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Conserved domains on  [gi|1311721348|ref|NP_215119|]
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IS1536 family serine type transposase [Mycobacterium tuberculosis H37Rv]

Protein Classification

IS607 family transposase( domain architecture ID 16669846)

IS607 family transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Gene Ontology:  GO:0003677
PubMed:  10986230

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
transpos_IS607 super family cl41297
IS607 family transposase;
6-177 1.29e-84

IS607 family transposase;


The actual alignment was detected with superfamily member NF033518:

Pssm-ID: 468054 [Multi-domain]  Cd Length: 187  Bit Score: 247.94  E-value: 1.29e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   6 WAERNGVARVTAYRWFHAGLLPVPA----------RKVGRLILVDELASEAGaqpKTAVYARVSSADQKSDLDRQVARVT 75
Cdd:NF033518    3 AAERLGVSRQTLRRWDREGLIKVPRtpggrrrypeEEVGRLLGVKVPAGDER---KTVIYARVSSADQKDDLERQVARLT 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  76 SWATAEQIPVDKVVTEVGSVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVV--DSAEV 150
Cdd:NF033518   80 QYATAKGWQVDEVVTDIGSGLNEKRKGLLKLLDDIlngEVSRVVVEHKDRLTRFGFEYLEALFKANGVEIVVVneDEKSP 159

                         170       180
                  ....*....|....*....|....*..
gi 1311721348 151 DDDLVWDMTEILTSMCARLYGKRAAQN 177
Cdd:NF033518  160 EEELVEDLIEILTSFSARLYGKRSAKN 186
 
Name Accession Description Interval E-value
transpos_IS607 NF033518
IS607 family transposase;
6-177 1.29e-84

IS607 family transposase;


Pssm-ID: 468054 [Multi-domain]  Cd Length: 187  Bit Score: 247.94  E-value: 1.29e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   6 WAERNGVARVTAYRWFHAGLLPVPA----------RKVGRLILVDELASEAGaqpKTAVYARVSSADQKSDLDRQVARVT 75
Cdd:NF033518    3 AAERLGVSRQTLRRWDREGLIKVPRtpggrrrypeEEVGRLLGVKVPAGDER---KTVIYARVSSADQKDDLERQVARLT 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  76 SWATAEQIPVDKVVTEVGSVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVV--DSAEV 150
Cdd:NF033518   80 QYATAKGWQVDEVVTDIGSGLNEKRKGLLKLLDDIlngEVSRVVVEHKDRLTRFGFEYLEALFKANGVEIVVVneDEKSP 159

                         170       180
                  ....*....|....*....|....*..
gi 1311721348 151 DDDLVWDMTEILTSMCARLYGKRAAQN 177
Cdd:NF033518  160 EEELVEDLIEILTSFSARLYGKRSAKN 186
COG2452 COG2452
Predicted site-specific integrase-resolvase [Mobilome: prophages, transposons];
1-177 1.63e-68

Predicted site-specific integrase-resolvase [Mobilome: prophages, transposons];


Pssm-ID: 441988 [Multi-domain]  Cd Length: 178  Bit Score: 206.77  E-value: 1.63e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   1 MNLAAWAERNGVARVTAYRWFHAGLLPvPARKVGRLILVDELASEAgAQPKTAVYARVSSADQKSDLDRQVARVTSWATA 80
Cdd:COG2452     1 LTPGEAAELLGVSPKTLRRWEKEGKLP-AIRTPGGHRRYPESEVER-LERRTVIYARVSSADQKDDLERQVERLEEYATA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  81 EQIPvDKVVTEVGSVLNGHRRKFPAVLRDLSVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVVDSAEVDDDLVWDMTE 160
Cdd:COG2452    79 KGYQ-DEVIKDVGSGLNYKRKGLLKLLERIEVERVVVEHKDRLARFGFELLEALCEAYGVEIVVVNQESPEQELVEDLIA 157

                         170
                  ....*....|....*..
gi 1311721348 161 ILTSMCARLYGKRAAQN 177
Cdd:COG2452   158 IITSFSARLYGLRSYKK 174
SR_IS607_transposase_like cd03769
Serine Recombinase (SR) family, IS607-like transposase subfamily, catalytic domain; members ...
 52-177 2.03e-47

Serine Recombinase (SR) family, IS607-like transposase subfamily, catalytic domain; members contain a DNA binding domain with homology to MerR/SoxR located N-terminal to the catalytic domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. This subfamily is composed of proteins that catalyze the transposition of insertion sequence (IS) elements such as IS607 from Helicobacter and IS1535 from Mycobacterium, and similar proteins from other bacteria and several archaeal species. IS elements are DNA segments that move to new sites in prokaryotic and eukaryotic genomes causing insertion mutations and gene rearrangements.


