|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07188 |
PRK07188 |
nicotinate phosphoribosyltransferase; Provisional |
15-367 |
0e+00 |
|
nicotinate phosphoribosyltransferase; Provisional
Pssm-ID: 235953 [Multi-domain] Cd Length: 352 Bit Score: 613.49 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 15 RLTNKTFKFDERIETGWFSAVYFLKTKEIAKQKRPNNEVVMQFFQK-DHAVLCGTDEAIALIHTFADQSDQLEIFSLKDG 93
Cdd:PRK07188 1 RLTNKTFKFDERIGEGWYSAVYFLKTREIIEKFNPNNIVTMQFFQRrENAVLCGTDEVIALLKTFAKDPSKLKIRYLKDG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 94 DKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAGDGYAAFIGG 173
Cdd:PRK07188 81 DIINPFETVLEIEGPYENFGFLEGIIDGILARRTSVATNAYNVVQAAN----EKPVIFMGDRADHYLQQAGDGYAAYIGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 174 STAQATHAMNEWWGRTGIGTMPHALIQLFEGDVVEAAKAYRDVYPEDELTVLVDYNNDVITDSLKVAHEFKDELKGVRLD 253
Cdd:PRK07188 157 ISAFSTHAQNEWWGKSGFGTMPHALIQMFNGDVVEACKAYHKTFPEDELIALVDYNNDVITDSLKVAREFGDKLKGVRVD 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 254 TSGNMVDKYFLDNQHLMGSFDPRGVNPELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNVPVDIYGVGRSLLDI 333
Cdd:PRK07188 237 TSKNMIDKYFIRHPEVLGTFDPRGVNPELIKALRKALDENGGKHVKIIVSSGFDAKKIREFEAQNVPVDIYGVGSSLLKI 316
|
330 340 350
....*....|....*....|....*....|....
gi 1397887279 334 KVGFTGDSVILDGKPQSKFGRQYNPNPRLEKVSY 367
Cdd:PRK07188 317 NIGFTGDAVELNGKKEAKAGRKYRPNPRLERVKL 350
|
|
| NAPRTase_B |
cd01571 |
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup B. Nicotinate ... |
30-354 |
8.62e-138 |
|
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup B. Nicotinate phosphoribosyltransferase catalyses the formation of NAMN and PPi from 5-phosphoribosy -1-pyrophosphate (PRPP) and nicotinic acid, this is the first, and also rate limiting, reaction in the NAD salvage synthesis. This salvage pathway serves to recycle NAD degradation products.
Pssm-ID: 238805 [Multi-domain] Cd Length: 302 Bit Score: 394.33 E-value: 8.62e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 30 GWFSAVYFLKTKEIAKQKRPNNEVVMQFFQKDH--AVLCGTDEAIALIHTFadqsdQLEIFSLKDGDKISPFETVLTIKG 107
Cdd:cd01571 1 GRFTDVYFLRTRKILEKKGPNPTVTMEFTQRSLpwAVLCGLEEVLALLEGL-----PVKVYALPEGTIFNPKEPVLRIEG 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 108 PYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAGDGYAAFIGGSTAQATHAMNEWWG 187
Cdd:cd01571 76 PYQDFGELETAILGILARASSIATNAARVKLAAG----DKPVISFGDRRDHPAIQPMDGRAAYIGGCDGVSTVLGAELLG 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 188 RTGIGTMPHALIQLFEGDVVEAAKAYRDVYPED-ELTVLVDYNNDVITDSLKVAHEFKDELKGVRLDTSGNmvdkyfldn 266
Cdd:cd01571 152 EKPSGTMPHALIQIFGGDQVEAWKAFDETYPEDvPRIALIDTFNDEKEEALKAAKALGDKLDGVRLDTPSS--------- 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 267 qhlmgsfdPRGVNPELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNvpVDIYGVGRSLLDIK-VGFTGDSVILD 345
Cdd:cd01571 223 --------RRGVFRYLIREVRWALDIRGYKHVKIFVSGGLDEEDIKELEDVG--VDAFGVGTAISKAPpVDFTMDIVEVN 292
|
....*....
