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Conserved domains on  [gi|944376342|gb|ALL72639|]
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DnaJ, partial [Aeromonas sp. MJ-61]

Protein Classification

molecular chaperone DnaJ( domain architecture ID 1000952)

molecular chaperone DnaJ, part of the DnaK-DnaJ-GrpE system, prevents the aggregation of unfolded substrate and acts primarily by stimulating the ATPase activity of Hsp70

CATH:  2.10.230.10
Gene Ontology:  GO:0051082|GO:0005524|GO:0009408|GO:0006457
PubMed:  8016869|15063739|29290615
SCOP:  4003813|4002149

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10767 super family cl35946
chaperone protein DnaJ; Provisional
 2-83 1.37e-55

chaperone protein DnaJ; Provisional


The actual alignment was detected with superfamily member PRK10767:

Pssm-ID: 236757 [Multi-domain]  Cd Length: 371  Bit Score: 174.95  E-value: 1.37e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK10767 135 KEIRIPTLVTCDTCHGSGAKPGTSPKTCPTCHGAGQVRMQQGFFTVQQTCPTCHGRGKIIKDPCKKCHGQGRVEKEKTLS 214


                 ..
gi 944376342  82 VK 83
Cdd:PRK10767 215 VK 216
 
Name Accession Description Interval E-value
PRK10767 PRK10767
chaperone protein DnaJ; Provisional
 2-83 1.37e-55

chaperone protein DnaJ; Provisional


Pssm-ID: 236757 [Multi-domain]  Cd Length: 371  Bit Score: 174.95  E-value: 1.37e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK10767 135 KEIRIPTLVTCDTCHGSGAKPGTSPKTCPTCHGAGQVRMQQGFFTVQQTCPTCHGRGKIIKDPCKKCHGQGRVEKEKTLS 214


                 ..
gi 944376342  82 VK 83
Cdd:PRK10767 215 VK 216
DnaJ_bact TIGR02349
chaperone protein DnaJ; This model represents bacterial forms of DnaJ, part of the ...
 1-83 1.26e-36

chaperone protein DnaJ; This model represents bacterial forms of DnaJ, part of the DnaK-DnaJ-GrpE chaperone system. The three components typically are encoded by consecutive genes. DnaJ homologs occur in many genomes, typically not near DnaK and GrpE-like genes; most such genes are not included by this family. Eukaryotic (mitochondrial and chloroplast) forms are not included in the scope of this family.


Pssm-ID: 274090 [Multi-domain]  Cd Length: 354  Bit Score: 125.41  E-value: 1.26e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342    1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQR 76
Cdd:TIGR02349 135 EKEIEIPRKESCETCHGTGAKPGTDPKTCPTCGGTGQVRRQQgtpfGFFQQQQTCPTCGGEGKIIKEPCSTCKGKGRVKE 214


                  ....*..
gi 944376342   77 TKTLSVK 83
Cdd:TIGR02349 215 RKTITVK 221
DnaJ_C pfam01556
DnaJ C terminal domain; This family consists of the C terminal region of the DnaJ protein. It ...
 1-83 2.60e-25

DnaJ C terminal domain; This family consists of the C terminal region of the DnaJ protein. It is always found associated with pfam00226 and pfam00684. DnaJ is a chaperone associated with the Hsp70 heat-shock system involved in protein folding and renaturation after stress. The two C-terminal domains CTDI and CTDII, both incorporated in this family are necessary for maintaining the J-domains in their specific relative positions. Structural analysis of PDB:1nlt shows that PF00684 is nested within this DnaJ C-terminal region.


Pssm-ID: 460251 [Multi-domain]  Cd Length: 213  Bit Score: 93.09  E-value: 2.60e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342    1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRyQRTKTL 80
Cdd:pfam01556  17 TKKIKITRNVICDTCGGSGAKPGTSPKTCPCCGGGGQVRRQFGFFSTCTCCPCCGGGGKIIDKCCKCCGGGGV-VEKKTL 95


                  ...
gi 944376342   81 SVK 83
Cdd:pfam01556  96 EVK 98
DnaJ_zf cd10719
Zinc finger domain of DnaJ and HSP40; Central/middle or CxxCxGxG-motif containing domain of ...
 12-72 5.50e-21

Zinc finger domain of DnaJ and HSP40; Central/middle or CxxCxGxG-motif containing domain of DnaJ/Hsp40 (heat shock protein 40). DnaJ proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, an important chaperonin family. Hsp40 proteins are characterized by the presence of an N-terminal J domain, which mediates the interaction with Hsp70. This central domain contains four repeats of a CxxCxGxG motif and binds to two Zinc ions. It has been implicated in substrate binding.


