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Conserved domains on  [gi|31542775|ref|NP_775898|]
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endonuclease V isoform 1 [Homo sapiens]

Protein Classification

endonuclease V( domain architecture ID 10158987)

endonuclease V selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-245 3.71e-91

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


:

Pssm-ID: 143472  Cd Length: 208  Bit Score: 268.60  E-value: 3.71e-91
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559   4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559  76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775 177 qtrgDSFPLLGDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQADICSRE 245
Cdd:cd06559 144 ----GSFTPLYDDGEVVGAALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLLSRE 208
 
Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-245 3.71e-91

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


Pssm-ID: 143472  Cd Length: 208  Bit Score: 268.60  E-value: 3.71e-91
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559   4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559  76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775 177 qtrgDSFPLLGDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQADICSRE 245
Cdd:cd06559 144 ----GSFTPLYDDGEVVGAALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLLSRE 208
Endonuclease_5 pfam04493
Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that ...
46-241 5.35e-81

Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents.


Pssm-ID: 427979  Cd Length: 198  Bit Score: 242.74  E-value: 5.35e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775    46 QRVGGVDVSFVKGD-SVRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKepglmPQVLL 124
Cdd:pfam04493  18 RLVAGVDVSFVKDDdGGTAVAAIVVLDYPTLEVVEEAVAVGPVTFPYIPGLLSFRELPALLEALEKLKQK-----PDLLL 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   125 VDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLlqvdglennaLHKEKIRLLQTRGDSFPLLgDSGTVLGMALRSHDRSt 204
Cdd:pfam04493  93 VDGQGIAHPRRLGIASHLGVLLDLPTIGVAKSR----------LVGKFEEPGEERGSYSPLV-DKGEVVGAVLRTRKNV- 160
                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 31542775   205 RPLYISVGHRMSLEAAVRLTC-CCCRFRIPEPVRQADI 241
Cdd:pfam04493 161 KPIYVSPGHRISLDTALELVLrCLRGYRLPEPTRLADL 198
Nfi COG1515
Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];
18-245 6.84e-72

Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];


Pssm-ID: 441124  Cd Length: 221  Bit Score: 220.39  E-value: 6.84e-72
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  18 KREQARLKAHVVdrdteawqRDPAFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:COG1515  14 RALQRELAARVV--------LEDDLGEPRLVAGVDVSYEDGGD-RAVAAIVVLDYPTLEVVERAVARGPVTFPYIPGLLS 84
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLL--QVDGLENNalhkekirl 175
Cdd:COG1515  85 FRELPALLAALEKLKVE-----PDLLLVDGQGIAHPRRLGIASHLGVLLDVPTIGVAKSRLcgEFEEPGEE--------- 150
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 31542775 176 lqtRGDSFPLLgDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLT-CCCCRFRIPEPVRQADICSRE 245
Cdd:COG1515 151 ---RGSWSPLV-DDGEVIGAVLRTRDG-VKPLYVSPGHRISLETALELVlACTPGYRLPEPTRLADRLARR 216
PRK11617 PRK11617
deoxyribonuclease V;
18-240 1.70e-54

deoxyribonuclease V;


Pssm-ID: 236937  Cd Length: 224  Bit Score: 175.89  E-value: 1.70e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   18 KREQARLKAHVVDRDteawqrDPAFSGLQRVGGVDVSFVKGDSVRAcASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:PRK11617   8 RAQQIELASSVIRED------RLDKDPPRLIAGADVGFEQGGEVTR-AAIVVLKYPSLELVEYQVARIATTMPYIPGFLS 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDglennalHKEkirLLQ 177
Cdd:PRK11617  81 FREYPALLAAWEQLSQK-----PDLVFVDGHGIAHPRRLGVASHFGLLVDVPTIGVAKKRLCGK-------FEP---LSE 145
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 31542775  178 TRGDSFPLLgDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCCR-FRIPEPVRQAD 240
Cdd:PRK11617 146 EPGSLQPLM-DKGEQLGWVWRSKAR-CNPLFISTGHRVSLDSALAWVQRCMKgYRLPEPTRWAD 207
 
