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Conserved domains on  [gi|2065857412|gb|KAG7623532|]
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ENTH/VHS [Arabidopsis suecica]

Protein Classification

VHS/ENTH/ANTH domain-containing protein; ENTH domain-containing protein( domain architecture ID 13016533)

VHS (Vps27/Hrs/STAM) /ENTH (Epsin N-Terminal Homology) /ANTH (AP180 N-Terminal Homology) domain-containing protein similar to Homo sapiens ADP-ribosylation factor-binding protein GGA3 that plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes; ENTH (Epsin N-Terminal Homology) domain-containing protein may be involved in clathrin-mediated endocytosis; similar to Saccharomyces cerevisiae epsin-4 that may be involved in the Trans-Golgi Network (TGN)-to-vacuole sorting of Arn1p, a transporter for the uptake of ferrichrome, an important nutritional source of iron

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ANTH_N_AP180_plant cd16987
ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly ...
 37-160 8.64e-54

ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly protein AP180 and similar proteins; This subfamily is composed of plant clathrin coat assembly protein AP180 and other ANTH domain containing proteins that are yet to be characterized. Arabidopsis thaliana AP180 (At-AP180) is a binding partner of plant alphaC-adaptin; it functions as a clathrin assembly protein that promotes the formation of cages with an almost uniform size distribution. In addition to At-AP180, Arabidopsis thaliana contains many ANTH domain containing proteins labelled as putative clathrin assembly proteins included in this subfamily such as At4g02650, At5g10410, At2g25430, and At1g33340, among others. ANTH domains bind both inositol phospholipids and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. The ANTH domain is a unique module whose N-terminal half is structurally similar to the Epsin N-Terminal Homology (ENTH) and Vps27/Hrs/STAM (VHS) domains, containing a superhelix of eight alpha helices. In addition, it contains a coiled-coil C-terminal half with strutural similarity to spectrin repeats. It binds phosphoinositide PtdIns(4,5)P2 at a short conserved motif K[X]9[K/R][H/Y] between helices 1 and 2. This model describes the N-terminal region of ANTH domains of plant clathrin coat assembly protein AP180 and similar proteins.


:

Pssm-ID: 340784  Cd Length: 122  Bit Score: 173.19  E-value: 8.64e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  37 FHLSVLRATTHDpSTPPGNRHLAVILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSV 116
Cdd:cd16987     1 LEVAVVKATSHD-DAPPDEKYVREILSLGSSSRAYASACVSALSRRLNRTRDWVVALKCLMLLHRLLRDGSPILEQELSL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2065857412 117 FPaSGGRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLSTS 160
Cdd:cd16987    80 AP-SGGRNPLNLSDFRDGSSSKSWDFSAFVRAYAAYLDERLIFS 122
 
Name Accession Description Interval E-value
ANTH_N_AP180_plant cd16987
ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly ...
 37-160 8.64e-54

ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly protein AP180 and similar proteins; This subfamily is composed of plant clathrin coat assembly protein AP180 and other ANTH domain containing proteins that are yet to be characterized. Arabidopsis thaliana AP180 (At-AP180) is a binding partner of plant alphaC-adaptin; it functions as a clathrin assembly protein that promotes the formation of cages with an almost uniform size distribution. In addition to At-AP180, Arabidopsis thaliana contains many ANTH domain containing proteins labelled as putative clathrin assembly proteins included in this subfamily such as At4g02650, At5g10410, At2g25430, and At1g33340, among others. ANTH domains bind both inositol phospholipids and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. The ANTH domain is a unique module whose N-terminal half is structurally similar to the Epsin N-Terminal Homology (ENTH) and Vps27/Hrs/STAM (VHS) domains, containing a superhelix of eight alpha helices. In addition, it contains a coiled-coil C-terminal half with strutural similarity to spectrin repeats. It binds phosphoinositide PtdIns(4,5)P2 at a short conserved motif K[X]9[K/R][H/Y] between helices 1 and 2. This model describes the N-terminal region of ANTH domains of plant clathrin coat assembly protein AP180 and similar proteins.


