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Conserved domains on  [gi|2475457614|emb|CAI8267316|]
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MAG: (R)-stereoselective amidase [Owenweeksia sp. TMED14]

Protein Classification

carbon-nitrogen hydrolase family protein( domain architecture ID 27728)

carbon-nitrogen hydrolase family protein similar to nitrilase, which is involved in the reduction of organic nitrogen compounds and ammonia production, breaks carbon-nitrogen bonds and depends on a Glu-Lys-Cys catalytic triad

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
nitrilase super family cl11424
Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing ...
 5-148 2.36e-69

Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes; This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds; it includes nitrilases, cyanide dihydratases, aliphatic amidases, N-terminal amidases, beta-ureidopropionases, biotinidases, pantotheinase, N-carbamyl-D-amino acid amidohydrolases, the glutaminase domain of glutamine-dependent NAD+ synthetase, apolipoprotein N-acyltransferases, and N-carbamoylputrescine amidohydrolases, among others. These enzymes depend on a Glu-Lys-Cys catalytic triad, and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. These oligomers include dimers, tetramers, hexamers, octamers, tetradecamers, octadecamers, as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism, in detoxifying small molecules, in the synthesis of signaling molecules, and in the post-translational modification of proteins. They are used industrially, as biocatalysts in the fine chemical and pharmaceutical industry, in cyanide remediation, and in the treatment of toxic effluent. This superfamily has been classified previously in the literature, based on global and structure-based sequence analysis, into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative, the plasmid-borne TraB family, has not been included in the hierarchy.


The actual alignment was detected with superfamily member cd07575:

Pssm-ID: 448250  Cd Length: 252  Bit Score: 210.09  E-value: 2.36e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   5 LQITALQIPLFCEAPALNRKSIDENLTKLHG-TDILLLPEMFTSGFTMNPSTVAESMDGPTIKWMKSWAYSLDAAVGGSI 83
Cdd:cd07575     1 LKIALIQTDLVWEDPEANLAHFEEKIEQLKEkTDLIVLPEMFTTGFSMNAEALAEPMNGPTLQWMKAQAKKKGAAITGSL 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2475457614  84 VIEKDGTYLNRFLWVTPSGKCSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07575    81 IIKEGGKYYNRLYFVTPDGEVYHYDKRHLFRMAGEHKVYTAGNERVIVEYKGWKILLQVCYDLRF 145
 
Name Accession Description Interval E-value
Xc-1258_like cd07575
Xanthomonas campestris XC1258 and related proteins, members of the nitrilase superfamily ...
 5-148 2.36e-69

Xanthomonas campestris XC1258 and related proteins, members of the nitrilase superfamily (putative class 13 nitrilases); Uncharacterized subgroup belonging to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup either represents a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. XC1258 is a homotetramer.


Pssm-ID: 143599  Cd Length: 252  Bit Score: 210.09  E-value: 2.36e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   5 LQITALQIPLFCEAPALNRKSIDENLTKLHG-TDILLLPEMFTSGFTMNPSTVAESMDGPTIKWMKSWAYSLDAAVGGSI 83
Cdd:cd07575     1 LKIALIQTDLVWEDPEANLAHFEEKIEQLKEkTDLIVLPEMFTTGFSMNAEALAEPMNGPTLQWMKAQAKKKGAAITGSL 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2475457614  84 VIEKDGTYLNRFLWVTPSGKCSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07575    81 IIKEGGKYYNRLYFVTPDGEVYHYDKRHLFRMAGEHKVYTAGNERVIVEYKGWKILLQVCYDLRF 145
PRK10438 PRK10438
C-N hydrolase family amidase; Provisional
 5-148 2.92e-39

C-N hydrolase family amidase; Provisional


Pssm-ID: 182461  Cd Length: 256  Bit Score: 133.33  E-value: 2.92e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   5 LQITALQIPL-FCEAPAlNRKSIDENLTKLHGTDILLLPEMFTSGFTMNPStvAESMDGPT-IKWMKSWAYSLDAAVGGS 82
Cdd:PRK10438    4 LKITLLQQPLvWMDGPA-NLRHFDRQLEGITGRDVIVLPEMFTTGFAMEAA--ASSLPQDDvVAWMTAKAQQTNALIAGS 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2475457614  83 IVIEKDGTYLNRFLWVTPSGKCSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:PRK10438   81 VALQTESGAVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRF 146
Nit2 COG0388
Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];
4-148 4.60e-26

Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];


Pssm-ID: 440157 [Multi-domain]  Cd Length: 264  Bit Score: 99.17  E-value: 4.60e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   4 KLQITALQIPLFCEAPALNRKSIDENLTKLH--GTDILLLPEMFTSGFTMNPSTV---AESMDGPTIKWMKSWAYSLDAA 78
Cdd:COG0388     1 TMRIALAQLNPTVGDIEANLAKIEELIREAAaqGADLVVFPELFLTGYPPEDDDLlelAEPLDGPALAALAELARELGIA 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2475457614  79 VGGSIVIEK-DGTYLNRFLWVTPSGKC-SFYDKHHTF--KLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:COG0388    81 VVVGLPERDeGGRLYNTALVIDPDGEIlGRYRKIHLPnyGVFDEKRYFTPGDELVVFDTDGGRIGVLICYDLWF 154
CN_hydrolase pfam00795
Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. ...
 34-145 6.77e-08

Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1, Aliphatic amidase EC:3.5.1.4, Biotidinase EC:3.5.1.12, Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad, and are multimeric alpha-beta-beta-alpha sandwich proteins.


Pssm-ID: 425873 [Multi-domain]  Cd Length: 257  Bit Score: 50.05  E-value: 6.77e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  34 HGTDILLLPEMFTSGFTMN--PSTVAESMDGPTIKWMKSWAYSLDAAVGGSIV--IEKDGTYLNRFLWVTPSGKCSF-YD 108
Cdd:pfam00795  31 YGADLIVLPELFITGYPCWahFLEAAEVGDGETLAGLAALARKNGIAIVIGLIerWLTGGRLYNTAVLLDPDGKLVGkYR 110

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2475457614 109 KHHTFKLAG-----EYKVYQRGNDDGIIIFKGWKICLRICYD 145
Cdd:pfam00795 111 KLHLFPEPRppgfrERVLFEPGDGGTVFDTPLGKIGAAICYE 152
 
Name Accession Description Interval E-value
Xc-1258_like cd07575
Xanthomonas campestris XC1258 and related proteins, members of the nitrilase superfamily ...
 5-148 2.36e-69

Xanthomonas campestris XC1258 and related proteins, members of the nitrilase superfamily (putative class 13 nitrilases); Uncharacterized subgroup belonging to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup either represents a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. XC1258 is a homotetramer.


Pssm-ID: 143599  Cd Length: 252  Bit Score: 210.09  E-value: 2.36e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   5 LQITALQIPLFCEAPALNRKSIDENLTKLHG-TDILLLPEMFTSGFTMNPSTVAESMDGPTIKWMKSWAYSLDAAVGGSI 83
Cdd:cd07575     1 LKIALIQTDLVWEDPEANLAHFEEKIEQLKEkTDLIVLPEMFTTGFSMNAEALAEPMNGPTLQWMKAQAKKKGAAITGSL 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2475457614  84 VIEKDGTYLNRFLWVTPSGKCSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07575    81 IIKEGGKYYNRLYFVTPDGEVYHYDKRHLFRMAGEHKVYTAGNERVIVEYKGWKILLQVCYDLRF 145
PRK10438 PRK10438
C-N hydrolase family amidase; Provisional
 5-148 2.92e-39

C-N hydrolase family amidase; Provisional


Pssm-ID: 182461  Cd Length: 256  Bit Score: 133.33  E-value: 2.92e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   5 LQITALQIPL-FCEAPAlNRKSIDENLTKLHGTDILLLPEMFTSGFTMNPStvAESMDGPT-IKWMKSWAYSLDAAVGGS 82
Cdd:PRK10438    4 LKITLLQQPLvWMDGPA-NLRHFDRQLEGITGRDVIVLPEMFTTGFAMEAA--ASSLPQDDvVAWMTAKAQQTNALIAGS 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2475457614  83 IVIEKDGTYLNRFLWVTPSGKCSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:PRK10438   81 VALQTESGAVNRFLLVEPGGTVHFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRF 146
nitrilase_5 cd07583
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 7-148 2.14e-26

