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Conserved domains on  [gi|1266971053|gb|ATM34467|]
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beta-glucanase [Klebsiella pneumoniae]

Protein Classification

glycoside hydrolase family 43 protein( domain architecture ID 13039884)

glycoside hydrolase family 43 (GH43) protein such as alpha-L-arabinofuranosidase and beta-D-xylosidase, which hydrolyze monosaccharide residues from the non-reducing termini of substrates

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH43_CtGH43-like cd18825
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 49-358 3.65e-123

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


:

Pssm-ID: 350146 [Multi-domain]  Cd Length: 285  Bit Score: 356.52  E-value: 3.65e-123
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIYYDGEYYYFYGEYRVGksifSNEWQGSNQKVNMYRSKDLVHWMPVGIVLDL------SHLPENYQLERPKII 122
Cdd:cd18825     1 IQAHGGGILKHNGTYYWYGEDKTG----GTYRRVDVIGVSCYSSKDLYNWKDEGIVLDAvddapaSDLYPNNVVERPKVI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 123 YNKKNKEYVMWFHLEkNKKFTDGSGEAGVATSNNITGPYTFISASYSNAGVKpfspaantdssnwfvrqankrfEKYFSR 202
Cdd:cd18825    77 YNKKTKKYVMWFHLD-GPGADYSRARAGVAVSDSPTGPFKYLGSFRPNAGEK----------------------NRDFSN 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 203 GQPVRDMALFVDDDEKAYLIYESEDNYSLQVARLSDDYKSFSGVYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNA 282
Cdd:cd18825   134 GQMSRDMTLFVDDDGKAYLIYSSEENKTLYIAKLTDDYTGVTGDYARILIGQSREAPAVFKHDGKYYMITSGCTGWAPNA 213

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1266971053 283 ARISVADNILGPWVTLGNPMSSfNSNDILTSFHSQPTYILKVKS--DKYIYLGDRWDfknnsYSDLWKSTYVWLPINF 358
Cdd:cd18825   214 ARYAVADSIFGPWKEIGNPCRG-PNDDADTTFGSQSTFVLPVDGenGKFIYMGDRWN-----PKDLADSRYVWLPITF 285
 
Name Accession Description Interval E-value
GH43_CtGH43-like cd18825
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 49-358 3.65e-123

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350146 [Multi-domain]  Cd Length: 285  Bit Score: 356.52  E-value: 3.65e-123
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIYYDGEYYYFYGEYRVGksifSNEWQGSNQKVNMYRSKDLVHWMPVGIVLDL------SHLPENYQLERPKII 122
Cdd:cd18825     1 IQAHGGGILKHNGTYYWYGEDKTG----GTYRRVDVIGVSCYSSKDLYNWKDEGIVLDAvddapaSDLYPNNVVERPKVI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 123 YNKKNKEYVMWFHLEkNKKFTDGSGEAGVATSNNITGPYTFISASYSNAGVKpfspaantdssnwfvrqankrfEKYFSR 202
Cdd:cd18825    77 YNKKTKKYVMWFHLD-GPGADYSRARAGVAVSDSPTGPFKYLGSFRPNAGEK----------------------NRDFSN 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 203 GQPVRDMALFVDDDEKAYLIYESEDNYSLQVARLSDDYKSFSGVYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNA 282
Cdd:cd18825   134 GQMSRDMTLFVDDDGKAYLIYSSEENKTLYIAKLTDDYTGVTGDYARILIGQSREAPAVFKHDGKYYMITSGCTGWAPNA 213

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1266971053 283 ARISVADNILGPWVTLGNPMSSfNSNDILTSFHSQPTYILKVKS--DKYIYLGDRWDfknnsYSDLWKSTYVWLPINF 358
Cdd:cd18825   214 ARYAVADSIFGPWKEIGNPCRG-PNDDADTTFGSQSTFVLPVDGenGKFIYMGDRWN-----PKDLADSRYVWLPITF 285
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
89-315 4.54e-21

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 93.09  E-value: 4.54e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVLD-LSHLPENYQ--LERPKIIYnkKNKEYVMWFhleKNKKFTDGSGEAGVATSNNITGPYTFIS 165
Cdd:COG3507    58 IFHSKDLVNWELVGHALDrLPQWADPYSggIWAPDIRY--HNGKYYLYY---TAVDGGKNRSGIGVATADDPEGPWSDPG 132

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 166 AsysnagvkpfspaantdssnwfvrqankrfekYFSRGQPVRDMALFVDDDEKAYLIYESEDNYsLQVARLSDDYKSFSG 245
Cdd:COG3507   133 P--------------------------------LVCPGGNGIDPSVFVDDDGKAYLVYGSGGGG-IYVAELDPDTGKLLG 179

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1266971053 246 VYSRIL---VGQLNEAPSIMKYKNKYYLITS-GLSGYRPNAARISVADNILGPWVTL-GNPMSSFNSNDILTSFH 315
Cdd:COG3507   180 EPKTLApggEGGWIEGPHIYKRNGYYYLFYSeGGTCNSGYAVRVARSKSPTGPYEDApGNPILTQRSDGGIQGPG 254
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
 77-309 2.43e-12

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 66.57  E-value: 2.43e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  77 SNEWQGsnqKVNMYRSKDLVHWMPVGIVLD-LSHLPENYQLER--PKIIYnkKNKEYVMWFhleknkkfTDGSGEAGVAT 153
Cdd:pfam04616  28 SFEWFP---GIPIFHSKDLVNWKLVGPVLVrRSQLSGRGSNASwaPDISY--HDGKYYLYY--------TAVAHGIFVAT 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 154 SNNITGPYTfisasysnagvKPFSPAANTDSSnwfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYESED----NY 229
Cdd:pfam04616  95 ADSPDGPWS-----------DPGKLKSGGGGI----------------------DPSLFHDDDGKKYLVWGGWDprhgHG 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 230 SLQVARLSDDYKSFSGVYSRILV-------GQLNEAPSIMKYKNKYYLITS-GLSGYrPNAARISVADNILGPWV-TLGN 300
Cdd:pfam04616 142 GIYLQELDNDGLKLVGPVTKLIYpgtrwvgGKVTEGPHLYKRNGYYYLTYAaGGTGG-PYAVGVARSRSPLGPYEwHPGN 220


                  ....*....
gi 1266971053 301 PMSSFNSND 309
Cdd:pfam04616 221 PILTSRSPE 229
 
Name Accession Description Interval E-value
GH43_CtGH43-like cd18825
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 49-358 3.65e-123

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350146 [Multi-domain]  Cd Length: 285  Bit Score: 356.52  E-value: 3.65e-123
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIYYDGEYYYFYGEYRVGksifSNEWQGSNQKVNMYRSKDLVHWMPVGIVLDL------SHLPENYQLERPKII 122
Cdd:cd18825     1 IQAHGGGILKHNGTYYWYGEDKTG----GTYRRVDVIGVSCYSSKDLYNWKDEGIVLDAvddapaSDLYPNNVVERPKVI 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 123 YNKKNKEYVMWFHLEkNKKFTDGSGEAGVATSNNITGPYTFISASYSNAGVKpfspaantdssnwfvrqankrfEKYFSR 202
Cdd:cd18825    77 YNKKTKKYVMWFHLD-GPGADYSRARAGVAVSDSPTGPFKYLGSFRPNAGEK----------------------NRDFSN 133

