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Conserved domains on  [gi|1125172490|gb|OLB27386|]
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hypothetical protein AUH95_00270 [Nitrospirae bacterium 13_2_20CM_2_63_8]

Protein Classification

iron-sulfur cluster assembly accessory protein( domain architecture ID 10001059)

iron-sulfur cluster assembly accessory protein from the HesB/IscA family is involved in the assembly of iron-sulfur proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-106 3.24e-52

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 159.16  E-value: 3.24e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELL-QEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYV 79
Cdd:COG0316     1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*..
gi 1125172490  80 DSIQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:COG0316    81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-106 3.24e-52

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 159.16  E-value: 3.24e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELL-QEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYV 79
Cdd:COG0316     1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*..
gi 1125172490  80 DSIQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:COG0316    81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 3-106 7.86e-48

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 148.11  E-value: 7.86e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   3 TVSEMAQKKIYELL-QEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVDS 81
Cdd:TIGR00049   1 TLTDSAAKRIKALLaGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 1125172490  82 IQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 2-106 1.97e-42

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 134.67  E-value: 1.97e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEK-DAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVD 80
Cdd:PRK13623   10 LVFTDAAAAKVKELIEEEGnPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                          90       100
                  ....*....|....*....|....*.
gi 1125172490  81 SIQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:PRK13623   90 GLEGSRFVIKNPNAKTTCGCGSSFSI 115
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 2-101 1.15e-15

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 66.52  E-value: 1.15e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEKdaIGLRVYVKGGG-------CH-GYQYGMSFETK-TADDDTIVEAGGVKVIMDSQSAPLL- 71
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDK--KELRLDVDDGGgpyskggCSiGGKFSLVLVDEpDPDYDEVIESNGGPIYVDSYSLPFLd 80

                          90       100       110
                  ....*....|....*....|....*....|
gi 1125172490  72 SGSEVDYVDSIQGSGFAIKNPQAKTTCGCG 101
Cdd:pfam01521  81 EGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 1-102 7.49e-10

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 51.56  E-value: 7.49e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELLQEEKDAIGLRVYVKGGGCHGYQYGMSF-ETKtaDDDTIVEAGGVKVIMD----SQSAPLlsgsE 75
Cdd:NF038090    1 MLEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALdEPK--ENDEVFEQDGLTFVIDkellEQCGPI----K 74

                          90       100
                  ....*....|....*....|....*..
gi 1125172490  76 VDYVDSiqGSGFAIKNPQAKTTCGCGS 102
Cdd:NF038090   75 VDFIDC--RSGFSITSSMPLGGGGCGS 99
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 1-106 3.24e-52

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 159.16  E-value: 3.24e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELL-QEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYV 79
Cdd:COG0316     1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                          90       100
                  ....*....|....*....|....*..
gi 1125172490  80 DSIQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:COG0316    81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 3-106 7.86e-48

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 148.11  E-value: 7.86e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   3 TVSEMAQKKIYELL-QEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVDS 81
Cdd:TIGR00049   1 TLTDSAAKRIKALLaGEGEPNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 1125172490  82 IQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 2-106 1.97e-42

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 134.67  E-value: 1.97e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEK-DAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVD 80
Cdd:PRK13623   10 LVFTDAAAAKVKELIEEEGnPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                          90       100
                  ....*....|....*....|....*.
gi 1125172490  81 SIQGSGFAIKNPQAKTTCGCGSSFSA 106
Cdd:PRK13623   90 GLEGSRFVIKNPNAKTTCGCGSSFSI 115
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 2-104 2.36e-22

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 84.02  E-value: 2.36e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVDS 81
Cdd:PRK09504   18 LTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVRE 97

                          90       100
                  ....*....|....*....|...
gi 1125172490  82 IQGSGFAIKNPQAKTTCGCGSSF 104
Cdd:PRK09504   98 GLNQIFKFHNPKAQNECGCGESF 120
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
2-104 4.33e-22

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 83.00  E-value: 4.33e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEKDAIGLRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYVDS 81
Cdd:PRK09502    3 ITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82

                          90       100
                  ....*....|....*....|...
gi 1125172490  82 IQGSGFAIKNPQAKTTCGCGSSF 104
Cdd:PRK09502   83 GLNEGFKFTNPNVKDECGCGESF 105
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 2-104 1.16e-18

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 75.73  E-value: 1.16e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEKDAIG--LRVYVKGGGCHGYQYGMSFETKTADDDTIVEAGGVKVIMDSQSAPLLSGSEVDYV 79
Cdd:PLN03082   55 VHMTDNCIRRLKELQTSEPSAEDkmLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVDYV 134

                          90       100
                  ....*....|....*....|....*.
gi 1125172490  80 DSIQGSGFAIK-NPQAKTTCGCGSSF 104
Cdd:PLN03082  135 EELIRSAFVVStNPSAVGGCSCKSSF 160
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 2-101 1.15e-15

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 66.52  E-value: 1.15e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   2 ITVSEMAQKKIYELLQEEKdaIGLRVYVKGGG-------CH-GYQYGMSFETK-TADDDTIVEAGGVKVIMDSQSAPLL- 71
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDK--KELRLDVDDGGgpyskggCSiGGKFSLVLVDEpDPDYDEVIESNGGPIYVDSYSLPFLd 80

                          90       100       110
                  ....*....|....*....|....*....|
gi 1125172490  72 SGSEVDYVDSIQGSGFAIKNPQAKTTCGCG 101
Cdd:pfam01521  81 EGLTLDFVEDLGTLGLKSDNGNLDGNVGCG 110
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
1-95 4.79e-10

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 53.87  E-value: 4.79e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELLQEEKDAIGLRVYVKGGGCHGYQYGMSFETKTA--DDDTIVEAGGVKVIMDSQSAPLLSGSEVDY 78
Cdd:PRK11190    1 MITISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYCPPDAveATDTELKFDGFSAYVDELSAPFLEDAEIDF 80

                          90
                  ....*....|....*..
gi 1125172490  79 VDSIQGSGFAIKNPQAK 95
Cdd:PRK11190   81 VTDQLGSQLTLKAPNAK 97
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 1-102 7.49e-10

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 51.56  E-value: 7.49e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1125172490   1 MITVSEMAQKKIYELLQEEKDAIGLRVYVKGGGCHGYQYGMSF-ETKtaDDDTIVEAGGVKVIMD----SQSAPLlsgsE 75
Cdd:NF038090    1 MLEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALdEPK--ENDEVFEQDGLTFVIDkellEQCGPI----K 74

                          90       100
                  ....*....|....*....|....*..
gi 1125172490  76 VDYVDSiqGSGFAIKNPQAKTTCGCGS 102
Cdd:NF038090   75 VDFIDC--RSGFSITSSMPLGGGGCGS 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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