gi 1397887279 346 GKPQSKFGR 354
Cdd:cd01571 293 GQPIAKRGK 301
|
|
| PncB |
COG1488 |
Nicotinic acid phosphoribosyltransferase [Coenzyme transport and metabolism]; Nicotinic acid ... |
85-331 |
5.53e-33 |
|
Nicotinic acid phosphoribosyltransferase [Coenzyme transport and metabolism]; Nicotinic acid phosphoribosyltransferase is part of the Pathway/BioSystem: NAD biosynthesis
Pssm-ID: 441097 [Multi-domain] Cd Length: 445 Bit Score: 127.57 E-value: 5.53e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 85 LEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAG 164
Cdd:COG1488 91 GDIRAVPEGTVVFPGEPLLTVEGTLPEAQLLETLLLNILNYPTLIATKAARIVQAAG----GRPLHDFGLRRAHGLEAAV 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 165 DG-YAAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVvEAAKAYRDVYPEDeLTVLVD-YN-NDVITDSLKVAH 241
Cdd:COG1488 167 AAaRAAYLAGFAGTSNVLAGRYYGIPSVGTMAHSFVQAHDDEL-EAFRAFAEAYPDG-TTLLVDtYDtLSGVPNAIELAK 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 242 EF--KDELKGVRLDtSGNMVDkyfldnqhlmgsfdprgvnpeLVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNV 319
Cdd:COG1488 245 ELlaRGGLLGIRLD-SGDLAY---------------------LSKQARELLDEAGLPDVRIIASDDLDEYRIAALLEQGA 302
|
250
....*....|..
gi 1397887279 320 PVDIYGVGRSLL 331
Cdd:COG1488 303 PIDVFGVGTRLV 314
|
|
| NAPRTase_put |
TIGR01513 |
putative nicotinate phosphoribosyltransferase; A deep split separates two related families of ... |
68-367 |
4.26e-32 |
|
putative nicotinate phosphoribosyltransferase; A deep split separates two related families of proteins, one of which includes experimentally characterized examples of nicotinate phosphoribosyltransferase, an the first enzyme of NAD salvage biosynthesis. This model represents the other family. Members have a different (longer) spacing of several key motifs and have an additional C-terminal domain of up to 100 residues. One argument suggesting that this family represents the same enzyme is that no species has a member of both families. Another is that the gene encoding this protein is located near other NAD salvage biosynthesis genes in Nostoc and in at least four different Gram-positive bacteria. NAD and NADP are ubiquitous in life. Most members of this family are Gram-positive bacteria. An additional set of mutually closely related archaeal sequences score between the trusted and noise cutoffs. [Biosynthesis of cofactors, prosthetic groups, and carriers, Pyridine nucleotides]
Pssm-ID: 273666 [Multi-domain] Cd Length: 443 Bit Score: 125.16 E-value: 4.26e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 68 TDEAIALIHTFADQSDQ-----------LEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQV 136
Cdd:TIGR01513 59 DAEDIEYLASLGIFDDAfldylrefrfsGTVRALPEGSLVFPNEPLLQVEGPLIEAQLLETLVLNIINFQTLIATKAARI 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 137 VKAGrtsgKQKPVMFMGDRDDHYTQQAGDGY-AAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVvEAAKAYRD 215
Cdd:TIGR01513 139 VLAA----GGKPLLEFGLRRAQGPDAALKAArAAYIGGADGTSNVLAGRLYGIPVSGTMAHSFVMSFDNEL-AAFRAYAK 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 216 VYPEDeLTVLVD-YN--NDVITDSLKVAHEFKDELK--GVRLDtSGNMVdkyfldnqhlmgsfdprgvnpELVFALRHAL 290
Cdd:TIGR01513 214 LYPKA-TVLLVDtYDtlRSGLPNAIAVAKELGEQGKvvGVRID-SGDLL---------------------YLSKQARKQL 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 291 DEEGFKHVKIIASGGFTEEKIRRFEKQNVPVDIYGVGRSLldikVGFTGDS--------VILDGKPQSKFgrqyNPNPrl 362
Cdd:TIGR01513 271 DAAGLTQVKIVVSNDLDENSIAALKAEGAPIDVYGVGTSL----VTASDAPalggvyklVAYEGRPVMKL----SENP-- 340
|
....*
gi 1397887279 363 EKVSY 367
Cdd:TIGR01513 341 EKSTL 345
|
|
| QRPTase_N |
pfam02749 |
Quinolinate phosphoribosyl transferase, N-terminal domain; Quinolinate phosphoribosyl ... |
49-112 |
1.66e-05 |
|
Quinolinate phosphoribosyl transferase, N-terminal domain; Quinolinate phosphoribosyl transferase (QPRTase) or nicotinate-nucleotide pyrophosphorylase EC:2.4.2.19 is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyzes the reaction of quinolinic acid with 5-phosphoribosyl-1-pyrophosphate (PRPP) in the presence of Mg2+ to give rise to nicotinic acid mononucleotide (NaMN), pyrophosphate and carbon dioxide. The QA substrate is bound between the C-terminal domain of one subunit, and the N-terminal domain of the other. The N-terminal domain has an alpha/beta hammerhead fold.