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143607  Cd Length: 253  Bit Score: 99.92  E-value: 2.14e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   7 ITALQIPLFCEAPALNRKSIDENLTKL--HGTDILLLPEMFTSGFTMN-PSTVAESMDGPTIKWMKSWAYSLDAA-VGGS 82
Cdd:cd07583     2 IALIQLDIVWGDPEANIERVESLIEEAaaAGADLIVLPEMWNTGYFLDdLYELADEDGGETVSFLSELAKKHGVNiVAGS 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2475457614  83 IVIEKDGTYLNRFLWVTPSGKC-SFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07583    82 VAEKEGGKLYNTAYVIDPDGELiATYRKIHLFGLMGEDKYLTAGDELEVFELDGGKVGLFICYDLRF 148
Nit2 COG0388
Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];
4-148 4.60e-26

Omega-amidase YafV/Nit2, hydrolyzes alpha-ketoglutaramate [Energy production and conversion];


Pssm-ID: 440157 [Multi-domain]  Cd Length: 264  Bit Score: 99.17  E-value: 4.60e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   4 KLQITALQIPLFCEAPALNRKSIDENLTKLH--GTDILLLPEMFTSGFTMNPSTV---AESMDGPTIKWMKSWAYSLDAA 78
Cdd:COG0388     1 TMRIALAQLNPTVGDIEANLAKIEELIREAAaqGADLVVFPELFLTGYPPEDDDLlelAEPLDGPALAALAELARELGIA 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2475457614  79 VGGSIVIEK-DGTYLNRFLWVTPSGKC-SFYDKHHTF--KLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:COG0388    81 VVVGLPERDeGGRLYNTALVIDPDGEIlGRYRKIHLPnyGVFDEKRYFTPGDELVVFDTDGGRIGVLICYDLWF 154
nitrilase cd07197
Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing ...
 7-148 1.12e-22

Nitrilase superfamily, including nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes; This superfamily (also known as the C-N hydrolase superfamily) contains hydrolases that break carbon-nitrogen bonds; it includes nitrilases, cyanide dihydratases, aliphatic amidases, N-terminal amidases, beta-ureidopropionases, biotinidases, pantotheinase, N-carbamyl-D-amino acid amidohydrolases, the glutaminase domain of glutamine-dependent NAD+ synthetase, apolipoprotein N-acyltransferases, and N-carbamoylputrescine amidohydrolases, among others. These enzymes depend on a Glu-Lys-Cys catalytic triad, and work through a thiol acylenzyme intermediate. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer. These oligomers include dimers, tetramers, hexamers, octamers, tetradecamers, octadecamers, as well as variable length helical arrangements and homo-oligomeric spirals. These proteins have roles in vitamin and co-enzyme metabolism, in detoxifying small molecules, in the synthesis of signaling molecules, and in the post-translational modification of proteins. They are used industrially, as biocatalysts in the fine chemical and pharmaceutical industry, in cyanide remediation, and in the treatment of toxic effluent. This superfamily has been classified previously in the literature, based on global and structure-based sequence analysis, into thirteen different enzyme classes (referred to as 1-13). This hierarchy includes those thirteen classes and a few additional subfamilies. A putative distant relative, the plasmid-borne TraB family, has not been included in the hierarchy.


Pssm-ID: 143587 [Multi-domain]  Cd Length: 253  Bit Score: 90.08  E-value: 1.12e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   7 ITALQIPLFCEAPALNRKSIDE--NLTKLHGTDILLLPEMFTSGF----TMNPSTVAESMDGPTIKWMKSWAYSLDAAVG 80
Cdd:cd07197     1 IAAVQLAPKIGDVEANLAKALRliKEAAEQGADLIVLPELFLTGYsfesAKEDLDLAEELDGPTLEALAELAKELGIYIV 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2475457614  81 GSIVIEKDGTYLNRFLWVTPSGKCSF-YDKHHTFKLaGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07197    81 AGIAEKDGDKLYNTAVVIDPDGEIIGkYRKIHLFDF-GERRYFSPGDEFPVFDTPGGKIGLLICYDLRF 148
Ph0642_like cd07577
Pyrococcus horikoshii Ph0642 and related proteins, members of the nitrilase superfamily ...
 13-148 2.28e-12

