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Conserved domains on  [gi|931359025|gb|KPJ51257|]
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MAG: hypothetical protein AMJ41_00110 [candidate division Zixibacteria bacterium DG_27]

Protein Classification

HTH_ARSR and BPL domain-containing protein( domain architecture ID 12089015)

HTH_ARSR and BPL domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 87-326 2.07e-97

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


:

Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 287.84  E-value: 2.07e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  87 RIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVV 166
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 167 DSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDfPKDLRKTATSLAVELGEEVSRL 246
Cdd:COG0340   81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETGKEVDRE 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 247 GVLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKK--GRRRLFLpGGEI 324
Cdd:COG0340  160 ELLAALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETGGETLEGIAVGIDEDGALLLETadGEIRAVA-AGEV 238


                 ..
gi 931359025 325 TH 326
Cdd:COG0340  239 SL 240
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 11-61 8.23e-06

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


:

Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 42.42  E-value: 8.23e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 931359025   11 ILTLLRRSPgEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGY 61
Cdd:pfam08279   3 ILQLLLEAR-GPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 87-326 2.07e-97

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 287.84  E-value: 2.07e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  87 RIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVV 166
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 167 DSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDfPKDLRKTATSLAVELGEEVSRL 246
Cdd:COG0340   81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETGKEVDRE 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 247 GVLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKK--GRRRLFLpGGEI 324
Cdd:COG0340  160 ELLAALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETGGETLEGIAVGIDEDGALLLETadGEIRAVA-AGEV 238


                 ..
gi 931359025 325 TH 326
Cdd:COG0340  239 SL 240
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 87-263 3.28e-68

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 210.97  E-value: 3.28e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  87 RIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVV 166
Cdd:cd16442    1 KLIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPAEAPLLTLLAAVAVA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 167 DSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDFPKDLrktaTSLAVELGEEVSRL 246
Cdd:cd16442   81 EALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVAAVVIGIGINVNNTPPPEPLPD----TSLATSLGKEVDRN 156

                        170
                 ....*....|....*..
gi 931359025 247 GVLKRFLLEFERDYLQF 263
Cdd:cd16442  157 ELLEELLAALENRLELF 173
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 94-326 3.93e-67

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 210.72  E-value: 3.93e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   94 LGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKcGLYFTLILRPPIPPAKAPALSICAALAVVDSLGELf 173
Cdd:TIGR00121   8 IDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILRPDLPKSPAPGLTLVAGIAIAEVLKEL- 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  174 GIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRRedFPKDLRKTATSLAVELGEEVSRLGVLKRFL 253
Cdd:TIGR00121  86 GDQVQVKWPNDILLKDKKLGGILTELTGKENRADYVVIGIGINVQNRK--PAESLREQAISLSEEAGIDLDRGELIEGFL 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 931359025  254 LEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKKGRRRLFLPGGEITH 326
Cdd:TIGR00121 164 RNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIEGIARGIDKDGALLLEDGGGIKKIISGEISL 236
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
10-324 4.76e-65

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 207.72  E-value: 4.76e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  10 EILTLLrrSPGEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGYRYRTAPDLLIASEIREGLKTKlfakRIV 89
Cdd:PRK11886   8 QLLSLL--ADGDFHSGEQLGEELGISRAAIWKHIQTLEEWGLDIFSVKGKGYRLAEPLDLLDPERISSQLPPG----RVT 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  90 SYRTLGSTNEVAKQLAdSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVVDSL 169
Cdd:PRK11886  82 VLPVIDSTNQYLLDRI-AELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRLNQGPAQAMGLSLVVGIAIAEAL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 170 GELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRreDFPKDLRKTATSLAVELGEEVSRLGVL 249
Cdd:PRK11886 161 RRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAAHVVIGIGINVAMP--DFPEELIDQPWSDLQEAGPTIDRNQLA 238

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 931359025 250 KRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKK-GRRRLFLpGGEI 324
Cdd:PRK11886 239 AELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKLIIGDKEISGIARGIDEQGALLLEDdGVEKPFN-GGEI 313
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 96-216 5.42e-21

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 87.11  E-value: 5.42e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   96 STNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCgLYFTLILRPPIPPAKAPALSI---CAALAVVDSLG-- 170
Cdd:pfam03099   7 STNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGC-LTYSLLLSKEHPNVDPSVLEFyvlELVLAVLEALGly 85

