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Conserved domains on  [gi|2033800011|dbj|GIS66085|]
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MAG: glycine cleavage system H protein [Rhodospirillaceae bacterium]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 5-127 2.62e-63

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 189.18  E-value: 2.62e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:COG0509     8 KYTEDHEWVRVEGDG-----TATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNE 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:COG0509    83 ALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALL 125
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 5-127 2.62e-63

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 189.18  E-value: 2.62e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:COG0509     8 KYTEDHEWVRVEGDG-----TATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNE 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:COG0509    83 ALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALL 125
PRK01202 PRK01202
glycine cleavage system protein GcvH;
5-127 7.58e-62

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 185.36  E-value: 7.58e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGgektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:PRK01202    9 KYTKSHEWVRVEGD------TATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNE 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:PRK01202   83 ALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALI 125
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 5-127 3.73e-48

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 150.56  E-value: 3.73e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:pfam01597   2 YYTKEHEWVKVEGDG-----TATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNE 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:pfam01597  77 KLEDNPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTL 119
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-106 3.66e-46

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 144.60  E-value: 3.66e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   6 YTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININNT 85
Cdd:cd06848     1 YTKDHEWVKVEGDG-----IATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEA 75

                          90       100
                  ....*....|....*....|.
gi 2033800011  86 IIDDPGLINSDPREVGWFFSL 106
Cdd:cd06848    76 LLDNPELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 5-124 2.86e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 130.73  E-value: 2.86e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEegGEKTkaiGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:TIGR00527   7 RYSSEHEWVRVE--GDKT---ATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNT 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADP-SELENLMDEAAYE 124
Cdd:TIGR00527  82 ALEDSPELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYE 122
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 5-127 2.62e-63

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 189.18  E-value: 2.62e-63
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:COG0509     8 KYTEDHEWVRVEGDG-----TATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNE 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:COG0509    83 ALEDDPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALL 125
PRK01202 PRK01202
glycine cleavage system protein GcvH;
5-127 7.58e-62

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 185.36  E-value: 7.58e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGgektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:PRK01202    9 KYTKSHEWVRVEGD------TATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNE 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:PRK01202   83 ALEDSPELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALI 125
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 5-127 3.73e-48

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 150.56  E-value: 3.73e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:pfam01597   2 YYTKEHEWVKVEGDG-----TATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNE 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAYESFV 127
Cdd:pfam01597  77 KLEDNPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTL 119
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 6-106 3.66e-46

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 144.60  E-value: 3.66e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   6 YTKDHEWVRLEEGGektkaIGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININNT 85
Cdd:cd06848     1 YTKDHEWVKVEGDG-----IATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEA 75

                          90       100
                  ....*....|....*....|.
gi 2033800011  86 IIDDPGLINSDPREVGWFFSL 106
Cdd:cd06848    76 LLDNPELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 5-124 2.86e-40

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 130.73  E-value: 2.86e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   5 KYTKDHEWVRLEegGEKTkaiGIVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININN 84
Cdd:TIGR00527   7 RYSSEHEWVRVE--GDKT---ATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNT 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 2033800011  85 TIIDDPGLINSDPREVGWFFSLELADP-SELENLMDEAAYE 124
Cdd:TIGR00527  82 ALEDSPELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYE 122
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 6-123 3.47e-40

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 130.89  E-value: 3.47e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011   6 YTKDHEWVRLEEGGEKTkaigiVGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININNT 85
Cdd:PRK13380   16 TIKNHEWLRLEGDGTVT-----VGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVVEVNEA 90

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 2033800011  86 IIDDPGLINSDPREVGWFFSLELADPSELENLMDEAAY 123
Cdd:PRK13380   91 LEDSPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAA 128
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 28-117 4.33e-19

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 76.41  E-value: 4.33e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2033800011  28 VGITDFAQEQLGDIVFVELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININNTIIDDPGLINSDPREVGWFFSLE 107
Cdd:PRK00624   21 LGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDIQPINNAPESEGWFVVVQ 100

                          90
                  ....*....|
gi 2033800011 108 LADPSELENL 117
Cdd:PRK00624  101 LDEDFDSENL 110
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 27-83 1.92e-08

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 47.82  E-value: 1.92e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2033800011  27 IVGITDFAQeQLGDIVFV-ELPEVGITVQKGDEAATIESVKAASEFFTPVSGKVININ 83
Cdd:cd06663     1 TILIPDLAQ-HLGDGTVVkWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVL 57
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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