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Conserved domains on  [gi|70886968|gb|EAN99734|]
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MP44, putative [Trypanosoma cruzi]

Protein Classification

ribonuclease III domain-containing protein( domain architecture ID 10457705)

ribonuclease III domain-containing protein similar to Mycoplasmoides pneumoniae ribonuclease 3 that digests double-stranded RNA

CATH:  1.10.1520.10
Gene Ontology:  GO:0006396|GO:0004525
PubMed:  11809414|24124076|17194582
SCOP:  4002876

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ZF_RNaseIII_KREN_KREPB-like super family cl49632
C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) ...
 20-277 1.84e-138

C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) proteins KREN1-3, KREPB4-KREPB10, and related proteins; KREN1-3 (KREPB1-3) and KREPB4-KREPB10 are components of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastid species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing.


The actual alignment was detected with superfamily member cd23725:

Pssm-ID: 483972  Cd Length: 259  Bit Score: 394.89  E-value: 1.84e-138
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  20 LTGRRVEDWEAHNRTFEMRVfeLLKNRLASPTEPLPANQVYVTIANPCRLIDEFNFAKTTRRMDRLRISLGFLRRHNVLL 99
Cdd:cd23725   4 IPGKCVVDWESTLLNFDMRI--LLIQRLCSPTKPLPIKRVYDELANPCRLVEEFDFSKTSRRLARLHLALSFLRRHNVLL 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 100 HGLLFYVKETKTWNTTAGFGRLALFGESLLRHEVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSLVGAKP 179
Cdd:cd23725  82 HSLLFYVEETQTWNTTPEFDRLALYGESVLRTEVRTRLLRLFPDMPASEYSALTSSILSEEALSELFDRLEMQALVGARP 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 180 EGKDRDWSLTKEQRSSMLCAIVGEMSWFSSRTKATDRTHNNALFPPSDALILHVLCCHVLESLPAELIYTVVEPRVHRLK 259
Cdd:cd23725 162 RGKRRHHSLTPAQKRQMLCAVVGEMHWFVARTKATDRTHNNALFPPSDVLILHVLCAHLLESVPAELIYNVLEPIVKDIK 241

                       250
                ....*....|....*...
gi 70886968 260 EVWVNEPMSIPSQMHLKP 277
Cdd:cd23725 242 RVWVNEPMSLPSQLRLVP 259
 
Name Accession Description Interval E-value
RNaseIII_KREPB5 cd23725
Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex ...
 20-277 1.84e-138

Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) protein KREPB5; KREPB5 (MP44) has a divergent RNase III domain and is a component of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing. The C2H2 zinc finger that is present in related KREB proteins is not conserved in this subfamily.


Pssm-ID: 469568  Cd Length: 259  Bit Score: 394.89  E-value: 1.84e-138
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  20 LTGRRVEDWEAHNRTFEMRVfeLLKNRLASPTEPLPANQVYVTIANPCRLIDEFNFAKTTRRMDRLRISLGFLRRHNVLL 99
Cdd:cd23725   4 IPGKCVVDWESTLLNFDMRI--LLIQRLCSPTKPLPIKRVYDELANPCRLVEEFDFSKTSRRLARLHLALSFLRRHNVLL 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 100 HGLLFYVKETKTWNTTAGFGRLALFGESLLRHEVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSLVGAKP 179
Cdd:cd23725  82 HSLLFYVEETQTWNTTPEFDRLALYGESVLRTEVRTRLLRLFPDMPASEYSALTSSILSEEALSELFDRLEMQALVGARP 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 180 EGKDRDWSLTKEQRSSMLCAIVGEMSWFSSRTKATDRTHNNALFPPSDALILHVLCCHVLESLPAELIYTVVEPRVHRLK 259
Cdd:cd23725 162 RGKRRHHSLTPAQKRQMLCAVVGEMHWFVARTKATDRTHNNALFPPSDVLILHVLCAHLLESVPAELIYNVLEPIVKDIK 241

                       250
                ....*....|....*...
gi 70886968 260 EVWVNEPMSIPSQMHLKP 277
Cdd:cd23725 242 RVWVNEPMSLPSQLRLVP 259
 
Name Accession Description Interval E-value
RNaseIII_KREPB5 cd23725
Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex ...
 20-277 1.84e-138

Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) protein KREPB5; KREPB5 (MP44) has a divergent RNase III domain and is a component of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing. The C2H2 zinc finger that is present in related KREB proteins is not conserved in this subfamily.