Pssm-ID: 199908 [Multi-domain]  Cd Length: 65  Bit Score: 78.07  E-value: 5.50e-21
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 944376342 12 CEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEG 72
Cdd:cd10719   1 CPTCNGSGAKPGTKPKTCPTCGGSGQVRQVQgtgfGFFQTQTTCPTCGGTGKIIKDPCPKCKGKG 65
 
Name Accession Description Interval E-value
PRK10767 PRK10767
chaperone protein DnaJ; Provisional
 2-83 1.37e-55

chaperone protein DnaJ; Provisional


Pssm-ID: 236757 [Multi-domain]  Cd Length: 371  Bit Score: 174.95  E-value: 1.37e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK10767 135 KEIRIPTLVTCDTCHGSGAKPGTSPKTCPTCHGAGQVRMQQGFFTVQQTCPTCHGRGKIIKDPCKKCHGQGRVEKEKTLS 214


                 ..
gi 944376342  82 VK 83
Cdd:PRK10767 215 VK 216
DnaJ_bact TIGR02349
chaperone protein DnaJ; This model represents bacterial forms of DnaJ, part of the ...
 1-83 1.26e-36

chaperone protein DnaJ; This model represents bacterial forms of DnaJ, part of the DnaK-DnaJ-GrpE chaperone system. The three components typically are encoded by consecutive genes. DnaJ homologs occur in many genomes, typically not near DnaK and GrpE-like genes; most such genes are not included by this family. Eukaryotic (mitochondrial and chloroplast) forms are not included in the scope of this family.


Pssm-ID: 274090 [Multi-domain]  Cd Length: 354  Bit Score: 125.41  E-value: 1.26e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342    1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQR 76
Cdd:TIGR02349 135 EKEIEIPRKESCETCHGTGAKPGTDPKTCPTCGGTGQVRRQQgtpfGFFQQQQTCPTCGGEGKIIKEPCSTCKGKGRVKE 214


                  ....*..
gi 944376342   77 TKTLSVK 83
Cdd:TIGR02349 215 RKTITVK 221
PRK14294 PRK14294
chaperone protein DnaJ; Provisional
 2-83 9.61e-31

chaperone protein DnaJ; Provisional


Pssm-ID: 237664 [Multi-domain]  Cd Length: 366  Bit Score: 110.24  E-value: 9.61e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK14294 137 KEIRIQKLETCEECHGSGCEPGTSPTTCPQCGGSGQVTQSQGFFSIRTTCPRCRGMGKVIVSPCKTCHGQGRVRVSKTVQ 216


                 ..
gi 944376342  82 VK 83
Cdd:PRK14294 217 VK 218
PRK14301 PRK14301
chaperone protein DnaJ; Provisional
 3-83 1.43e-28

chaperone protein DnaJ; Provisional


Pssm-ID: 237668 [Multi-domain]  Cd Length: 373  Bit Score: 104.82  E-value: 1.43e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   3 EIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLSV 82
Cdd:PRK14301 138 TLRIPKNVTCDDCGGSGAAPGTSPETCRHCGGSGQVRQSQGFFQIAVPCPVCRGEGRVITHPCPKCKGSGIVQQTRELKV 217


                 .
gi 944376342  83 K 83
Cdd:PRK14301 218 R 218
PRK14286 PRK14286
chaperone protein DnaJ; Provisional
 3-83 2.63e-28

chaperone protein DnaJ; Provisional


Pssm-ID: 172774 [Multi-domain]  Cd Length: 372  Bit Score: 103.92  E-value: 2.63e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   3 EIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLSV 82
Cdd:PRK14286 144 KIEIPRLESCVDCNGSGASKGSSPTTCPDCGGSGQIRRTQGFFSVATTCPTCRGKGTVISNPCKTCGGQGLQEKRRTINI 223


