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Conserved domains on  [gi|1176135568|gb|OQX54703|]
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MAG: type IV pili twitching motility protein PilT, partial [Candidatus Omnitrophica bacterium 4484_213]

Protein Classification

type IV pilus twitching motility protein PilT( domain architecture ID 10006640)

type IV pilus twitching motility protein PilT is a hexameric ATPase that drives pilus retraction, which provides the force required for travel of bacteria in low water environments by a mechanism known as twitching motility

CATH:  3.30.450.90
Gene Ontology:  GO:0005524|GO:0043107
PubMed:  12142488
SCOP:  4004005

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PilT COG2805
Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular ...
 1-132 4.99e-83

Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular structures];


:

Pssm-ID: 442056  Cd Length: 342  Bit Score: 246.93  E-value: 4.99e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:COG2805   209 DLETIEAALTAAETGHLVFATLHTNSAAQTIDRIIDVFPPEEQAQIRSQLAESLRGVISQRLLPRADGGGRVAAREILVN 288

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSK 132
Cdd:COG2805   289 TPAVRNLIREGKTHQIPSLIQTGKKLGMQTMDQSLAELVKEGLITEETALAY 340
 
Name Accession Description Interval E-value
PilT COG2805
Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular ...
 1-132 4.99e-83

Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular structures];


Pssm-ID: 442056  Cd Length: 342  Bit Score: 246.93  E-value: 4.99e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:COG2805   209 DLETIEAALTAAETGHLVFATLHTNSAAQTIDRIIDVFPPEEQAQIRSQLAESLRGVISQRLLPRADGGGRVAAREILVN 288

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSK 132
Cdd:COG2805   289 TPAVRNLIREGKTHQIPSLIQTGKKLGMQTMDQSLAELVKEGLITEETALAY 340
pilT_fam TIGR01420
pilus retraction protein PilT; This model represents the PilT subfamily of proteins related to ...
 1-138 1.50e-69

pilus retraction protein PilT; This model represents the PilT subfamily of proteins related to GspE, a protein involved in type II secretion (also called the General Secretion Pathway). PilT is an apparent cytosolic ATPase associated with type IV pilus systems. It is not required for pilin biogenesis, but is required for twitching motility and social gliding behaviors, shown in some species, powered by pilus retraction. Members of this family may be found in some species that type IV pili but have related structures for DNA uptake and natural transformation. [Cell envelope, Surface structures, Cellular processes, Chemotaxis and motility]


Pssm-ID: 273613  Cd Length: 343  Bit Score: 212.57  E-value: 1.50e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:TIGR01420 206 DLETVELALTAAETGHLVFGTLHTNSAAQTIERIIDVFPAEEQEQIRTQLAESLVAIISQRLLPKADGGGRVLAVEILIN 285

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSKVKNPEE 138
Cdd:TIGR01420 286 TPAVRNLIREGKTHQIKSLIQTGQQLGMQTFDQHLAQLYKKGLITLEDALEAAPSPHE 343
PilT cd01131
Pilus retraction ATPase PilT; Pilus retraction ATPase PilT is a nucleotide-binding protein ...
 1-119 1.81e-68

Pilus retraction ATPase PilT; Pilus retraction ATPase PilT is a nucleotide-binding protein responsible for the retraction of type IV pili, likely by pili disassembly. This retraction provides the force required for travel of bacteria in low water environments by a mechanism known as twitching motility.


