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Conserved domains on  [gi|66773062|ref|NP_001019611|]
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kinetochore protein Spc24 [Danio rerio]

Protein Classification

kinetochore Spc24 family protein( domain architecture ID 10550629)

kinetochore Spc24 family protein acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity

Gene Ontology:  GO:0000776|GO:0051301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 87-197 2.81e-19

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


:

Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 78.80  E-value: 2.81e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062    87 KMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVDEDTTEVIPSAIYVAQLYSKVtKIKLEFDTEPHILK 166
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 66773062   167 GVHYGadVVTPINIDTStRTPCEVSDELWSL 197
Cdd:pfam08286  80 NKDKG--DVHVVNLDPK-LSDFFYANYLWDL 107
PRK12704 super family cl36166
phosphodiesterase; Provisional
 27-137 3.82e-04

phosphodiesterase; Provisional


The actual alignment was detected with superfamily member PRK12704:

Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 40.53  E-value: 3.82e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062   27 KAESVLQAARKKKQEffdhyIKTKKTLTelLNDLVHtegvvgQKLLDLDQQKSQMMQEIMKMEQEVQRsqsKSQNLDSEL 106
Cdd:PRK12704  39 EAKRILEEAKKEAEA-----IKKEALLE--AKEEIH------KLRNEFEKELRERRNELQKLEKRLLQ---KEENLDRKL 102

                         90       100       110
                 ....*....|....*....|....*....|.
gi 66773062  107 EFLQKELERLRDAEQEIQTLQQEVDEDTTEV 137
Cdd:PRK12704 103 ELLEKREEELEKKEKELEQKQQELEKKEEEL 133
 
Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 87-197 2.81e-19

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 78.80  E-value: 2.81e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062    87 KMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVDEDTTEVIPSAIYVAQLYSKVtKIKLEFDTEPHILK 166
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 66773062   167 GVHYGadVVTPINIDTStRTPCEVSDELWSL 197
Cdd:pfam08286  80 NKDKG--DVHVVNLDPK-LSDFFYANYLWDL 107
RWD_Spc24 cd11565
RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; ...
 141-198 3.28e-05

RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; The kinetochore protein Spc24 acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Spc24 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. The model corresponds to the Spc24 RWD domain, which provides an interaction platform to mediate kinetochore assembly.


Pssm-ID: 467842  Cd Length: 58  Bit Score: 40.19  E-value: 3.28e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062 141 AIYVAQLYSKVtKIKLEFDTEPHILKGVHYG--ADVVTPINIDtSTRTPCEVSDELWSLV 198
Cdd:cd11565   1 TVLKLKLYRSL-GIELEEDEDGEYDKALIRNknKGDVHVVNLD-DKYSDYFYSNYLWDLL 58
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 69-137 3.80e-04

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.58  E-value: 3.80e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 66773062  69 QKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELErlrDAEQEIQTLQQEVDEDTTEV 137
Cdd:COG3883  23 KELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEID---KLQAEIAEAEAEIEERREEL 88
PRK12704 PRK12704
phosphodiesterase; Provisional
 27-137 3.82e-04

phosphodiesterase; Provisional


Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 40.53  E-value: 3.82e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062   27 KAESVLQAARKKKQEffdhyIKTKKTLTelLNDLVHtegvvgQKLLDLDQQKSQMMQEIMKMEQEVQRsqsKSQNLDSEL 106
Cdd:PRK12704  39 EAKRILEEAKKEAEA-----IKKEALLE--AKEEIH------KLRNEFEKELRERRNELQKLEKRLLQ---KEENLDRKL 102

                         90       100       110
                 ....*....|....*....|....*....|.
gi 66773062  107 EFLQKELERLRDAEQEIQTLQQEVDEDTTEV 137
Cdd:PRK12704 103 ELLEKREEELEKKEKELEQKQQELEKKEEEL 133
SMC_prok_B TIGR02168
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 9-130 3.05e-03

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 38.11  E-value: 3.05e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062      9 DDDLEKLGESLNEIimsSKAESVLQAARKKKQEFfdhyiktkKTLTELLNDLVHTegVVGQKLLDLDQQKSQMMQEIMKM 88
Cdd:TIGR02168  185 RENLDRLEDILNEL---ERQLKSLERQAEKAERY--------KELKAELRELELA--LLVLRLEELREELEELQEELKEA 251

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|..
gi 66773062     89 EQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEV 130
Cdd:TIGR02168  252 EEELEELTAELQELEEKLEELRLEVSELEEEIEELQKELYAL 293
 
Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 87-197 2.81e-19

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 78.80  E-value: 2.81e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062    87 KMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVDEDTTEVIPSAIYVAQLYSKVtKIKLEFDTEPHILK 166
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 66773062   167 GVHYGadVVTPINIDTStRTPCEVSDELWSL 197
Cdd:pfam08286  80 NKDKG--DVHVVNLDPK-LSDFFYANYLWDL 107
RWD_Spc24 cd11565
RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; ...
 141-198 3.28e-05

RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; The kinetochore protein Spc24 acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Spc24 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. The model corresponds to the Spc24 RWD domain, which provides an interaction platform to mediate kinetochore assembly.


