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Conserved domains on  [gi|800613164|emb|CFH60780|]
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aminoglycoside 2'-N-acetyltransferase [Mycobacterium tuberculosis]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 1006192)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

EC:  2.3.1.-
Gene Ontology:  GO:0008080
PubMed:  10940244|9175471
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Eis super family cl34773
Predicted acetyltransferase [General function prediction only];
16-108 3.04e-09

Predicted acetyltransferase [General function prediction only];


The actual alignment was detected with superfamily member COG4552:

Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 54.90  E-value: 3.04e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  16 LDSETRQDIRQMVTGAFAGDFTETDWEHTLGGMHA----LIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAVRADWR 91
Cdd:COG4552    6 LTEDDLDAFARLLAYAFGPEPDDEELEAYRPLLEPgrvlGVFDDGELVGTLALYPFTLNVGGARVPMAGITGVAVAPEHR 85

                         90
                 ....*....|....*..
gi 800613164  92 GQRLVSALLDAVEQVMR 108
Cdd:COG4552   86 RRGVARALLREALAELR 102
 
Name Accession Description Interval E-value
Eis COG4552
Predicted acetyltransferase [General function prediction only];
16-108 3.04e-09

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 54.90  E-value: 3.04e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  16 LDSETRQDIRQMVTGAFAGDFTETDWEHTLGGMHA----LIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAVRADWR 91
Cdd:COG4552    6 LTEDDLDAFARLLAYAFGPEPDDEELEAYRPLLEPgrvlGVFDDGELVGTLALYPFTLNVGGARVPMAGITGVAVAPEHR 85

                         90
                 ....*....|....*..
gi 800613164  92 GQRLVSALLDAVEQVMR 108
Cdd:COG4552   86 RRGVARALLREALAELR 102
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 16-131 1.57e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 50.65  E-value: 1.57e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164   16 LDSETRQDIRQMVTGAFAGDFTETDWE---------HTLGgmhalIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAV 86
Cdd:pfam13527   4 LTEDEFDEVLRLLEYAFQDEDSPELREyfrplleegRVLG-----AFDDGELVSTLALYPFELNVPGKTLPAAGITGVAT 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 800613164   87 RADWRGQRLVSALLDAVEQVMRGAYQ-LGALSSSARArrLYASRGW 131
Cdd:pfam13527  79 YPEYRGRGVMSRLLRRSLEEMRERGVpLSFLYPSSYP--IYRRFGY 122
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-108 4.94e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.87  E-value: 4.94e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 800613164  51 LIWHHGAIIAHAaviqrRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMR 108
Cdd:cd04301    3 VAEDDGEIVGFA-----SLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEAR 55
 
Name Accession Description Interval E-value
Eis COG4552
Predicted acetyltransferase [General function prediction only];
16-108 3.04e-09

Predicted acetyltransferase [General function prediction only];


Pssm-ID: 443616 [Multi-domain]  Cd Length: 393  Bit Score: 54.90  E-value: 3.04e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  16 LDSETRQDIRQMVTGAFAGDFTETDWEHTLGGMHA----LIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAVRADWR 91
Cdd:COG4552    6 LTEDDLDAFARLLAYAFGPEPDDEELEAYRPLLEPgrvlGVFDDGELVGTLALYPFTLNVGGARVPMAGITGVAVAPEHR 85

                         90
                 ....*....|....*..
gi 800613164  92 GQRLVSALLDAVEQVMR 108
Cdd:COG4552   86 RRGVARALLREALAELR 102
Acetyltransf_9 pfam13527
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 16-131 1.57e-08

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 404421 [Multi-domain]  Cd Length: 124  Bit Score: 50.65  E-value: 1.57e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164   16 LDSETRQDIRQMVTGAFAGDFTETDWE---------HTLGgmhalIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAV 86
Cdd:pfam13527   4 LTEDEFDEVLRLLEYAFQDEDSPELREyfrplleegRVLG-----AFDDGELVSTLALYPFELNVPGKTLPAAGITGVAT 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 800613164   87 RADWRGQRLVSALLDAVEQVMRGAYQ-LGALSSSARArrLYASRGW 131
Cdd:pfam13527  79 YPEYRGRGVMSRLLRRSLEEMRERGVpLSFLYPSSYP--IYRRFGY 122
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 40-131 1.94e-08

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 50.21  E-value: 1.94e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164   40 DWEHTLGGMHALIWHHGAIIAHAaviqrRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMRG----AYQLGA 115
Cdd:pfam00583  26 DWDEDASEGFFVAEEDGELVGFA-----SLSIIDDEPPVGEIEGLAVAPEYRGKGIGTALLQALLEWARErgceRIFLEV 100

