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Conserved domains on  [gi|1276961822|gb|PIP76079|]
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hypothetical protein COW86_00170 [Candidatus Kuenenbacteria bacterium CG22_combo_CG10-13_8_21_14_all_39_9]

Protein Classification

DUF167 domain-containing protein( domain architecture ID 10004820)

uncharacterized DUF167 family protein similar to Escherichia coli YggU

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YggU COG1872
Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];
1-70 9.66e-26

Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];


:

Pssm-ID: 441476  Cd Length: 81  Bit Score: 89.77  E-value: 9.66e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1276961822  1 MIIKVKVTPKANVNKVI-RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:COG1872   11 LILAVRVQPGASRDAIVgVHGGALKVRVTAPPVDGKANAALIKLLAKALGVPKSRVEIVSGETSRNKRVRI 81
 
Name Accession Description Interval E-value
YggU COG1872
Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];
1-70 9.66e-26

Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];


Pssm-ID: 441476  Cd Length: 81  Bit Score: 89.77  E-value: 9.66e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1276961822  1 MIIKVKVTPKANVNKVI-RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:COG1872   11 LILAVRVQPGASRDAIVgVHGGALKVRVTAPPVDGKANAALIKLLAKALGVPKSRVEIVSGETSRNKRVRI 81
DUF167 pfam02594
Uncharacterized ACR, YggU family COG1872;
2-71 7.71e-21

Uncharacterized ACR, YggU family COG1872;


Pssm-ID: 460608  Cd Length: 75  Bit Score: 77.07  E-value: 7.71e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1276961822  2 IIKVKVTPKANVNKVI---RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEIS 71
Cdd:pfam02594  3 ILAVRVKPGAKRDAIGggaVGRGALKVRVAAPPVDGKANAALIEFLAKALGVPKSDVSIVSGETSRHKVVRIE 75
PRK00647 PRK00647
hypothetical protein; Validated
 2-66 1.74e-17

hypothetical protein; Validated


Pssm-ID: 100624  Cd Length: 96  Bit Score: 69.51  E-value: 1.74e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1276961822  2 IIKVKVTPKANVNKVIRKDDQ-YHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDK 66
Cdd:PRK00647   7 ILEVKVTPKARENKIVGFEGGiLKVRVTEVPEKGKANDAVIALLAKFLSLPKRDVTLIAGETSRKK 72
TIGR00251 TIGR00251
TIGR00251 family protein; [Hypothetical proteins, Conserved]
 1-70 2.52e-03

TIGR00251 family protein; [Hypothetical proteins, Conserved]


Pssm-ID: 129353  Cd Length: 87  Bit Score: 33.25  E-value: 2.52e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1276961822  1 MIIKVKVTPKANVNKVIRKD---DQYHVYTTFPPDKGKANQAVIKLLSKelsIPKSKISIIKGEKSRDKIIEI 70
Cdd:TIGR00251  8 LLIRIYVQPKASKDSIVGYNewrKRVEVKIKAPPVEGKANRELIKFFGE---IFGVDVEIVSGELSRQKTIKI 77
GH32_EcAec43-like cd08995
Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 ...
 16-40 7.11e-03

Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 (FosGH2); This glycosyl hydrolase family 32 (GH32) subgroup includes Escherichia coli strain BEN2908 putative glycoside hydrolase Aec43 (FosGH2). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH32 family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize.


Pssm-ID: 350109 [Multi-domain]  Cd Length: 281  Bit Score: 32.93  E-value: 7.11e-03
                          10        20
                  ....*....|....*....|....*.
gi 1276961822  16 VIRKDDQYHV-YTTFPPDKGKANQAV 40
Cdd:cd08995    67 VIKDDGTYHAfYTGHNPDFGKPKQVI 92
 
Name Accession Description Interval E-value
YggU COG1872
Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];
1-70 9.66e-26

Uncharacterized conserved protein YggU, UPF0235/DUF167 family [Function unknown];


Pssm-ID: 441476  Cd Length: 81  Bit Score: 89.77  E-value: 9.66e-26
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1276961822  1 MIIKVKVTPKANVNKVI-RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:COG1872   11 LILAVRVQPGASRDAIVgVHGGALKVRVTAPPVDGKANAALIKLLAKALGVPKSRVEIVSGETSRNKRVRI 81
DUF167 pfam02594
Uncharacterized ACR, YggU family COG1872;
2-71 7.71e-21

Uncharacterized ACR, YggU family COG1872;