Pyrococcus horikoshii Ph0642 and related proteins, members of the nitrilase superfamily (putative class 13 nitrilases); Uncharacterized subgroup of the nitrilase superfamily. This superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. Pyrococcus horikoshii Ph0642 is a hypothetical protein belonging to this subgroup. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). This subgroup was classified as belonging to class 13, which represents proteins that at the time were difficult to place in a distinct similarity group. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143601  Cd Length: 259  Bit Score: 62.70  E-value: 2.28e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  13 PLFCEaPALNRKSIdENLTKLHGTDILLLPEMFTSGFTMNPS----TVAESM-DGPTIKWMKSWAYSLDAA-VGGsiVIE 86
Cdd:cd07577     9 PKFGE-VEKNLKKV-ESLIKGVEADLIVLPELFNTGYAFTSKeevaSLAESIpDGPTTRFLQELARETGAYiVAG--LPE 84

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2475457614  87 KDGTYL-NRFLWVTPSGKCSFYDKHHTFKlaGEYKVYQRGnDDGIIIFK--GWKICLRICYDFAF 148
Cdd:cd07577    85 RDGDKFyNSAVVVGPEGYIGIYRKTHLFY--EEKLFFEPG-DTGFRVFDigDIRIGVMICFDWYF 146
R-amidase_like cd07576
Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases); ...
 6-148 2.52e-10

Pseudomonas sp. MCI3434 R-amidase and related proteins (putative class 13 nitrilases); Pseudomonas sp. MCI3434 R-amidase hydrolyzes (R,S)-piperazine-2-tert-butylcarboxamide to form (R)-piperazine-2-carboxylic acid. It does so with strict R-stereoselectively. Its preferred substrates are carboxamide compounds which have the amino or imino group connected to their beta- or gamma-carbon. This subgroup belongs to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), class 13 represents proteins that at the time were difficult to place in a distinct similarity group. It has been suggested that this subgroup represents a new class. Members of the nitrilase superfamily generally form homomeric complexes, the basic building block of which is a homodimer. Native R-amidase however appears to be a monomer.


Pssm-ID: 143600  Cd Length: 254  Bit Score: 56.82  E-value: 2.52e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   6 QITALQIPLFCEAPALNRKSIDENLTKL--HGTDILLLPEMFTSGFTMNPSTV--AESMDGPtikWMKSWAySLDAAVGG 81
Cdd:cd07576     1 RLALYQGPARDGDVAANLARLDEAAARAaaAGADLLVFPELFLTGYNIGDAVArlAEPADGP---ALQALR-AIARRHGI 76

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2475457614  82 SIVI---EKDG-TYLNRFLWVTPSGKC-SFYDKHHTFklaGEY--KVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07576    77 AIVVgypERAGgAVYNAAVLIDEDGTVlANYRKTHLF---GDSerAAFTPGDRFPVVELRGLRVGLLICYDVEF 147
nit cd07572
Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases); ...
 35-148 2.98e-09

Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases); This subgroup includes mammalian Nit1 and Nit2, the Nit1-like domain of the invertebrate NitFhit, and various uncharacterized bacterial and archaeal Nit-like proteins. Nit1 and Nit2 are candidate tumor suppressor proteins. In NitFhit, the Nit1-like domain is encoded as a fusion protein with the non-homologous tumor suppressor, fragile histidine triad (Fhit). Mammalian Nit1 and Fhit may affect distinct signal pathways, and both may participate in DNA damage-induced apoptosis. Nit1 is a negative regulator in T cells. Overexpression of Nit2 in HeLa cells leads to a suppression of cell growth through cell cycle arrest in G2. These Nit proteins and the Nit1-like domain of NitFhit belong to a larger nitrilase superfamily comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 10.