                          90
                  ....*....|....*.
gi 800613164  116 LSSSARARRLYASRGW 131
Cdd:pfam00583 101 AADNLAAIALYEKLGF 116
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
9-133 6.25e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 46.62  E-value: 6.25e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164   9 RLVHTADLDsetrqDIRQMVTGAFAGDfTETDWEHTL--GGMHALIW---HHGAIIAHAAVIQRRLIYRGNALrcgYVEG 83
Cdd:COG3153    2 RPATPEDAE-----AIAALLRAAFGPG-REAELVDRLreDPAAGLSLvaeDDGEIVGHVALSPVDIDGEGPAL---LLGP 72

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 800613164  84 VAVRADWRGQRLVSALLDAVEQVMRGA-YQLGALSSSARARRLYASRGWLP 133
Cdd:COG3153   73 LAVDPEYRGQGIGRALMRAALEAARERgARAVVLLGDPSLLPFYERFGFRP 123
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 69-131 1.46e-06

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 44.65  E-value: 1.46e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 800613164  69 LIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMR--GAYQ--LGALSSSARARRLYASRGW 131
Cdd:COG0456    4 LLGLVDGGDEAEIEDLAVDPEYRGRGIGRALLEAALERARerGARRlrLEVREDNEAAIALYEKLGF 70
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
49-131 1.30e-05

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 42.86  E-value: 1.30e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  49 HALIWHHGAIIAHAaviqrRLIYRGNALrcGYVEGVAVRADWRGQRLVSALLDAVEQVmrgAYQLGA----LSSSARARR 124
Cdd:COG2153   36 HLLAYDDGELVATA-----RLLPPGDGE--AKIGRVAVLPEYRGQGLGRALMEAAIEE---ARERGArrivLSAQAHAVG 105


                 ....*..
gi 800613164 125 LYASRGW 131
Cdd:COG2153  106 FYEKLGF 112
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 51-131 3.18e-05

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 41.52  E-value: 3.18e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  51 LIWHHGAIIAHAAVIQRrliyrgnALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMR--GAYQLGALSSSArARRLYAS 128
Cdd:COG1246   32 VAEEDGEIVGCAALHPL-------DEDLAELRSLAVHPDYRGRGIGRRLLEALLAEARelGLKRLFLLTTSA-AIHFYEK 103


                 ...
gi 800613164 129 RGW 131
Cdd:COG1246  104 LGF 106
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 51-131 1.92e-04

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 38.59  E-value: 1.92e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164   51 LIWHHGAIIAHAAVIQRRLIYRGNALRcgyvegVAVRADWRGQRLVSALLDAVEQVMR-GAYQLGALSSSARARRLYASR 129
Cdd:pfam13508   7 VAEDDGKIVGFAALLPLDDEGALAELR------LAVHPEYRGQGIGRALLEAAEAAAKeGGIKLLELETTNRAAAFYEKL 80


                  ..
gi 800613164  130 GW 131
Cdd:pfam13508  81 GF 82
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 79-131 2.20e-04

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 39.27  E-value: 2.20e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 800613164  79 GYVEGVAVRADWRGQRLVSALLDAVEQVMR----GAYQLGALSSSARARRLYASRGW 131
Cdd:COG0454   59 LELKRLYVLPEYRGKGIGKALLEALLEWARergcTALELDTLDGNPAAIRFYERLGF 115
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 51-108 4.94e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 36.87  E-value: 4.94e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 800613164  51 LIWHHGAIIAHAaviqrRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMR 108
Cdd:cd04301    3 VAEDDGEIVGFA-----SLSPDGSGGDTAYIGDLAVLPEYRGKGIGSALLEAAEEEAR 55
COG3393 COG3393
Predicted acetyltransferase, GNAT family [General function prediction only];
77-133 6.33e-04

Predicted acetyltransferase, GNAT family [General function prediction only];


Pssm-ID: 442620 [Multi-domain]  Cd Length: 86  Bit Score: 37.20  E-value: 6.33e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 800613164  77 RCGYVEGVAVRADWRGQRLVSALLDAVEQVMRGAY----QLGALSSSARARRLYASRGWLP 133
Cdd:COG3393   14 GVAEISGVYTHPEYRGRGLASALVAALAREALARGartpFLYVDADNPAARRLYERLGFRP 74
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
42-135 8.40e-04

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 38.05  E-value: 8.40e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 800613164  42 EHTLGGMHALI-WHHGAIIAHAAVIQRRliyRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMRGA----YQLGAL 116
Cdd:COG1247   46 AILAPGRPVLVaEEDGEVVGFASLGPFR---PRPAYRGTAEESIYVDPDARGRGIGRALLEALIERARARgyrrLVAVVL 122

                         90
                 ....*....|....*....
gi 800613164 117 SSSARARRLYASRGWLPWH 135
Cdd:COG1247  123 ADNEASIALYEKLGFEEVG 141
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 61-131 1.90e-03

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 36.48  E-value: 1.90e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 800613164   61 HAAVIQRRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQ-VMRGAYQLGALS--SSARARRLYASRGW 131
Cdd:pfam13673  34 FVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAVEDyAEKDGIKLSELTvnASPYAVPFYEKLGF 107
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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