Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  33 KSRWRSIRILYLTMFLSSVGFSVVMmSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSIL 112
Cdd:COG2814     6 RRRWLALLALALGAFLSGLGIGIVL-PALPLIAA-DLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLGLL 83

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 2286439362 113 ISVAANCLYAylhipASHNKYYMLVARGLLGIGA--VFQTCFTFLGE 157
Cdd:COG2814    84 LFALGSLLCA-----LAPSLWLLLAARFLQGLGAgaLFPAALALIAD 125

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  42 LYLTMFLSSVGFSVVMMSIWPYLQKI-DPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCL 120
Cdd:cd17330     1 LFLVVFLDALGFGIVFPVLPFYVREFnKLGASGYYVGLLQSSYSLGQFLASPLWGRLSDRYGRKPVLLLSLAGSAVSYVL 80

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 2286439362 121 YAYlhipaSHNKYYMLVARGLLGI-GAVFQTCFTFLGE 157
Cdd:cd17330    81 LGL-----SRNLWLLFLARFLDGLtGGNVSVAQAYIAD 113

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  42 LYLTMFLSSVGFSVVMMSIWPYLQKIDptADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLY 121
Cdd:cd17325     1 LSLALFLDMLGYGIIIPILPLYAESLG--ASVAQIGLIVAAYALAQLLFSPPAGRLSDRFGRKRLLLLGLLLLAVSTLLF 78

                          90       100
                  ....*....|....*....|....*....
gi 2286439362 122 AYLHIPashnkYYMLVARGLLGIGAVFQT 150
Cdd:cd17325    79 AFATSY-----WQLLLARFLQGLASAAVW 102

MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and metabolism, Amino acid transport and metabolism, Inorganic ion transport and metabolism, General function prediction only];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  33 KSRWRSIRILYLTMFLSSVGFSVVMMSIwPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSIL 112
Cdd:COG0477    10 RRRRRALLALALGTFLEGLDFTIVNVAL-PSIAA-DLGASSAQLGWIVSAYLLGRAIGLLLFGRLGDRYGRKRVLLIGLL 87

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2286439362 113 ISVAANCLYAYLHIPAShnkyyMLVARGLLGIGA 146
Cdd:COG0477    88 LFGLASLLCGLAPSPEL-----LIAARALQGIGA 116

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  35 RWRSIRILYLTMFLSSVGFSVVMMSIWPYLQKIDpTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILIS 114
Cdd:cd17325   197 RDRRLLALFLAIFVLAFAFGALEPFLPLYAAELG-GLSPAQIGLLFGAQGLASALSQPPAGKLSDRIGRKPLILIGLLLS 275

                          90       100       110
                  ....*....|....*....|....*....|.
gi 2286439362 115 VAanclyAYLHIPASHNKYYMLVARGLLGIG 145
Cdd:cd17325   276 AV-----ALLLLPLATSFWLLLLLLALLGLG 301

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  44 LTMFLSSVGFSVVMMS-IWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYA 122
Cdd:cd06174     1 LLLGFFLTGLARGLISpLLPALLQ-SFGLSASQLGLLFALFSLGYALLQPLAGLLADRFGRRPVLLLGLLLFALGALLFA 79

                          90       100
                  ....*....|....*....|....*.
gi 2286439362 123 YLHIPAShnkyyMLVARGLLGIGAVF 148
Cdd:cd06174    80 FAPSFWL-----LLLGRFLLGLGSGL 100

Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily domain-containing protein 9 (MFSD9) is expressed in the central nervous system (CNS) and in most peripheral tissues but at very low expression levels. The function of MFSD9 is unknown. MFSD9 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  42 LYLTMFLSSVGFSVVMMSIWPYLQKIDptADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILIsvaanCLY 121
Cdd:cd17390     1 LYLVGFLDLFGVSMILPLLSTHIRSLG--ASPTVIGLIGSVYGALQLFSSPLVGSWSDVVGRRPVLLICLLL-----TAL 73

                          90       100
                  ....*....|....*....|...
gi 2286439362 122 AYLHIPASHNKYYMLVARGLLGI 144
Cdd:cd17390    74 GYVLLGFSTSLALLFLSRIPLGI 96

Sugar phosphate permease [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  33 KSRWRSIRILYLTMFLSSVGFSVVMMsIWPYLQK---IDPTAdtsfLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIV 109
Cdd:COG2271     6 RYRWRILALLFLAYFLNYLDRSNLSV-AAPPLQAdlgLSAAQ----LGLLASAFFLGYALGQIPAGLLADRFGRRRVLAI 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 2286439362 110 SILISVAANCLYAYlhipaSHNKYYMLVARGLLGIGA 146
Cdd:COG2271    81 GLLLWGLATLLFGF-----ATSFWQLLLLRFLLGLGE 112

Fucose permease [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  33 KSRWRSIRILYLTMFLSSVGFSVVMmSIWPYLQKIDpTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSIL 112
Cdd:COG0738     4 KNYRRALIAIYLLFFLWGFATALWG-PLIPALKDAF-GLSDAQAGLLLFAFFLGYLLASLPAGRLIDRFGYKRGLLLGLL 81