                 .
gi 944376342  83 K 83
Cdd:PRK14286 224 K 224
PRK14280 PRK14280
molecular chaperone DnaJ;
2-83 3.54e-28

molecular chaperone DnaJ;


Pssm-ID: 237656 [Multi-domain]  Cd Length: 376  Bit Score: 103.65  E-value: 3.54e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14280 136 KEIEIPKEETCDTCHGSGAKPGTSKETCSHCGGSGQVSVEQntpfGRVVNRQTCPHCNGTGQEIKEKCPTCHGKGKVRKR 215


                 ....*.
gi 944376342  78 KTLSVK 83
Cdd:PRK14280 216 KKINVK 221
PRK14277 PRK14277
chaperone protein DnaJ; Provisional
 2-82 6.93e-28

chaperone protein DnaJ; Provisional


Pssm-ID: 184599 [Multi-domain]  Cd Length: 386  Bit Score: 102.96  E-value: 6.93e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14277 148 KEIEVERFEKCDVCKGSGAKPGSKPVTCPVCHGTGQVRTRQntpfGRIVNIRTCDRCHGEGKIITDPCNKCGGTGRIRRR 227


                 ....*
gi 944376342  78 KTLSV 82
Cdd:PRK14277 228 RKIKV 232
PRK14293 PRK14293
molecular chaperone DnaJ;
2-82 2.37e-27

molecular chaperone DnaJ;


Pssm-ID: 237663 [Multi-domain]  Cd Length: 374  Bit Score: 101.61  E-value: 2.37e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVqmRQ------GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQ 75
Cdd:PRK14293 136 KEIRIPHLETCETCRGSGAKPGTGPTTCSTCGGAGQV--RRatrtpfGSFTQVSECPTCNGTGQVIEDPCDACGGQGVKQ 213


                 ....*..
gi 944376342  76 RTKTLSV 82
Cdd:PRK14293 214 VTKKLKI 220
PRK14283 PRK14283
chaperone protein DnaJ; Provisional
 1-83 3.85e-26

chaperone protein DnaJ; Provisional


Pssm-ID: 184604 [Multi-domain]  Cd Length: 378  Bit Score: 98.36  E-value: 3.85e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQV-QMRQ---GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQR 76
Cdd:PRK14283 138 EKDIKVRHTKKCPVCNGSRAEPGSEVKTCPTCGGTGQVkQVRNtilGQMMNVTTCPDCQGEGKIVEKPCSNCHGKGVVRE 217


                 ....*..
gi 944376342  77 TKTLSVK 83
Cdd:PRK14283 218 TKTISVK 224
PRK14300 PRK14300
chaperone protein DnaJ; Provisional
 2-82 6.46e-26

chaperone protein DnaJ; Provisional


Pssm-ID: 172788 [Multi-domain]  Cd Length: 372  Bit Score: 97.78  E-value: 6.46e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK14300 138 KNISFSSEVKCDTCHGSGSEKGETVTTCDACSGVGATRMQQGFFTIEQACHKCQGNGQIIKNPCKKCHGMGRYHKQRNLS 217


                 .
gi 944376342  82 V 82
Cdd:PRK14300 218 V 218
PRK14276 PRK14276
chaperone protein DnaJ; Provisional
 2-83 8.62e-26

chaperone protein DnaJ; Provisional


Pssm-ID: 237653 [Multi-domain]  Cd Length: 380  Bit Score: 97.47  E-value: 8.62e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14276 139 KEVSYNREATCHTCNGSGAKPGTSPVTCGKCHGSGVITVDTqtplGMMRRQVTCDVCHGTGKEIKEPCQTCHGTGHEKQA 218


                 ....*.
gi 944376342  78 KTLSVK 83
Cdd:PRK14276 219 HTVSVK 224
PRK14297 PRK14297
molecular chaperone DnaJ;
2-83 2.15e-25

molecular chaperone DnaJ;