Pssm-ID: 460674 [Multi-domain] Cd Length: 88 Bit Score: 42.86 E-value: 1.66e-05
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1397887279 49 PNNEVVMQFFQKDHAVLCGTDEAIALIhtfadqsDQLEI---FSLKDGDKISPFETVLTIKGPYQSF 112
Cdd:pfam02749 14 GDKKAKAVIIAKEEGVVAGLEEAERVF-------ELLGLeveWLVKDGDRVEAGDVILEIEGPARAL 73
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PRK07188 |
PRK07188 |
nicotinate phosphoribosyltransferase; Provisional |
15-367 |
0e+00 |
|
nicotinate phosphoribosyltransferase; Provisional
Pssm-ID: 235953 [Multi-domain] Cd Length: 352 Bit Score: 613.49 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 15 RLTNKTFKFDERIETGWFSAVYFLKTKEIAKQKRPNNEVVMQFFQK-DHAVLCGTDEAIALIHTFADQSDQLEIFSLKDG 93
Cdd:PRK07188 1 RLTNKTFKFDERIGEGWYSAVYFLKTREIIEKFNPNNIVTMQFFQRrENAVLCGTDEVIALLKTFAKDPSKLKIRYLKDG 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 94 DKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAGDGYAAFIGG 173
Cdd:PRK07188 81 DIINPFETVLEIEGPYENFGFLEGIIDGILARRTSVATNAYNVVQAAN----EKPVIFMGDRADHYLQQAGDGYAAYIGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 174 STAQATHAMNEWWGRTGIGTMPHALIQLFEGDVVEAAKAYRDVYPEDELTVLVDYNNDVITDSLKVAHEFKDELKGVRLD 253
Cdd:PRK07188 157 ISAFSTHAQNEWWGKSGFGTMPHALIQMFNGDVVEACKAYHKTFPEDELIALVDYNNDVITDSLKVAREFGDKLKGVRVD 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 254 TSGNMVDKYFLDNQHLMGSFDPRGVNPELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNVPVDIYGVGRSLLDI 333
Cdd:PRK07188 237 TSKNMIDKYFIRHPEVLGTFDPRGVNPELIKALRKALDENGGKHVKIIVSSGFDAKKIREFEAQNVPVDIYGVGSSLLKI 316
|
330 340 350
....*....|....*....|....*....|....
gi 1397887279 334 KVGFTGDSVILDGKPQSKFGRQYNPNPRLEKVSY 367
Cdd:PRK07188 317 NIGFTGDAVELNGKKEAKAGRKYRPNPRLERVKL 350
|
|
| NAPRTase_B |
cd01571 |
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup B. Nicotinate ... |
30-354 |
8.62e-138 |
|
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup B. Nicotinate phosphoribosyltransferase catalyses the formation of NAMN and PPi from 5-phosphoribosy -1-pyrophosphate (PRPP) and nicotinic acid, this is the first, and also rate limiting, reaction in the NAD salvage synthesis. This salvage pathway serves to recycle NAD degradation products.