Pssm-ID: 410875 [Multi-domain]  Cd Length: 223  Bit Score: 205.85  E-value: 1.81e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:cd01131   105 DLETIELALTAAETGHLVFSTLHTNSAAQTIDRIIDVFPPEQQEQVRIQLASSLRGVISQRLLPKKDGGGRVAAFEILIN 184

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELY 119
Cdd:cd01131   185 TPAIRNLIREGKTHQIPSLIQTGARDGMQTMDQSLYELY 223
T2SSE pfam00437
Type II/IV secretion system protein; This family contains components of both the Type II ...
 1-66 2.45e-11

Type II/IV secretion system protein; This family contains components of both the Type II protein secretion system (T2SS), including Type 4 pilus (T4P), and Type IV protein secretion system (T4SS) from Gram-negative bacteria. VirB11 ATPase is a subunit of the Agrobacterium tumefaciens transfer DNA (T-DNA) transfer system, a type IV secretion pathway required for delivery of T-DNA and effector proteins to plant cells during infection. The cytoplasmic T2S E ATPase is a Zn-containing protein thought to provide the mechanical force for the secretion process. T2S-E contains Walker A and B motifs, that are essential for secretion and ATPase activity. ATPase PulE and XcpR from Klebsiella oxytoca and Pseudomonas aeruginosa respectively are required for protein secretion via the T2SS. ATPase PilB is required for T4P extension.


Pssm-ID: 425681 [Multi-domain]  Cd Length: 269  Bit Score: 59.22  E-value: 2.45e-11
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIID-VFPPYqqqtikvQLAGSLKGIISQQLLPRL 66
Cdd:pfam00437 210 DLETAEIALQAANTGHLVLSTLHTNSAAGALTRLQDmGVPPF-------ELASSLLLVIAQRLVRKL 269
PRK10436 PRK10436
hypothetical protein; Provisional
 1-66 9.63e-09

hypothetical protein; Provisional


Pssm-ID: 236694  Cd Length: 462  Bit Score: 52.24  E-value: 9.63e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIidvfppyQQQTI-KVQLAGSLKGIISQQLLPRL 66
Cdd:PRK10436  298 DGETAEIAIKAAQTGHLVLSTLHTNSTSETLVRL-------QQMGIaRWMLASALKLVIAQRLVRKL 357
 
Name Accession Description Interval E-value
PilT COG2805
Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular ...
 1-132 4.99e-83

Type IV pilus assembly protein PilT, pilus retraction ATPase [Cell motility, Extracellular structures];


Pssm-ID: 442056  Cd Length: 342  Bit Score: 246.93  E-value: 4.99e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:COG2805   209 DLETIEAALTAAETGHLVFATLHTNSAAQTIDRIIDVFPPEEQAQIRSQLAESLRGVISQRLLPRADGGGRVAAREILVN 288

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSK 132
Cdd:COG2805   289 TPAVRNLIREGKTHQIPSLIQTGKKLGMQTMDQSLAELVKEGLITEETALAY 340
pilT_fam TIGR01420
pilus retraction protein PilT; This model represents the PilT subfamily of proteins related to ...
 1-138 1.50e-69

pilus retraction protein PilT; This model represents the PilT subfamily of proteins related to GspE, a protein involved in type II secretion (also called the General Secretion Pathway). PilT is an apparent cytosolic ATPase associated with type IV pilus systems. It is not required for pilin biogenesis, but is required for twitching motility and social gliding behaviors, shown in some species, powered by pilus retraction. Members of this family may be found in some species that type IV pili but have related structures for DNA uptake and natural transformation. [Cell envelope, Surface structures, Cellular processes, Chemotaxis and motility]


Pssm-ID: 273613  Cd Length: 343  Bit Score: 212.57  E-value: 1.50e-69
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:TIGR01420 206 DLETVELALTAAETGHLVFGTLHTNSAAQTIERIIDVFPAEEQEQIRTQLAESLVAIISQRLLPKADGGGRVLAVEILIN 285

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSKVKNPEE 138
Cdd:TIGR01420 286 TPAVRNLIREGKTHQIKSLIQTGQQLGMQTFDQHLAQLYKKGLITLEDALEAAPSPHE 343
PilT cd01131
Pilus retraction ATPase PilT; Pilus retraction ATPase PilT is a nucleotide-binding protein ...
 1-119 1.81e-68

Pilus retraction ATPase PilT; Pilus retraction ATPase PilT is a nucleotide-binding protein responsible for the retraction of type IV pili, likely by pili disassembly. This retraction provides the force required for travel of bacteria in low water environments by a mechanism known as twitching motility.


