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Conserved domains on  [gi|1490467405|gb|RLA98991|]
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HIT family hydrolase [Deltaproteobacteria bacterium]

Protein Classification

HIT family protein( domain architecture ID 10101080)

HIT (Histidine triad) family protein, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), is part of a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides; similar to Homo sapiens Bis(5'-adenosyl)-triphosphatase (also called fragile histidine triad protein) that cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP

CATH:  3.30.428.10
Gene Ontology:  GO:0003824
PubMed:  1472710
SCOP:  4000950

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 21-148 6.21e-54

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


:

Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 166.70  E-value: 6.21e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  21 CIFCTPLQQRDDRrNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGF 100
Cdd:cd01275     1 CVFCDIPIKPDED-NLVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGF 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1490467405 101 NIGANVGKvAGAGVEGHVHFHIVPRWEGDTNYMPVLASTRVIPEHLQQ 148
Cdd:cd01275    80 NIGINDGK-AGGGIVPHVHIHIVPRWNGDTNFMPVIIYTKVLPEDLED 126
 
Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 21-148 6.21e-54

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 166.70  E-value: 6.21e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  21 CIFCTPLQQRDDRrNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGF 100
Cdd:cd01275     1 CVFCDIPIKPDED-NLVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGF 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1490467405 101 NIGANVGKvAGAGVEGHVHFHIVPRWEGDTNYMPVLASTRVIPEHLQQ 148
Cdd:cd01275    80 NIGINDGK-AGGGIVPHVHIHIVPRWNGDTNFMPVIIYTKVLPEDLED 126
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 19-155 2.35e-46

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 147.79  E-value: 2.35e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  19 DSCIFCTPLqqRDDRRNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPH 98
Cdd:COG0537     1 MDCIFCKII--AGEIPALIVYEDEHVLAFLDINPYAPGHTLVIPKRHVASLFDLTPEELAELMRLAQKVAKALRKALGPD 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1490467405  99 GFNIGANVGKVAGAGVEgHVHFHIVPRWEGDTNYMPVLAsTRVIPEHLQQTYDRLRD 155
Cdd:COG0537    79 GFNLGINNGEAAGQTVP-HLHVHVIPRYEGDDNFMPVIG-TKVDPEELEETARKLRA 133
HIT pfam01230
HIT domain;
42-128 5.85e-13

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 61.17  E-value: 5.85e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  42 KESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGFNIGANVGKVAGAGVEgHVHFH 121
Cdd:pfam01230  13 DLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADGYRIVINNGAHAGQSVP-HLHIH 91


                  ....*..
gi 1490467405 122 IVPRWEG 128
Cdd:pfam01230  92 VIPRRKH 98
 
Name Accession Description Interval E-value
FHIT cd01275
FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH ...
 21-148 6.21e-54

FHIT (fragile histidine family): FHIT proteins, related to the HIT family carry a motif HxHxH/Qxx (x, is a hydrophobic amino acid), On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified into three branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Fhit plays a very important role in the development of tumours. Infact, Fhit deletions are among the earliest and most frequent genetic alterations in the development of tumours.


Pssm-ID: 238606 [Multi-domain]  Cd Length: 126  Bit Score: 166.70  E-value: 6.21e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  21 CIFCTPLQQRDDRrNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGF 100
Cdd:cd01275     1 CVFCDIPIKPDED-NLVFYRTKHSFAVVNLYPYNPGHVLVVPYRHVPRLEDLTPEEIADLFKLVQLAMKALKVVYKPDGF 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1490467405 101 NIGANVGKvAGAGVEGHVHFHIVPRWEGDTNYMPVLASTRVIPEHLQQ 148
Cdd:cd01275    80 NIGINDGK-AGGGIVPHVHIHIVPRWNGDTNFMPVIIYTKVLPEDLED 126
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 19-155 2.35e-46

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 147.79  E-value: 2.35e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  19 DSCIFCTPLqqRDDRRNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPH 98
Cdd:COG0537     1 MDCIFCKII--AGEIPALIVYEDEHVLAFLDINPYAPGHTLVIPKRHVASLFDLTPEELAELMRLAQKVAKALRKALGPD 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1490467405  99 GFNIGANVGKVAGAGVEgHVHFHIVPRWEGDTNYMPVLAsTRVIPEHLQQTYDRLRD 155
Cdd:COG0537    79 GFNLGINNGEAAGQTVP-HLHVHVIPRYEGDDNFMPVIG-TKVDPEELEETARKLRA 133
HINT_subgroup cd01277
HINT (histidine triad nucleotide-binding protein) subgroup: Members of this CD belong to the ...
 20-125 7.10e-19

HINT (histidine triad nucleotide-binding protein) subgroup: Members of this CD belong to the superfamily of histidine triad hydrolases that act on alpha-phosphate of ribonucleotides. This subgroup includes members from all three forms of cellular life. Although the biochemical function has not been characterised for many of the members of this subgroup, the proteins from Yeast have been shown to be involved in secretion, peroxisome formation and gene expression.


