RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1883684682   1 MQLSQDYNNEDEFGRKLRFLLQQLPPVNYSLLKFLCRFLANVASHH-EEIWSANSLAAVFGPDVFHIYTDVEDMKEQEIV 79
Cdd:cd04393   100 MQLYQDYNGEDEFGRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHhENRMTAENLAAVFGPDVFHVYTDVEDMKEQEIC 179

                  ....*...
gi 1883684682  80 SRIMAGLL 87
Cdd:cd04393   180 SRIMAKLL 187

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1883684682   1 MQLSQDYNNEDEFGRKLRFLLQQLPPVNYSLLKFLCRFLANVASHH-EEIWSANSLAAVFGPDVFHIYTDVEDMKEQEIV 79
Cdd:cd04393   100 MQLYQDYNGEDEFGRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHhENRMTAENLAAVFGPDVFHVYTDVEDMKEQEIC 179

                  ....*...
gi 1883684682  80 SRIMAGLL 87
Cdd:cd04393   180 SRIMAKLL 187

GTPase-activator protein for Rho-like GTPases; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. etter domain limits and outliers.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1883684682    8 NNEDEFGRKLRFLLQQLPPVNYSLLKFLCRFLANVASHHEE-IWSANSLAAVFGPDVFH-IYTDVEDMKEQEIVSRIM 83
Cdd:smart00324  90 EDETERLRALRELLSLLPPANRATLRYLLAHLNRVAEHSEEnKMTARNLAIVFGPTLLRpPDGEVASLKDIRHQNTVI 167

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1883684682   1 MQLSQDYNNEDEFGRKLRFLLQQLPPVNYSLLKFLCRFLANVASHH-EEIWSANSLAAVFGPDVFHIYTDVEDMKEQEIV 79
Cdd:cd04393   100 MQLYQDYNGEDEFGRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHhENRMTAENLAAVFGPDVFHVYTDVEDMKEQEIC 179

                  ....*...
gi 1883684682  80 SRIMAGLL 87
Cdd:cd04393   180 SRIMAKLL 187

GTPase-activator protein for Rho-like GTPases; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. etter domain limits and outliers.

                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1883684682    8 NNEDEFGRKLRFLLQQLPPVNYSLLKFLCRFLANVASHHEE-IWSANSLAAVFGPDVFH-IYTDVEDMKEQEIVSRIM 83
Cdd:smart00324  90 EDETERLRALRELLSLLPPANRATLRYLLAHLNRVAEHSEEnKMTARNLAIVFGPTLLRpPDGEVASLKDIRHQNTVI 167

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1883684682  16 KLRFLLQQLPPVNYSLLKFLCRFLANVASHHEE-IWSANSLAAVFGPDVFHiyTDVED----MKEQEIVSRIM 83
Cdd:cd04390   118 ELMKQVSILPKVNYNLLSYICRFLDEVQSNSSVnKMSVQNLATVFGPNILR--PKVEDpatiMEGTPQIQQLM 188

RhoGAP_ARHGAP27_15_12_9: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP27 (also called CAMGAP1), ARHGAP15, 12 and 9-like proteins; This subgroup of ARHGAPs are multidomain proteins that contain RhoGAP, PH, SH3 and WW domains. Most members that are studied show GAP activity towards Rac1, some additionally show activity towards Cdc42. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1883684682  15 RKLRFLLQQLPPVNYSLLKFLCRFLANVASHHEE-IWSANSLAAVFGPDVFH 65
Cdd:cd04403   111 SAVKDLIKSLPKPNHDTLKMLFRHLCRVIEHGEKnRMTTQNLAIVFGPTLLR 162

RhoGAP_ARHGAP21: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP21-like proteins. ArhGAP21 is a multi-domain protein, containing RhoGAP, PH and PDZ domains, and is believed to play a role in the organization of the cell-cell junction complex. It has been shown to function as a GAP of Cdc42 and RhoA, and to interact with alpha-catenin and Arf6. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1883684682  15 RKLRFLLQQLPPVNYSLLKFLCRFLANVASHHE-EIWSANSLAAVFGPDVFHIYTD-----VEDMKEQ-EIV 79
Cdd:cd04395   114 KELRRLIHSLPDHHYETLKHLIRHLKTVADNSEvNKMEPRNLAIVFGPTLVRTSDDnmetmVTHMPDQcKIV 185

N-terminal homeodomain-like domain of metazoan RecQ protein-like 4 (RecQL4), fungal DNA replication regulator SLD2 and similar proteins; RecQL4, also called ATP-dependent DNA helicase Q4, or DNA helicase, RecQ-like type 4 (RecQ4), or RTS, is a DNA-dependent ATPase that may modulate chromosome segregation. This family also includes fungal DNA replication regulator SLD2, also known as DNA replication and checkpoint protein 1 (DRC1), which functions with DPB11 to control DNA replication and the S-phase checkpoint. It is also required for the proper activation of RAD53 in response to DNA damage and replication blocks. This model corresponds to the N-terminal domain of RecQL4 and SLD2, which is a homeodomain-like DNA interaction motif.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....
gi 1883684682 679 KLRKTLREFEEAFYQQNGRNAQKEDRVPVLEE----YREYKKIK 718
Cdd:cd22289     5 ELKIALKTWERAFAKKHGRKPTKDDIKAAPEEikdlYKEYAKLK 48

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1883684682   5 QDYNNEDEFGRK--LRFLLQQLPPVNYSLLKFLCRFLANVASHHEEI-WSANSLAAVFGPD 62
Cdd:cd04386   103 QAANKPDEDERLqaIWRILNKLPRENRDNLRYLIKFLSKLAQKSDENkMSPSNIAIVLAPN 163

RhoGAP_Graf: GTPase-activator protein (GAP) domain for Rho-like GTPases found in GRAF (GTPase regulator associated with focal adhesion kinase); Graf is a multi-domain protein, containing SH3 and PH domains, that binds focal adhesion kinase and influences cytoskeletal changes mediated by Rho proteins. Graf exhibits GAP activity toward RhoA and Cdc42, but only weakly activates Rac1. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1883684682  19 FLLQQLPPVNYSLLKFLCRFLANVASHHEE-IWSANSLAAVFGPDVFHIYTD----VEDMKEQEIVSRIM 83
Cdd:cd04374   131 SLVHKLPEKNREMLELLIKHLTNVSDHSKKnLMTVSNLGVVFGPTLLRPQEEtvaaIMDIKFQNIVVEIL 200