Pssm-ID: 469568  Cd Length: 259  Bit Score: 394.89  E-value: 1.84e-138
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  20 LTGRRVEDWEAHNRTFEMRVfeLLKNRLASPTEPLPANQVYVTIANPCRLIDEFNFAKTTRRMDRLRISLGFLRRHNVLL 99
Cdd:cd23725   4 IPGKCVVDWESTLLNFDMRI--LLIQRLCSPTKPLPIKRVYDELANPCRLVEEFDFSKTSRRLARLHLALSFLRRHNVLL 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 100 HGLLFYVKETKTWNTTAGFGRLALFGESLLRHEVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSLVGAKP 179
Cdd:cd23725  82 HSLLFYVEETQTWNTTPEFDRLALYGESVLRTEVRTRLLRLFPDMPASEYSALTSSILSEEALSELFDRLEMQALVGARP 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 180 EGKDRDWSLTKEQRSSMLCAIVGEMSWFSSRTKATDRTHNNALFPPSDALILHVLCCHVLESLPAELIYTVVEPRVHRLK 259
Cdd:cd23725 162 RGKRRHHSLTPAQKRQMLCAVVGEMHWFVARTKATDRTHNNALFPPSDVLILHVLCAHLLESVPAELIYNVLEPIVKDIK 241

                       250
                ....*....|....*...
gi 70886968 260 EVWVNEPMSIPSQMHLKP 277
Cdd:cd23725 242 RVWVNEPMSLPSQLRLVP 259
ZF_RNaseIII_KREPB4-5 cd23719
C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) ...
 20-266 2.75e-102

C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) proteins KREPB4-5; KREPB4 (MP46) and KREPB5 (MP44) have divergent RNase III domains and are components of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing.


Pssm-ID: 469562  Cd Length: 261  Bit Score: 303.16  E-value: 2.75e-102
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  20 LTGRRVEDWEAHNRTFEMRVFELLKNRLASPTEPLPANQVYV-TIANPCRLIDEFNFAKTTRRMDRLRISLGFLRRHNVL 98
Cdd:cd23719   4 LCDVSYESWGDHAESTTHIARHAICRTFVSPERHNAVMQQLWkHIRLDFGYVDEVTHKKEDRRRMRLASTMRHLQEKGVL 83

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  99 LHGLLFYVKETK----TWNTTAGFGRLALFGESLLRHEVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSL 174
Cdd:cd23719  84 HHSLPRVTVDAQsevsLTVESDSFVNYMFLGESFARQETLDRVARLMPRAEALELSSIISFVLSKRRLAHFFDILLMRKM 163

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 175 VGAKPEGKD------------RDWSLTKEQRSSMLCAIVGEMswFSSRtkatDRTHNNALFPPSDALILHVLCCHVLESL 242
Cdd:cd23719 164 VLNGDSSDDkasadgdvpptiPRLQQDGKAVILFSCLGELQM--FSRR----DRSHSVATRSAAEQLVLNVLGTHVMENI 237

                       250       260
                ....*....|....*....|....
gi 70886968 243 PAELIYTVVEPRVHRLKEVWVNEP 266
Cdd:cd23719 238 IGELVHEALQTVVEEGTAVWREHC 261
ZF_RNaseIII_KREN_KREPB-like cd23383
C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) ...
 20-263 7.02e-41

C2H2 zinc finger and Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) proteins KREN1-3, KREPB4-KREPB10, and related proteins; KREN1-3 (KREPB1-3) and KREPB4-KREPB10 are components of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastid species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing.


Pssm-ID: 469560  Cd Length: 195  Bit Score: 142.87  E-value: 7.02e-41
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  20 LTGRRVEDWEAHNRTFEMRVFELLKNRLASPTeplpanqvyvtianpcrlidefnfakttrrmdRLRISLGFLRRHNVLL 99
Cdd:cd23383   4 LCNEPVGSWGEHVGSRDHRILEMFYDAIVEYG--------------------------------KLLAALSFLRRSGVLL 51

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 100 HgllfyvketKTWNTTAGFGRLALFGESLLRHEVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSLVGAKP 179
Cdd:cd23383  52 A---------SFHRRRLSGSGLLIKGSNVLPKHLARRLMRLFPGADSGYLSAFTQSIASEYNMETVFDLLGLKRLFPEKS 122

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 180 egkdRDWSLTKEQRSSMLCAIVGEMSWFSSRTKATDrthnnalFPPSDALILHVLCCHVLESLPAELIYTVVEPRVHRLK 259
Cdd:cd23383 123 ----RDPLLSFHRKGDFVRALIGELHWALIRREGIP-------PPPSDSARLRVLAQFVLRSLVAELIYLRLSEYVQRVD 191


                ....
gi 70886968 260 EVWV 263
Cdd:cd23383 192 GVWR 195
ZF_RNaseIII_KREPB4 cd23724
C2H2 zinc finger and Ribonuclease III-like domain of Ribonuclease III-like domain of ...
 70-262 2.65e-19

C2H2 zinc finger and Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) protein KREPB4; KREPB4 (MP46) has a divergent RNase III domain and is a component of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains, lacking the conserved catalytic residues. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing.