Pssm-ID: 239738  Cd Length: 134  Bit Score: 151.65  E-value: 2.03e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  52 TAVYARVSSADQKSDLDRQVARVTSWATaEQIPVDKVVTEVGSVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCRFGS 128
Cdd:cd03769     1 TVLYARVSSHDQKDDLERQIERLEAYAT-AQGWIVEVIKDIGSGLNEKRKGLLKLLEDVlagKVERVVITYKDRLARFGF 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1311721348 129 EYVHAALAAQGRELVVVD---SAEVDDDLVWDMTEILTSMCARLYGKRAAQN 177
Cdd:cd03769    80 ELLEELFKAYGVEIVVINqeeNEELEQELVEDLIEIITSFSARLYGKRSHKK 131
Resolvase smart00857
Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the ...
 52-173 5.17e-14

Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.


Pssm-ID: 214861 [Multi-domain]  Cd Length: 148  Bit Score: 66.11  E-value: 5.17e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   52 TAVYARVSSADQK-SDLDRQVARVTSWATAEQIPVDKVVTEVG-SVLNGHRRKFPAVLRDLS---VTRIVVEHRDRFCRF 126
Cdd:smart00857   1 AAGYARVSTDDQAdGSLERQLEALRAYAERNGWEVVRIYEDEGvSGKKADRPGLQRLLADLRagdIDVLVVYKLDRLGRS 80

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1311721348  127 GSEYVH--AALAAQGRELVVVDSAEVD-----DDLVWDMTEILTSMCARLYGKR 173
Cdd:smart00857  81 LRDLLAllELLEEKGVRLVSLKEGILDtstpaGRLLLDILAALAEFERELISER 134
Resolvase pfam00239
Resolvase, N terminal domain; The N-terminal domain of the resolvase family (this family) ...
 53-167 4.64e-11

Resolvase, N terminal domain; The N-terminal domain of the resolvase family (this family) contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase - see pfam02796.


Pssm-ID: 425548 [Multi-domain]  Cd Length: 144  Bit Score: 58.05  E-value: 4.64e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  53 AVYARVSSADQKSDLDRQVARVTSWAtAEQIPVDKVVTEVGSVLNGHRRKFPAVLRDLSVTR---IVVEHRDRFCRFGSE 129
Cdd:pfam00239   2 IGYARVSTEDQDDSLERQLEALRAYA-ACNGKIVEFEDKGVSGRKLDRPGLQRLLALLRAGKgdvLVVYKLDRLGRSLRD 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1311721348 130 YVHA--ALAAQGRELVVVDSAEVDDDlvwDMTEILTSMCA 167
Cdd:pfam00239  81 LLTLveELREKGVDLVSLDEGIDTST---PMGRLLLTILA 117
 
Name Accession Description Interval E-value
transpos_IS607 NF033518
IS607 family transposase;
6-177 1.29e-84

IS607 family transposase;


Pssm-ID: 468054 [Multi-domain]  Cd Length: 187  Bit Score: 247.94  E-value: 1.29e-84
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   6 WAERNGVARVTAYRWFHAGLLPVPA----------RKVGRLILVDELASEAGaqpKTAVYARVSSADQKSDLDRQVARVT 75
Cdd:NF033518    3 AAERLGVSRQTLRRWDREGLIKVPRtpggrrrypeEEVGRLLGVKVPAGDER---KTVIYARVSSADQKDDLERQVARLT 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  76 SWATAEQIPVDKVVTEVGSVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVV--DSAEV 150
Cdd:NF033518   80 QYATAKGWQVDEVVTDIGSGLNEKRKGLLKLLDDIlngEVSRVVVEHKDRLTRFGFEYLEALFKANGVEIVVVneDEKSP 159

                         170       180
                  ....*....|....*....|....*..
gi 1311721348 151 DDDLVWDMTEILTSMCARLYGKRAAQN 177
Cdd:NF033518  160 EEELVEDLIEILTSFSARLYGKRSAKN 186
COG2452 COG2452
Predicted site-specific integrase-resolvase [Mobilome: prophages, transposons];
1-177 1.63e-68

Predicted site-specific integrase-resolvase [Mobilome: prophages, transposons];