Pssm-ID: 238805 [Multi-domain] Cd Length: 302 Bit Score: 394.33 E-value: 8.62e-138
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 30 GWFSAVYFLKTKEIAKQKRPNNEVVMQFFQKDH--AVLCGTDEAIALIHTFadqsdQLEIFSLKDGDKISPFETVLTIKG 107
Cdd:cd01571 1 GRFTDVYFLRTRKILEKKGPNPTVTMEFTQRSLpwAVLCGLEEVLALLEGL-----PVKVYALPEGTIFNPKEPVLRIEG 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 108 PYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAGDGYAAFIGGSTAQATHAMNEWWG 187
Cdd:cd01571 76 PYQDFGELETAILGILARASSIATNAARVKLAAG----DKPVISFGDRRDHPAIQPMDGRAAYIGGCDGVSTVLGAELLG 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 188 RTGIGTMPHALIQLFEGDVVEAAKAYRDVYPED-ELTVLVDYNNDVITDSLKVAHEFKDELKGVRLDTSGNmvdkyfldn 266
Cdd:cd01571 152 EKPSGTMPHALIQIFGGDQVEAWKAFDETYPEDvPRIALIDTFNDEKEEALKAAKALGDKLDGVRLDTPSS--------- 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 267 qhlmgsfdPRGVNPELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNvpVDIYGVGRSLLDIK-VGFTGDSVILD 345
Cdd:cd01571 223 --------RRGVFRYLIREVRWALDIRGYKHVKIFVSGGLDEEDIKELEDVG--VDAFGVGTAISKAPpVDFTMDIVEVN 292
|
....*....
gi 1397887279 346 GKPQSKFGR 354
Cdd:cd01571 293 GQPIAKRGK 301
|
|
| PRK08662 |
PRK08662 |
nicotinate phosphoribosyltransferase; Reviewed |
24-365 |
4.84e-67 |
|
nicotinate phosphoribosyltransferase; Reviewed
Pssm-ID: 236328 [Multi-domain] Cd Length: 343 Bit Score: 215.12 E-value: 4.84e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 24 DERIETGWFSAVYFLKTKEIAKQKRPNNEVVMQFF-----QKDHAVLCGTDEAIALIhtfadQSDQLEIFSLKDGDKISP 98
Cdd:PRK08662 9 EEEIKSGKTTDIYFERTVEILEHAGKNPKVVAEVTasslpKGEWGVFAGLEEVLELL-----EGKPVDVYALPEGTLFDP 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 99 FETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAgrtsGKQKPVMFMGDRDDHYTQQAGDGYAAFIGGSTAQA 178
Cdd:PRK08662 84 KEPVMRIEGPYLEFGIYETALLGILAHASGIATAAARCKEA----AGDKPVLSFGARHVHPAIAPMMDRAAYIGGCDGVS 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 179 THAMNEWWGRTGIGTMPHALIQLFeGDVVEAAKAYRDVYPEDE-LTVLVDYNNDVITDSLKVAHEFKDELKGVRLDTSGN 257
Cdd:PRK08662 160 GVLGAELLGIEPSGTMPHALILIF-GDQVEAWKAFDEVVPPDVpRIALVDTFKDEREEALRAAEALGDRLDGVRLDTPSS 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 258 MvdkyfldnqhlmgsfdpRGVNPELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQnvpVDIYGVGRSLLDIK-VG 336
Cdd:PRK08662 239 R-----------------RGNFRKIVREVRWTLDIRGYEHVKIFVSGGLDPERIRELRDV---VDGFGVGTYISFAPpVD 298
|
330 340 350
....*....|....*....|....*....|.
gi 1397887279 337 FTGDSVILDGKPQSKFGR--QYNPNPRLEKV 365
Cdd:PRK08662 299 FSMDIVEVEGKPIAKRGKlpGIKQVPRLKEI 329
|
|
| NAPRTase_PncB |
cd01567 |
Nicotinate phosphoribosyltransferase (NAPRTase) family. Nicotinate phosphoribosyltransferase ... |
30-331 |
5.68e-56 |
|
Nicotinate phosphoribosyltransferase (NAPRTase) family. Nicotinate phosphoribosyltransferase catalyses the formation of NAMN and PPi from 5-phosphoribosy -1-pyrophosphate (PRPP) and nicotinic acid, this is the first, and also rate limiting, reaction in the NAD salvage synthesis. This salvage pathway serves to recycle NAD degradation products.
