPADGRPVLVHGDFRLGNLLFdPDGRVTA 199

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 827356527 209 FIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPD 253
Cdd:cd05154  200 VLDWELATLGDPLEDLAWLLARWWRPGDPPGLAAPTRLPGFPSRE 244
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 36-233 8.54e-10

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 56.16  E-value: 8.54e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  36 DAAVFRLSAQGRPVLFVKTDLsgALNELQDEAARLSWLA-TTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSH--LAP 112
Cdd:cd05120   11 DNKVYLLGDPREYVLKIGPPR--LKKDLEKEAAMLQLLAgKLSLPVPKVYGFGESDGWEYLLMERIEGETLSEVWprLSE 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 113 AEKVSIM---ADAMRRLHTLDPatcpfdhqakhrierartrmeaglvdqddldeehqglapaelfarlkarmpdgedLVV 189
Cdd:cd05120   89 EEKEKIAdqlAEILAALHRIDS-------------------------------------------------------SVL 113

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 827356527 190 THGDACLPNIMVEN-GRFSGFIDCGRLGVADRYQDIALATRDIAE 233
Cdd:cd05120  114 THGDLHPGNILVKPdGKLSGIIDWEFAGYGPPAFDYAAALRDWTE 158
SrkA COG2334
Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal ...
 62-233 1.62e-08

Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist [Signal transduction mechanisms]; Ser/Thr protein kinase RdoA involved in Cpx stress response, MazF antagonist is part of the Pathway/BioSystem: Threonine biosynthesis


Pssm-ID: 441905 [Multi-domain]  Cd Length: 297  Bit Score: 54.16  E-value: 1.62e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  62 ELQDEAARLSWLATTGVPCAAVL------DVVTEAGRDWLLLGEVPGQDLLSSHLAPAEkvsIMADAMRRLHTldpATCP 135
Cdd:COG2334   53 EIPFELALLAHLAAAGLPVPAPVptrdgeTLLELEGRPAALFPFLPGRSPEEPSPEQLE---ELGRLLARLHR---ALAD 126

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 136 FDHQAKHRIErARTRMEAGLVDQDDLDEEHQGLAPAEL--FARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCG 213
Cdd:COG2334  127 FPRPNARDLA-WWDELLERLLGPLLPDPEDRALLEELLdrLEARLAPLLGALPRGVIHGDLHPDNVLFDGDGVSGLIDFD 205

                        170       180
                 ....*....|....*....|
gi 827356527 214 RLGVADRYQDIALATRDIAE 233
Cdd:COG2334  206 DAGYGPRLYDLAIALNGWAD 225
HomoserineK_II cd05153
Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a ...
 62-227 7.21e-07

Type II Homoserine Kinase; This subfamily is composed of unusual homoserine kinases, from a subset of bacteria, which have a Protein Kinase fold. These proteins do not bear any similarity to the GHMP family homoserine kinases present in most bacteria and eukaryotes. Homoserine kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to L-homoserine producing L-homoserine phosphate, an intermediate in the production of the amino acids threonine, methionine, and isoleucine. The Type II homoserine kinase subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270702 [Multi-domain]  Cd Length: 300  Bit Score: 49.18  E-value: 7.21e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  62 ELQDEAARLSWLATTGVPCAAVL------DVVTEAGRDWLLLGEVPGQDLLSSHlapAEKVSIMADAMRRLHTldpATCP 135
Cdd:cd05153   53 ELPFELELLDHLAQAGLPVPRPLadkdgeLLGELNGKPAALFPFLPGESLTTPT---PEQCRAIGAALARLHL---ALAG 126

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 136 FDHQAKHR---------IERARTRmeAGLVDQDDLDEEhqglapAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRF 206
Cdd:cd05153  127 FPPPRPNPrglawwkplAERLKAR--LDLLAADDRALL------EDELARLQALAPSDLPRGVIHADLFRDNVLFDGDRL 198

                        170       180
                 ....*....|....*....|.
gi 827356527 207 SGFIDCGRLGVADRYQDIALA 227
Cdd:cd05153  199 SGIIDFYDACYDPLLYDLAIA 219
APH_ChoK_like_1 cd05155
Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and ...
 120-219 1.05e-04