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 203 GQPVRDMALFVDDDEKAYLIYESEDNYSLQVARLSDDYKSFSGVYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNA 282
Cdd:cd18825   134 GQMSRDMTLFVDDDGKAYLIYSSEENKTLYIAKLTDDYTGVTGDYARILIGQSREAPAVFKHDGKYYMITSGCTGWAPNA 213

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1266971053 283 ARISVADNILGPWVTLGNPMSSfNSNDILTSFHSQPTYILKVKS--DKYIYLGDRWDfknnsYSDLWKSTYVWLPINF 358
Cdd:cd18825   214 ARYAVADSIFGPWKEIGNPCRG-PNDDADTTFGSQSTFVLPVDGenGKFIYMGDRWN-----PKDLADSRYVWLPITF 285
GH43_CtGH43-like cd08985
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
 49-358 1.28e-77

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350099 [Multi-domain]  Cd Length: 273  Bit Score: 240.31  E-value: 1.28e-77
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIYydgeyyyfygeyRVGKSIFsneWQGSNQK----------VNMYRSKDLVHWMPVGIVLDLSH------LPE 112
Cdd:cd08985     1 IHAHGGGIL------------QEGDTYY---WYGESRKgldndnlshgINCYSSTDLYNWRFEGLVLPASGvevvrdISP 65

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 113 NYQLERPKIIYNKKNKEYVMWFHLEKNKkftDGSGEAGVATSNNITGPYTFISASYSNagvkpfspaantdssnwfvrqa 192
Cdd:cd08985    66 GYVIERPKVLYNARTRKYVMWFHLDNPN---YGFAAVGVATSDTPTGPFTFVRSFRPD---------------------- 120

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 193 nkrfekyfsrGQPVRDMALFVDDDEKAYLIYESEDNYSLQVARLSDDYKSFSGVYSrILVGQLNEAPSIMKYKNKYYLIT 272
Cdd:cd08985   121 ----------GYPSRDMTLFQDPDGTAYLVRSTDHNTDIGISRLSDDYLDTTGASS-TFKGPKREAPALFKRGGTYYLIT 189

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 273 SGLSGYRPNAARISVADNILGPWVTLGNPMSSFNSNDilTSFHSQPTYILKVKS---DKYIYLGDRWDFKNNSYSDlwkS 349
Cdd:cd08985   190 SGLTGWNPNPSRLARADSPLGPWSTWGNLPVGGPGAD--TTYDSQPAFVFPVEGqggELFIYMGDRWNPGGGGVGN---A 264


                  ....*....
gi 1266971053 350 TYVWLPINF 358
Cdd:cd08985   265 TYVWLPLLF 273
GH43_Pc3Gal43A-like cd18821
Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1, ...
 49-358 2.02e-69

Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1,3-galactanase (Pc1, 3Gal43A, 1,3Gal43A); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), Fusarium oxysporum 12S Fo/1 (3Gal), and Streptomyces sp. 19(2012) SGalase1 and SGalase2. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350142 [Multi-domain]  Cd Length: 262  Bit Score: 218.64  E-value: 2.02e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIYydgeyyyfygeyRVGKSIF-----SNEWQGSNQKVNMYRSKDLVHWMPVGIVLDL---SHLPENYQLERPK 120
Cdd:cd18821     1 IQAHGGGIL------------KVGDTYYwfgedKTDGSNLFQGVSCYSSTDLVNWTFEGLALPPqesGDLGPNRVVERPK 68

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 121 IIYNKKNKEYVMWFHLEknkkfTDGSGEA--GVATSNNITGPYTFisasysnagVKPFSPaantdssnwfvrqankrfek 198
Cdd:cd18821    69 VIYNPSTGKYVMWMHID-----SSNYGDArvGVATSDTVTGPYTY---------VGSFRP-------------------- 114

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 199 yfsRGQPVRDMALFVDDDEKAYLIYESEDNySLQVARLSDDYKSFSGVYSRILVGQLnEAPSIMKYKNKYYLITSGLSGY 278
Cdd:cd18821   115 ---LGYESRDIGVFQDDDGTAYLLFEDRDN-GLRIYRLSDDYLSVVELVYTFIAAGL-EAPAMFKVDGTYYLLGSHLTGW 189

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 279 RPNAARISVADNILGPWVTLGN--PMSSfnsndiLTsFHSQPTYILKV---KSDKYIYLGDRWDfknnsYSDLWKSTYVW 353
Cdd:cd18821   190 RPNDNVYFTATSLSGPWSEPGLiaPEGT------NT-YNSQSTFVLPVggsKKTTYIYMGDRWD-----SPDLSASTYVW 257


                  ....*
gi 1266971053 354 LPINF 358
Cdd:cd18821   258 LPLTI 262
GH43_CtGH43-like cd18822
Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase ...
 82-358 9.97e-68

Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43), Streptomyces avermitilis MA-4680 = NBRC 14893 (Sa1,3Gal43A;SAV2109) (1,3Gal43A), and Ruminiclostridium thermocellum ATCC 27405 (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A). It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350143  Cd Length: 266  Bit Score: 214.41  E-value: 9.97e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  82 GSNQKVNMYRSKDLVHWMPVGIVLDLSHL--PENYQ--LERPKIIYNKKNKEYVMWFHLEKNKKFTDgsGEAGVATSNNI 157
Cdd:cd18822    26 NGFNGVSLYSSTDLVNWEFRNTVLTRDTCsaSELASckIERPKVIYNPKTGKFVMWAHWENGKDYGL--ARAAVATSDTP 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 158 TGPYTFISAsysnagvkpFSPAANtDSsnwfvrqankrfekyfsrgqpvRDMALFVDDDEKAYLIYESEDNYSLQVARLS 237
Cdd:cd18822   104 DGDYTFHGS---------FRPLGY-DS----------------------RDMTLFVDDDGTAYLISAANDNADLNIYRLT 151

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 238 DDYKSFSGVYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNAARISVADNILGPW---VTLGNPmssfnsndilTSF 314
Cdd:cd18822   152 PDYLSVDSLVATLFKGQHREAPALVKRNGYYYLFTSGASGWYPNQGQYASATSLAGPWsslRNIGNN----------TTF 221

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*..
gi 1266971053 315 HSQPTYILKV---KSDKYIYLGDRWdfKNNSYSDLWKSTYVWLPINF 358
Cdd:cd18822   222 GSQSTFILPVggsGGTSYLYMGDRW--NSPWGGDLGDSRYVWLPLSF 266
GH43_CtGH43-like cd18826
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 49-358 3.46e-64

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350147 [Multi-domain]  Cd Length: 269  Bit Score: 205.56  E-value: 3.46e-64
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  49 INAHGGGIyydgeyyyfygeyrvgksIFSNE---WQGSNQK------------VNMYRSKDLVHWMPVGIVLDL------ 107
Cdd:cd18826     1 IQAHGGSV------------------IYVDGvyyWYGENKEhtdgesgiwhwgVRCYSSTDLYNWEDEGLIIPPdpddps 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 108 SHLPENYQLERPKIIYNKKNKEYVMWFHLEKNKKFtdgsGEAGVATSNNITGPYTfisasYSNAGVKPFspaantdssnw 187
Cdd:cd18826    63 SPLHPTRIMDRPHIIYNEKTGKYVCWLKLYPGGDV----QYFGVLTADSPTGPYT-----YVHKFLGPL----------- 122