Pssm-ID: 184611 [Multi-domain]  Cd Length: 380  Bit Score: 96.39  E-value: 2.15e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14297 141 KEISVTRNENCETCNGTGAKPGTSPKTCDKCGGTGQIRVQRntplGSFVSTTTCDKCGGSGKVIEDPCNKCHGKGKVRKN 220


                 ....*.
gi 944376342  78 KTLSVK 83
Cdd:PRK14297 221 RKIKVN 226
DnaJ_C pfam01556
DnaJ C terminal domain; This family consists of the C terminal region of the DnaJ protein. It ...
 1-83 2.60e-25

DnaJ C terminal domain; This family consists of the C terminal region of the DnaJ protein. It is always found associated with pfam00226 and pfam00684. DnaJ is a chaperone associated with the Hsp70 heat-shock system involved in protein folding and renaturation after stress. The two C-terminal domains CTDI and CTDII, both incorporated in this family are necessary for maintaining the J-domains in their specific relative positions. Structural analysis of PDB:1nlt shows that PF00684 is nested within this DnaJ C-terminal region.


Pssm-ID: 460251 [Multi-domain]  Cd Length: 213  Bit Score: 93.09  E-value: 2.60e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342    1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRyQRTKTL 80
Cdd:pfam01556  17 TKKIKITRNVICDTCGGSGAKPGTSPKTCPCCGGGGQVRRQFGFFSTCTCCPCCGGGGKIIDKCCKCCGGGGV-VEKKTL 95


                  ...
gi 944376342   81 SVK 83
Cdd:pfam01556  96 EVK 98
PRK14298 PRK14298
chaperone protein DnaJ; Provisional
 2-83 2.03e-23

chaperone protein DnaJ; Provisional


Pssm-ID: 184612 [Multi-domain]  Cd Length: 377  Bit Score: 91.06  E-value: 2.03e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQV-QMRQ---GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14298 134 KDIDVPRAERCSTCSGTGAKPGTSPKRCPTCGGTGQVtTTRStplGQFVTTTTCSTCHGRGQVIESPCPVCSGTGKVRKT 213


                 ....*.
gi 944376342  78 KTLSVK 83
Cdd:PRK14298 214 RKITVN 219
PRK14291 PRK14291
chaperone protein DnaJ; Provisional
 3-83 2.36e-23

chaperone protein DnaJ; Provisional


Pssm-ID: 237661 [Multi-domain]  Cd Length: 382  Bit Score: 90.98  E-value: 2.36e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   3 EIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGkIIKDPCRKCHGEGRYQRTKTLSV 82
Cdd:PRK14291 150 SLEVPRYVPCEACGGTGYDPGSGEKVCPTCGGSGEIYQRGGFFRISQTCPTCGGEG-VLREPCSKCNGRGLVIKKETIKV 228


                 .
gi 944376342  83 K 83
Cdd:PRK14291 229 R 229
PRK14278 PRK14278
chaperone protein DnaJ; Provisional
 1-83 2.42e-23

chaperone protein DnaJ; Provisional


Pssm-ID: 237654 [Multi-domain]  Cd Length: 378  Bit Score: 90.88  E-value: 2.42e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQ----QACPHCHGRGKIIKDPCRKCHGEGRYQR 76
Cdd:PRK14278 131 TKQVTVDTAVLCDRCHGKGTAGDSKPVTCDTCGGRGEVQTVQRSFLGQvmtsRPCPTCRGVGEVIPDPCHECAGDGRVRA 210


                 ....*..
gi 944376342  77 TKTLSVK 83
Cdd:PRK14278 211 RREITVK 217
PRK14281 PRK14281
chaperone protein DnaJ; Provisional
 2-82 5.19e-23

chaperone protein DnaJ; Provisional


Pssm-ID: 237657 [Multi-domain]  Cd Length: 397  Bit Score: 89.87  E-value: 5.19e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSaQTCPTCHGSGQVqmRQ------GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQ 75
Cdd:PRK14281 156 KTLKIKKQVPCKECNGTGSKTGAT-ETCPTCHGSGEV--RQasktmfGQFVNITACPTCGGEGRVVKDRCPACYGEGIKQ 232