Pssm-ID: 460608  Cd Length: 75  Bit Score: 77.07  E-value: 7.71e-21
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1276961822  2 IIKVKVTPKANVNKVI---RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEIS 71
Cdd:pfam02594  3 ILAVRVKPGAKRDAIGggaVGRGALKVRVAAPPVDGKANAALIEFLAKALGVPKSDVSIVSGETSRHKVVRIE 75
PRK00647 PRK00647
hypothetical protein; Validated
 2-66 1.74e-17

hypothetical protein; Validated


Pssm-ID: 100624  Cd Length: 96  Bit Score: 69.51  E-value: 1.74e-17
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1276961822  2 IIKVKVTPKANVNKVIRKDDQ-YHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDK 66
Cdd:PRK00647   7 ILEVKVTPKARENKIVGFEGGiLKVRVTEVPEKGKANDAVIALLAKFLSLPKRDVTLIAGETSRKK 72
PRK01310 PRK01310
hypothetical protein; Validated
 31-70 2.58e-13

hypothetical protein; Validated


Pssm-ID: 167208  Cd Length: 104  Bit Score: 58.90  E-value: 2.58e-13
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 1276961822  31 PDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:PRK01310   48 PEGGEANRALIELLAKALGVPKSSVRLLSGATSRLKQLRI 87
PRK05090 PRK05090
hypothetical protein; Validated
 1-70 6.15e-10

hypothetical protein; Validated


Pssm-ID: 179931  Cd Length: 95  Bit Score: 50.31  E-value: 6.15e-10
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1276961822  1 MIIKVKVTPKANVNKVI-RKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:PRK05090  11 LVLRLYIQPKASRDQIVgLHGDELKVAITAPPVDGQANAHLLKFLAKQFRVAKSQVVIEKGELGRHKQVRI 81
PRK04021 PRK04021
hypothetical protein; Reviewed
 2-66 5.83e-06

hypothetical protein; Reviewed


Pssm-ID: 167678  Cd Length: 92  Bit Score: 40.18  E-value: 5.83e-06
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1276961822  2 IIKVKVTPKANVNKVIRKDD---QYHVYTTFPPDKGKANQAVIKLLSKELSipkSKISIIKGEKSRDK 66
Cdd:PRK04021  10 ILQVYVQPKAKENEIEGVDEwrgRLKVKIKAPPVKGKANKELVKFFSKLLG---AEVEIIRGETSREK 74
PRK01530 PRK01530
hypothetical protein; Reviewed
 2-70 1.96e-04

hypothetical protein; Reviewed


Pssm-ID: 134567  Cd Length: 105  Bit Score: 36.33  E-value: 1.96e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1276961822   2 IIKVKVTPKANVNKV-----IRKDDQYHVYTTFPPDKGKANQAVIKLLSKELSIPKSKISIIKGEKSRDKIIEI 70
Cdd:PRK01530   15 LLNLKVKPNAKQNLIsnfviINNIPYLKLSIKAIPEQGKANEEIINYLAKEWKLSRSNIEIIKGHTHSLKTILI 88
TIGR00251 TIGR00251
TIGR00251 family protein; [Hypothetical proteins, Conserved]
 1-70 2.52e-03

TIGR00251 family protein; [Hypothetical proteins, Conserved]


Pssm-ID: 129353  Cd Length: 87  Bit Score: 33.25  E-value: 2.52e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1276961822  1 MIIKVKVTPKANVNKVIRKD---DQYHVYTTFPPDKGKANQAVIKLLSKelsIPKSKISIIKGEKSRDKIIEI 70
Cdd:TIGR00251  8 LLIRIYVQPKASKDSIVGYNewrKRVEVKIKAPPVEGKANRELIKFFGE---IFGVDVEIVSGELSRQKTIKI 77
GH32_EcAec43-like cd08995
Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 ...
 16-40 7.11e-03

Glycosyl hydrolase family 32, such as the putative glycoside hydrolase Escherichia coli Aec43 (FosGH2); This glycosyl hydrolase family 32 (GH32) subgroup includes Escherichia coli strain BEN2908 putative glycoside hydrolase Aec43 (FosGH2). GH32 enzymes cleave sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). GH32 family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize.


Pssm-ID: 350109 [Multi-domain]  Cd Length: 281  Bit Score: 32.93  E-value: 7.11e-03
                          10        20
                  ....*....|....*....|....*.
gi 1276961822  16 VIRKDDQYHV-YTTFPPDKGKANQAV 40
Cdd:cd08995    67 VIKDDGTYHAfYTGHNPDFGKPKQVI 92
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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