Pssm-ID: 143596  Cd Length: 265  Bit Score: 53.97  E-value: 2.98e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  35 GTDILLLPEMFTSgFTMNP----STVAESMDGPTIKWMKSWAYSLDAA-VGGSIVI--EKDGTYLNRFLWVTPSGKC-SF 106
Cdd:cd07572    31 GAKLVVLPECFNY-PGGTDafklALAEEEGDGPTLQALSELAKEHGIWlVGGSIPErdDDDGKVYNTSLVFDPDGELvAR 109

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2475457614 107 YDKHHTF--KLAG-----EYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07572   110 YRKIHLFdvDVPGgisyrESDTLTPGDEVVVVDTPFGKIGLGICYDLRF 158
nitrilase_7 cd07585
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 35-145 8.57e-09

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143609  Cd Length: 261  Bit Score: 52.70  E-value: 8.57e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  35 GTDILLLPEMFTSGFTM--NPSTVAESMDGPTIKWMKSWAYSLDAAVGGSIVIEKDGTYLNRFLWVTPSGKCSFYDKHHT 112
Cdd:cd07585    32 GAELVCFPEMCITGYTHvrALSREAEVPDGPSTQALSDLARRYGLTILAGLIEKAGDRPYNTYLVCLPDGLVHRYRKLHL 111

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 2475457614 113 FKLagEYKVYQRGndDGIIIF--KGWKICLRICYD 145
Cdd:cd07585   112 FRR--EHPYIAAG--DEYPVFatPGVRFGILICYD 142
CN_hydrolase pfam00795
Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. ...
 34-145 6.77e-08

Carbon-nitrogen hydrolase; This family contains hydrolases that break carbon-nitrogen bonds. The family includes: Nitrilase EC:3.5.5.1, Aliphatic amidase EC:3.5.1.4, Biotidinase EC:3.5.1.12, Beta-ureidopropionase EC:3.5.1.6. Nitrilase-related proteins generally have a conserved E-K-C catalytic triad, and are multimeric alpha-beta-beta-alpha sandwich proteins.


Pssm-ID: 425873 [Multi-domain]  Cd Length: 257  Bit Score: 50.05  E-value: 6.77e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  34 HGTDILLLPEMFTSGFTMN--PSTVAESMDGPTIKWMKSWAYSLDAAVGGSIV--IEKDGTYLNRFLWVTPSGKCSF-YD 108
Cdd:pfam00795  31 YGADLIVLPELFITGYPCWahFLEAAEVGDGETLAGLAALARKNGIAIVIGLIerWLTGGRLYNTAVLLDPDGKLVGkYR 110

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2475457614 109 KHHTFKLAG-----EYKVYQRGNDDGIIIFKGWKICLRICYD 145
Cdd:pfam00795 111 KLHLFPEPRppgfrERVLFEPGDGGTVFDTPLGKIGAAICYE 152
nitrilase_3 cd07581
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 19-148 2.69e-07

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143605  Cd Length: 255  Bit Score: 48.34  E-value: 2.69e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  19 PALNRKSIDENLTKLH--GTDILLLPE--MFTSGFT-MNPSTVAESMDGPTIKWMKSWAYSLD-AAVGGSIVIEKDGTYL 92
Cdd:cd07581    12 KEENLEKVRRLLAEAAaaGADLVVFPEytMARFGDGlDDYARVAEPLDGPFVSALARLARELGiTVVAGMFEPAGDGRVY 91

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2475457614  93 NRFLWVTPSGK-CSFYDKHHTFKLAG--EYKVYQRGNDD--GIIIFKGWKICLRICYDFAF 148
Cdd:cd07581    92 NTLVVVGPDGEiIAVYRKIHLYDAFGfrESDTVAPGDELppVVFVVGGVKVGLATCYDLRF 152
nitrilase_2 cd07580
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 6-148 4.20e-06