Pssm-ID: 238801 [Multi-domain] Cd Length: 343 Bit Score: 186.32 E-value: 5.68e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 30 GWFSAVYFLKTKEIAKQKRPNNEVVMQFFQK------DHAVLCGTDEAIALIHTF------------------------A 79
Cdd:cd01567 1 LLDTDLYKLTMMQAYLYPYPNTRVVFEFTFRsnpfggDYIVFAGLEEVLKLLENLrfteeeieylkkllifgefflyllF 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 80 DQSDQLEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKA--------GRTSGKQKPVMF 151
Cdd:cd01567 81 LGKLPLEIYALPEGTVVFPKEPLLTIEGPWPEAGLLETPLLAIWNEATSIATKAARKKLAagglletkDNLEELGFKLHD 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 152 MGDRDDHYTQQAG-DGYAAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVVEAAKAYRDVYPE---DELTVLVD 227
Cdd:cd01567 161 FGTRRRHSPEAALsGGRAALIGGFGGTSNVAAAKKLGIPPIGTMAHSWIQAFGALEEAAFEAFARWLPQfggGLGIALID 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 228 YNND--VITDSLKVAHEF--KDELKGVRLDTsgnmvdkyfldnqhlmgsfdprGVNPELVFALRHALDEEGF--KHVKII 301
Cdd:cd01567 241 TYDTdnGFLNALKLAKALgaGGGLLGVRLDS----------------------GDPVELIKKVRKHLDELGIdlNKKKII 298
|
330 340 350
....*....|....*....|....*....|..
gi 1397887279 302 ASGGF-TEEKIRRFEKQN-VPVDIYGVGRSLL 331
Cdd:cd01567 299 ISGDLdTEEAIELLLEQGaSPNDAFGVGTSLT 330
|
|
| PRTase_typeII |
cd00516 |
Phosphoribosyltransferase (PRTase) type II; This family contains two enzymes that play an ... |
35-335 |
2.71e-53 |
|
Phosphoribosyltransferase (PRTase) type II; This family contains two enzymes that play an important role in NAD production by either allowing quinolinic acid (QA) , quinolinate phosphoribosyl transferase (QAPRTase), or nicotinic acid (NA), nicotinate phosphoribosyltransferase (NAPRTase), to be used in the synthesis of NAD. QAPRTase catalyses the reaction of quinolinic acid (QA) with 5-phosphoribosyl-1-pyrophosphate (PRPP) in the presence of Mg2+ to produce nicotinic acid mononucleotide (NAMN), pyrophosphate and carbon dioxide, an important step in the de novo synthesis of NAD. NAPRTase catalyses a similar reaction leading to NAMN and pyrophosphate, using nicotinic acid an PPRP as substrates, used in the NAD salvage pathway.
Pssm-ID: 238286 [Multi-domain] Cd Length: 281 Bit Score: 177.43 E-value: 2.71e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 35 VYFLKTKEIAKqkRPNNEVVMQFFQKDH--AVLCGTDEAIALIHTFaDQSDQLEIFSLKDGDKISPFETVLTIKGPYQSF 112
Cdd:cd00516 2 LYKLTMIQAYP--PPDTRATAEFTAREDpyGVLAGLEEALELLELL-RFPGPLVILAVPEGTVVEPGEPLLTIEGPAREL 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 113 GFLEGIIDGILARRTSVATNVYQVVKAGRtsGKQKPVMFMGDRDDHYTQQAGDGYAAFIGGSTAQATHAMNEWWGRTGIG 192
Cdd:cd00516 79 LLLERVLLNLLQRLSGIATATARYVEAAK--GANTKVHDFGTRKTTPGLRLLEKYAVLIGGGDGHRNGLSDAILIKDNHG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 193 TMPHALIQLFegDVVEAAKAYRDVYPEDeLTVLVDYNNDVITDSLKVAHEFKdeLKGVRLDtsgnmvdkyfldnqhlmgS 272
Cdd:cd00516 157 TMAHSIIQAF--GELAAVKALRRWLPEL-FIALIDVEVDTLEEALEAAKAGG--ADGIRLD------------------S 213
|
250 260 270 280 290 300
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1397887279 273 FDPRGVNP-ELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNvpVDIYGVGRSL-----LDIKV 335
Cdd:cd00516 214 GSPEELDPaVLILKARAHLDGKGLPRVKIEASGGLDEENIRAYAETG--VDVFGVGTLLhsappLDIVL 280
|
|
| PncB |
COG1488 |
Nicotinic acid phosphoribosyltransferase [Coenzyme transport and metabolism]; Nicotinic acid ... |
85-331 |
5.53e-33 |
|
Nicotinic acid phosphoribosyltransferase [Coenzyme transport and metabolism]; Nicotinic acid phosphoribosyltransferase is part of the Pathway/BioSystem: NAD biosynthesis
Pssm-ID: 441097 [Multi-domain] Cd Length: 445 Bit Score: 127.57 E-value: 5.53e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 85 LEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRtsgkQKPVMFMGDRDDHYTQQAG 164
Cdd:COG1488 91 GDIRAVPEGTVVFPGEPLLTVEGTLPEAQLLETLLLNILNYPTLIATKAARIVQAAG----GRPLHDFGLRRAHGLEAAV 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 165 DG-YAAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVvEAAKAYRDVYPEDeLTVLVD-YN-NDVITDSLKVAH 241
Cdd:COG1488 167 AAaRAAYLAGFAGTSNVLAGRYYGIPSVGTMAHSFVQAHDDEL-EAFRAFAEAYPDG-TTLLVDtYDtLSGVPNAIELAK 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 242 EF--KDELKGVRLDtSGNMVDkyfldnqhlmgsfdprgvnpeLVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNV 319
Cdd:COG1488 245 ELlaRGGLLGIRLD-SGDLAY---------------------LSKQARELLDEAGLPDVRIIASDDLDEYRIAALLEQGA 302
|
250
....*....|..