Pssm-ID: 441988 [Multi-domain]  Cd Length: 178  Bit Score: 206.77  E-value: 1.63e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   1 MNLAAWAERNGVARVTAYRWFHAGLLPvPARKVGRLILVDELASEAgAQPKTAVYARVSSADQKSDLDRQVARVTSWATA 80
Cdd:COG2452     1 LTPGEAAELLGVSPKTLRRWEKEGKLP-AIRTPGGHRRYPESEVER-LERRTVIYARVSSADQKDDLERQVERLEEYATA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  81 EQIPvDKVVTEVGSVLNGHRRKFPAVLRDLSVTRIVVEHRDRFCRFGSEYVHAALAAQGRELVVVDSAEVDDDLVWDMTE 160
Cdd:COG2452    79 KGYQ-DEVIKDVGSGLNYKRKGLLKLLERIEVERVVVEHKDRLARFGFELLEALCEAYGVEIVVVNQESPEQELVEDLIA 157

                         170
                  ....*....|....*..
gi 1311721348 161 ILTSMCARLYGKRAAQN 177
Cdd:COG2452   158 IITSFSARLYGLRSYKK 174
SR_IS607_transposase_like cd03769
Serine Recombinase (SR) family, IS607-like transposase subfamily, catalytic domain; members ...
 52-177 2.03e-47

Serine Recombinase (SR) family, IS607-like transposase subfamily, catalytic domain; members contain a DNA binding domain with homology to MerR/SoxR located N-terminal to the catalytic domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. This subfamily is composed of proteins that catalyze the transposition of insertion sequence (IS) elements such as IS607 from Helicobacter and IS1535 from Mycobacterium, and similar proteins from other bacteria and several archaeal species. IS elements are DNA segments that move to new sites in prokaryotic and eukaryotic genomes causing insertion mutations and gene rearrangements.


Pssm-ID: 239738  Cd Length: 134  Bit Score: 151.65  E-value: 2.03e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  52 TAVYARVSSADQKSDLDRQVARVTSWATaEQIPVDKVVTEVGSVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCRFGS 128
Cdd:cd03769     1 TVLYARVSSHDQKDDLERQIERLEAYAT-AQGWIVEVIKDIGSGLNEKRKGLLKLLEDVlagKVERVVITYKDRLARFGF 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1311721348 129 EYVHAALAAQGRELVVVD---SAEVDDDLVWDMTEILTSMCARLYGKRAAQN 177
Cdd:cd03769    80 ELLEELFKAYGVEIVVINqeeNEELEQELVEDLIEIITSFSARLYGKRSHKK 131
Resolvase smart00857
Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the ...
 52-173 5.17e-14

Resolvase, N terminal domain; The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.


Pssm-ID: 214861 [Multi-domain]  Cd Length: 148  Bit Score: 66.11  E-value: 5.17e-14
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348   52 TAVYARVSSADQK-SDLDRQVARVTSWATAEQIPVDKVVTEVG-SVLNGHRRKFPAVLRDLS---VTRIVVEHRDRFCRF 126
Cdd:smart00857   1 AAGYARVSTDDQAdGSLERQLEALRAYAERNGWEVVRIYEDEGvSGKKADRPGLQRLLADLRagdIDVLVVYKLDRLGRS 80

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1311721348  127 GSEYVH--AALAAQGRELVVVDSAEVD-----DDLVWDMTEILTSMCARLYGKR 173
Cdd:smart00857  81 LRDLLAllELLEEKGVRLVSLKEGILDtstpaGRLLLDILAALAEFERELISER 134
Resolvase pfam00239
Resolvase, N terminal domain; The N-terminal domain of the resolvase family (this family) ...
 53-167 4.64e-11

Resolvase, N terminal domain; The N-terminal domain of the resolvase family (this family) contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase - see pfam02796.