Name Accession Description Interval E-value
Endonuclease_V cd06559
Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine ...
17-245 3.71e-91

Endonuclease_V, a DNA repair enzyme that initiates repair of nitrosative deaminated purine bases; Endonuclease_V (EndoV) is an enzyme that can initiate repair of all possible deaminated DNA bases. EndoV cleaves the DNA strand containing lesions at the second phosphodiester bond 3' to the lesion using Mg2+ as a cofactor. EndoV homologs are conserved throughout all domains of life from bacteria to humans. EndoV is encoded by the nfi gene and nfi null mutant mice have a phenotype prone to cancer. The ability of endonuclease V to recognize mismatches and abnormal replicative DNA structures suggests that the enzyme plays an important role in DNA metabolism. The details of downstream processing for the EndoV pathway remain unknown.


Pssm-ID: 143472  Cd Length: 208  Bit Score: 268.60  E-value: 3.71e-91
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  17 WKREQARLKAHVVDRDTEawqrdpaFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFL 96
Cdd:cd06559   4 ARKLQEELAKKVVLEDLG-------PGEVRLVAGVDVSYKKDGD-LAVAAAVVLDYPDLEVVETAVAVGEVTFPYIPGLL 75
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  97 AFREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHkekirll 176
Cdd:cd06559  76 AFREGPPLLEALEKLKTK-----PDLLLVDGHGIAHPRRFGLASHLGVLLDLPTIGVAKSLLVGDGEEPGEER------- 143
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775 177 qtrgDSFPLLGDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCC-RFRIPEPVRQADICSRE 245
Cdd:cd06559 144 ----GSFTPLYDDGEVVGAALRTRDG-VKPVYVSPGHRIDLETAVELVLKCCkGYRLPEPTRLADLLSRE 208
Endonuclease_5 pfam04493
Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that ...
46-241 5.35e-81

Endonuclease V; Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents.


Pssm-ID: 427979  Cd Length: 198  Bit Score: 242.74  E-value: 5.35e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775    46 QRVGGVDVSFVKGD-SVRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKepglmPQVLL 124
Cdd:pfam04493  18 RLVAGVDVSFVKDDdGGTAVAAIVVLDYPTLEVVEEAVAVGPVTFPYIPGLLSFRELPALLEALEKLKQK-----PDLLL 92
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   125 VDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLlqvdglennaLHKEKIRLLQTRGDSFPLLgDSGTVLGMALRSHDRSt 204
Cdd:pfam04493  93 VDGQGIAHPRRLGIASHLGVLLDLPTIGVAKSR----------LVGKFEEPGEERGSYSPLV-DKGEVVGAVLRTRKNV- 160
                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 31542775   205 RPLYISVGHRMSLEAAVRLTC-CCCRFRIPEPVRQADI 241
Cdd:pfam04493 161 KPIYVSPGHRISLDTALELVLrCLRGYRLPEPTRLADL 198
Nfi COG1515
Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];
18-245 6.84e-72

Deoxyinosine 3'-endonuclease (endonuclease V) [Replication, recombination and repair];


Pssm-ID: 441124  Cd Length: 221  Bit Score: 220.39  E-value: 6.84e-72
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  18 KREQARLKAHVVdrdteawqRDPAFSGLQRVGGVDVSFVKGDSvRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:COG1515  14 RALQRELAARVV--------LEDDLGEPRLVAGVDVSYEDGGD-RAVAAIVVLDYPTLEVVERAVARGPVTFPYIPGLLS 84
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLL--QVDGLENNalhkekirl 175
Cdd:COG1515  85 FRELPALLAALEKLKVE-----PDLLLVDGQGIAHPRRLGIASHLGVLLDVPTIGVAKSRLcgEFEEPGEE--------- 150
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 31542775 176 lqtRGDSFPLLgDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLT-CCCCRFRIPEPVRQADICSRE 245
Cdd:COG1515 151 ---RGSWSPLV-DDGEVIGAVLRTRDG-VKPLYVSPGHRISLETALELVlACTPGYRLPEPTRLADRLARR 216
PRK11617 PRK11617
deoxyribonuclease V;
18-240 1.70e-54

deoxyribonuclease V;