Uncharacterized bacterial proteins with similarity to Aminoglycoside 3'-phosphotransferase and Choline kinase; This subfamily is composed of uncharacterized bacterial proteins with similarity to APH and ChoK. Other APH/ChoK-like proteins include ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). These proteins catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates, such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides, and macrolides leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK-like subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270704 [Multi-domain]  Cd Length: 234  Bit Score: 42.61  E-value: 1.05e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 120 ADAMRRLHTLDPATCPFDHQAKH---RIERARTRMEAGLVDQDDLDEEhqglAPAELFARLKARMPDGEDLVVTHGDACL 196
Cdd:cd05155   97 ARFLAALHAIDPAGPPNPGRGNPlrgRDLAVRDAEEALAALAGLLDVA----AARALWERALAAPAWAGPPVWLHGDLHP 172

                         90       100
                 ....*....|....*....|...
gi 827356527 197 PNIMVENGRFSGFIDCGRLGVAD 219
Cdd:cd05155  173 GNLLVRDGRLSAVIDFGDLGVGD 195
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
125-266 1.79e-04

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 40.92  E-value: 1.79e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527 125 RLHTLdPATCPFDhqAKHRIERARtrmeaglvdqDDLDEEHQGLApaELFARLKARMP-DGEDLVVTHGDACLPNIMV-E 202
Cdd:COG0510    1 RLHAS-PALLRFD--LFARLERYL----------ALGPRDLPELL--RRLEELERALAaRPLPLVLCHGDLHPGNFLVtD 65

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 827356527 203 NGRFSgFIDCGRLGVADRYQDIALATRDIaeELGGEWADRFLVLYGIAAPDS---QRIAFYRLLDEF 266
Cdd:COG0510   66 DGRLY-LIDWEYAGLGDPAFDLAALLVEY--GLSPEQAEELLEAYGFGRPTEellRRLRAYRALADL 129
FN3K COG3001
Fructosamine-3-kinase [Carbohydrate transport and metabolism];
39-97 3.41e-04

Fructosamine-3-kinase [Carbohydrate transport and metabolism];


Pssm-ID: 442239 [Multi-domain]  Cd Length: 287  Bit Score: 41.34  E-value: 3.41e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  39 VFRLSAQGRPVlFVKTDLSGALNELQDEAARLSWLA-TTGVPCAAVLDVVTEAGRDWLLL 97
Cdd:COG3001   31 AYRVTTDGRRV-FVKLNPASPLGMFEAEAAGLRALAaTGTIRVPEVIGVGTTGDHAFLVL 89
MPH2' cd05152
Macrolide 2'-Phosphotransferase; MPH2' catalyzes the transfer of the gamma-phosphoryl group ...
 76-226 3.33e-03

Macrolide 2'-Phosphotransferase; MPH2' catalyzes the transfer of the gamma-phosphoryl group from ATP to the 2'-hydroxyl of macrolide antibiotics such as erythromycin, clarithromycin, and azithromycin, among others. Macrolides penetrate the bacterial cell and bind to ribosomes, where it interrupts protein elongation, leading ultimately to the demise of the bacterium. Phosphorylation of macrolides leads to their inactivation. Based on substrate specificity and amino acid sequence, MPH2' is divided into types I and II, encoded by mphA and mphB genes, respectively. MPH2'I inactivates 14-membered ring macrolides while MPH2'II inactivates both 14- and 16-membered ring macrolides. Enzymatic inactivation of macrolides has been reported as a mechanism for bacterial resistance in clinical samples. MPH2' is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270701 [Multi-domain]  Cd Length: 276  Bit Score: 37.99  E-value: 3.33e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 827356527  76 TGVPcAAVLDVVTEAgRDWLLLGEVPGQDLLSShlapaekvsiMADAMRRLHTLDPATCPFDHQAKHRIERARTRMeagl 155
Cdd:cd05152   87 PGVP-AATIDPEIQN-YVWNWDPLAPPPVFARS----------LGKALAALHSIPADLAAAAGLPVYTAEEVRARM---- 150

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 827356527 156 vdQDDLDE-EHQGLAPAELFARLKARMPDG----EDLVVTHGDACLPNIMV-ENGRFSGFIDCGRLGVADRYQDIAL 226
Cdd:cd05152  151 --AARMDRvKETFGVPPALLARWQAWLADDslwpFHTVLVHGDLHPGHILVdEDGRVTGLIDWTEAKVGDPADDFAW 225
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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