NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|575844630|gb|ETY75783|]
View 

pyridoxamine 5'-phosphate oxidase [Lactiplantibacillus fabifermentans T30PCM01]

Protein Classification

pyridoxamine 5'-phosphate oxidase family protein( domain architecture ID 10472314)

pyridoxamine 5'-phosphate oxidase family protein may catalyze an FMN-mediated redox reaction, similar to Aspergillus flavus pyridoxamine 5'-phosphate oxidase family protein ustO that is part of the gene cluster that mediates the biosynthesis of the secondary metabolite ustiloxin B, an antimitotic tetrapeptide

CATH:  2.30.110.10
EC:  1.-.-.-
Gene Ontology:  GO:0010181|GO:0016491
PubMed:  26434506|32951820
SCOP:  4002129

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
YzzA super family cl39142
General stress protein 26 (function unknown) [Function unknown];
1-98 2.84e-09

General stress protein 26 (function unknown) [Function unknown];


The actual alignment was detected with superfamily member COG3871:

Pssm-ID: 443080 [Multi-domain]  Cd Length: 132  Bit Score: 51.86  E-value: 2.84e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 575844630   1 MQHTTRQAALQMIQTAAVFTVATIDASGFPTLVALSPLPTRRSLAqLFFYTSRQTMTAKNIQSCKKATLFCYNLDDYASV 80
Cdd:COG3871    3 DDEELEEKLWELLEDIRTAMLATVDADGRPHSRPMWFQVDVDDGT-LWFFTSRDSAKVRNIRRDPRVSLSFADPGDDRYV 81

                         90       100
                 ....*....|....*....|
gi 575844630  81 MLKGQLRVVgHDE--FAEDW 98
Cdd:COG3871   82 SVEGTAEIV-DDRakIDELW 100
 
Name Accession Description Interval E-value
YzzA COG3871
General stress protein 26 (function unknown) [Function unknown];
1-98 2.84e-09

General stress protein 26 (function unknown) [Function unknown];


Pssm-ID: 443080 [Multi-domain]  Cd Length: 132  Bit Score: 51.86  E-value: 2.84e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 575844630   1 MQHTTRQAALQMIQTAAVFTVATIDASGFPTLVALSPLPTRRSLAqLFFYTSRQTMTAKNIQSCKKATLFCYNLDDYASV 80
Cdd:COG3871    3 DDEELEEKLWELLEDIRTAMLATVDADGRPHSRPMWFQVDVDDGT-LWFFTSRDSAKVRNIRRDPRVSLSFADPGDDRYV 81

                         90       100
                 ....*....|....*....|
gi 575844630  81 MLKGQLRVVgHDE--FAEDW 98
Cdd:COG3871   82 SVEGTAEIV-DDRakIDELW 100
Putative_PNPOx pfam01243
Pyridoxamine 5'-phosphate oxidase; Family of domains with putative PNPOx function. Family ...
 7-89 9.43e-07

Pyridoxamine 5'-phosphate oxidase; Family of domains with putative PNPOx function. Family members were predicted to encode pyridoxamine 5'-phosphate oxidase, based on sequence similarity. However, there is no experimental data to validate the predicted activity and purified proteins, such as Swiss:Q06199 and its paralogs, do not possess this activity, nor do they bind to flavin mononucleotide (FMN). To date, the only time functional oxidase activity has been experimentally demonstrated is when the sequences contain both pfam01243 and pfam10590. Moreover, some of the family members that contain both domains have been shown to be involved in phenazine biosynthesis. While some molecular function has been experimentally validated for the proteins containing both domains, the role performed by each domain on its own is unknown.


Pssm-ID: 426149 [Multi-domain]  Cd Length: 88  Bit Score: 44.16  E-value: 9.43e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 575844630    7 QAALQMIQTAAVFTVATIDASGFP--TLVALSPLPTRRSLaqlFFYTSRQTMTAKNIQSCKKATLFCYNLDDYASVMLKG 84
Cdd:pfam01243   3 EEIREFLAEPNAVVLATVDKDGRPnvRPVGLKYGFDTVGI---LFATNTDSRKARNLEENPRVALLFGDPELRRGVRIEG 79


                  ....*
gi 575844630   85 QLRVV 89
Cdd:pfam01243  80 TAEIV 84
 
Name Accession Description Interval E-value
YzzA COG3871
General stress protein 26 (function unknown) [Function unknown];
1-98 2.84e-09

General stress protein 26 (function unknown) [Function unknown];


Pssm-ID: 443080 [Multi-domain]  Cd Length: 132  Bit Score: 51.86  E-value: 2.84e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 575844630   1 MQHTTRQAALQMIQTAAVFTVATIDASGFPTLVALSPLPTRRSLAqLFFYTSRQTMTAKNIQSCKKATLFCYNLDDYASV 80
Cdd:COG3871    3 DDEELEEKLWELLEDIRTAMLATVDADGRPHSRPMWFQVDVDDGT-LWFFTSRDSAKVRNIRRDPRVSLSFADPGDDRYV 81

                         90       100
                 ....*....|....*....|
gi 575844630  81 MLKGQLRVVgHDE--FAEDW 98
Cdd:COG3871   82 SVEGTAEIV-DDRakIDELW 100
Putative_PNPOx pfam01243
Pyridoxamine 5'-phosphate oxidase; Family of domains with putative PNPOx function. Family ...
 7-89 9.43e-07

Pyridoxamine 5'-phosphate oxidase; Family of domains with putative PNPOx function. Family members were predicted to encode pyridoxamine 5'-phosphate oxidase, based on sequence similarity. However, there is no experimental data to validate the predicted activity and purified proteins, such as Swiss:Q06199 and its paralogs, do not possess this activity, nor do they bind to flavin mononucleotide (FMN). To date, the only time functional oxidase activity has been experimentally demonstrated is when the sequences contain both pfam01243 and pfam10590. Moreover, some of the family members that contain both domains have been shown to be involved in phenazine biosynthesis. While some molecular function has been experimentally validated for the proteins containing both domains, the role performed by each domain on its own is unknown.


Pssm-ID: 426149 [Multi-domain]  Cd Length: 88  Bit Score: 44.16  E-value: 9.43e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 575844630    7 QAALQMIQTAAVFTVATIDASGFP--TLVALSPLPTRRSLaqlFFYTSRQTMTAKNIQSCKKATLFCYNLDDYASVMLKG 84
Cdd:pfam01243   3 EEIREFLAEPNAVVLATVDKDGRPnvRPVGLKYGFDTVGI---LFATNTDSRKARNLEENPRVALLFGDPELRRGVRIEG 79


                  ....*
gi 575844630   85 QLRVV 89
Cdd:pfam01243  80 TAEIV 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH