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Conserved domains on  [gi|2236172485|ref|NP_001392952|]
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2'-5'-oligoadenylate synthase 1 isoform 8 [Homo sapiens]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OAS1_C super family cl11075
2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, ...
 210-335 4.72e-94

2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, C-terminal half of 2'-5'-oligoadenylate synthetase 1, being described as domain 2 of the enzyme and homologous to a tandem ubiquitin repeat. It carries the region of enzymic activity between 320 and 344 at the extreme C-terminal end. Oligoadenylate synthetases are antiviral enzymes that counteract vial attack by degrading viral RNA. The enzyme uses ATP in 2'-specific nucleotidyl transfer reactions to synthesize 2'.5'-oligoadenylates, which activate latent ribonuclease, resulting in degradation of viral RNA and inhibition of virus replication. This domain is often associated with NTP_transf_2 pfam01909.


The actual alignment was detected with superfamily member pfam10421:

Pssm-ID: 463087  Cd Length: 188  Bit Score: 278.99  E-value: 4.72e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485 210 LCKKKLGK--LPPQYALELLTVYAWERGSMKTHFNTAQGFRTVLELVINYQQLCIYWTKYYDFKNPIIEKYLRRQLTKPR 287
Cdd:pfam10421  56 QCKKKLKGasLPPQYALELLTVYAWEQGCGKEDFNTAEGFRTVLELIQQYQQLCIYWTVYYDFEDETVRNFLLKQLKKPR 135

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 288 PVILDPADPTGNLGGGDPKGWRQLAQEAEAWLNYPCFKNWDGSPVSSW 335
Cdd:pfam10421 136 PVILDPADPTGNVGGGDRWRWDLLAQEAAAWLSQPCFKNGDGSPVPSW 183
NT_2-5OAS_ClassI-CCAase cd05400
Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class ...
 29-156 2.48e-21

Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class I CCA-adding enzyme; In vertebrates, 2-5OASs are induced by interferon during the innate immune response to protect against RNA virus infections. In the presence of an RNA activator, 2-5OASs catalyze the oligomerization of ATP into 2-5A. 2-5A activates endoribonuclease L, which leads to degradation of the viral RNA. 2-5OASs are also implicated in cell growth control, differentiation, and apoptosis. This family includes human OAS1, -2, -3, and OASL. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This class I group includes the archaeal Sulfolobus shibatae and Archeoglobus fulgidus CCA-adding enzymes. It belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this family.


:

Pssm-ID: 143390 [Multi-domain]  Cd Length: 143  Bit Score: 89.00  E-value: 2.48e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485  29 QINHAIDIICGFLKERCFRGSSYpvcVSKVVKGGSSGKGTTLRGRSDADLVVFLSPLTTFQDqlNRRGEFIQEIRRQLEA 108
Cdd:cd05400     4 EAKERYREIREALKESLSELAGR---VAEVFLQGSYARGTALRGDSDIDLVVVLPDDTSFAE--YGPAELLDELGEALKE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 109 CQRERAfsvkfEVQAPRwgnpRALSFVLSslqlGEGVEFDVLPAFDAL 156
Cdd:cd05400    79 YYGANE-----EVKAQH----RSVTVKFK----GQGFHVDVVPAFEAD 113
 
Name Accession Description Interval E-value
OAS1_C pfam10421
2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, ...
 210-335 4.72e-94

2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, C-terminal half of 2'-5'-oligoadenylate synthetase 1, being described as domain 2 of the enzyme and homologous to a tandem ubiquitin repeat. It carries the region of enzymic activity between 320 and 344 at the extreme C-terminal end. Oligoadenylate synthetases are antiviral enzymes that counteract vial attack by degrading viral RNA. The enzyme uses ATP in 2'-specific nucleotidyl transfer reactions to synthesize 2'.5'-oligoadenylates, which activate latent ribonuclease, resulting in degradation of viral RNA and inhibition of virus replication. This domain is often associated with NTP_transf_2 pfam01909.


Pssm-ID: 463087  Cd Length: 188  Bit Score: 278.99  E-value: 4.72e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485 210 LCKKKLGK--LPPQYALELLTVYAWERGSMKTHFNTAQGFRTVLELVINYQQLCIYWTKYYDFKNPIIEKYLRRQLTKPR 287
Cdd:pfam10421  56 QCKKKLKGasLPPQYALELLTVYAWEQGCGKEDFNTAEGFRTVLELIQQYQQLCIYWTVYYDFEDETVRNFLLKQLKKPR 135

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 288 PVILDPADPTGNLGGGDPKGWRQLAQEAEAWLNYPCFKNWDGSPVSSW 335
Cdd:pfam10421 136 PVILDPADPTGNVGGGDRWRWDLLAQEAAAWLSQPCFKNGDGSPVPSW 183
NT_2-5OAS_ClassI-CCAase cd05400
Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class ...
 29-156 2.48e-21

Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class I CCA-adding enzyme; In vertebrates, 2-5OASs are induced by interferon during the innate immune response to protect against RNA virus infections. In the presence of an RNA activator, 2-5OASs catalyze the oligomerization of ATP into 2-5A. 2-5A activates endoribonuclease L, which leads to degradation of the viral RNA. 2-5OASs are also implicated in cell growth control, differentiation, and apoptosis. This family includes human OAS1, -2, -3, and OASL. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This class I group includes the archaeal Sulfolobus shibatae and Archeoglobus fulgidus CCA-adding enzymes. It belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this family.


Pssm-ID: 143390 [Multi-domain]  Cd Length: 143  Bit Score: 89.00  E-value: 2.48e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485  29 QINHAIDIICGFLKERCFRGSSYpvcVSKVVKGGSSGKGTTLRGRSDADLVVFLSPLTTFQDqlNRRGEFIQEIRRQLEA 108
Cdd:cd05400     4 EAKERYREIREALKESLSELAGR---VAEVFLQGSYARGTALRGDSDIDLVVVLPDDTSFAE--YGPAELLDELGEALKE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 109 CQRERAfsvkfEVQAPRwgnpRALSFVLSslqlGEGVEFDVLPAFDAL 156
Cdd:cd05400    79 YYGANE-----EVKAQH----RSVTVKFK----GQGFHVDVVPAFEAD 113
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 37-125 5.35e-06

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 44.33  E-value: 5.35e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485  37 ICGFLKERCFRGSsypvcVSKVVKGGSSGKGTTLRGrSDADLVVFlSPLTTFQDQLNRRGEFIQEIRRQLeacQRERAFS 116
Cdd:pfam01909   1 LRKLREILKELFP-----VAEVVLFGSYARGTALPG-SDIDLLVV-FPEPVEEERLLKLAKIIKELEELL---GLEVDLV 70


                  ....*....
gi 2236172485 117 VKFEVQAPR 125
Cdd:pfam01909  71 TREKIEFPL 79
 
Name Accession Description Interval E-value
OAS1_C pfam10421
2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, ...
 210-335 4.72e-94

2'-5'-oligoadenylate synthetase 1, domain 2, C-terminus; This is the largely alpha-helical, C-terminal half of 2'-5'-oligoadenylate synthetase 1, being described as domain 2 of the enzyme and homologous to a tandem ubiquitin repeat. It carries the region of enzymic activity between 320 and 344 at the extreme C-terminal end. Oligoadenylate synthetases are antiviral enzymes that counteract vial attack by degrading viral RNA. The enzyme uses ATP in 2'-specific nucleotidyl transfer reactions to synthesize 2'.5'-oligoadenylates, which activate latent ribonuclease, resulting in degradation of viral RNA and inhibition of virus replication. This domain is often associated with NTP_transf_2 pfam01909.


Pssm-ID: 463087  Cd Length: 188  Bit Score: 278.99  E-value: 4.72e-94
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485 210 LCKKKLGK--LPPQYALELLTVYAWERGSMKTHFNTAQGFRTVLELVINYQQLCIYWTKYYDFKNPIIEKYLRRQLTKPR 287
Cdd:pfam10421  56 QCKKKLKGasLPPQYALELLTVYAWEQGCGKEDFNTAEGFRTVLELIQQYQQLCIYWTVYYDFEDETVRNFLLKQLKKPR 135

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 288 PVILDPADPTGNLGGGDPKGWRQLAQEAEAWLNYPCFKNWDGSPVSSW 335
Cdd:pfam10421 136 PVILDPADPTGNVGGGDRWRWDLLAQEAAAWLSQPCFKNGDGSPVPSW 183
NT_2-5OAS_ClassI-CCAase cd05400
Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class ...
 29-156 2.48e-21

Nucleotidyltransferase (NT) domain of 2'5'-oligoadenylate (2-5A)synthetase (2-5OAS) and class I CCA-adding enzyme; In vertebrates, 2-5OASs are induced by interferon during the innate immune response to protect against RNA virus infections. In the presence of an RNA activator, 2-5OASs catalyze the oligomerization of ATP into 2-5A. 2-5A activates endoribonuclease L, which leads to degradation of the viral RNA. 2-5OASs are also implicated in cell growth control, differentiation, and apoptosis. This family includes human OAS1, -2, -3, and OASL. CCA-adding enzymes add the sequence [cytidine(C)-cytidine-adenosine (A)], one nucleotide at a time, onto the 3' end of tRNA, in a template-independent reaction. This class I group includes the archaeal Sulfolobus shibatae and Archeoglobus fulgidus CCA-adding enzymes. It belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, coordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this family.


Pssm-ID: 143390 [Multi-domain]  Cd Length: 143  Bit Score: 89.00  E-value: 2.48e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485  29 QINHAIDIICGFLKERCFRGSSYpvcVSKVVKGGSSGKGTTLRGRSDADLVVFLSPLTTFQDqlNRRGEFIQEIRRQLEA 108
Cdd:cd05400     4 EAKERYREIREALKESLSELAGR---VAEVFLQGSYARGTALRGDSDIDLVVVLPDDTSFAE--YGPAELLDELGEALKE 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 2236172485 109 CQRERAfsvkfEVQAPRwgnpRALSFVLSslqlGEGVEFDVLPAFDAL 156
Cdd:cd05400    79 YYGANE-----EVKAQH----RSVTVKFK----GQGFHVDVVPAFEAD 113
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 37-125 5.35e-06

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 44.33  E-value: 5.35e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2236172485  37 ICGFLKERCFRGSsypvcVSKVVKGGSSGKGTTLRGrSDADLVVFlSPLTTFQDQLNRRGEFIQEIRRQLeacQRERAFS 116
Cdd:pfam01909   1 LRKLREILKELFP-----VAEVVLFGSYARGTALPG-SDIDLLVV-FPEPVEEERLLKLAKIIKELEELL---GLEVDLV 70


                  ....*....
gi 2236172485 117 VKFEVQAPR 125
Cdd:pfam01909  71 TREKIEFPL 79
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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