Pssm-ID: 340784  Cd Length: 122  Bit Score: 173.19  E-value: 8.64e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  37 FHLSVLRATTHDpSTPPGNRHLAVILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSV 116
Cdd:cd16987     1 LEVAVVKATSHD-DAPPDEKYVREILSLGSSSRAYASACVSALSRRLNRTRDWVVALKCLMLLHRLLRDGSPILEQELSL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2065857412 117 FPaSGGRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLSTS 160
Cdd:cd16987    80 AP-SGGRNPLNLSDFRDGSSSKSWDFSAFVRAYAAYLDERLIFS 122
ANTH pfam07651
ANTH domain; AP180 is an endocytotic accessory proteins that has been implicated in the ...
 40-202 7.46e-13

ANTH domain; AP180 is an endocytotic accessory proteins that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.


Pssm-ID: 400137  Cd Length: 272  Bit Score: 68.09  E-value: 7.46e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  40 SVLRATTHDpSTPPGNRHLAVILSaGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHivkhgrfILQDQLSVFPA 119
Cdd:pfam07651   5 AVVKATSHD-EAPPKEKHVREILV-GTSSSAKLAALFWALSRRLPLTRSWVVAFKALILVHK-------LLREGHPSVLQ 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412 120 SGGRNYLKLSAFRD-EKSPLMWELSSWVRWYALYLEHLLSTSRIMGFF-----ISSTSSTIHKEEYEEMVSSLTNSDLLR 193
Cdd:pfam07651  76 ELLRARRRISSLLRiSSFSLSWDYGAFIRAYAKYLDERLDFHRKLPRDpgtfeRVEYGSLVAVGDPNERYLTMSMEDLLD 155


                  ....*....
gi 2065857412 194 EIDALVGLL 202
Cdd:pfam07651 156 SIPKLQKLL 164
ENTH smart00273
Epsin N-terminal homology (ENTH) domain;
40-166 2.56e-12

Epsin N-terminal homology (ENTH) domain;


Pssm-ID: 214594  Cd Length: 127  Bit Score: 63.42  E-value: 2.56e-12
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412   40 SVLRATTHDPsTPPGNRHLAVILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHG--RFILQDQLSVF 117
Cdd:smart00273   6 KVRKATNNDE-WGPKGKHLREIIQGTHNEKSSFAEIMAVLWRRLNDTKNWRVVYKALILLHYLLRNGspRVILEALRNRN 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*....
gi 2065857412  118 pasggrNYLKLSAFRDeKSPLMWELSSWVRWYALYLEHLLSTSRIMGFF 166
Cdd:smart00273  85 ------RILNLSDFQD-IDSRGKDQGANIRTYAKYLLERLEDDRRLKEE 126
 
Name Accession Description Interval E-value
ANTH_N_AP180_plant cd16987
ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly ...
 37-160 8.64e-54

ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of plant Clathrin coat assembly protein AP180 and similar proteins; This subfamily is composed of plant clathrin coat assembly protein AP180 and other ANTH domain containing proteins that are yet to be characterized. Arabidopsis thaliana AP180 (At-AP180) is a binding partner of plant alphaC-adaptin; it functions as a clathrin assembly protein that promotes the formation of cages with an almost uniform size distribution. In addition to At-AP180, Arabidopsis thaliana contains many ANTH domain containing proteins labelled as putative clathrin assembly proteins included in this subfamily such as At4g02650, At5g10410, At2g25430, and At1g33340, among others. ANTH domains bind both inositol phospholipids and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. The ANTH domain is a unique module whose N-terminal half is structurally similar to the Epsin N-Terminal Homology (ENTH) and Vps27/Hrs/STAM (VHS) domains, containing a superhelix of eight alpha helices. In addition, it contains a coiled-coil C-terminal half with strutural similarity to spectrin repeats. It binds phosphoinositide PtdIns(4,5)P2 at a short conserved motif K[X]9[K/R][H/Y] between helices 1 and 2. This model describes the N-terminal region of ANTH domains of plant clathrin coat assembly protein AP180 and similar proteins.


Pssm-ID: 340784  Cd Length: 122  Bit Score: 173.19  E-value: 8.64e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  37 FHLSVLRATTHDpSTPPGNRHLAVILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSV 116
Cdd:cd16987     1 LEVAVVKATSHD-DAPPDEKYVREILSLGSSSRAYASACVSALSRRLNRTRDWVVALKCLMLLHRLLRDGSPILEQELSL 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 2065857412 117 FPaSGGRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLSTS 160
Cdd:cd16987    80 AP-SGGRNPLNLSDFRDGSSSKSWDFSAFVRAYAAYLDERLIFS 122
ANTH_N cd03564
ANTH (AP180 N-Terminal Homology) domain family, N-terminal region; The ANTH (AP180 N-Terminal ...
 40-159 1.40e-18