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 188 fvrqankrfekyfsrGQPVRDMALFVDDDEKAYLIYEsEDNYSLQVARLSDDYKSFSGVYSRILVGQL----NEAPSIMK 263
Cdd:cd18826   123 ---------------GMGAGDFDLVVDPDGKAYLYFE-RVHKEVVCADLTDDYTDVTGEYSTHFPGLGppfaREAPAVFK 186

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 264 YKNKYYLITSGLSGYRPNAARISVADNILGPWVTLGNPMSSFNSNdilTSFHSQPTYILKV--KSDKYIYLGDRwdfknn 341
Cdd:cd18826   187 RGGKHYLLTSGTTGYFPNPSEVAVADSYHGPWTVLGNPHVGDGSE---TSFNSQISSVFKVpgKKDLYIAMADR------ 257

                         330
                  ....*....|....*..
gi 1266971053 342 sysdlWKSTYVWLPINF 358
Cdd:cd18826   258 -----WISRYVWLPIRF 269
GH43_CtGH43-like cd18824
Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1, ...
 90-358 3.26e-32

Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A; This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350145 [Multi-domain]  Cd Length: 282  Bit Score: 122.14  E-value: 3.26e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  90 YRSKDLVHWMPVGIVLDLSHLPENYQLE-RPKIIYNKKNKEYVMWFHleknkkFTDGSGEAGVATSNNITGPYTFISAsy 168
Cdd:cd18824    40 YSSVDLVNWTYRGVLFDPNTCAGSPGVCfRPHVVYNARTGRYVLWYN------AYDGSSGYAVATSSTPTGPFVTVPD-- 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 169 snagvkpfsPAAntdssnwfvrqankrfekYFSRGQPVrDMALFVDDDEKAYLIYESED-NYSLQVARLSDDYKSFSGVY 247
Cdd:cd18824   112 ---------PVL------------------APAGLQAG-DFSLFVDDDGTGYLAYTTIDfPQSIVVEQLTDDYLNTTGEY 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 248 SRILVGQLNEAPSIMKYKNKYYLITS-GLSGYRPNA-ARISVADNILGPWVTLG----NPMSSFNSNDILTSFHSQPTYI 321
Cdd:cd18824   164 VRDLIDQEAEAPSIFKRNGIYYILASnTCCGCCQGTgARVYRATSPLGPWTRQIdinsCAGALFPPSDSAYTCGGQPTAV 243

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....
gi 1266971053 322 LKVKS----DKYIYLGDRWD---FKNNSysdlwKSTYVWLPINF 358
Cdd:cd18824   244 LPLPSpggeTLYLYMGDRWRtapDGRKG-----HDGHYWQPLSF 282
GH43_RcAra43A-like cd18823
Glycosyl hydrolase family 43 such as Ruminococcus champanellensis arabinanase Ara43A; This ...
 82-358 1.04e-31

Glycosyl hydrolase family 43 such as Ruminococcus champanellensis arabinanase Ara43A; This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis arabinanase Ara43A and Fibrobacter succinogenes subsp. succinogenes S85 Fisuc_1994 / FSU_2517. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350144 [Multi-domain]  Cd Length: 289  Bit Score: 121.30  E-value: 1.04e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  82 GSNQKVNMYRSKDLVHWMPVGIVLDLSH-------LPENYQLERPKIIYNKKNKEYVMWFHLEknkkfTDGSGEAGV--A 152
Cdd:cd18823    38 TSFKSVTLYSSTDLVNWTFEGNVLTASGavdtagdFAGAGWVGRPGVAYNSATGKYVLLIQWG-----STGNGRNGVlfA 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 153 TSNNITGPYTFISASYSNAGVkpfsPAANTDssnwfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYES-EDNYSL 231
Cdd:cd18823   113 TSDSPTGPFTYQRVQPMIDNV----GTNNTG------------------------DQTSFFDDDGKAYLVYSNdRGRGSL 164

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 232 QVARLSDDYKSF-SGVYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNAARISVADNILGPW-----VTLGNPMSSF 305
Cdd:cd18823   165 YIAKLRSDYLGIePAVRIDNYVGPGREGNALFKYGGTYYLCASDLHGWNASQTYYMVATSLTGPYspsnvLETTGPESDN 244

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1266971053 306 nsndiltSFHSQPTYILKVKSDK---YIYLGDRW-DFKNN--SYSDlwkstyvWLPINF 358
Cdd:cd18823   245 -------SHVTQTGFFIPVHGSKgttYVYCGDRWsDFAGNgiGYNQ-------WYPLTF 289
GH_F cd08978
Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F ...
 77-336 8.87e-26

Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F; This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350092 [Multi-domain]  Cd Length: 251  Bit Score: 104.06  E-value: 8.87e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  77 SNEWQGSNqKVNMYRSKDLVHWMPVGIVLDLSHLPE--NYQLERPKIIYNKKNKeYVMWFHLEKNKKftdgSGEAGVATS 154
Cdd:cd08978    18 TDDTGTGT-GIVVWKSKDLVNWKEEGTVLSRGKSKSwgTGNLWAPEVYYFNSGK-WYLYYSAVPNGG----GGRIYVATS 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 155 NNITGPYTFISasysnaGVKPFSPAANTdssnwfvrqankrfekyfsrgqpvRDMALFVDDDEKAYLIYESED-NYSLQV 233
Cdd:cd08978    92 DSPEGPFTPIV------SGKLGDRGSGS------------------------IDPTVFVDDDGKLYLYYGDEDdSGDIYV 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 234 ARLSDDYKSFSG--------VYSRILVGQLNEAPSIMKYKNKYYLITSGLSGYRPNAARISVADNILGPWVTLGNPMSSF 305
Cdd:cd08978   142 AELDPDLLTIKGdvtlligeVVGSGFRGNYFEGPAVFKRNGYYYLIYSAGGTDGGYAIGYATSDSPLGPWEKASHNPGLQ 221

                         250       260       270
                  ....*....|....*....|....*....|.
gi 1266971053 306 NSNDiLTSFHSQPTYILKVKSDKYIYLGDRW 336
Cdd:cd08978   222 TSGA-TGIYGPGHGSIFQDEGDRWYIVYHAR 251
XynB2 COG3507
Beta-xylosidase [Carbohydrate transport and metabolism];
89-315 4.54e-21

Beta-xylosidase [Carbohydrate transport and metabolism];


Pssm-ID: 442730 [Multi-domain]  Cd Length: 351  Bit Score: 93.09  E-value: 4.54e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVLD-LSHLPENYQ--LERPKIIYnkKNKEYVMWFhleKNKKFTDGSGEAGVATSNNITGPYTFIS 165
Cdd:COG3507    58 IFHSKDLVNWELVGHALDrLPQWADPYSggIWAPDIRY--HNGKYYLYY---TAVDGGKNRSGIGVATADDPEGPWSDPG 132

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 166 AsysnagvkpfspaantdssnwfvrqankrfekYFSRGQPVRDMALFVDDDEKAYLIYESEDNYsLQVARLSDDYKSFSG 245
Cdd:COG3507   133 P--------------------------------LVCPGGNGIDPSVFVDDDGKAYLVYGSGGGG-IYVAELDPDTGKLLG 179

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1266971053 246 VYSRIL---VGQLNEAPSIMKYKNKYYLITS-GLSGYRPNAARISVADNILGPWVTL-GNPMSSFNSNDILTSFH 315
Cdd:COG3507   180 EPKTLApggEGGWIEGPHIYKRNGYYYLFYSeGGTCNSGYAVRVARSKSPTGPYEDApGNPILTQRSDGGIQGPG 254
GH43_AXH_like cd08990
Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, ...
 78-308 3.14e-18