                 ....*..
gi 944376342  76 RTKTLSV 82
Cdd:PRK14281 233 GEVTVKV 239
PRK14284 PRK14284
chaperone protein DnaJ; Provisional
 2-82 6.07e-23

chaperone protein DnaJ; Provisional


Pssm-ID: 237658 [Multi-domain]  Cd Length: 391  Bit Score: 89.90  E-value: 6.07e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLS 81
Cdd:PRK14284 151 KELLVSGYKSCDACSGSGANSSQGIKVCDRCKGSGQVVQSRGFFSMASTCPECGGEGRVITDPCSVCRGQGRIKDKRSVH 230


                 .
gi 944376342  82 V 82
Cdd:PRK14284 231 V 231
DnaJ_CXXCXGXG pfam00684
DnaJ central domain; The central cysteine-rich (CR) domain of DnaJ proteins contains four ...
 12-72 1.19e-22

DnaJ central domain; The central cysteine-rich (CR) domain of DnaJ proteins contains four repeats of the motif CXXCXGXG where X is any amino acid. The isolated cysteine rich domain folds in zinc dependent fashion. Each set of two repeats binds one unit of zinc. Although this domain has been implicated in substrate binding, no evidence of specific interaction between the isolated DNAJ cysteine rich domain and various hydrophobic peptides has been found.


Pssm-ID: 459904 [Multi-domain]  Cd Length: 65  Bit Score: 82.22  E-value: 1.19e-22
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 944376342  12 CEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEG 72
Cdd:pfam00684  1 CPTCNGSGAKPGTKPTTCPTCGGTGQVRRVQqtgpGFFQMQSTCPTCGGTGKIIKDPCKKCKGKG 65
PRK14279 PRK14279
molecular chaperone DnaJ;
12-83 2.67e-22

molecular chaperone DnaJ;


Pssm-ID: 237655 [Multi-domain]  Cd Length: 392  Bit Score: 87.87  E-value: 2.67e-22
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 944376342  12 CEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLSVK 83
Cdd:PRK14279 176 CTTCHGSGARPGTSPKVCPTCNGSGVISRNQGAFGFSEPCTDCRGTGSIIEDPCEECKGTGVTTRTRTINVR 247
PRK14289 PRK14289
molecular chaperone DnaJ;
2-72 7.61e-22

molecular chaperone DnaJ;


Pssm-ID: 237660 [Multi-domain]  Cd Length: 386  Bit Score: 86.81  E-value: 7.61e-22
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEG 72
Cdd:PRK14289 147 KKFKVKKYVPCSHCHGTGAEGNNGSETCPTCKGSGSVTRVQntilGTMQTQSTCPTCNGEGKIIKKKCKKCGGEG 221
PRK14295 PRK14295
molecular chaperone DnaJ;
1-83 8.75e-22

molecular chaperone DnaJ;


Pssm-ID: 237665 [Multi-domain]  Cd Length: 389  Bit Score: 86.44  E-value: 8.75e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   1 SKEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTL 80
Cdd:PRK14295 158 TVPLRLTSQAPCPACSGTGAKNGTTPRVCPTCSGTGQVSRNSGGFSLSEPCPDCKGRGLIADDPCLVCKGSGRAKSSRTM 237


                 ...
gi 944376342  81 SVK 83
Cdd:PRK14295 238 QVR 240
DnaJ_zf cd10719
Zinc finger domain of DnaJ and HSP40; Central/middle or CxxCxGxG-motif containing domain of ...
 12-72 5.50e-21

Zinc finger domain of DnaJ and HSP40; Central/middle or CxxCxGxG-motif containing domain of DnaJ/Hsp40 (heat shock protein 40). DnaJ proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, an important chaperonin family. Hsp40 proteins are characterized by the presence of an N-terminal J domain, which mediates the interaction with Hsp70. This central domain contains four repeats of a CxxCxGxG motif and binds to two Zinc ions. It has been implicated in substrate binding.