Pssm-ID: 236937  Cd Length: 224  Bit Score: 175.89  E-value: 1.70e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   18 KREQARLKAHVVDRDteawqrDPAFSGLQRVGGVDVSFVKGDSVRAcASLVVLSFPELEVVYEESRMVSLTAPYVSGFLA 97
Cdd:PRK11617   8 RAQQIELASSVIRED------RLDKDPPRLIAGADVGFEQGGEVTR-AAIVVLKYPSLELVEYQVARIATTMPYIPGFLS 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   98 FREVPFLLELVQQLREKepglmPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDglennalHKEkirLLQ 177
Cdd:PRK11617  81 FREYPALLAAWEQLSQK-----PDLVFVDGHGIAHPRRLGVASHFGLLVDVPTIGVAKKRLCGK-------FEP---LSE 145
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 31542775  178 TRGDSFPLLgDSGTVLGMALRSHDRsTRPLYISVGHRMSLEAAVRLTCCCCR-FRIPEPVRQAD 240
Cdd:PRK11617 146 EPGSLQPLM-DKGEQLGWVWRSKAR-CNPLFISTGHRVSLDSALAWVQRCMKgYRLPEPTRWAD 207
DUF99 pfam01949
Protein of unknown function DUF99; This family includes uncharacterized archaebacterial ...
146-244 1.63e-03

Protein of unknown function DUF99; This family includes uncharacterized archaebacterial proteins. Sequence and structure analysis to identify RNase H-like superfamily members, has clustered this family in endonuclease Clade V, thus suggesting endonuclease activity of these proteins.


Pssm-ID: 426526  Cd Length: 173  Bit Score: 38.23  E-value: 1.63e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775   146 TDLPCVGVAKKLLQVDGLENnALHK------EKIRLLQTRGDSFPLLGDSGTVlgmalrshdrstrplYI-SVGhrMSLE 218
Cdd:pfam01949  82 TGLPVIAVTREEPDLGRIER-ALRKhfpdgeERLELIRKLGRIEVVVTTGGPV---------------YVqAVG--IDPE 143
                          90       100
                  ....*....|....*....|....*..
gi 31542775   219 AAVRLTC-CCCRFRIPEPVRQADICSR 244
Cdd:pfam01949 144 EAARLIRaFTLEGRIPEPLRVAHLIAR 170
COG1628 COG1628
Endonuclease V homolog, UPF0215 family [General function prediction only];
47-248 3.75e-03

Endonuclease V homolog, UPF0215 family [General function prediction only];


Pssm-ID: 441235  Cd Length: 189  Bit Score: 37.46  E-value: 3.75e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775  47 RVGGVDVSFVKGDSVRacaSLVVLsfpeleVVYEESRmvsltapYVSGFlAFREV--------PFLLELVQQLREKEPgl 118
Cdd:COG1628   4 RVLGIDDSPFEGGDGR---STLAG------VVMRGDR-------VIDGV-AFGRItvdgldatDAIIELVDSLKFRPD-- 64
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 31542775 119 mPQVLLVDG------NGV----LHHRgfgvachlgvlTDLPCVGVAKKLLQVDGLENnALHK------EKIRLLQTRGDS 182
Cdd:COG1628  65 -VRVVLLDGitfagfNVVdlerLHEE-----------TGLPVIAVTREEPDGDAIES-ALRKhfpddeERLELYRRLGPR 131
                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 31542775 183 FPLLGDSGTVlgmalrshdrstrplYI-SVGhrMSLEAAVRLtccCCRF----RIPEPVRQADICSREHIR 248
Cdd:COG1628 132 EPVSVTGGTL---------------YVqAVG--ISPEEAAEL---VRAFtlegRIPEPLRVAHLIARAVRR 182
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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