Pssm-ID: 469567  Cd Length: 251  Bit Score: 86.37  E-value: 2.65e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  70 IDEFNFAKTTRRMDRLRISLGFLRRHNVLLHGLLFYVKETKTWNTTA-----------------GFGRLALFGESLLRHE 132
Cdd:cd23724  57 VDALTWRKERRRRERLLSTLRHLVEKGVLRDSLPTVSCSAPTREGTDkgrgdaisgasltvvsdGFLNYVLVGEGYARQE 136

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 133 VRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMMKSLVgakpegkdrdwsltkEQRSSMLCAIVGEMSWFSSRtk 212
Cdd:cd23724 137 VLDRVARLMPSAEAMELNAVATYLLSTRQLARIFDELSMEELL---------------DGKAYIMLACLGELQRFHRQ-- 199

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|
gi 70886968 213 atDRTHNNALFPPSDALILHVLCCHVLESLPAELIYTVVEPRVHRLKEVW 262
Cdd:cd23724 200 --DRPRSVASKAAADALVLNVLATHVRENLISELVHEVLQRIVDEGTPVW 247
ZF_RNaseIII_KREPB6 cd23723
C2H2 zinc finger and Ribonuclease III-like domain of Ribonuclease III-like domain of ...
 93-265 2.46e-03

C2H2 zinc finger and Ribonuclease III-like domain of Ribonuclease III-like domain of RNA-editing catalytic complex (RECC) protein KREPB6; KREPB6 (MP49) has a divergent RNase III domain and is a component of the RNA-editing catalytic complex (RECC) involved in U-insertion/deletion mRNA editing during kinetoplast RNA processing. The mitochondrial DNA of Trypanosomatids, known as the kinetoplast DNA (kDNA or mtDNA), consists of a network of dozens of maxicircles and thousands of minicircles concatenated together. Maxicircles are equivalent to other eukaryotic mitochondrial DNAs, while minicircles encode guide RNAs (gRNAs) involved in U-insertion/deletion editing processes exclusive of Trypanosomatids that produce the maturation of the maxicircle-encoded transcripts. Although most gRNAs are encoded by minicircles, varying numbers of maxicircle-encoded gRNAs have been identified in kinetoplastids species. Trypanosoma brucei maxicircles encode 9S and 12S rRNAs, two gRNAs, two ribosomal proteins and 16 subunits of respiratory complexes. 12 of the 18 maxicircle genes are present as cryptogenes whose transcripts require U-insertion/deletion editing, mediated by gRNAs, to restore a protein-coding capacity. The RECC core complex comprises a U-insertion subcomplex (KRET2 TUTase, KREPA1 zinc-finger protein, and KREL2 RNA ligase), a U-deletion subcomplex (KREX2 exonuclease, KREPA2 zinc-finger protein, and KREL1 RNA ligase), and six structural and/or RNA-binding proteins (KREPA3, KREPA4, KREPA5, KREPA6, KREPB4, and KREPB5). This core complex interacts with one of three RNase III family endonucleases (KREN1, KREN2, and KREN3) that may partner with one or more partner protein(s) with divergent RNase III domains, such as KREN1/KREPB8, KREN2/KREPB7, KREN3/KREPB6. KREPB4-KREPB10 have divergent RNase III domains. RECC interacts with two other complexes, the RNA-editing helicase 2 complex (REH2C) and the RNA-editing substrate-binding complex (RESC) to form an assembly (editosome/holoenzyme) that carries out U-insertion/deletion mRNA editing.


Pssm-ID: 469566  Cd Length: 218  Bit Score: 39.00  E-value: 2.46e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968  93 RRHNvlLHGLLFYVKETKTWNTTAGFGRLALFGESLLRH-EVRARTLRLFPSIDSATYASLTASILNEDALCAIFDRLMM 171
Cdd:cd23723  55 RREE--LHACLQHLKECGMLRLDPSLFGGASFHGSLVLFkELFPVLARMFPDAEAKEISALTQMIASTYNLETVFDLCGM 132

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 70886968 172 KSLVgakpegkDRDWSltkeqrsSMLC-AIVGEMSWfSSRTKATDRTHnnALFPPSDAL---ILHVLCCHVLESLPAELI 247
Cdd:cd23723 133 ERLV-------PYSLK-------GAFCrAVLGQLRW-ALEPDSTAPPP--AIAQGSHHKrdpYWTTLAARTARLLLSELI 195

                       170
                ....*....|....*...
gi 70886968 248 YTVVEPRVHRLKEVWVNE 265
Cdd:cd23723 196 FCRVSEYVVRVEGVLRTE 213
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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