Pssm-ID: 199908 [Multi-domain]  Cd Length: 65  Bit Score: 78.07  E-value: 5.50e-21
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 944376342 12 CEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEG 72
Cdd:cd10719   1 CPTCNGSGAKPGTKPKTCPTCGGSGQVRQVQgtgfGFFQTQTTCPTCGGTGKIIKDPCPKCKGKG 65
PRK14296 PRK14296
chaperone protein DnaJ; Provisional
 2-82 9.05e-20

chaperone protein DnaJ; Provisional


Pssm-ID: 237666 [Multi-domain]  Cd Length: 372  Bit Score: 81.15  E-value: 9.05e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQV--QMRQGFFAVQQA--CPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14296 142 KIIELDLLTNCSKCFGSGAESNSDIHICNNCHGTGEVlvQKNMGFFQFQQSakCNVCNGAGKIIKNKCKNCKGKGKYLER 221


                 ....*
gi 944376342  78 KTLSV 82
Cdd:PRK14296 222 KKIEV 226
PRK14292 PRK14292
chaperone protein DnaJ; Provisional
 3-83 2.30e-19

chaperone protein DnaJ; Provisional


Pssm-ID: 237662 [Multi-domain]  Cd Length: 371  Bit Score: 79.93  E-value: 2.30e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   3 EIKVPTLVHCEVCNGSGAHTGSSA-QTCPTCHGSGQVQMR-QGFFAV---QQACPHCHGRGKIIKDPCRKCHGEGRYQRT 77
Cdd:PRK14292 133 EVEVDRLTECEHCHGSRTEPGGKPpKTCPTCRGAGAVRAQaRTIFGVvetQQPCPTCRGEGQIITDPCTVCRGRGRTLKA 212


                 ....*.
gi 944376342  78 KTLSVK 83
Cdd:PRK14292 213 ETVKVK 218
PRK14285 PRK14285
chaperone protein DnaJ; Provisional
 12-83 9.49e-19

chaperone protein DnaJ; Provisional


Pssm-ID: 172773 [Multi-domain]  Cd Length: 365  Bit Score: 78.11  E-value: 9.49e-19
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 944376342  12 CEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLSVK 83
Cdd:PRK14285 149 CESCLGKKSEKGTSPSICNMCNGSGRVMQGGGFFRVTTTCPKCYGNGKIISNPCKSCKGKGSLKKKETIELK 220
PRK14290 PRK14290
chaperone protein DnaJ; Provisional
 1-83 2.28e-18

chaperone protein DnaJ; Provisional


Pssm-ID: 172778 [Multi-domain]  Cd Length: 365  Bit Score: 76.89  E-value: 2.28e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   1 SKEIKVPTLVHCEVCNGSGAHTGSSAqTCPTCHGSGQ--VQMRQGFF--AVQQACPHCHGRGKIIKDPCRKCHGEGRYQR 76
Cdd:PRK14290 141 EKRIKYRRNAMCPDCSGTGAKNGKLI-TCPTCHGTGQqrIVRGQGFFrmVTVTTCRTCGGRGRIPEEKCPRCNGTGTVVV 219


                 ....*..
gi 944376342  77 TKTLSVK 83
Cdd:PRK14290 220 NEDISVK 226
PRK14287 PRK14287
chaperone protein DnaJ; Provisional
 3-83 8.63e-18

chaperone protein DnaJ; Provisional


Pssm-ID: 237659 [Multi-domain]  Cd Length: 371  Bit Score: 75.43  E-value: 8.63e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   3 EIKVPTLVHCEVCNGSGAHTGSSAQTCPTCHGSGQVQMRQ----GFFAVQQACPHCHGRGKIIKDPCRKCHGEGRYQRTK 78
Cdd:PRK14287 132 EIEIPREETCGTCHGSGAKPGTKPETCSHCGGSGQLNVEQntpfGRVVNRRVCHHCEGTGKIIKQKCATCGGKGKVRKRK 211


                 ....*
gi 944376342  79 TLSVK 83
Cdd:PRK14287 212 KINVK 216
PRK14288 PRK14288
molecular chaperone DnaJ;
2-71 5.75e-17

molecular chaperone DnaJ;