Pssm-ID: 410875 [Multi-domain]  Cd Length: 223  Bit Score: 205.85  E-value: 1.81e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:cd01131   105 DLETIELALTAAETGHLVFSTLHTNSAAQTIDRIIDVFPPEQQEQVRIQLASSLRGVISQRLLPKKDGGGRVAAFEILIN 184

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELY 119
Cdd:cd01131   185 TPAIRNLIREGKTHQIPSLIQTGARDGMQTMDQSLYELY 223
PilU COG5008
Type IV pilus assembly protein, ATPase PilU [Cell motility, Extracellular structures];
1-140 6.45e-44

Type IV pilus assembly protein, ATPase PilU [Cell motility, Extracellular structures];


Pssm-ID: 444032  Cd Length: 370  Bit Score: 147.55  E-value: 6.45e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGeGRVVATEILIN 80
Cdd:COG5008   207 DRETMEHAIAFAETGHLCLATLHANNANQALDRIINFFPEERRPQLLMDLSLNLRAIVSQRLVPTKDG-GRVAAVEVLLN 285

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568  81 TPAVGNLIREHKTEQIPTVIQTGSKLGMKSMDKSLKELYKKGIISYEVAMSKVKNPEEFR 140
Cdd:COG5008   286 TPLIADLIRKGEIHEIKEAMEKSRELGMQTFDQALFRLYEAGKISYEEALRNADSANNLR 345
DotB_TraJ cd19516
dot/icm secretion system protein DotB-like; Defect in organelle trafficking (Dot)B is part of ...
 1-79 3.84e-18

dot/icm secretion system protein DotB-like; Defect in organelle trafficking (Dot)B is part of the type IVb secretion (T4bS) system, also known as the dot/icm system, and is the main energy supplier of the secretion system. It is an ATPase, similar to the VirB11 component of the T4aS systems. This family also includes Escherichia coli IncI plasmid-encoded conjugative transfer ATPase TraJ encoded on the tra (transfer) operon.


Pssm-ID: 410924 [Multi-domain]  Cd Length: 179  Bit Score: 76.26  E-value: 3.84e-18
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGeGRVVATEILI 79
Cdd:cd19516   100 DQETISAAVEASLTGHPVYSTVHTKSVAETIRRLISLFPPEERDAAAYDLLSTLRFIIVQRLVRTTDG-KRVAVREYLV 177
PulE COG2804
Type II secretory pathway ATPase GspE/PulE or T4P pilus assembly pathway ATPase PilB [Cell ...
 1-127 7.56e-18

Type II secretory pathway ATPase GspE/PulE or T4P pilus assembly pathway ATPase PilB [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 442055 [Multi-domain]  Cd Length: 561  Bit Score: 78.70  E-value: 7.56e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDV-FPPYqqqtikvQLAGSLKGIISQQLLPRL------------- 66
Cdd:COG2804   393 DLETAEIAVQAALTGHLVLSTLHTNDAPSAITRLLDMgVEPF-------LLASSLLGVLAQRLVRRLcphckepyepdpe 465

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568  67 --------------------------NGEG---RVVATEILINTPAVGNLIREHKTEQipTVIQTGSKLGMKSMDKSLKE 117
Cdd:COG2804   466 elerlglppeelapltfyrgvgcehcNGTGykgRTGIYELLVIDDELRELIAEGASAA--ELREAARKEGMRTLREDGLE 543

                         170
                  ....*....|
gi 1176135568 118 LYKKGIISYE 127
Cdd:COG2804   544 KVLQGITTLE 553
PulE-GspE-like cd01129
PulE-GspE family; PulE and General secretory pathway protein GspE are ATPases of the type II ...
 1-79 9.97e-18

PulE-GspE family; PulE and General secretory pathway protein GspE are ATPases of the type II secretory pathway, the main terminal branch of the general secretory pathway (GSP). PulE is a cytoplasmic protein of the GSP, which contains an ATP binding site and a tetracysteine motif. This subgroup also includes PilB, a type IV pilus assembly ATPase, DotB, an ATPase of the type IVb secretion system, also known as the dot/icm system, Escherichia coli IncI plasmid-encoded conjugative transfer ATPase TraJ, and HofB.