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 931359025  171 --ELFGIQLSTKWPNDVLINRKKVCGILTElVTRGNGVDSVIVGVGMN 216
Cdd:pfam03099  86 kpGISGIPCFVKWPNDLYVNGRKLAGILQR-STRGGTLHHGVIGLGVN 132
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 11-61 8.23e-06

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 42.42  E-value: 8.23e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 931359025   11 ILTLLRRSPgEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGY 61
Cdd:pfam08279   3 ILQLLLEAR-GPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
NiaR COG1827
Transcriptional regulator of NAD metabolism, contains HTH and 3H domains [Transcription, ...
 5-81 2.13e-03

Transcriptional regulator of NAD metabolism, contains HTH and 3H domains [Transcription, Coenzyme transport and metabolism];


Pssm-ID: 441432 [Multi-domain]  Cd Length: 171  Bit Score: 38.19  E-value: 2.13e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   5 SEHRDEILTLLRRSPgEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRlGYRYRTAPDL-----LIA-----SE 74
Cdd:COG1827    4 EERREKILEILKESK-EPISGSELAKKFGVSRQVIVQDIALLRAKGEPIIATPR-GYILLKAESSkgftrVIAckhtpEE 81


                 ....*..
gi 931359025  75 IREGLKT 81
Cdd:COG1827   82 IEEELNT 88
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 87-326 2.07e-97

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 287.84  E-value: 2.07e-97
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  87 RIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVV 166
Cdd:COG0340    1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 167 DSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDfPKDLRKTATSLAVELGEEVSRL 246
Cdd:COG0340   81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETGKEVDRE 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 247 GVLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKK--GRRRLFLpGGEI 324
Cdd:COG0340  160 ELLAALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETGGETLEGIAVGIDEDGALLLETadGEIRAVA-AGEV 238


                 ..
gi 931359025 325 TH 326
Cdd:COG0340  239 SL 240
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 87-263 3.28e-68

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 210.97  E-value: 3.28e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  87 RIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVV 166
Cdd:cd16442    1 KLIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPAEAPLLTLLAAVAVA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 167 DSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDFPKDLrktaTSLAVELGEEVSRL 246
Cdd:cd16442   81 EALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVAAVVIGIGINVNNTPPPEPLPD----TSLATSLGKEVDRN 156

                        170
                 ....*....|....*..
gi 931359025 247 GVLKRFLLEFERDYLQF 263
Cdd:cd16442  157 ELLEELLAALENRLELF 173
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 94-326 3.93e-67

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 210.72  E-value: 3.93e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   94 LGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKcGLYFTLILRPPIPPAKAPALSICAALAVVDSLGELf 173
Cdd:TIGR00121   8 IDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILRPDLPKSPAPGLTLVAGIAIAEVLKEL- 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  174 GIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRRedFPKDLRKTATSLAVELGEEVSRLGVLKRFL 253
Cdd:TIGR00121  86 GDQVQVKWPNDILLKDKKLGGILTELTGKENRADYVVIGIGINVQNRK--PAESLREQAISLSEEAGIDLDRGELIEGFL 163

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 931359025  254 LEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKKGRRRLFLPGGEITH 326
Cdd:TIGR00121 164 RNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIEGIARGIDKDGALLLEDGGGIKKIISGEISL 236
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
10-324 4.76e-65

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 207.72  E-value: 4.76e-65
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  10 EILTLLrrSPGEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGYRYRTAPDLLIASEIREGLKTKlfakRIV 89
Cdd:PRK11886   8 QLLSLL--ADGDFHSGEQLGEELGISRAAIWKHIQTLEEWGLDIFSVKGKGYRLAEPLDLLDPERISSQLPPG----RVT 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  90 SYRTLGSTNEVAKQLAdSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVVDSL 169
Cdd:PRK11886  82 VLPVIDSTNQYLLDRI-AELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRLNQGPAQAMGLSLVVGIAIAEAL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 170 GELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRreDFPKDLRKTATSLAVELGEEVSRLGVL 249
Cdd:PRK11886 161 RRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAAHVVIGIGINVAMP--DFPEELIDQPWSDLQEAGPTIDRNQLA 238

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 931359025 250 KRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKK-GRRRLFLpGGEI 324
Cdd:PRK11886 239 AELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKLIIGDKEISGIARGIDEQGALLLEDdGVEKPFN-GGEI 313
BirA COG1654
Biotin operon repressor [Transcription];
5-315 7.86e-62

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 199.83  E-value: 7.86e-62
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   5 SEHRDEILTLLRRspGEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGYRYRTAPDLLIASEIREGLKTKLF 84
Cdd:COG1654    3 SSTRLKLLRLLAD--GEFHSGEELAEELGVSRAAVWKHIKALRELGYEIESVPGKGYRLAEPPDLLDPEEIRAGLSTKRL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  85 AKRIVSYRTLGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALA 164
Cdd:COG1654   81 GREILYVISSTSTNLLALELAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGGGLLYSLLLRPPIAPALLSLLLLAAAVA 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 165 VVDSLGELFGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDFPKDLRKTATSLAVELGEEVS 244
Cdd:COG1654  161 VAAALAEGGGLVKWKKWPNDLLKKGKKILGILEEEGGDADGVVIVVGGGGNNNNSNPEEEPQELAELATSLLLILRLRLL 240

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 931359025 245 RLGVLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKIRCGGKLLSGIAEDIDEEGSLILKKGRR 315
Cdd:COG1654  241 RLLLLLLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVVVEIGGGGGGGGALGGGLLGLLLLGGGGG 311
PRK08330 PRK08330
biotin--protein ligase; Provisional
 86-311 4.29e-58

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 187.26  E-value: 4.29e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  86 KRIVSYRTLGSTNEVAKQLAdSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKcGLYFTLILRPPIPPAKAPALSICAALAV 165
Cdd:PRK08330   3 RNIIYFDEVDSTNEYAKRIA-PDEEEGTVIVADRQTAGHGRKGRAWASPEG-GLWMSVILKPKVSPEHLPKLVFLGALAV 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 166 VDSLGElFGIQLSTKWPNDVLINRKKVCGILTElvtrGNGvDSVIVGVGMNVNNrreDFPKDLRKTATSLAVELGEEVSR 245
Cdd:PRK08330  81 VDTLRE-FGIEGKIKWPNDVLVNYKKIAGVLVE----GKG-DFVVLGIGLNVNN---EIPDELRETATSMKEVLGREVPL 151

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 931359025 246 LGVLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVgLGRSVKIRCGGKLLS-GIAEDIDEEGSLILK 311
Cdd:PRK08330 152 IEVFKRLVENLDRWYKLFLEGPGEILEEVKGRSMI-LGKRVKIIGDGEILVeGIAEDIDEFGALILR 217
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
95-325 3.10e-28

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 111.03  E-value: 3.10e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  95 GSTN----EVAKQLA--DSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCGLYFTLILRPPIPPAKAPALSICAALAVVDS 168
Cdd:PRK06955  42 GSTNadlmARLKALPrsADALPAPIVRVAYEQTAGRGRQGRPWFAQPGNALLFSVACVLPRPVAALAGLSLAVGVALAEA 121

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 169 LGEL---FGIQLSTKWPNDVLINRKKVCGILTELVTRGNGVDSVIVGVGMNVNNRREDFPK--DLRKTATSLAVEL-GEE 242
Cdd:PRK06955 122 LAALpaaLGQRIALKWPNDLLIAGRKLAGILIETVWATPDATAVVIGIGLNVRRADAVAAEvdALRAREAALARGLpPVA 201

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 243 VSRLG-------VLKRFLLEFERDYLQFSKSGLAPFRNHYRECSVGLGRSVKI-RCGGKLLSGIAEDIDEEGSLILKKGR 314
Cdd:PRK06955 202 LAAACaganltdTLAAALNALAPALQAFGADGLAPFAARWHALHAYAGREVVLlEDGAELARGVAHGIDETGQLLLDTPA 281

                        250
                 ....*....|.
gi 931359025 315 RRLFLPGGEIT 325
Cdd:PRK06955 282 GRQAIAAGDVS 292
PRK05935 PRK05935
biotin--protein ligase; Provisional
 96-274 1.80e-21

biotin--protein ligase; Provisional


Pssm-ID: 235649 [Multi-domain]  Cd Length: 190  Bit Score: 89.88  E-value: 1.80e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  96 STNEVAKQLADSGAPEG-TLIVAEEQTKGRGRLGRSWHSPPKcGLYFTLILRPPIPPAKAPALSICAALAVVdSLGELFG 174
Cdd:PRK05935  13 STNTTAKEGMHLWDPYAlTVISTREQTAGKGKFGKSWHSSDQ-DLLASFCFFITVLNIDVSLLFRLGTEAVM-RLGEDLG 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 175 IQLST-KWPNDVLINRKKVCGILTELVTRGNGVdSVIVGVGMNVNNRREDFpKDLRKTATSLAVELGEEVSRLGVLKRFL 253
Cdd:PRK05935  91 ITEAViKWPNDVLVHGEKLCGVLCETIPVKGGL-GVILGIGVNGNTTKDEL-LGIDQPATSLQELLGHPIDLEEQRERLI 168

