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Conserved domains on  [gi|7662316|ref|NP_055518|]
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DDB1- and CUL4-associated factor 1 isoform 1 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1311 1.79e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 66.59  E-value: 1.79e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200   51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200  112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 7662316  1247 RSAQAIHKFDKFNMNISGV-FHPNGLEVIINTE-----IWDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1311
Cdd:cd00200  165 RTGKCVATLTGHTGEVNSVaFSPDGEKLLSSSSdgtikLWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1311 1.79e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 66.59  E-value: 1.79e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200   51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200  112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 7662316  1247 RSAQAIHKFDKFNMNISGV-FHPNGLEVIINTE-----IWDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1311
Cdd:cd00200  165 RTGKCVATLTGHTGEVNSVaFSPDGEKLLSSSSdgtikLWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1307 3.18e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 64.16  E-value: 3.18e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLltsATWSQPLSA-LW---GMKSVFDM 1172
Cdd:COG2319  166 TSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLL---ASGSADGTVrLWdlaTGKLLRTL 242

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 KHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVR 1247
Cdd:COG2319  243 TGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTL----TGHSGGVNSVAFSPDGKLLAsgsDDGTvrLWDLA 318

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 7662316  1248 SAQAIHKFDKFNMNISGV-FHPNGLEVII----NT-EIWDLRTFHLLHTVPAldqcrvvfnHTGTV 1307
Cdd:COG2319  319 TGKLLRTLTGHTGAVRSVaFSPDGKTLASgsddGTvRLWDLATGELLRTLTG---------HTGAV 375
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1311 1.79e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 66.59  E-value: 1.79e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200   51 TGPVRDVAASADGTYLASGSSDKTIRLWDLETGECVRTLTGHTSYVSSVAFSPDGRILSSS------------------- 111

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTL-FNPDLAnnykrNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:cd00200  112 ---------------SRDKTI-------KVWDVETGKCLTTLrGHTDWV-----NSVAFSPDGTFVAsssQDGTikLWDL 164

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 7662316  1247 RSAQAIHKFDKFNMNISGV-FHPNGLEVIINTE-----IWDLRTFHLLHTVPALDQ--CRVVFNHTGTVMYGA 1311
Cdd:cd00200  165 RTGKCVATLTGHTGEVNSVaFSPDGEKLLSSSSdgtikLWDLSTGKCLGTLRGHENgvNSVAFSPDGYLLASG 237
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1307 3.18e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 64.16  E-value: 3.18e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLltsATWSQPLSA-LW---GMKSVFDM 1172
Cdd:COG2319  166 TSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTGAVRSVAFSPDGKLL---ASGSADGTVrLWdlaTGKLLRTL 242

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 KHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVR 1247
Cdd:COG2319  243 TGHSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTL----TGHSGGVNSVAFSPDGKLLAsgsDDGTvrLWDLA 318

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 7662316  1248 SAQAIHKFDKFNMNISGV-FHPNGLEVII----NT-EIWDLRTFHLLHTVPAldqcrvvfnHTGTV 1307
Cdd:COG2319  319 TGKLLRTLTGHTGAVRSVaFSPDGKTLASgsddGTvRLWDLATGELLRTLTG---------HTGAV 375
WD40 COG2319
WD40 repeat [General function prediction only];
1097-1293 4.70e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 4.70e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1097 TCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSAT------WS----QPLSALWG- 1165
Cdd:COG2319  124 RSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHSGAVTSVAFSPDGKLLASGSDdgtvrlWDlatgKLLRTLTGh 203

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1166 MKSVFDmkhsftedhyVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANnykrNCATFNPTDDLVL---NDGV 1242
Cdd:COG2319  204 TGAVRS----------VAFSPDGKLLASGSADGTVRLWDLATGKLLRTLTGHSGSV----RSVAFSPDGRLLAsgsADGT 269

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 7662316  1243 --LWDVRSAQAIHKFDKFNMNISGV-FHPNGLEVIINTE-----IWDLRTFHLLHTVPA 1293
Cdd:COG2319  270 vrLWDLATGELLRTLTGHSGGVNSVaFSPDGKLLASGSDdgtvrLWDLATGKLLRTLTG 328
WD40 COG2319
WD40 repeat [General function prediction only];
1042-1292 5.46e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 5.46e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1042 QRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYN 1121
Cdd:COG2319   27 ALLLLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWD 106

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1122 VFSGQEEASYNCHNSAITHLEPSRDGSLLLTSAT------WS----QPLSALWGmksvfdmkHSFTEDHyVEFSKHSQDR 1191
Cdd:COG2319  107 LATGLLLRTLTGHTGAVRSVAFSPDGKTLASGSAdgtvrlWDlatgKLLRTLTG--------HSGAVTS-VAFSPDGKLL 177

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1192 VIGTKGDIAHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDVRSAQAIHKFDKFNMNISGV- 1265
Cdd:COG2319  178 ASGSDDGTVRLWDLATGKLLRTL----TGHTGAVRSVAFSPDGKLLAsgsADGTvrLWDLATGKLLRTLTGHSGSVRSVa 253

                        250       260       270
                 ....*....|....*....|....*....|..
gi 7662316  1266 FHPNGLEVIINTE-----IWDLRTFHLLHTVP 1292
Cdd:COG2319  254 FSPDGRLLASGSAdgtvrLWDLATGELLRTLT 285
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1093-1307 5.99e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.19  E-value: 5.99e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1093 ESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfdm 1172
Cdd:cd00200   93 TSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHTDWVNSVAFSPDGTFVASS------------------- 153

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1173 khsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGN--KLLTLFNPDLannykrNCATFNPTDDLVL---NDGV--LWD 1245
Cdd:cd00200  154 ---------------SQDGTI-------KLWDLRTGKcvATLTGHTGEV------NSVAFSPDGEKLLsssSDGTikLWD 205

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 7662316  1246 VRSAQAIHKFDKFNMNISGV-FHPNGLEVI-----INTEIWDLRTFHLLHTVPAldqcrvvfnHTGTV 1307
Cdd:cd00200  206 LSTGKCLGTLRGHENGVNSVaFSPDGYLLAsgsedGTIRVWDLRTGECVQTLSG---------HTNSV 264
WD40 COG2319
WD40 repeat [General function prediction only];
1092-1284 1.47e-05

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 49.14  E-value: 1.47e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1092 DESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSatwsqplsalwgmksvfd 1171
Cdd:COG2319  245 HSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHSGGVNSVAFSPDGKLLASG------------------ 306

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1172 mkhsftedhyvefskhSQDRVIgtkgdiaHIYDIQTGNKLLTLfnpdLANNYKRNCATFNPTDDLVL---NDGV--LWDV 1246
Cdd:COG2319  307 ----------------SDDGTV-------RLWDLATGKLLRTL----TGHTGAVRSVAFSPDGKTLAsgsDDGTvrLWDL 359

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
gi 7662316  1247 RSAQAIHKFDKFNMNISGV-FHPNGLEVII----NT-EIWDLRT 1284
Cdd:COG2319  360 ATGELLRTLTGHTGAVTSVaFSPDGRTLASgsadGTvRLWDLAT 403
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 1066-1154 1.70e-04

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 45.40  E-value: 1.70e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 7662316  1066 VDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSR 1145
Cdd:cd00200  150 VASSSQDGTIKLWDLRTGKCVATLTGHTGEVNSVAFSPDGEKLLSSSSDGTIKLWDLSTGKCLGTLRGHENGVNSVAFSP 229


                 ....*....
gi 7662316  1146 DGSLLLTSA 1154
Cdd:cd00200  230 DGYLLASGS 238
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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