Glycosyl hydrolase family 43 protein, includes arabinoxylan arabinofuranohydrolase, beta-xylosidase, endo-1,4-beta-xylanase, and alpha-L-arabinofuranosidase; This subgroup includes Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160), Butyrivibrio proteoclasticus alpha-L-arabinofuranosidase (Xsa43E;bpr_I2319), Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and metagenomic beta-xylosidase (EC 3.2.1.37) / alpha-L-arabinofuranosidase (EC 3.2.1.55) CoXyl43. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_AXH-like subgroup includes enzymes that have been characterized with beta-xylosidase, alpha-L-arabinofuranosidase, endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Metagenomic beta-xylosidase/alpha-L-arabinofuranosidase CoXyl43 shows synergy with Trichoderma reesei cellulases and promotes plant biomass saccharification by degrading xylo-oligosaccharides, such as xylobiose and xylotriose, into the monosaccharide xylose. Studies show that the hydrolytic activity of CoXyl43 is stimulated in the presence of calcium. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350104 [Multi-domain]  Cd Length: 269  Bit Score: 83.42  E-value: 3.14e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  78 NEWQgsnqkvnMYRSKDLVHWMPVGIVLDLSHLP--ENYQLERPKIIYnkKNKEYVMWF-HLEKNKKFtdgsgEAGVATS 154
Cdd:cd08990    30 DDWH-------VFSSTDLVNWTDHGEILPPDDVFwwASGNAWAPDAVY--KNGKYYFYFpVGQASDGF-----GIGVAVS 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 155 NNITGPYTFisasysnAGVKPFSPAANTDSSNWfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYESEDNYslQVA 234
Cdd:cd08990    96 DSPAGPFKD-------ALGKPLIPEGLNGIEGI--------------------DPAVFVDDDGRAYLYFGGGGGY--YVA 146

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1266971053 235 RLSDDYKSFSGVYSRILVGQL---NEAPSIMKYKNKYYLITSGLSGYrPNAARISVADNILGPWVTLGNPMSSFNSN 308
Cdd:cd08990   147 KLKDDMISLAGEPQKIKNGGLkgfFEAPWVFKRNGTYYLSYAGGWAY-PAEIAYSTADSPLGPYTYRGVILDPVGSG 222
GH43-like cd08986
Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43) ...
 78-295 1.47e-14

Glycosyl hydrolase family 43 protein; uncharacterized; This glycosyl hydrolase family 43 (GH43)-like subfamily includes uncharacterized enzymes similar to those with beta-1,4-xylosidase (xylan 1,4-beta-xylosidase; EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanase and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350100 [Multi-domain]  Cd Length: 257  Bit Score: 72.65  E-value: 1.47e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  78 NEWQGSNQKVNMYRSKDLVHWMPVGIVLDLSHLPENYQLERPKIIYNKKNKEyvMW---FHLEKNKKF-----TDGSGEA 149
Cdd:cd08986    24 PDWWGVNDGIRLWRSKDLKDWEYLGLVWDLEKDGWWQWEPQWWTPDSKNKRA--LWapeIHYINGTWYithsmNGGGTGL 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 150 GVATSNNITGPYTFISASYSNAGVKPFspaantdssnwfvrqankrfekyfsrgqpvrdmaLFVDDDEKAYLIYeseDNY 229
Cdd:cd08986   102 LKSTTGKPEGPYVDPMGGPLGKGIDPS----------------------------------LFEDDDGTVYLVW---GNG 144

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1266971053 230 slQVARLSDDYKSFSGVYSRILVGQLN----EAPSIMKYKNKYYLITSGLSGYRPNAAR----ISVADNILGPW 295
Cdd:cd08986   145 --QIARLKKDMSGFAEEPRKIDPSGNReighEGAFIFKIGGKYVLFGAAWSTDKMRKGTydlyYATSDSIYGPY 216
GH43_bXyl-like cd09004
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
 78-295 5.44e-14

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675) and (BT3662;BT_3662); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350118 [Multi-domain]  Cd Length: 266  Bit Score: 71.10  E-value: 5.44e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  78 NEWQGSNQKVnmYRSKDLVHWMPVGIVLDLSH--LPENYQLERPKIIynKKNKEYVMWFHLEKNkkftdgsgeAGVATSN 155
Cdd:cd09004    22 PGWSSTSFHV--FSSTDLVNWTDHGIILDLANdvWWANKGAWAPAVA--ERNGKYYFYFSAGSQ---------IGVAVSD 88

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 156 NITGPYTfisasysnagvkpfspaantDSSNWFVRqankrfeKYFSRGQPVrDMALFVDDDEKAYLIYeseDNYSLQVAR 235
Cdd:cd09004    89 SPTGPFT--------------------DLGRPLVT-------GGDYGGQAI-DPMVFVDDDGQAYLYW---GNGTAYVAR 137

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1266971053 236 LSDDYKSFSGVYSRILV-GQLNEAPSIMKYKNKYYLITSGLSGYRPN-AARISVADNILGPW 295
Cdd:cd09004   138 LNDDMVSFDGEVVVSITpPNFREGPFVHKRNGIYYLSWSENDTRDPDyRVRYATSDSPLGPW 199
GH43_FsAxh1-like cd09001
Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 ...
 91-295 8.15e-13

Glycosyl hydrolase family 43 such as Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase; This glycosyl hydrolase family 43 (GH43) includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase; xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. This subfamily includes the characterized Clostridium stercorarium F-9 beta-xylosidase Xyl43B. It also includes Humicola insolens AXHd3 (HiAXHd3), a GH43 arabinofuranosidase (EC 3.2.1.55) that hydrolyzes O3-linked arabinose of doubly substituted xylans, a feature of the polysaccharide that is recalcitrant to degradation. It possesses an additional C-terminal beta-sandwich domain such that the interface between the domains comprises a xylan binding cleft that houses the active site pocket. The HiAXHd3 active site is tuned to hydrolyze arabinofuranosyl or xylosyl linkages, and the topology of the distal regions of the substrate binding surface confers specificity. It also includes Fibrobacter succinogenes subsp. succinogenes S85 arabinoxylan alpha-L-arabinofuranosidase (Axh1;Fisuc_1769;FSU_2269), Paenibacillus sp. E18 alpha-L-arabinofuranosidase (Abf43A), Bifidobacterium adolescentis ATCC 15703 double substituted xylan alpha-1,3-L-specific arabinofuranosidase d3 (AXHd3;AXH-d3;BaAXH-d3;BAD_0301;E-AFAM2), and Chrysosporium lucknowense C1 arabinoxylan hydrolase / double substituted xylan alpha-1,3-L-arabinofuranosidase (Abn7;AXHd). A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350115 [Multi-domain]  Cd Length: 270  Bit Score: 67.93  E-value: 8.15e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  91 RSKDLVHWMPVGIVLD-LSHLPEnYQLERPKIIYNKKnkeyvMW-----FHlekNKKF----TDGSGEAGVATSNNITGP 160
Cdd:cd09001    40 HSKDLVNWEIVGYVVDrLDDGDA-YYLEDGKNAYGKG-----IWapslrYH---NGKFyvyfCTNTGGTYVYTADDPAGP 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 161 YTFISasysnagvkpfspaanTDSSNWFvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYESEDNYslqVARLSDDY 240
Cdd:cd09001   111 WSRPA----------------LIGKGYH-------------------DPSLLFDDDGKAYLVYGNGEIR---LTELSPDG 152