Pssm-ID: 238608 [Multi-domain]  Cd Length: 103  Bit Score: 76.49  E-value: 7.10e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  20 SCIFCTPLqqRDDRRNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHG 99
Cdd:cd01277     1 DCIFCKII--AGEIPSYKVYEDDHVLAFLDINPASKGHTLVIPKKHYENLLDLDPEELAELILAAKKVARALKKALKADG 78

                          90       100
                  ....*....|....*....|....*.
gi 1490467405 100 FNIGANVGKVAGAGVeGHVHFHIVPR 125
Cdd:cd01277    79 LNILQNNGRAAGQVV-FHVHVHVIPR 103
HIT pfam01230
HIT domain;
42-128 5.85e-13

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 61.17  E-value: 5.85e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  42 KESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGFNIGANVGKVAGAGVEgHVHFH 121
Cdd:pfam01230  13 DLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADGYRIVINNGAHAGQSVP-HLHIH 91


                  ....*..
gi 1490467405 122 IVPRWEG 128
Cdd:pfam01230  92 VIPRRKH 98
HIT_like cd00468
HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH ...
 39-124 6.25e-10

HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), are a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides. On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified in the literacture into three major branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Further sequence analysis reveals several new closely related, yet uncharacterized subgroups.


Pssm-ID: 238263 [Multi-domain]  Cd Length: 86  Bit Score: 52.86  E-value: 6.25e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  39 HRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKGAMSPHGFNIGANVGKVAGAGVEgHV 118
Cdd:cd00468     2 PDDEHSFAFVNLKPAAPGHVLVCPKRHVETLPDLDEALLADLVITAQRVAAELEKHGNVPSLTVFVNDGAAAGQSVP-HV 80


                  ....*.
gi 1490467405 119 HFHIVP 124
Cdd:cd00468    81 HLHVLP 86
PKCI_related cd01276
Protein Kinase C Interacting protein related (PKCI): PKCI and related proteins belong to the ...
 57-122 3.04e-04

Protein Kinase C Interacting protein related (PKCI): PKCI and related proteins belong to the ubiquitous HIT family of hydrolases that act on alpha-phosphates of ribonucleotides. The members of this subgroup have a conserved HxHxHxx motif (x is a hydrophobic residue) that is a signature for this family. No enzymatic activity has been reported however, for PKCI and its related members.


Pssm-ID: 238607 [Multi-domain]  Cd Length: 104  Bit Score: 38.31  E-value: 3.04e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1490467405  57 HLMVAPYRHIGLLEEL---SAEEVKDLMINIQLSIRVLKGAMSphGFNIGANVGKVAGAGVeGHVHFHI 122
Cdd:cd01276    36 HILVIPKKHIASLSDAteeDEELLGHLLSAAAKVAKDLGIAED--GYRLVINCGKDGGQEV-FHLHLHL 101
GalT COG1085
Galactose-1-phosphate uridylyltransferase [Carbohydrate transport and metabolism];
20-125 4.65e-04

Galactose-1-phosphate uridylyltransferase [Carbohydrate transport and metabolism];


Pssm-ID: 440702 [Multi-domain]  Cd Length: 336  Bit Score: 39.43  E-value: 4.65e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1490467405  20 SCIFCTPLQQ--RDDRRnlILHRGkESFIIL----NRYPYtngHLMVAPYRHIGLLEELSAEEVKDLMINIQLSIRVLKG 93
Cdd:COG1085   191 RCLLCDILAQelAAGER--VVAEN-EHFVAFvpfaARWPF---ETWILPKRHVSDFEELTDEERDDLARILKRVLRRLDN 264

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1490467405  94 AMSPHGFNIGANVGKVAGAGVEG-HVHFHIVPR 125
Cdd:COG1085   265 LLGDFPYNMGLHQAPVDGEERDHyHWHLEIYPR 297
aprataxin_related cd01278
aprataxin related: Aprataxin, a HINT family hydrolase is mutated in ataxia oculomotor apraxia ...
 20-77 6.87e-04

aprataxin related: Aprataxin, a HINT family hydrolase is mutated in ataxia oculomotor apraxia syndrome. All the members of this subgroup have the conserved HxHxHxx (where x is a hydrophobic residue) signature motif. Members of this subgroup are predominantly eukaryotic in origin.


Pssm-ID: 238609 [Multi-domain]  Cd Length: 104  Bit Score: 37.37  E-value: 6.87e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1490467405  20 SCIFCTPLQQRDDRRNLILHRGKESFIILNRYPYTNGHLMVAPYRHIGLLEELSAEEV 77
Cdd:cd01278     1 LCHFCDIAKRRDPDPEDQVYEDDRVVVFKDIYPKARHHYLVIPKEHIASLKALTKEDV 58
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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