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Conserved domains on  [gi|1483050807|gb|RJQ17375|]
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hypothetical protein C4573_03115 [Candidatus Woesearchaeota archaeon]

Protein Classification

nucleotidyltransferase family protein( domain architecture ID 10004143)

nucleotidyltransferase family protein may be involved in the transfer of a phosphorous-containing group, such as a nucleoside monophosphate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
1-90 3.94e-18

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


:

Pssm-ID: 441275  Cd Length: 96  Bit Score: 71.87  E-value: 3.94e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807  1 MNRKKLLQIVVMNL----KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENS 74
Cdd:COG1669    1 MNREEILEILREVIeelaERYGVSRLGLFGSVARGEAREdsDIDLLVEFDEPTSLFDLFELEEELEELLGRKVDLVTLDG 80

                         90
                 ....*....|....*.
gi 1483050807 75 ISPFLKESIGKDIEVL 90
Cdd:COG1669   81 LSPRLRERILKEGVLL 96
 
Name Accession Description Interval E-value
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
1-90 3.94e-18

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


Pssm-ID: 441275  Cd Length: 96  Bit Score: 71.87  E-value: 3.94e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807  1 MNRKKLLQIVVMNL----KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENS 74
Cdd:COG1669    1 MNREEILEILREVIeelaERYGVSRLGLFGSVARGEAREdsDIDLLVEFDEPTSLFDLFELEEELEELLGRKVDLVTLDG 80

                         90
                 ....*....|....*.
gi 1483050807 75 ISPFLKESIGKDIEVL 90
Cdd:COG1669   81 LSPRLRERILKEGVLL 96
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 15-91 1.71e-08

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 47.41  E-value: 1.71e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1483050807 15 KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENSISPFLKESIGKDIEVLY 91
Cdd:cd05403   14 LLGGVEKVYLFGSYARGDARPdsDIDLLVIFDDPLDPLELARLLEELELLLGRPVDLVVLNALELELLLRILIEGEGIY 92
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 15-91 1.64e-06

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


Pssm-ID: 465859  Cd Length: 93  Bit Score: 42.13  E-value: 1.64e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807 15 KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSE-STGVKIDMLTENSISPFLKESIGKDIEVLY 91
Cdd:pfam18765  9 KNPEIEAAYLFGSRAKGDAREdsDIDLAVLYDEKLDFEELLELASELEDiLLLRKVDLVDLNKAPPVLAHQILKEGKLLY 88
 
Name Accession Description Interval E-value
MJ0435 COG1669
Predicted nucleotidyltransferase MJ0435 [General function prediction only];
1-90 3.94e-18

Predicted nucleotidyltransferase MJ0435 [General function prediction only];


Pssm-ID: 441275  Cd Length: 96  Bit Score: 71.87  E-value: 3.94e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807  1 MNRKKLLQIVVMNL----KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENS 74
Cdd:COG1669    1 MNREEILEILREVIeelaERYGVSRLGLFGSVARGEAREdsDIDLLVEFDEPTSLFDLFELEEELEELLGRKVDLVTLDG 80

                         90
                 ....*....|....*.
gi 1483050807 75 ISPFLKESIGKDIEVL 90
Cdd:COG1669   81 LSPRLRERILKEGVLL 96
NT_KNTase_like cd05403
Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, ...
 15-91 1.71e-08

Nucleotidyltransferase (NT) domain of Staphylococcus aureus kanamycin nucleotidyltransferase, and similar proteins; S. aureus KNTase is a plasmid encoded enzyme which confers resistance to a wide range of aminoglycoside antibiotics which have a 4'- or 4''-hydroxyl group in the equatorial position, such as kanamycin A. This enzyme transfers a nucleoside monophosphate group from a nucleotide (ATP,GTP, or UTP) to the 4'-hydroxyl group of kanamycin A. This enzyme is a homodimer, having two NT active sites. The nucleotide and antibiotic binding sites of each active site include residues from each monomer. Included in this subgroup is Escherichia coli AadA5 which confers resistance to the antibiotic spectinomycin and is a putative aminoglycoside-3'-adenylyltransferase. It is part of the aadA5 cassette of a class 1 integron. This subgroup also includes Haemophilus influenzae HI0073 which forms a 2:2 heterotetramer with an unrelated protein HI0074. Structurally HI0074 is related to the substrate-binding domain of S. aureus KNTase. The genes encoding HI0073 and HI0074 form an operon. Little is known about the substrate specificity or function of two-component NTs. The characterized members of this subgroup may not be representive of the function of this subgroup. This subgroup belongs to the Pol beta-like NT superfamily. In the majority of enzymes in this superfamily, two carboxylates, Dx[D/E], together with a third more distal carboxylate, co-ordinate two divalent metal cations involved in a two-metal ion mechanism of nucleotide addition. These carboxylate residues are conserved in this subgroup.


Pssm-ID: 143393  Cd Length: 93  Bit Score: 47.41  E-value: 1.71e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1483050807 15 KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENSISPFLKESIGKDIEVLY 91
Cdd:cd05403   14 LLGGVEKVYLFGSYARGDARPdsDIDLLVIFDDPLDPLELARLLEELELLLGRPVDLVVLNALELELLLRILIEGEGIY 92
Polbeta pfam18765
Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in ...
 15-91 1.64e-06

Polymerase beta, Nucleotidyltransferase; A member of the nucleotidyltransferase fold found in polymorphic toxins (NTox45) and polyvalent proteins.


Pssm-ID: 465859  Cd Length: 93  Bit Score: 42.13  E-value: 1.64e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807 15 KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSE-STGVKIDMLTENSISPFLKESIGKDIEVLY 91
Cdd:pfam18765  9 KNPEIEAAYLFGSRAKGDAREdsDIDLAVLYDEKLDFEELLELASELEDiLLLRKVDLVDLNKAPPVLAHQILKEGKLLY 88
NTP_transf_2 pfam01909
Nucleotidyltransferase domain; Members of this family belong to a large family of ...
 4-83 1.55e-05

Nucleotidyltransferase domain; Members of this family belong to a large family of nucleotidyltransferases. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.


Pssm-ID: 396474  Cd Length: 91  Bit Score: 39.71  E-value: 1.55e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1483050807  4 KKLLQIVVMNLKKegaKKISLFGSYARKEKHK--DIDLIVAFK---QQKSLLELIRIERELSESTGVKIDMLTENSI-SP 77
Cdd:pfam01909  2 RKLREILKELFPV---AEVVLFGSYARGTALPgsDIDLLVVFPepvEEERLLKLAKIIKELEELLGLEVDLVTREKIeFP 78


                 ....*.
gi 1483050807 78 FLKESI 83
Cdd:pfam01909 79 LVKIDI 84
MJ0604 COG1708
Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];
15-87 3.49e-05

Predicted nucleotidyltransferase, MJ0604 family [General function prediction only];


Pssm-ID: 441314  Cd Length: 95  Bit Score: 38.86  E-value: 3.49e-05
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1483050807 15 KKEGAKKISLFGSYARKEKHK--DIDLIVAFKQQKSLLELIRIERELSESTGVKIDMLTENSiSPFLKESIGKDI 87
Cdd:COG1708   18 RGPEVAAVYLFGSYARGDARPdsDIDLLVVVDDPPLPDERLELLADLLRELGLPVDLVVLTP-AELELRLFLREI 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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