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1266971053 241 KSFSG----VYSRILVGQLNEAPSIMKYKNKYYLITSGlSGYRPNAARISVADNILGPW 295
Cdd:cd09001   153 TGVGGegrvIIDGTEEGLGAEGSHLYKINGYYYIFNIE-WGGGGRTQVVLRSKSLYGPY 210
Glyco_hydro_43 pfam04616
Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are ...
 77-309 2.43e-12

Glycosyl hydrolases family 43; The glycosyl hydrolase family 43 contains members that are arabinanases. Arabinanases hydrolyse the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 398349 [Multi-domain]  Cd Length: 281  Bit Score: 66.57  E-value: 2.43e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  77 SNEWQGsnqKVNMYRSKDLVHWMPVGIVLD-LSHLPENYQLER--PKIIYnkKNKEYVMWFhleknkkfTDGSGEAGVAT 153
Cdd:pfam04616  28 SFEWFP---GIPIFHSKDLVNWKLVGPVLVrRSQLSGRGSNASwaPDISY--HDGKYYLYY--------TAVAHGIFVAT 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 154 SNNITGPYTfisasysnagvKPFSPAANTDSSnwfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYESED----NY 229
Cdd:pfam04616  95 ADSPDGPWS-----------DPGKLKSGGGGI----------------------DPSLFHDDDGKKYLVWGGWDprhgHG 141

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 230 SLQVARLSDDYKSFSGVYSRILV-------GQLNEAPSIMKYKNKYYLITS-GLSGYrPNAARISVADNILGPWV-TLGN 300
Cdd:pfam04616 142 GIYLQELDNDGLKLVGPVTKLIYpgtrwvgGKVTEGPHLYKRNGYYYLTYAaGGTGG-PYAVGVARSRSPLGPYEwHPGN 220


                  ....*....
gi 1266971053 301 PMSSFNSND 309
Cdd:pfam04616 221 PILTSRSPE 229
GH43_HoAraf43-like cd08991
Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 ...
 90-296 5.73e-12

Glycosyl hydrolase family 43 protein such as Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (HoAraf43;Hore_20580); This glycosyl hydrolase family 43 (GH43) subgroup includes Halothermothrix orenii H 168 alpha-L-arabinofuranosidase (EC 3.2.1.55) (HoAraf43;Hore_20580). It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. This GH43_ HoAraf43-like subgroup includes enzymes that have been annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350105 [Multi-domain]  Cd Length: 283  Bit Score: 65.66  E-value: 5.73e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  90 YRSKDLVHWMPVGIVLDLSHLPENYQLERPKIIYNkkNKEYVMWF---HLEKNKKFtdgsgeaGVATSNNITGPYTfisa 166
Cdd:cd08991    28 YVSDDLVNWEYPGGALEEPGLWGTKGFWAPEVFYY--NGKFYMYYsanGGDHGEHI-------AVAVSDSPLGPFR---- 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 167 sysNAGVKPFSPAANT-DSSnwfvrqankrfekyfsrgqpvrdmaLFVDDDEKAYLIY-----ESEDNYSLQVARLSDDY 240
Cdd:cd08991    95 ---DKGKLLIPAGGFSiDAH-------------------------VFIDDDGKWYLYYvrddlGGEPGNRIYVAELEDDL 146

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1266971053 241 KSFSG-------------VYSRILVGQLNEAPSIMKYKNKYYLITSGlSGYR-PN-AARISVADNILGPWV 296
Cdd:cd08991   147 SLIGEptlvlcptaderwEYGEGRDWHTTEGPTVLKHNGTYYLTYSA-NHFRsPDyAVGYATADSPLGPWT 216
GH43_ABN-like cd18616
Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl ...
 89-307 5.98e-11

Glycosyl hydrolase family 43 such as arabinan endo-1 5-alpha-L-arabinosidase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activity. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350128 [Multi-domain]  Cd Length: 291  Bit Score: 62.59  E-value: 5.98e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVLDLS---HLPENYQLERPKIIYnkKNKEYVMWFHLeknkkfTDGSGEA----GVATSNNITGPY 161
Cdd:cd18616    41 ILRSKDLVNWEYVGDAFTSKprwKWDPGGGLWAPDIRY--IDGKYVLYYSL------SDWGADPnpgiGVATADSPAGPF 112

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 162 TfisasysNAGvKPFSPAaNTDSSNWFvrqankrfekyfsrgqpvrDMALFVDDDeKAYLIYESedNYSLQVARLSDDYK 241
Cdd:cd18616   113 T-------DQG-KLFDSN-EIGVRNSI-------------------DPFVFEDDG-KKYLFWGS--FYGIYAVELTADGL 161

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1266971053 242 SFSGVYSRILVGQLNEAPSIMKYKNKYYLITS-------GLSGYRPNAARisvADNILGPWVT-LGNPMSSFNS 307
Cdd:cd18616   162 ALKPGEKVQIAGDRYEGPYIVKRDGYYYLFGSagsccegPNSTYRVVVGR---SESLLGPYVDrDGRSLLDSGG 232
GH43_ABN-like cd08999
Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase ...
 81-301 1.23e-10

Glycosyl hydrolase family 43 protein such as endo-alpha-L-arabinanase; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350113 [Multi-domain]  Cd Length: 284  Bit Score: 61.39  E-value: 1.23e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  81 QGSNQKVNMYRSKDLVHWMPVGIvlD-LSHLPENYQLER----PKIIYNKKNKeYVMWFhleknkkftdgSGEA------ 149
Cdd:cd08999    26 NSGGKNVQVATSTDLVTWTLLGG--DaLPDLPAWAAAGGntwaPDVVRRPDGK-YVMYY-----------SARLkssgkh 91

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 150 --GVATSNNITGPYTfisasysNAGVKPFSPAANTDSSnwfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYESED 227
Cdd:cd08999    92 ciGVATSDSPLGPFT-------PVGEPPLCPLDQGGAI----------------------DPSGFVDPDGKRYLVYKVDG 142

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 228 NY-----SLQVARLSDDYKSFSGVYSRILVGQLN------EAPSIMKYKNKYYLITSG----LSGYRPNAARisvADNIL 292
Cdd:cd08999   143 NSigvptPIMLQELSADGLTLVGEPVELLLNDGPwdgplvEAPSLVKRDGTYYLFYSSncycSPSYAVGYAT---SKSIT 219


                  ....*....
gi 1266971053 293 GPWVTLGNP 301
Cdd:cd08999   220 GPYTKAGEP 228
GH43_F5-8_typeC-like cd18608
Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 ...
 89-300 8.30e-10

Glycosyl hydrolase family 43 protein most having a F5/8 type C domain C-terminal to the GH43 domain; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), xylanase (EC 3.2.1.8), and beta-galactosidase (EC 3.2.1.145) activities, and some as F5/8 type C domain (also known as the discoidin (DS) domain)-containing proteins. Most contain a F5/8 type C domain C-terminal to the GH43 domain. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. Characterized enzymes belonging to this subgroup include Lactobacillus brevis (LbAraf43) and Weissella sp (WAraf43) which show activity with similar catalytic efficiency on 1,5-alpha-L-arabinooligosaccharides with a degree of polymerization (DP) of 2-3; size is limited by an extended loop at the entrance to the active site. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350120 [Multi-domain]  Cd Length: 276  Bit Score: 58.83  E-value: 8.30e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVLDLSHLPENYQLERPKIIYNKKNKEYVMWfhleknkkfTDGSgEAGVATSNNITGPYTFISASY 168
Cdd:cd18608    32 VWKSKDFVNWKFEGLNWPTKAASGDSKVWAPSVVKGKDGKYYMYV---------SVGS-EIYVGVADSPLGPWKNANGDG 101