ANTH (AP180 N-Terminal Homology) domain family, N-terminal region; The ANTH (AP180 N-Terminal Homology) domain family is composed of Adaptor Protein 180 (AP180), Clathrin Assembly Lymphoid Myeloid Leukemia protein (CALM), and similar proteins. ANTH domains bind both inositol phospholipids and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ANTH-bearing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that the ANTH domain is a universal component of the machinery for clathrin-mediated membrane budding. The ANTH domain is a unique module whose N-terminal half is structurally similar to the Epsin N-Terminal Homology (ENTH) and Vps27/Hrs/STAM (VHS) domains, containing a superhelix of eight alpha helices. In addition, it contains a coiled-coil C-terminal half with strutural similarity to spectrin repeats. It binds phosphoinositide PtdIns(4,5)P2 at a short conserved motif K[X]9[K/R][H/Y] between helices 1 and 2. This model describes the N-terminal region of ANTH domains.


Pssm-ID: 340767  Cd Length: 120  Bit Score: 80.40  E-value: 1.40e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  40 SVLRATTHDPsTPPGNRHLAVILSA--GTGSRATASSAVESIMERLHTTgDACVALKSLIIIHHIVKHGRFILQDQLSVF 117
Cdd:cd03564     4 AVVKATNHDE-VPPKEKHVRKLLLAtsNGGGRADVAYIVHALAKRLHKK-NWIVVLKTLIVIHRLLREGSPSFLEELLRY 81

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 2065857412 118 PasggRNYLKLSAFRDEKSPLMWELSSWVRWYALYLEHLLST 159
Cdd:cd03564    82 S----GHIFNLSNFKDDSSPEAWDLSAFIRRYARYLEERLEC 119
ANTH pfam07651
ANTH domain; AP180 is an endocytotic accessory proteins that has been implicated in the ...
 40-202 7.46e-13

ANTH domain; AP180 is an endocytotic accessory proteins that has been implicated in the formation of clathrin-coated pits. The domain is involved in phosphatidylinositol 4,5-bisphosphate binding and is a universal adaptor for nucleation of clathrin coats.


Pssm-ID: 400137  Cd Length: 272  Bit Score: 68.09  E-value: 7.46e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  40 SVLRATTHDpSTPPGNRHLAVILSaGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHivkhgrfILQDQLSVFPA 119
Cdd:pfam07651   5 AVVKATSHD-EAPPKEKHVREILV-GTSSSAKLAALFWALSRRLPLTRSWVVAFKALILVHK-------LLREGHPSVLQ 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412 120 SGGRNYLKLSAFRD-EKSPLMWELSSWVRWYALYLEHLLSTSRIMGFF-----ISSTSSTIHKEEYEEMVSSLTNSDLLR 193
Cdd:pfam07651  76 ELLRARRRISSLLRiSSFSLSWDYGAFIRAYAKYLDERLDFHRKLPRDpgtfeRVEYGSLVAVGDPNERYLTMSMEDLLD 155


                  ....*....
gi 2065857412 194 EIDALVGLL 202
Cdd:pfam07651 156 SIPKLQKLL 164
ENTH smart00273
Epsin N-terminal homology (ENTH) domain;
40-166 2.56e-12

Epsin N-terminal homology (ENTH) domain;


Pssm-ID: 214594  Cd Length: 127  Bit Score: 63.42  E-value: 2.56e-12
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412   40 SVLRATTHDPsTPPGNRHLAVILSAGTGSRATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHG--RFILQDQLSVF 117
Cdd:smart00273   6 KVRKATNNDE-WGPKGKHLREIIQGTHNEKSSFAEIMAVLWRRLNDTKNWRVVYKALILLHYLLRNGspRVILEALRNRN 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*....
gi 2065857412  118 pasggrNYLKLSAFRDeKSPLMWELSSWVRWYALYLEHLLSTSRIMGFF 166
Cdd:smart00273  85 ------RILNLSDFQD-IDSRGKDQGANIRTYAKYLLERLEDDRRLKEE 126
ANTH_N_AP180 cd16985
ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of adaptor protein 180 (AP180) ...
 40-153 4.49e-09