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 2286439362 113 ISVAANCLYAYlhipaSHNKYYMLVARGLLGIGAVF 148
Cdd:COG0738    82 LMALGLLLFAL-----APSYPLLLLALFLLGLGLGL 112

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  37 RSIRILYLTMFLSSVGFSVVMMSIWPYLQKIdPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVA 116
Cdd:cd17324   198 PRLRLAYLITFLLFGGFFALYTYLAPFLTDV-PGFSSSAIIGLLLLFGVAGVVGSPLAGRLADRGGRRALLIALLLLAAA 276

                          90       100       110
                  ....*....|....*....|....*....|
gi 2286439362 117 ANCLYAYLHIPAShnkyyMLVARGLLGIGA 146
Cdd:cd17324   277 LLLLTLLGPSPLL-----LLVGLVLWGLGF 301

Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This subfamily is composed of putative multidrug resistance (MDR) transporters including Chlamydia trachomatis antiseptic resistance protein QacA_2, and Serratia sp. DD3 Bmr3. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  37 RSIRILYLTMFLSSVGFSVVMMSIWPYLQkiDPTADTSFL-----GWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSI 111
Cdd:cd17504   177 RNVLLANITALLAGFGMFMVFQLLPQLLQ--LPTPTGFGLgiteaGLLLLPGALVMLIAGPLAGRLIARVGPRRVLLIGL 254

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2286439362 112 LISVAANCLYAYLHIPASHnkyyMLVARGLLGIG 145
Cdd:cd17504   255 VISAVGLLLLALLHSSLWA----LVVALLLIGIG 284

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  42 LYLTMFLSSVGFSVVMMSIWPYLQKIDPTADTSflGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLY 121
Cdd:cd17489     1 ILLANFFFFLSFYLLLPVLPLYAHDLGGSEAVA--GLVVGLFTLAALLARPFAGRLLDRFGRKRLLLIGLLIFALATLLY 78

                          90       100
                  ....*....|....*....|....
gi 2286439362 122 AYLHIPAShnkyyMLVARGLLGIG 145
Cdd:cd17489    79 LLATSVAL-----LLVLRLIHGIG 97

Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator Superfamily; This family is composed of glucose transporters (GLUTs) and other sugar transporters including fungal hexose transporters (HXT), bacterial xylose transporter (XylE), plant sugar transport proteins (STP) and polyol transporters (PLT), H(+)-myo-inositol cotransporter (HMIT), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. The GLUT-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2286439362  72 DTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylhipASHNKYYMLVARGLLGIG 145
Cdd:cd17315    32 STSLQGLVVSSLLLGAAIGSLFGGPLADRFGRRKSLLIAAVLYVIGALLSA-----LAPNVWVLIVGRFLLGLG 100

Metazoan Synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters of the Major Facilitator Superfamily; This family is composed of metazoan synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters including those that transport inorganic phosphate (Pht), aromatic compounds (PcaK and related proteins), proline/betaine (ProP), alpha-ketoglutarate (KgtP), citrate (CitA), shikimate (ShiA), and cis,cis-muconate (MucK), among others. SV2 is a transporter-like protein that serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. Also included in this family is synaptic vesicle 2 (SV2)-related protein (SVOP) and similar proteins. SVOP is a transporter-like nucleotide binding protein that localizes to neurotransmitter-containing vesicles. The SV2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  53 FSVVMMSIWPYLQKIDPTADTsflGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLyaylhIPASHNK 132
Cdd:cd17316    18 IGFVAPVLAAEFSLGLSLLQT---GLLFAAGFLGRPIGALLFGYLGDRIGRKKALILTLLLFGLATLL-----IGLLPTP 89

                          90       100
                  ....*....|....*....|....*..
gi 2286439362 133 YYMLVARGL--LGIGAVFQTCFTFLGE 157
Cdd:cd17316    90 ILLLVLRFLqgIGIGGEYPGASTYVAE 116

Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of bacterial, fungal, and archaeal multidrug resistance (MDR) transporters including several proteins from Bacilli such as methylenomycin A resistance protein (also called MMR peptide), tetracycline resistance protein (TetB), and lincomycin resistance protein LmrB, as well as fungal proteins such as vacuolar basic amino acid transporters, which are involved in the transport into vacuoles of the basic amino acids histidine, lysine, and arginine in Saccharomyces cerevisiae, and aminotriazole/azole resistance proteins. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. For example, MMR confers resistance to the epoxide antibiotic methylenomycin while TetB resistance to tetracycline by an active tetracycline efflux. MMR-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2286439362  37 RSIRILYLTMFLSSVGFSVVMMSIWPYLQKI--DPTADTsflGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILIS 114
Cdd:cd17321   168 RTFSGAVLAAFLLGAALGGLLFLLPLYLQGVlgYSPLQA---GLALLPLALAMLVAAPLAGRLADRFGPRLVLVAGLLLT 244

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2286439362 115 VAANCLYAYLHIPAShnKYYMLVARGLLGIGAVF 148
Cdd:cd17321   245 AVGLLLLALLGADSS--VWLLLPGLVLLGLGLGL 276