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 169 SNAGVKPFSPAANTdssnwfvrqankrfekyfsrgqpvRDMALFVDDDEKAYLIYESEDNYSLQ--VARLSDDYKSFSGv 246
Cdd:cd18608   102 PPIIPGDGKPNYHM------------------------IDAEPFIDDDGKAYLYWGSGLHVNGHcfAAKLNPDMVTFDG- 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1266971053 247 ySRILVGQLN---EAPSIMKYKNKYYLITSGLSGYRPN-AARISVADNILGPWVTLGN 300
Cdd:cd18608   157 -SEPTIVTPRdyfEAPFMFKRNGIYYLMYSGGGCWDETyNVRYAVSDNPLGPFEEGEN 213
GH43_XynD-like cd09003
Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan ...
 82-308 9.11e-10

Glycosyl hydrolase family 43 protein such as Bacillus subtilis arabinoxylan arabinofuranohydrolase (XynD;BsAXH-m23;BSU18160); This glycosyl hydrolase family 43 (GH43) subgroup includes characterized Bacillus subtilis arabinoxylan arabinofuranohydrolase (AXH), Caldicellulosiruptor sp. Tok7B.1 beta-1,4-xylanase (EC 3.2.1.8) / alpha-L-arabinosidase (EC 3.2.1.55) XynA, Caldicellulosiruptor sp. Rt69B.1 xylanase C (EC 3.2.1.8) XynC, and Caldicellulosiruptor saccharolyticus beta-xylosidase (EC 3.2.1.37)/ alpha-L-arabinofuranosidase (EC 3.2.1.55) XynF. It belongs to the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. It belongs to the GH43_AXH-like subgroup which includes enzymes that have been annotated as having beta-xylosidase, alpha-L-arabinofuranosidase and arabinoxylan alpha-L-1,3-arabinofuranohydrolase, xylanase (endo-alpha-L-arabinanase) as well as AXH activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. Bacillus subtilis AXH (BsAXH-m2,3) has been shown to cleave arabinose units from O-2- or O-3-mono-substituted xylose residues and superposition of its structure with known structures of the GH43 exo-acting enzymes, beta-xylosidase and alpha-L-arabinanase, each in complex with their substrate, reveals a different orientation of the sugar backbone. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350117 [Multi-domain]  Cd Length: 315  Bit Score: 59.20  E-value: 9.11e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  82 GSNQKVNMYRSKDLVHWMPVGIVLDLShlpENYQLER------PKIIYNKKNKeyvmwfhleKNK---KFTDGSGEAGVA 152
Cdd:cd09003    44 YNINSLTVISSDDMVNWTDHGEIPVAG---PNGIAKWagnswaPSVAYKNING---------KDKfylYFANGGGGIGVL 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 153 TSNNITGPYT-------FISASYSNAGVK-PFSPAAntdssnwfvrqankrfekyfsrgqpvrdmalFVDDDEKAYLIY- 223
Cdd:cd09003   112 TADSPTGPWTdplgkplITRSTPGCAGVVwLFDPAV-------------------------------FIDDDGQGYLYFg 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 224 ------ESEDNYSLQVARLSDDYKSFSGVYSRILVGQLNEAPSIMKYKNKYYL-----ITSGLSGYRPNAARIS--VADN 290
Cdd:cd09003   161 ggvpggSEANPKTARVIKLGDDMISVDGSAVTIDAPYFFEASGINKINGKYYYsyctnFSGRDDPAYPGAGSIAymTSDN 240

                         250       260
                  ....*....|....*....|..
gi 1266971053 291 ILGPW----VTLGNPMSSFNSN 308
Cdd:cd09003   241 PMGPFtykgVILKNPGTFFGNG 262
GH43_XylA-like cd18620
Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium ...
 94-296 1.03e-09

Glycosyl hydrolase family 43-like protein such as Clostridium stercorarium alpha-L-arabinofuranosidase XylA; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. The GH43_XylA-like subgroup includes Clostridium stercorarium alpha-L-arabinofuranosidase XylA, and enzymes that have been annotated as having beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), endo-alpha-L-arabinanase (EC 3.2.1.-) as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan.


Pssm-ID: 350132 [Multi-domain]  Cd Length: 274  Bit Score: 58.76  E-value: 1.03e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  94 DLVHWMPVGIVLD-----LSHLPENYQLERPKIIYnkKNKEYVMWFhleknkkFTDGSGEAGVATSNNITGPYTFISasy 168
Cdd:cd18620    40 DLSNWRYHGVIFRsdqdpDEVPPGKGLLYAPDVVK--GPGRYYLYY-------CLSKGSVEGVAVSDSPAGPFEYLG--- 107

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 169 snagvkpfspaantdssnwFVRqaNKRFEKYFSRgqpvrDMALFVDDDEKAYLIYESEdnySLQVARLSDDYKSFSGVYS 248
Cdd:cd18620   108 -------------------PVK--YPRKGDIFQI-----DPAVLVDDDGRVYLYWGQG---GSKGAELDPDMLTIKPETI 158

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1266971053 249 RILVGQLN-------EAPSIMKYKNKYYLITSGLSGYRPNAARISVADNILGPWV 296
Cdd:cd18620   159 VDVPAGITfeghgffEGSSIRKINGIYYLVYSSISRGRPTELCYATSKSPLGPFT 213
GH43_BT3675-like cd18828
Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 ...
 80-329 2.58e-09

Glycosyl hydrolase family 43 protein such as Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (BT3675;BT_3675); This glycosyl hydrolase family 43 (GH43) subgroup includes the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). It belongs to the GH43_bXyl subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl subgroup also includes enzymes annotated as having xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase, EC 3.2.1.8) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350149 [Multi-domain]  Cd Length: 283  Bit Score: 57.67  E-value: 2.58e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  80 WQGSNQKVnmYRSKDLVHWMPVGIVLDL--SHLPE--NYQLERPKIIynKKNKEYVMWFhlekNKKFTDGSGEAGVATSN 155
Cdd:cd18828    24 WSGTQFHV--FSSDDLVTWKDEGVILDLknDQVVPwaTGNAWAPTIE--ERDGKYYFYF----CGKNPDGRSQIGVAVAD 95

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 156 NITGPYTFISasysnagvkpfSPAANTDSSNwfvrqankrfekyFSRGQPVrDMALFVDD-DEKAYLIYeseDNYSLQVA 234
Cdd:cd18828    96 SPTGPFTAQG-----------SPLITHEMAR-------------VTMGQAI-DPSVFTDPvDGKYYLYW---GNGYAAIA 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 235 RLSDDYKSFSGVYSRILVG--QLNEAPSIMKYKNKYYLITS----GLSGYRPNAArisVADNILGPWVTLGNPMSSFNSN 308
Cdd:cd18828   148 ELNDDMISIKPGTLVNLDGltDFREAVTVLYRDGLYHFTWScddtGSENYHVNYG---TSDSPYGPITYRGVILQKDPSK 224