Pssm-ID: 425548 [Multi-domain]  Cd Length: 144  Bit Score: 58.05  E-value: 4.64e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  53 AVYARVSSADQKSDLDRQVARVTSWAtAEQIPVDKVVTEVGSVLNGHRRKFPAVLRDLSVTR---IVVEHRDRFCRFGSE 129
Cdd:pfam00239   2 IGYARVSTEDQDDSLERQLEALRAYA-ACNGKIVEFEDKGVSGRKLDRPGLQRLLALLRAGKgdvLVVYKLDRLGRSLRD 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1311721348 130 YVHA--ALAAQGRELVVVDSAEVDDDlvwDMTEILTSMCA 167
Cdd:pfam00239  81 LLTLveELREKGVDLVSLDEGIDTST---PMGRLLLTILA 117
Ser_Recombinase cd00338
Serine Recombinase family, catalytic domain; a DNA binding domain may be present either N- or ...
 54-173 1.27e-10

Serine Recombinase family, catalytic domain; a DNA binding domain may be present either N- or C-terminal to the catalytic domain. These enzymes perform site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and serine recombinase. Serine recombinases demonstrate functional versatility and include resolvases, invertases, integrases, and transposases. Resolvases and invertases (i.e. Tn3, gamma-delta, Tn5044 resolvases, Gin and Hin invertases) in this family contain a C-terminal DNA binding domain and comprise a major phylogenic group. Also included are phage- and bacterial-encoded recombinases such as phiC31 integrase, SpoIVCA excisionase, and Tn4451 TnpX transposase. These integrases and transposases have larger C-terminal domains compared to resolvases/invertases and are referred to as large serine recombinases. Also belonging to this family are proteins with N-terminal DNA binding domains similar to IS607- and IS1535-transposases from Helicobacter and Mycobacterium.


Pssm-ID: 238206 [Multi-domain]  Cd Length: 137  Bit Score: 56.89  E-value: 1.27e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  54 VYARVSSADQKSD--LDRQVARVTSWATAEQIPVDKVVTEVGS--VLNGHRRKFPAVLRDLS---VTRIVVEHRDRFCRF 126
Cdd:cd00338     1 IYARVSTDKQEQGdsLERQREALREYAARNGLEVVGEYEDAGSsaTSLVDRPGLQRLLADVKagkIDVVLVEKLDRLSRN 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 1311721348 127 GSEYVH--AALAAQGRELVVVDSAEVDDDLVWDMTEILTSMCARLYGKR 173
Cdd:cd00338    81 LVDLLEllELLEAHGVRVVTADGEIDLDSEDGRLMLGILAAMAEEESKL 129
SpoIVCA COG1961
Site-specific DNA recombinase SpoIVCA/DNA invertase PinE [Replication, recombination and ...
 51-125 4.42e-08

Site-specific DNA recombinase SpoIVCA/DNA invertase PinE [Replication, recombination and repair];


Pssm-ID: 441564 [Multi-domain]  Cd Length: 388  Bit Score: 51.95  E-value: 4.42e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1311721348  51 KTAVYARVSSADQKS-DLDRQVARVTSWATAEQIPVDKVVTEVG-SVLNGHRRKFPAVLRDL---SVTRIVVEHRDRFCR 125
Cdd:COG1961     3 RAAGYARVSTDDQEGlSLERQREALRAYAEKAGWEIVRIYVDEGvSGTSKDRPGLQRLLADLragKFDTLVVWKLDRLGR 82
SR_ResInv cd03768
Serine Recombinase (SR) family, Resolvase and Invertase subfamily, catalytic domain; members ...
 55-125 1.97e-07

Serine Recombinase (SR) family, Resolvase and Invertase subfamily, catalytic domain; members contain a C-terminal DNA binding domain. Serine recombinases catalyze site-specific recombination of DNA molecules by a concerted, four-strand cleavage and rejoining mechanism which involves a transient phosphoserine linkage between DNA and the enzyme. They are functionally versatile and include resolvases, invertases, integrases, and transposases. Resolvases and invertases affect resolution or inversion and comprise a major phylogenic group. Resolvases (e.g. Tn3, gamma-delta, and Tn5044) normally recombine two sites in direct repeat causing deletion of the DNA between the sites. Invertases (e.g. Gin and Hin) recombine sites in inverted repeat to invert the DNA between the sites. Cointegrate resolution with gamma-delta resolvase requires the formation of a synaptosome of three resolvase dimers bound to each of two res sites on the DNA. Also included in this subfamily are some putative integrases including a sequence from bacteriophage phi-FC1.


Pssm-ID: 239737 [Multi-domain]  Cd Length: 126  Bit Score: 47.86  E-value: 1.97e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1311721348  55 YARVSSADQksDLDRQVARVTSWAtaeqiPVDKVVTEVGSVLNGHRRKFPAVLRDLS-VTRIVVEHRDRFCR 125
Cdd:cd03768     4 YARVSTDDQ--SLERQLEALKAAG-----ECDKIFEEKGSGGKKERPELQKLLEDLReGDTLVVTKLDRLGR 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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