Pssm-ID: 410873 [Multi-domain]  Cd Length: 159  Bit Score: 74.44  E-value: 9.97e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVF-PPYqqqtikvQLAGSLKGIISQQLLprlngeGRVVATEILI 79
Cdd:cd01129    91 DAETAEIAIRAALTGHLVLSTLHTNDALGAITRLLDMGiEPF-------LLASALRGVIAQRLV------GRTAIAEVLV 157
T2SSE pfam00437
Type II/IV secretion system protein; This family contains components of both the Type II ...
 1-66 2.45e-11

Type II/IV secretion system protein; This family contains components of both the Type II protein secretion system (T2SS), including Type 4 pilus (T4P), and Type IV protein secretion system (T4SS) from Gram-negative bacteria. VirB11 ATPase is a subunit of the Agrobacterium tumefaciens transfer DNA (T-DNA) transfer system, a type IV secretion pathway required for delivery of T-DNA and effector proteins to plant cells during infection. The cytoplasmic T2S E ATPase is a Zn-containing protein thought to provide the mechanical force for the secretion process. T2S-E contains Walker A and B motifs, that are essential for secretion and ATPase activity. ATPase PulE and XcpR from Klebsiella oxytoca and Pseudomonas aeruginosa respectively are required for protein secretion via the T2SS. ATPase PilB is required for T4P extension.


Pssm-ID: 425681 [Multi-domain]  Cd Length: 269  Bit Score: 59.22  E-value: 2.45e-11
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIID-VFPPYqqqtikvQLAGSLKGIISQQLLPRL 66
Cdd:pfam00437 210 DLETAEIALQAANTGHLVLSTLHTNSAAGALTRLQDmGVPPF-------ELASSLLLVIAQRLVRKL 269
plasmid_TraJ TIGR02525
plasmid transfer ATPase TraJ; Members of this protein family are predicted ATPases associated ...
 1-80 2.15e-09

plasmid transfer ATPase TraJ; Members of this protein family are predicted ATPases associated with plasmid transfer loci in bacteria. This family is most similar to the DotB ATPase of a type-IV secretion-like system of obligate intracellular pathogens Legionella pneumophila and Coxiella burnetii (TIGR02524). [Mobile and extrachromosomal element functions, Plasmid functions]


Pssm-ID: 131577 [Multi-domain]  Cd Length: 372  Bit Score: 54.43  E-value: 2.15e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIIDVFPPYQQQTIKVQLAGSLKGIISQQLLPRLNGEGRVVATEILIN 80
Cdd:TIGR02525 236 DLETFQAAVLAGQSGHFCLGTLHVKSPGEAISRCLQMYPPEMREAAAFDLLSILQYIIVQRLLRTTDGKRQAVREYIVFD 315
PRK10436 PRK10436
hypothetical protein; Provisional
 1-66 9.63e-09

hypothetical protein; Provisional


Pssm-ID: 236694  Cd Length: 462  Bit Score: 52.24  E-value: 9.63e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1176135568   1 DLETISLALTAAETGHLVLATLHTPDAAQTIDRIidvfppyQQQTI-KVQLAGSLKGIISQQLLPRL 66
Cdd:PRK10436  298 DGETAEIAIKAAQTGHLVLSTLHTNSTSETLVRL-------QQMGIaRWMLASALKLVIAQRLVRKL 357
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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