Pssm-ID: 467842  Cd Length: 58  Bit Score: 40.19  E-value: 3.28e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062 141 AIYVAQLYSKVtKIKLEFDTEPHILKGVHYG--ADVVTPINIDtSTRTPCEVSDELWSLV 198
Cdd:cd11565   1 TVLKLKLYRSL-GIELEEDEDGEYDKALIRNknKGDVHVVNLD-DKYSDYFYSNYLWDLL 58
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 69-137 3.80e-04

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.58  E-value: 3.80e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 66773062  69 QKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELErlrDAEQEIQTLQQEVDEDTTEV 137
Cdd:COG3883  23 KELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEID---KLQAEIAEAEAEIEERREEL 88
PRK12704 PRK12704
phosphodiesterase; Provisional
 27-137 3.82e-04

phosphodiesterase; Provisional


Pssm-ID: 237177 [Multi-domain]  Cd Length: 520  Bit Score: 40.53  E-value: 3.82e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062   27 KAESVLQAARKKKQEffdhyIKTKKTLTelLNDLVHtegvvgQKLLDLDQQKSQMMQEIMKMEQEVQRsqsKSQNLDSEL 106
Cdd:PRK12704  39 EAKRILEEAKKEAEA-----IKKEALLE--AKEEIH------KLRNEFEKELRERRNELQKLEKRLLQ---KEENLDRKL 102

                         90       100       110
                 ....*....|....*....|....*....|.
gi 66773062  107 EFLQKELERLRDAEQEIQTLQQEVDEDTTEV 137
Cdd:PRK12704 103 ELLEKREEELEKKEKELEQKQQELEKKEEEL 133
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 75-132 6.20e-04

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 39.81  E-value: 6.20e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 66773062  75 DQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELERLR----DAEQEIQTLQQEVDE 132
Cdd:COG3883  15 DPQIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQaeleALQAEIDKLQAEIAE 76
GumC COG3206
Exopolysaccharide export protein/domain GumC/Wzc1 [Cell wall/membrane/envelope biogenesis];
69-131 1.81e-03

Exopolysaccharide export protein/domain GumC/Wzc1 [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442439 [Multi-domain]  Cd Length: 687  Bit Score: 38.46  E-value: 1.81e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 66773062  69 QKLLDLDQQKSQMMQEIM-KMEQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEVD 131
Cdd:COG3206 298 AQIAALRAQLQQEAQRILaSLEAELEALQAREASLQAQLAQLEARLAELPELEAELRRLEREVE 361
EnvC COG4942
Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, ...
 48-156 1.99e-03

Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 443969 [Multi-domain]  Cd Length: 377  Bit Score: 38.21  E-value: 1.99e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062  48 KTKKTLTELLNDLVHTEgvvgQKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELERLrdaEQEIQTLQ 127
Cdd:COG4942  24 EAEAELEQLQQEIAELE----KELAALKKEEKALLKQLAALERRIAALARRIRALEQELAALEAELAEL---EKEIAELR 96

                        90       100
                ....*....|....*....|....*....
gi 66773062 128 QEVDEDTTEVIpSAIYVAQLYSKVTKIKL 156
Cdd:COG4942  97 AELEAQKEELA-ELLRALYRLGRQPPLAL 124
HMMR_N pfam15905
Hyaluronan mediated motility receptor N-terminal; HMMR_N is the N-terminal region of ...
 38-136 2.86e-03

Hyaluronan mediated motility receptor N-terminal; HMMR_N is the N-terminal region of eukaryotic hyaluronan-mediated motility receptor proteins. The protein is functionally associated with BRCA1 and thus predicted to be a common, low-penetrance breast cancer candidate.


Pssm-ID: 464932 [Multi-domain]  Cd Length: 329  Bit Score: 37.87  E-value: 2.86e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062    38 KKQEFFDHYIKTKKTLTELLNDLVHTEGVVGQ---KLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKELE 114
Cdd:pfam15905 171 KMKEVMAKQEGMEGKLQVTQKNLEHSKGKVAQleeKLVSTEKEKIEEKSETEKLLEYITELSCVSEQVEKYKLDIAQLEE 250

                          90       100
                  ....*....|....*....|..
gi 66773062   115 RLRDAEQEIQTLQQEVDEDTTE 136
Cdd:pfam15905 251 LLKEKNDEIESLKQSLEEKEQE 272
EnvC COG4942
Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, ...
 69-136 2.88e-03

Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 443969 [Multi-domain]  Cd Length: 377  Bit Score: 37.82  E-value: 2.88e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 66773062  69 QKLLDLDQQKSQMMQEIMKMEQEVQRSQSKSQNLDSELEFLQKEL----ERLRDAEQEIQTLQQEVDEDTTE 136
Cdd:COG4942  20 DAAAEAEAELEQLQQEIAELEKELAALKKEEKALLKQLAALERRIaalaRRIRALEQELAALEAELAELEKE 91
SMC_prok_B TIGR02168
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 9-130 3.05e-03

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 38.11  E-value: 3.05e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 66773062      9 DDDLEKLGESLNEIimsSKAESVLQAARKKKQEFfdhyiktkKTLTELLNDLVHTegVVGQKLLDLDQQKSQMMQEIMKM 88
Cdd:TIGR02168  185 RENLDRLEDILNEL---ERQLKSLERQAEKAERY--------KELKAELRELELA--LLVLRLEELREELEELQEELKEA 251

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|..
gi 66773062     89 EQEVQRSQSKSQNLDSELEFLQKELERLRDAEQEIQTLQQEV 130
Cdd:TIGR02168  252 EEELEELTAELQELEEKLEELRLEVSELEEEIEELQKELYAL 293
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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