                        170       180
                 ....*....|....*....|..
gi 931359025 254 LEFERDYLQ-FSKSgLAPFRNH 274
Cdd:PRK05935 169 KHIKHVLIQtLPKL-LARESNH 189
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 96-216 5.42e-21

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 87.11  E-value: 5.42e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   96 STNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSPPKCgLYFTLILRPPIPPAKAPALSI---CAALAVVDSLG-- 170
Cdd:pfam03099   7 STNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKGC-LTYSLLLSKEHPNVDPSVLEFyvlELVLAVLEALGly 85

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 931359025  171 --ELFGIQLSTKWPNDVLINRKKVCGILTElVTRGNGVDSVIVGVGMN 216
Cdd:pfam03099  86 kpGISGIPCFVKWPNDLYVNGRKLAGILQR-STRGGTLHHGVIGLGVN 132
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
113-325 8.79e-19

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 87.07  E-value: 8.79e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 113 TLIVAEEQTKGRGRLGRSW-HSPPKCgLYFTLILRPPIPPAKAPALSICAALAVVDSLGELfGIQLSTKWPNDVLINRKK 191
Cdd:PRK13325 111 TICVTHLQSKGRGRQGRKWsHRLGEC-LMFSFGWVFDRPQYELGSLSPVAAVACRRALSRL-GLKTQIKWPNDLVVGRDK 188

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 192 VCGILTELVtRGNGVDSVIVGVGMNVNnrredFPKDLRKTATslAVELGEEVSRLG------VLKRFLLEFERDYLQFSK 265
Cdd:PRK13325 189 LGGILIETV-RTGGKTVAVVGIGINFV-----LPKEVENAAS--VQSLFQTASRRGnadaavLLETLLAELDAVLLQYAR 260

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 931359025 266 SGLAPFRNHYRECSVGLGRSV-KIRCGGKLLSGIAEDIDEEGSLILKKGRRRLFLPGGEIT 325
Cdd:PRK13325 261 DGFAPFVAEYQAANRDHGKAVlLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEIS 321
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 94-217 3.03e-17

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 79.91  E-value: 3.03e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  94 LGSTNEVAKQLADSGAPEGTLIVAEEQTKGRGRLGRSWHSpPKCGLYFTL-ILRPPIPPAKAPALSICAALAVVDSLGEL 172
Cdd:PTZ00276  15 VTSTMDVARTMLAAAGGKPFAVLAESQTAGRGTGGRTWTS-PKGNMYFTLcIPQKGVPPELVPVLPLITGLACRAAIMEV 93

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
gi 931359025 173 F-GIQLSTKWPNDVLINRKKVCGILTElvtrgNGVDSVIVGVGMNV 217
Cdd:PTZ00276  94 LhGAAVHTKWPNDIIYAGKKIGGSLIE-----SEGEYLIIGIGMNI 134
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
88-311 7.35e-11

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 60.74  E-value: 7.35e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025  88 IVSYRTLGSTNEVAKQLADSG---APegTLIVAEEQTKGRGRLGRSWHSPpKCGLYFTLILRPPIPPA--KAPALSICAA 162
Cdd:PRK08477   3 IRVFESLDSTQTYLIEKIKNGelkAP--FAIVAKEQTAGIGSRGNSWEGK-KGNLFFSFALKESDLPKdlPLQSSSIYFG 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 163 LAVVDSLGELfGIQLSTKWPNDVLINRKKVCGILTELVTrgngvDSVIVGVGMNVNNRREDFPKdlrktatslaveLGEE 242
Cdd:PRK08477  80 FLLKEVLKEL-GSKVWLKWPNDLYLDDKKIGGVITNKIK-----NFIVCGIGLNLKFSPKNFAC------------LDIE 141

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 931359025 243 VSRLGVLKRFLLEFE---------RDY-LQFSKSglapfrnhyrecsvglgRSVKIRCGGKLLSGIAEDIDEEGSLILK 311
Cdd:PRK08477 142 ISDDLLLEGFLQKIEkkilwkqifSKYkLEFEKS-----------------KSFSFHIDGKLVSLKDAELLEDGSILIN 203
BPL_LplA_LipB cd16435
biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), ...
 124-256 8.58e-08

biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.