ANTH (AP180 N-Terminal Homology) domain, N-terminal region, of adaptor protein 180 (AP180) subfamily; The Adaptor Protein 180 (AP180) subfamily members are phosphatidylinositol-binding clathrin assembly proteins, including mammalian clathrin coat assembly protein AP180 and Clathrin Assembly Lymphoid Myeloid Leukemia protein (CALM), Drosophila LAP (also called Like-AP180 or AP180), and Caenorhabditis elegans Uncoordinated protein 11 (unc-11, also called AP180-like adaptor protein). They are components of the adaptor complexes which link clathrin to receptors in coated vesicles. AP180 and CALM play important roles in clathrin-mediated endocytosis. AP180, also called 91 kDa synaptosomal-associated protein (SNAP91) or phosphoprotein F1-20, is a brain-specific clathrin-binding protein which stimulates clathrin assembly during the recycling of synaptic vesicles. CALM, also called phosphatidylinositol binding clathrin assembly protein (PICALM), is ubiquitously expressed. Members of this subfamily contain ANTH domains, which bind both inositol phospholipids and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. The ANTH domain is a unique module whose N-terminal half is structurally similar to the Epsin N-Terminal Homology (ENTH) and Vps27/Hrs/STAM (VHS) domains, containing a superhelix of eight alpha helices. In addition, it contains a coiled-coil C-terminal half with strutural similarity to spectrin repeats. It binds phosphoinositide PtdIns(4,5)P2 at a short conserved motif K[X]9[K/R][H/Y] between helices 1 and 2. This model describes the N-terminal region of ANTH domains of the Adaptor Protein 180 (AP180) subfamily.


Pssm-ID: 340782  Cd Length: 117  Bit Score: 53.97  E-value: 4.49e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  40 SVLRATTHDPSTPPgNRHLAVILSAGTGSRATASSAVESIMERLHTTgDACVALKSLIIIHHIVKHG--RFI--LQDQLS 115
Cdd:cd16985     4 AVCKATTHEVMGPK-KKHLDYLVQCTNEPNVNIPQLADLLFERTQNS-SWVVVFKALITTHHLMVYGneRFIqyLASRNS 81

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 2065857412 116 VFpasggrnylKLSAFRDEKSPLMWELSSWVRWYALYL 153
Cdd:cd16985    82 LF---------NLSNFLDKSGSQGYDMSTFIRRYAKYL 110
ENTH pfam01417
ENTH domain; The ENTH (Epsin N-terminal homology) domain is found in proteins involved in ...
 41-117 5.81e-04

ENTH domain; The ENTH (Epsin N-terminal homology) domain is found in proteins involved in endocytosis and cytoskeletal machinery. The function of the ENTH domain is unknown.


Pssm-ID: 426255  Cd Length: 124  Bit Score: 39.46  E-value: 5.81e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2065857412  41 VLRATTHDPSTPPGNrHLAVIlsagtgSRATASSA-----VESIMERLHTTGDAC-VALKSLIIIHHIVKHG--RFI--L 110
Cdd:pfam01417   8 VREATNNDPWGPSGT-LMDEI------ARLTYNYVefpeiMKMLWKRLNDKGKNWrHIYKALTLLEYLLKNGseRVVddL 80


                  ....*..
gi 2065857412 111 QDQLSVF 117
Cdd:pfam01417  81 RENIYII 87
ENTH_like_Tepsin cd03572
Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and ...
 41-107 4.37e-03

Epsin N-Terminal Homology (ENTH)-like domain of AP-4 complex accessory subunit Tepsin and similar domains; This family is composed of proteins containing an ENTH-like domain including vertebrate AP-4 complex accessory subunit Tepsin and Arabidopsis thaliana VHS domain-containing protein At3g16270. Tepsin is also called ENTH Domain-containing protein 2 (ENTHD2), Epsin for AP-4, or Tetra-epsin. It associates with the adapter-like complex 4 (AP-4), a heterotetramer composed of two large adaptins (epsilon and beta), a medium adaptin (mu) and a small adaptin (sigma), which forms a non-clathrin coat on vesicles departing the Trans-Golgi Network. The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, and contribute to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.


Pssm-ID: 340773  Cd Length: 119  Bit Score: 36.76  E-value: 4.37e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2065857412  41 VLRATTHDPSTPPGN--RHLAVILSAgtgSRATASSAVESIMERLhTTGDACVALKSLIIIHHIVKHGR 107
Cdd:cd03572     5 LDKATSDDDEPTPGYllEEIAKLTRS---SPGSCQELLDYLLKRL-KKSSPHVKLKALRIIKHLCQKGS 69
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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