gi 1397887279 320 PVDIYGVGRSLL 331
Cdd:COG1488 303 PIDVFGVGTRLV 314
|
|
| NAPRTase_put |
TIGR01513 |
putative nicotinate phosphoribosyltransferase; A deep split separates two related families of ... |
68-367 |
4.26e-32 |
|
putative nicotinate phosphoribosyltransferase; A deep split separates two related families of proteins, one of which includes experimentally characterized examples of nicotinate phosphoribosyltransferase, an the first enzyme of NAD salvage biosynthesis. This model represents the other family. Members have a different (longer) spacing of several key motifs and have an additional C-terminal domain of up to 100 residues. One argument suggesting that this family represents the same enzyme is that no species has a member of both families. Another is that the gene encoding this protein is located near other NAD salvage biosynthesis genes in Nostoc and in at least four different Gram-positive bacteria. NAD and NADP are ubiquitous in life. Most members of this family are Gram-positive bacteria. An additional set of mutually closely related archaeal sequences score between the trusted and noise cutoffs. [Biosynthesis of cofactors, prosthetic groups, and carriers, Pyridine nucleotides]
Pssm-ID: 273666 [Multi-domain] Cd Length: 443 Bit Score: 125.16 E-value: 4.26e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 68 TDEAIALIHTFADQSDQ-----------LEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQV 136
Cdd:TIGR01513 59 DAEDIEYLASLGIFDDAfldylrefrfsGTVRALPEGSLVFPNEPLLQVEGPLIEAQLLETLVLNIINFQTLIATKAARI 138
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 137 VKAGrtsgKQKPVMFMGDRDDHYTQQAGDGY-AAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVvEAAKAYRD 215
Cdd:TIGR01513 139 VLAA----GGKPLLEFGLRRAQGPDAALKAArAAYIGGADGTSNVLAGRLYGIPVSGTMAHSFVMSFDNEL-AAFRAYAK 213
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 216 VYPEDeLTVLVD-YN--NDVITDSLKVAHEFKDELK--GVRLDtSGNMVdkyfldnqhlmgsfdprgvnpELVFALRHAL 290
Cdd:TIGR01513 214 LYPKA-TVLLVDtYDtlRSGLPNAIAVAKELGEQGKvvGVRID-SGDLL---------------------YLSKQARKQL 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 291 DEEGFKHVKIIASGGFTEEKIRRFEKQNVPVDIYGVGRSLldikVGFTGDS--------VILDGKPQSKFgrqyNPNPrl 362
Cdd:TIGR01513 271 DAAGLTQVKIVVSNDLDENSIAALKAEGAPIDVYGVGTSL----VTASDAPalggvyklVAYEGRPVMKL----SENP-- 340
|
....*
gi 1397887279 363 EKVSY 367
Cdd:TIGR01513 341 EKSTL 345
|
|
| NAPRTase_A |
cd01570 |
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup A. Nicotinate ... |
84-330 |
3.40e-29 |
|
Nicotinate phosphoribosyltransferase (NAPRTase), subgroup A. Nicotinate phosphoribosyltransferase catalyses the formation of NAMN and PPi from 5-phosphoribosy -1-pyrophosphate (PRPP) and nicotinic acid, this is the first, and also rate limiting, reaction in the NAD salvage synthesis. This salvage pathway serves to recycle NAD degradation products. This subgroup is present in bacteria and eukaryota (except funghi).