Pssm-ID: 319740  Cd Length: 198  Bit Score: 51.77  E-value: 8.58e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 124 RGRLGRSWHSPPKcGLYFTLILRPPIPPAKAPALSIcAALAVVDSLGElFGIQLSTKW-PNDVLINRKKVCGILTELVTr 202
Cdd:cd16435   68 RNRGGRAVSHDPG-QLVFSPVIGPNVEFMISKFNLI-IEEGIRDAIAD-FGQSAEVKWgRNDLWIDNRKVCGIAVRVVK- 143

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 931359025 203 gngvDSVIVGVGMNVNNRREDFPKDLR-----KTATSLAVELGEEVSRLGVLKRFLLEF 256
Cdd:cd16435  144 ----EAIFHGIALNLNQDLENFTEIIPcgykpERVTSLSLELGRKVTVEQVLERVLAAF 198
PTZ00275 PTZ00275
biotin-acetyl-CoA-carboxylase ligase; Provisional
 111-317 5.24e-07

biotin-acetyl-CoA-carboxylase ligase; Provisional


Pssm-ID: 185536 [Multi-domain]  Cd Length: 285  Bit Score: 50.21  E-value: 5.24e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 111 EGTLIVA-EEQTKGRG------RLGRSWHSPpKCGLYFTLI-LRPPIPPAKAPALSICAALAVVDSLgELFGIQLSTKWP 182
Cdd:PTZ00275  48 DNMIIVScNEQTNGIGtrdtkkNQDRIWLSE-KGNLFTTFVfLWNRNDIEKVKYLAQTCTVAISKTL-EYFHLVTQIKWI 125

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025 183 NDVLINRKKVCGILTELVTRGNGVD------SVIVGVGMNVNNRREDfpKDLRKTATSLAVELGEEV-------SRLGVL 249
Cdd:PTZ00275 126 NDVLVNYKKIAGCLVHLYYLDDFPNlnsryvCVMVGIGINVTLEDKH--NLLNNNYTSIKKELQRDFntpksipSVEQVT 203

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 931359025 250 KRFLLEFERDYLQFSKSGLAPFRNH------YREcsvglgRSVKIRCGGKLLSGIAEDIDEEGSLILKKGRRRL 317
Cdd:PTZ00275 204 EKLIINLKAVINKLRKEGFSSFLDYitprllYKD------KKVLIDQDNELIVGYLQGLLHDGSLLLLREKNKL 271
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 11-61 8.23e-06

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 42.42  E-value: 8.23e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 931359025   11 ILTLLRRSPgEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRLGY 61
Cdd:pfam08279   3 ILQLLLEAR-GPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
BPL_C pfam02237
Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It ...
 282-326 1.89e-03

Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It is found to the C terminus of the biotin protein ligase catalytic domain pfam01317.


Pssm-ID: 426672 [Multi-domain]  Cd Length: 48  Bit Score: 35.90  E-value: 1.89e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*..
gi 931359025  282 LGRSVKIRCGGKLLSGIAEDIDEEGSLILK--KGRRRLFlPGGEITH 326
Cdd:pfam02237   2 LGREVRVLLGDGIVEGIAVGIDDDGALLLEtdDGTIRDI-NSGEVSL 47
NiaR COG1827
Transcriptional regulator of NAD metabolism, contains HTH and 3H domains [Transcription, ...
 5-81 2.13e-03

Transcriptional regulator of NAD metabolism, contains HTH and 3H domains [Transcription, Coenzyme transport and metabolism];


Pssm-ID: 441432 [Multi-domain]  Cd Length: 171  Bit Score: 38.19  E-value: 2.13e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 931359025   5 SEHRDEILTLLRRSPgEYVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDRRlGYRYRTAPDL-----LIA-----SE 74
Cdd:COG1827    4 EERREKILEILKESK-EPISGSELAKKFGVSRQVIVQDIALLRAKGEPIIATPR-GYILLKAESSkgftrVIAckhtpEE 81


                 ....*..
gi 931359025  75 IREGLKT 81
Cdd:COG1827   82 IEEELNT 88
YobV COG2378
Predicted DNA-binding transcriptional regulator YobV, contains HTH and WYL domains ...
 10-69 2.39e-03

Predicted DNA-binding transcriptional regulator YobV, contains HTH and WYL domains [Transcription];


Pssm-ID: 441945 [Multi-domain]  Cd Length: 314  Bit Score: 39.29  E-value: 2.39e-03
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 931359025  10 EILTLLRRSPGeyVSGRFLAESLDLKRESIYHTISQLRQFGYGIESDR--RLGYRYRTAPDL 69
Cdd:COG2378    9 ALLQLLQSRRG--VTAAELAERLEVSERTIYRDIDALRELGVPIEAERgrGGGYRLRDGYRL 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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