                         250       260
                  ....*....|....*....|...
gi 1266971053 309 DIL-TSFHSqptyILKVK-SDKY 329
Cdd:cd18828   225 GILgTGHHS----ILQVPgTDEW 243
GH43_XlnD-like cd18827
Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); ...
 89-299 3.14e-08

Glycosyl hydrolase family 43 protein such as Aspergillus niger DMS1957 xylanase D (XlnD); includes mostly xylanases; This glycosyl hydrolase family 43 (GH43) subgroup includes enzymes that have mostly been annotated as xylanases (endo-alpha-L-arabinanase, EC 3.2.1.8). It belongs to the GH43_bXyl-like subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. The GH43_bXyl-like subgroup includes enzymes that have been annotated as xylan-digesting beta-xylosidases (EC 3.2.1.37) and xylanases, as well the Bacteroides thetaiotaomicron VPI-5482 alpha-L-arabinofuranosidases (EC 3.2.1.55) (BT3675;BT_3675) and (BT3662;BT_3662). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350148 [Multi-domain]  Cd Length: 277  Bit Score: 54.21  E-value: 3.14e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVLDLSHLP-ENYQLERPKIIynKKNKEYVMWFHLEKNKKFTDGSGeAGVATSNNITGPYtfisas 167
Cdd:cd18827    30 AFSSPDLVHWTKHERILDMADVPwANRAVWAPSVI--EKNGKYYLYFAANDIQSDDEGGG-IGVAVADRPEGPF------ 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 168 ySNAGVKPFSpaantdssNWFVRQAnkrfekyfsrgQPVrDMALFVDDDEKAYLIY--ESEDNyslqVARLSDDYKSF-- 243
Cdd:cd18827   101 -KDALGKPLI--------GEFHNGA-----------QPI-DQHVFKDDDGQAYLYYggWGHCN----VAKLNDDMTSLvp 155

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1266971053 244 ---SGVYSRILVGQLNEAPSIMKYKNKYYLITSgLSGYRPNAARIS--VADNILGPWVTLG 299
Cdd:cd18827   156 fddGETFKEITPEGYVEGPFMFKRNGKYYFMWS-EGGWTGPDYSVAyaVADSPLGPFKRIG 215
GH43_XYL-like cd08989
Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl ...
 92-295 3.44e-08

Glycosyl hydrolase family 43, beta-D-xylosidases and arabinofuranosidases; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes that have been annotated as having beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium beta-D-xylosidase SXA. These are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. It also includes various GH43 family GH43 arabinofuranosidases (EC 3.2.1.55) including Humicola insolens alpha-L-arabinofuranosidase AXHd3, Bacteroides ovatus alpha-L-arabinofuranosidase (BoGH43, XynB), and the bifunctional Phanerochaete chrysosporium xylosidase/arabinofuranosidase (Xyl;PcXyl). GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350103 [Multi-domain]  Cd Length: 272  Bit Score: 54.29  E-value: 3.44e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  92 SKDLVHWMPVGIV------LDLSHLPENYQLERPKIIYNkkNKEYVMWFHLEKNKKFTDGSGEAgVATSNNITGPYTfis 165
Cdd:cd08989    38 SKDLVHWTPIGHAltrpeqLDLTGGPDGGGIWAPDISYH--DGKFYIYYTVVLNVGSWKGRRNY-LVTSEDPEGPWS--- 111

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 166 asysnagvkpfSPAANTDSSNwfvrqankrfekyfsrgqpvrDMALFVDDDEKAYLIYeseDNYSLQVARLSDDYKSFSG 245
Cdd:cd08989   112 -----------EPVWLDEGGI---------------------DPSLFVDDDGKHYMLL---NPGGIRLAELNPDCTKQIG 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 1266971053 246 VYSRILVG---QLNEAPSIMKYKNKYYLITS-GLSGYrpnAARISVA--DNILGPW 295
Cdd:cd08989   157 EPKRIWEGtggRAPEGPHLYKKDGYYYLLTAeGGTGY---GHAITIArsKTIYGPY 209
GH43_Xsa43E-like cd18618
Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase ...
 150-295 2.37e-07

Glycosyl hydrolase family 43, including Butyrivibrio proteoclasticus arabinofuranosidase Xsa43E; This glycosyl hydrolase family 43 (GH43) subgroup belongs to the GH43_AXH-like subgroup which includes enzymes that have been characterized with beta-xylosidase (EC 3.2.1.37), alpha-L-arabinofuranosidase (EC 3.2.1.55), alpha-1,2-L-arabinofuranosidase 43A (arabinan-specific; EC 3.2.1.-), endo-alpha-L-arabinanase as well as arabinoxylan arabinofuranohydrolase (AXH) activities. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. AXHs specifically hydrolyze the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl backbone residues of arabinoxylan. This subgroup includes Cellvibrio japonicus arabinan-specific alpha-1,2-arabinofuranosidase, CjAbf43A, which confers its specificity by a surface cleft that is complementary to the helical backbone of the polysaccharide, and Butyrivibrio proteoclasticus GH43 enzyme Xsa43E, also an arabinofuranosidase, which has been shown to cleave arabinose side chains from short segments of xylan. Several of these enzymes also contain carbohydrate binding modules (CBMs) that bind cellulose or xylan. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350130 [Multi-domain]  Cd Length: 275  Bit Score: 51.45  E-value: 2.37e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 150 GVATSNNITGPYTfisasysNAGVKPFSPAANTDSSNWFVRQAnkrfekyfsrgqpvrDMALFVDDDEKAYLIYeseDNY 229
Cdd:cd18618    94 GVAVSDSPTGPFK-------DALGKPLITNDMTGTTNHSWDDI---------------DPTVFIDDDGQAYLYW---GNP 148

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1266971053 230 SLQVARLSDDYKSFSGVYSRILVGQL---NEAPSIMKYKNKYYLITSglSGYrPNAARISVADNILGPW 295
Cdd:cd18618   149 ELYYVKLKEDMISLDGEIGTIDISGLpdfTEAPWVHKRNGLYYLSYA--AGF-PEKIAYATSDSPTGPW 214
GH43_GsAbnA-like cd18832
Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1, ...
 91-343 5.43e-06

Glycosyl hydrolase family 43 protein such as Geobacillus stearothermophilus endo-alpha-1,5-L-arabinanase AbnA; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. It includes Geobacillus stearothermophilus T-6 NCIMB 40222 AbnA, Bacillus subtilis subsp. subtilis str. 168 (Abn2;YxiA;J3A;BSU39330) (Arb43B), and Thermotoga petrophila RKU-1 (AbnA;TpABN;Tpet_0637). These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. Many of these enzymes are different from other arabinases; they are organized into two different domains with a divalent metal cluster close to the catalytic residues to guarantee the correct protonation state of the catalytic residues and consequently the enzyme activity. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350153 [Multi-domain]  Cd Length: 332  Bit Score: 47.63  E-value: 5.43e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  91 RSKDLVHWMPVG--------IVLDLSHLPENYQLE-----------RPKIIYNKKNKEYVMWFHLEknkkfTDGSGEA-G 150
Cdd:cd18832    24 KSTDLMNWTQFTngvttdnpLLFNLFDSTAWELAEdfnwagggnlwAPDVIYNKAMGKYCMYYSVS-----GDDSPSAiG 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 151 VATSNNITGPYTFISA-SYSNAGVKPFSPAANTDSSNwfvrqankrfeKYFSRGQP-VRDMALFVDDDEKAYLIYES--- 225
Cdd:cd18832    99 LATADNIEGPYTYKGTvLKSGFTGSTSADADVYLTGG-----------KYNNNYHPnAIDPCVFYDKDGKLWMVYGSwsg 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 226 -------------EDNYSLQVARLSDDY---KSFSGVYSRIlvgqlnEAPSIMKYKNK--YYLITS--GLS---GYRpna 282
Cdd:cd18832   168 giflleldpktglRDYSVETDGNLPDQYygkKIAGGYHASG------EGPYILYDKDTgyYYLFVSygGLDangGYN--- 238