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143604  Cd Length: 268  Bit Score: 45.03  E-value: 4.20e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614   6 QITALQIPLFCEAPALNRKSIDENLTKL--HGTDILLLPEMFTSGFTMNP-----STVAESMDGPTIKWMKSWAYSLDA- 77
Cdd:cd07580     1 RVACVQFDPRVGDLDANLARSIELIREAadAGANLVVLPELANTGYVFESrdeafALAEEVPDGASTRAWAELAAELGLy 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2475457614  78 AVGGsiVIEKDGTYL-NRFLWVTPSGKCSFYDKHHtfkLAGEYKVYQRGNDDGIIIFK---GwKICLRICYDFAF 148
Cdd:cd07580    81 IVAG--FAERDGDRLyNSAVLVGPDGVIGTYRKAH---LWNEEKLLFEPGDLGLPVFDtpfG-RIGVAICYDGWF 149
nitrilase_6 cd07584
Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The ...
 34-148 9.08e-06

Uncharacterized subgroup of the nitrilase superfamily (putative class 13 nitrilases); The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13). Class 13 represents proteins that at the time were difficult to place in a distinct similarity group; this subgroup represents either a new class or one that was included previously in class 13. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143608  Cd Length: 258  Bit Score: 43.90  E-value: 9.08e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  34 HGTDILLLPEMFTSGFTMNPST-----VAESMDGPTIKWMKSWAYSLdaavGGSIV---IEKDGT----YLNRFLWvTPS 101
Cdd:cd07584    31 EGADLICFPELATTGYRPDLLGpklweLSEPIDGPTVRLFSELAKEL----GVYIVcgfVEKGGVpgkvYNSAVVI-DPE 105

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2475457614 102 GKC-SFYDKHHTFKLagEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07584   106 GESlGVYRKIHLWGL--EKQYFREGEQYPVFDTPFGKIGVMICYDMGF 151
PRK13981 PRK13981
NAD synthetase; Provisional
 35-145 3.07e-03

NAD synthetase; Provisional


Pssm-ID: 237577 [Multi-domain]  Cd Length: 540  Bit Score: 37.06  E-value: 3.07e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  35 GTDILLLPEMFTSGF-----TMNPSTV-----------AESMDGPTIkwmkswaysldaaVGGSIVIEKDGTYlNRfLWV 98
Cdd:PRK13981   33 GADLLLFPELFLSGYppedlLLRPAFLaaceaalerlaAATAGGPAV-------------LVGHPWREGGKLY-NA-AAL 97

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 2475457614  99 TPSGKC-SFYDKHH-----TFKlagEYKVYQRGNDDGIIIFKGWKICLRICYD 145
Cdd:PRK13981   98 LDGGEVlATYRKQDlpnygVFD---EKRYFAPGPEPGVVELKGVRIGVPICED 147
nitrilase_Rim1_like cd07574
Uncharacterized subgroup of the nitrilase superfamily; some members of this subgroup have an ...
 35-148 4.46e-03

Uncharacterized subgroup of the nitrilase superfamily; some members of this subgroup have an N-terminal RimI domain (class 12 nitrilases); Some members of this subgroup are implicated in post-translational modification, as they contain an N-terminal GCN5-related N-acetyltransferase (GNAT) protein RimI family domain. The nitrilase superfamily is comprised of nitrile- or amide-hydrolyzing enzymes and amide-condensing enzymes, which depend on a Glu-Lys-Cys catalytic triad. This superfamily has been classified in the literature based on global and structure based sequence analysis into thirteen different enzyme classes (referred to as 1-13), this subgroup corresponds to class 12. Members of this superfamily generally form homomeric complexes, the basic building block of which is a homodimer.


Pssm-ID: 143598  Cd Length: 280  Bit Score: 36.41  E-value: 4.46e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2475457614  35 GTDILLLPEMFT----SGFTMNPSTVAESMD------GPTIKWMKSWAYSLDAA-VGGSIVIEKDGTYLNRFLWVTPSGK 103
Cdd:cd07574    34 GADLLVFPEYFTmellSLLPEAIDGLDEAIRalaaltPDYVALFSELARKYGINiIAGSMPVREDGRLYNRAYLFGPDGT 113

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2475457614 104 CSFYDKHHTFKLAGEYKVYQRGNDDGIIIFKGWKICLRICYDFAF 148
Cdd:cd07574   114 IGHQDKLHMTPFEREEWGISGGDKLKVFDTDLGKIGILICYDSEF 158
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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