Pssm-ID: 238804 [Multi-domain] Cd Length: 327 Bit Score: 114.98 E-value: 3.40e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 84 QLEIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGrtsgKQKPVMFMGDRDDHYTQQA 163
Cdd:cd01570 86 TGTIYAIPEGEVVFPNEPLLTVEGPLIEAQLLETLLLNLINFQTLIATKAARVRLAA----GGRPLLEFGLRRAQGPDAA 161
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 164 GDGY-AAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFEGDVvEAAKAYRDVYPeDELTVLVD-YN--NDVITDSLKV 239
Cdd:cd01570 162 LSAArAAYIGGFDGTSNVLAGKLYGIPVSGTMAHSFVQAFDDEL-AAFRAFAEAYP-DNFTLLVDtYDtlRSGLPNAIAV 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 240 AHEFKD---ELKGVRLDtSGNMVdkyfldnqhlmgsfdprgvnpELVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEK 316
Cdd:cd01570 240 AKELGAlgyRLVGVRID-SGDLA---------------------YLSKEARKMLDEAGLTKVKIVASNDLDEYTIAALNA 297
|
250
....*....|....
gi 1397887279 317 QNVPVDIYGVGRSL 330
Cdd:cd01570 298 QGAPIDAFGVGTRL 311
|
|
| PRK09243 |
PRK09243 |
nicotinate phosphoribosyltransferase; Validated |
87-330 |
5.93e-28 |
|
nicotinate phosphoribosyltransferase; Validated
Pssm-ID: 236426 [Multi-domain] Cd Length: 464 Bit Score: 113.71 E-value: 5.93e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 87 IFSLKDGDKISPFETVLTIKGPyqsFG---FLEGIIDGILARRTSVATnvyqvvKAGR--TSGKQKPVMFMGDRDDHytq 161
Cdd:PRK09243 98 VRAVPEGELVFPNEPLLRVEGP---LAeaqLLETLLLNIINFQTLIAT------KAARivSAAGGRPLLEFGSRRAQ--- 165
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 162 qagdGY--------AAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFeGDVVEAAKAYRDVYPeDELTVLVD-YnnDV 232
Cdd:PRK09243 166 ----GPdaavwaarAAYIGGFDATSNVLAGKRYGIPVSGTMAHSFVQAF-DDEYEAFRAYAETYP-DNTVLLVDtY--DT 237
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 233 ITDSL----KVAHEFKD--ELKGVRLDtSGNMVdkyfldnqhlmgsfdprgvnpELVFALRHALDEEGFKHVKIIASGGF 306
Cdd:PRK09243 238 LKSGVpnaiKVAKELGDgiELGGVRID-SGDLA---------------------YLSKKVRKMLDEAGFTDTKIVASNDL 295
|
250 260
....*....|....*....|....
gi 1397887279 307 TEEKIRRFEKQNVPVDIYGVGRSL 330
Cdd:PRK09243 296 DEYTIASLKLQGAPIDGFGVGTKL 319
|
|
| PRK12484 |
PRK12484 |
nicotinate phosphoribosyltransferase; Provisional |
86-346 |
2.89e-21 |
|
nicotinate phosphoribosyltransferase; Provisional
Pssm-ID: 237112 [Multi-domain] Cd Length: 443 Bit Score: 94.43 E-value: 2.89e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 86 EIFSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVYQVVKAGRTsgkqKPVMFMGDRDDHYTQQAGD 165
Cdd:PRK12484 91 DVRAVPEGTVVFPNEPLLEVTAPLIEAQLVETFLLNQINHQSLIASKAARCVLAAAG----RPVVDFGARRAHGTDAACY 166
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 166 G-YAAFIGGSTAQATHAMNEWWGRTGIGTMPHALIQLFeGDVVEAAKAYRDVYPeDELTVLVDYNNDV--ITDSLKVAHE 242
Cdd:PRK12484 167 VaRASYLAGAAGTSNVLAARQYGIPASGTMAHSFVEAF-PDEVAAFRAFARLYP-DATTLLVDTYDTLrgVRNAIEVAKE 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 243 FKD--ELKGVRLDtSGNMVDkyfldnqhlmgsfdprgvnpeLVFALRHALDEEGFKHVKIIASGGFTEEKIRRFEKQNVP 320
Cdd:PRK12484 245 LGNrfDPRGVRLD-SGDLAE---------------------LSKATRAILDAAGLEQVKIVASGGLDEYRIAALLAAGAP 302
|
250 260
....*....|....*....|....*.