Pssm-ID: 172776 [Multi-domain]  Cd Length: 369  Bit Score: 73.18  E-value: 5.75e-17
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 944376342   2 KEIKVPTLVHCEVCNGSGAHTgSSAQTCPTCHGSGQVQMRQGFFAVQQACPHCHGRGKIIKDPCRKCHGE 71
Cdd:PRK14288 133 KTIKVQYQSVCESCDGTGAKD-KALETCKQCNGQGQVFMRQGFMSFAQTCGACQGKGKIIKTPCQACKGK 201
PRK14282 PRK14282
chaperone protein DnaJ; Provisional
 12-83 9.25e-17

chaperone protein DnaJ; Provisional


Pssm-ID: 184603 [Multi-domain]  Cd Length: 369  Bit Score: 72.52  E-value: 9.25e-17
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 944376342  12 CEVCNGSGAHTGSSAQTCPTCHGSGQV-QMRQGFFAV---QQACPHCHGRGKIIKDPCRKCHGEGRYQRTKTLSVK 83
Cdd:PRK14282 155 CPHCGGTGVEPGSGYVTCPKCHGTGRIrEERRSFFGVfvsERTCERCGGTGKIPGEYCHECGGSGRIRRRVRTTVK 230
PTZ00037 PTZ00037
DnaJ_C chaperone protein; Provisional
 12-82 1.12e-09

DnaJ_C chaperone protein; Provisional


Pssm-ID: 240236 [Multi-domain]  Cd Length: 421  Bit Score: 52.90  E-value: 1.12e-09
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 944376342  12 CEVCNGSGAHTgSSAQTCPTCHGSG-QVQMRQGFFAVQQA---CPHCHGRGKII--KDPCRKCHGEGRYQRTKTLSV 82
Cdd:PTZ00037 153 CANCEGHGGPK-DAFVDCKLCNGQGiRVQIRQMGSMIHQTqstCNSCNGQGKIIpeSKKCKNCSGKGVKKTRKILEV 228
phage_xxxx TIGR02642
uncharacterized phage protein; This uncharacterized protein is found in prophage regions of ...
 27-62 1.83e-04

uncharacterized phage protein; This uncharacterized protein is found in prophage regions of Shewanella oneidensis MR-1, Vibrio vulnificus YJ016, Yersinia pseudotuberculosis IP 32953, and Aeromonas hydrophila ATCC7966. It appears to have regions of sequence similarity to phage lambda antitermination protein Q. [Mobile and extrachromosomal element functions, Prophage functions]


Pssm-ID: 274243 [Multi-domain]  Cd Length: 186  Bit Score: 37.55  E-value: 1.83e-04
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 944376342   27 QTCPTCHGSGQVQMRQGffavqqACPHCHGRGKIIK 62
Cdd:TIGR02642 100 CKCPRCRGTGLIQRRQR------ECDTCAGTGRFRP 129
anti-TRAP cd10748
anti-TRAP (AT) protein specific to Bacilli; In Bacillus subtilis and related bacteria, AT ...
 47-72 8.20e-03

anti-TRAP (AT) protein specific to Bacilli; In Bacillus subtilis and related bacteria, AT binds to the TRAP protein, (tryptophan-activated trp RNA-binding attenuation protein), effectively disrupting interaction of TRAP with mRNAs. Upon binding of tryptophan, TRAP (which forms a complex of 11 identical subunits) interacts with a specific location in the leader RNA and blocks translation of the tryptophan biosynthetic operon. AT, in turn, recognizes the tryptophan-activated TRAP complex and prevents RNA binding. AT is expressed in response to high levels of uncharged tryptophan tRNA. AT contains a zinc-binding motif that closely resembles the zinc-binding motifs in the zinc-finger region of DnaJ/Hsp40. AT has been shown to form homo-dodecameric assemblies, and can actually do that in two different relative orientations, resulting in two different dodecamers. Recent data suggest that the trimeric form of AT may be the biologically relevant active complex.


Pssm-ID: 199910 [Multi-domain]  Cd Length: 52  Bit Score: 31.44  E-value: 8.20e-03
                        10        20
                ....*....|....*....|....*.
gi 944376342 47 VQQACPHCHGRGKIIKDPCRKCHGEG 72
Cdd:cd10748   7 LEVTCPKCERAGEEEGTPCPKCSGKG 32
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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