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1266971053 283 ARISVADNILGPWV-TLGNPMSSFNSNdiltsfhsqPTYILKVKSDKYIYLGDRWDFKNNSY 343
Cdd:cd18832   239 IRVFRSKNPDGPYVdAAGNDAIYTSGN---------DNLGLKDGGIIELMGNYKLPGLGTGY 291
GH43_SXA-like cd09000
Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This ...
 204-323 3.41e-05

Glycosyl hydrolase family 43, such as Selenomonas ruminantium beta-D-xylosidase SXA; This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized to mainly have beta-1,4-xylosidase (beta-D-xylosidase;xylan 1,4-beta-xylosidase; EC 3.2.1.37) activity, including Selenomonas ruminantium (Xsa;Sxa;SXA), Bifidobacterium adolescentis ATCC 15703 (XylC;XynB;BAD_0428) and Bacillus sp. KK-1 XylB. They are part of an array of hemicellulases that are involved in the final breakdown of plant cell-wall whereby they degrade xylan. They hydrolyze beta-1,4 glycosidic bonds between two xylose units in short xylooligosaccharides. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. These enzymes possess an additional C-terminal beta-sandwich domain that restricts access for substrates to a portion of the active site to form a pocket. The active-site pockets comprise of two subsites, with binding capacity for two monosaccharide moieties and a single route of access for small molecules such as substrate. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350114 [Multi-domain]  Cd Length: 292  Bit Score: 45.23  E-value: 3.41e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 204 QPVR------DMALFVDDDEKAYLIYESEDN-------YSLQVARLSDDYKSFSGVYSRILVG---QLNEAPSIMKYKNK 267
Cdd:cd09000   113 EPIYlnssgfDPSLFHDDDGRKYLVNMLWDHrpghnrfAGIVLQEFDPETKKLVGERKVIFKGtelGLTEGPHLYKRDGY 192

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1266971053 268 YYLITS-GLSGYRpNAARISVADNILGPW-VTLGNPMssfnsndiLTSFHSQPTYILK 323
Cdd:cd09000   193 YYLLTAeGGTGYE-HAVTVARSRNIFGPYeVDPDNPL--------LTSWDDPENPLQK 241
GH43_62_32_68_117_130-like cd08994
Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl ...
 259-369 2.23e-04

Glycosyl hydrolase families: GH43, GH62, GH32, GH68, GH117, CH130; Members of the glycosyl hydrolase families 32, 43, 62, 68, 117 and 130 (GH32, GH43, GH62, GH68, GH117, GH130) all possess 5-bladed beta-propeller domains and comprise clans F and J, as classified by the carbohydrate-active enzymes database (CAZY). Clan F consists of families GH43 and GH62. GH43 includes beta-xylosidases (EC 3.2.1.37), beta-xylanases (EC 3.2.1.8), alpha-L-arabinases (EC 3.2.1.99), and alpha-L-arabinofuranosidases (EC 3.2.1.55), using aryl-glycosides as substrates, while family GH62 contains alpha-L-arabinofuranosidases (EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose sidechains from xylans. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Clan J consists of families GH32 and GH68. GH32 comprises sucrose-6-phosphate hydrolases, invertases (EC 3.2.1.26), inulinases (EC 3.2.1.7), levanases (EC 3.2.1.65), eukaryotic fructosyltransferases, and bacterial fructanotransferases while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), while GH68 consists of frucosyltransferases (FTFs) that include levansucrase (EC 2.4.1.10); beta-fructofuranosidase (EC 3.2.1.26); inulosucrase (EC 2.4.1.9), all of which use sucrose as their preferential donor substrate. Members of this clan are retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) that catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. Structures of all families in the two clans manifest a funnel-shaped active site that comprises two subsites with a single route for access by ligands. Also included in this superfamily are GH117 enzymes that have exo-alpha-1,3-(3,6-anhydro)-l-galactosidase activity, removing terminal non-reducing alpha-1,3-linked 3,6-anhydro-l-galactose residues from their neoagarose substrate, and GH130 that are phosphorylases and hydrolases for beta-mannosides, involved in the bacterial utilization of mannans or N-linked glycans.


Pssm-ID: 350108 [Multi-domain]  Cd Length: 294  Bit Score: 42.63  E-value: 2.23e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 259 PSIMKYKNKYYLITSGLSGYRPNAA---------RISVA--DNILGPWVTLGNPM---SSFNSNDILTS-----FHSQPT 319
Cdd:cd08994    83 PTIKKFDGKYYLYYIGNTGPGPDPPlwwghrnnqRIGVAvaDSPNGPWKRFDKPIldpRPRSWDDLITSnpavlKRPDGS 162

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1266971053 320 YILKVKSDKYIYLGDRWDFKnnSYSDLWKSTYVWLPINFSNEIPSISWVE 369
Cdd:cd08994   163 YLLYYKGGKKNPGGNRKHGV--AVSDSPEGPYTKLSDPPVYEPGVNGQTE 210
GH43_ABN cd08988
Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes ...
 89-273 7.32e-04

Glycosyl hydrolase family 43; This glycosyl hydrolase family 43 (GH43) subgroup includes mostly enzymes with alpha-L-arabinofuranosidase (ABF; EC 3.2.1.55) and endo-alpha-L-arabinanase (ABN; EC 3.2.1.99) activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. The GH43 ABN enzymes hydrolyze alpha-1,5-L-arabinofuranoside linkages while the ABF enzymes cleave arabinose side chains so that the combined actions of these two enzymes reduce arabinan to L-arabinose and/or arabinooligosaccharides. These arabinan-degrading enzymes are important in the food industry for efficient production of L-arabinose from agricultural waste; L-arabinose is often used as a bioactive sweetener. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.


Pssm-ID: 350102 [Multi-domain]  Cd Length: 277  Bit Score: 40.96  E-value: 7.32e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053  89 MYRSKDLVHWMPVGIVL-----DLSHLPENYQ--LERPKIIYNKKnkEYVMWFHLEKNKKFTDGsgeAGVATSNNITGPY 161
Cdd:cd08988    26 IAKSKDLGNWTIVGEAFatlpsWKGGSPPSADgnLWAPDISQHKG--KYYLYYSVSDNGSNTSA---IGLATANNPQGPF 100

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1266971053 162 TfisasySNAGVKPFSPAANTDSSnwfvrqankrfekyfsrgqpvRDMALFVDDDEKAYLIYESeDNYSLQVARLSDD-- 239
Cdd:cd08988   101 K------DEGPAKPVVTSDNAGNA---------------------IDPDLFQDEDGQNWLLYGS-FWGGIWLQKLDKNgl 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 1266971053 240 -------YKSFSGVYSRILvgqlnEAPSIMKYKNKYYLITS 273
Cdd:cd08988   153 vvnppgnGKSIAVLYYVSI-----EAPYITYAGGYYYLFVS 188
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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