gi 1397887279 321 VDIYGVGRslldiKVGFTGDSVILDG 346
Cdd:PRK12484 303 IDGFGVGT-----RLGVAADAPVLDS 323
|
|
| QRPTase_N |
pfam02749 |
Quinolinate phosphoribosyl transferase, N-terminal domain; Quinolinate phosphoribosyl ... |
49-112 |
1.66e-05 |
|
Quinolinate phosphoribosyl transferase, N-terminal domain; Quinolinate phosphoribosyl transferase (QPRTase) or nicotinate-nucleotide pyrophosphorylase EC:2.4.2.19 is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyzes the reaction of quinolinic acid with 5-phosphoribosyl-1-pyrophosphate (PRPP) in the presence of Mg2+ to give rise to nicotinic acid mononucleotide (NaMN), pyrophosphate and carbon dioxide. The QA substrate is bound between the C-terminal domain of one subunit, and the N-terminal domain of the other. The N-terminal domain has an alpha/beta hammerhead fold.
Pssm-ID: 460674 [Multi-domain] Cd Length: 88 Bit Score: 42.86 E-value: 1.66e-05
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1397887279 49 PNNEVVMQFFQKDHAVLCGTDEAIALIhtfadqsDQLEI---FSLKDGDKISPFETVLTIKGPYQSF 112
Cdd:pfam02749 14 GDKKAKAVIIAKEEGVVAGLEEAERVF-------ELLGLeveWLVKDGDRVEAGDVILEIEGPARAL 73
|
|
| QPRTase_NadC |
cd01568 |
Quinolinate phosphoribosyl transferase (QAPRTase or QPRTase), also called ... |
49-141 |
7.62e-05 |
|
Quinolinate phosphoribosyl transferase (QAPRTase or QPRTase), also called nicotinate-nucleotide pyrophosphorylase, is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyses the reaction of quinolinic acid (QA) with 5-phosphoribosyl-1-pyrophosphate (PRPP) in the presence of Mg2+ to produce nicotinic acid mononucleotide (NAMN), pyrophosphate and carbon dioxide. QPRTase functions as a homodimer with two active sites, each formed by the C-terminal region of one subunit and the N-terminal region of the other.
Pssm-ID: 238802 [Multi-domain] Cd Length: 269 Bit Score: 44.00 E-value: 7.62e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 49 PNNEVVMQFFQKDHAVLCGTDEAIALIhtfaDQSDQLEI-FSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRT 127
Cdd:cd01568 25 GDAPATATLIAKEEGVLAGLEVAEEVF----ELLDGIEVeWLVKDGDRVEAGQVLLEVEGPARSLLTAERVALNLLQRLS 100
|
90
....*....|....
gi 1397887279 128 SVATNVYQVVKAGR 141
Cdd:cd01568 101 GIATATRRYVEAAR 114
|
|
| QPRTase |
cd01572 |
Quinolinate phosphoribosyl transferase (QAPRTase or QPRTase), also called ... |
56-139 |
1.80e-04 |
|
Quinolinate phosphoribosyl transferase (QAPRTase or QPRTase), also called nicotinate-nucleotide pyrophosphorylase, is involved in the de novo synthesis of NAD in both prokaryotes and eukaryotes. It catalyses the reaction of quinolinic acid (QA) with 5-phosphoribosyl-1-pyrophosphate (PRPP) in the presence of Mg2+ to produce nicotinic acid mononucleotide (NAMN), pyrophosphate and carbon dioxide. QPRTase functions as a homodimer with two active sites, each formed by the C-terminal region of one subunit and the N-terminal region of the other.
Pssm-ID: 238806 [Multi-domain] Cd Length: 268 Bit Score: 42.85 E-value: 1.80e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1397887279 56 QFFQKDHAVLCGTDEAIALihtFADQSDQLEI-FSLKDGDKISPFETVLTIKGPYQSFGFLEGIIDGILARRTSVATNVY 134
Cdd:cd01572 32 RLIAKEEGVLAGLPVAEEV---FELLDPGIEVeWLVKDGDRVEPGQVLATVEGPARSLLTAERTALNFLQRLSGIATLTR 108
|
....*
gi 1397887279 135 QVVKA 139
Cdd:cd01572 109 RYVEA 113
|
|
| |
