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Conserved domains on  [gi|1079632717|emb|SCW28764|]
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BirA family transcriptional regulator, biotin operon repressor / biotin-[acetyl-CoA-carboxylase] ligase [Ruminococcaceae bacterium YRB3002]

Protein Classification

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor( domain architecture ID 11447346)

bifunctional biotin--[acetyl-CoA-carboxylase]ligase/biotin operon repressor acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor; it biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 80-325 1.98e-85

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


:

Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 257.02  E-value: 1.98e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:COG0340     1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRPEDfPPELRETASSLGIYSGnYEL 239
Cdd:COG0340    81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEAS--GEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETG-KEV 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 240 SRAALAAAMIRELDRMNDSWPSDK-SSYFNTYRDNCVTVGKNVDVTNyaTGAVRHGYAVDVNEDFSLKVRFENGDTEDVR 318
Cdd:COG0340   157 DREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVET--GGETLEGIAVGIDEDGALLLETADGEIRAVA 234


                  ....*..
gi 1079632717 319 SGEVSVK 325
Cdd:COG0340   235 AGEVSLR 241
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 6-55 1.90e-09

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


:

Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 52.82  E-value: 1.90e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1079632717   6 VLAILIKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGY 55
Cdd:pfam08279   3 ILQLLLEARGPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 80-325 1.98e-85

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 257.02  E-value: 1.98e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:COG0340     1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRPEDfPPELRETASSLGIYSGnYEL 239
Cdd:COG0340    81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEAS--GEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETG-KEV 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 240 SRAALAAAMIRELDRMNDSWPSDK-SSYFNTYRDNCVTVGKNVDVTNyaTGAVRHGYAVDVNEDFSLKVRFENGDTEDVR 318
Cdd:COG0340   157 DREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVET--GGETLEGIAVGIDEDGALLLETADGEIRAVA 234


                  ....*..
gi 1079632717 319 SGEVSVK 325
Cdd:COG0340   235 AGEVSLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 80-254 1.15e-59

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 189.01  E-value: 1.15e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:cd16442     1 KLIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPAEAPLLTLLAAVAVA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSVEGEigMISNIIVGIGVNVRQRPEDFPPELRETASSLGIysgnyEL 239
Cdd:cd16442    81 EALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGE--GVAAVVIGIGINVNNTPPPEPLPDTSLATSLGK-----EV 153

                         170
                  ....*....|....*
gi 1079632717 240 SRAALAAAMIRELDR 254
Cdd:cd16442   154 DRNELLEELLAALEN 168
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
1-324 7.83e-58

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 189.23  E-value: 7.83e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717   1 MTKDEVLAILiKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGYRLISCPDRLYAGEIYAYLNKERCSn 80
Cdd:PRK11886    4 TVMLQLLSLL-ADGDFHSGEQLGEELGISRAAIWKHIQTLEEWGLDIFSVKGKGYRLAEPLDLLDPERISSQLPPGRVT- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  81 iiCLPSVDSTNNILKsMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVHR 160
Cdd:PRK11886   82 --VLPVIDSTNQYLL-DRIAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRLNQGPAQAMGLSLVVGIAIAE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 161 AILSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRpeDFPPELRETA----SSLGIysgn 236
Cdd:PRK11886  159 ALRRLGAIDVGLKWPNDIYLNDRKLAGILVELS--GETGDAAHVVIGIGINVAMP--DFPEELIDQPwsdlQEAGP---- 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 237 yELSRAALAAAMIRELDR-MNDSWPSDKSSYFNTYRDNCVTVGKNVDVTnyaTGAVR-HGYAVDVNEDFSLKVRfENGDT 314
Cdd:PRK11886  231 -TIDRNQLAAELIKQLRAaLELFEQEGLAPFLERWKKLDLFLGREVKLI---IGDKEiSGIARGIDEQGALLLE-DDGVE 305

                         330
                  ....*....|
gi 1079632717 315 EDVRSGEVSV 324
Cdd:PRK11886  306 KPFNGGEISL 315
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 81-323 1.16e-42

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 147.16  E-value: 1.16e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  81 IICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADISEITCWTAVAVHR 160
Cdd:TIGR00121   2 VIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILRPDLPKSPAPGLTLVAGIAIAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 161 AiLSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRpeDFPPELRETASSLGIYSGnYELS 240
Cdd:TIGR00121  81 V-LKELGDQVQVKWPNDILLKDKKLGGILTELT--GKENRADYVVIGIGINVQNR--KPAESLREQAISLSEEAG-IDLD 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 241 RAALAAAMIRELD-RMNDSWPSDKSSYFNTYRDNCVTVGKNVDVTNYATGAVrhGYAVDVNEDFSLKVRfENGDTEDVRS 319
Cdd:TIGR00121 155 RGELIEGFLRNFEeNLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIE--GIARGIDKDGALLLE-DGGGIKKIIS 231


                  ....
gi 1079632717 320 GEVS 323
Cdd:TIGR00121 232 GEIS 235
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 85-211 6.35e-18

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 78.64  E-value: 6.35e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  85 PSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADISEI---TCWTAVAVHRA 161
Cdd:pfam03099   3 ERIKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKG-CLTYSLLLSKEHPNVDPSVLefyVLELVLAVLEA 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1079632717 162 ILSVF----DIRTDIKWVNDLFLNGRKITGILTEmSVEGEIgmISNIIVGIGVN 211
Cdd:pfam03099  82 LGLYKpgisGIPCFVKWPNDLYVNGRKLAGILQR-STRGGT--LHHGVIGLGVN 132
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 6-55 1.90e-09

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 52.82  E-value: 1.90e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1079632717   6 VLAILIKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGY 55
Cdd:pfam08279   3 ILQLLLEARGPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
MntR COG1321
Mn-dependent transcriptional regulator MntR, DtxR family [Transcription];
5-57 4.75e-04

Mn-dependent transcriptional regulator MntR, DtxR family [Transcription];


Pssm-ID: 440932 [Multi-domain]  Cd Length: 135  Bit Score: 39.80  E-value: 4.75e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1079632717   5 EVLAILIKKADYVSGESISSEIGVSRAAVNGAVKSLKADGcLIDSVTNKGYRL 57
Cdd:COG1321    13 KAIYELSEEGGPVRTSDIAERLGVSPPSVTEMLKKLEEKG-LVEYEPYGGITL 64
 
Name Accession Description Interval E-value
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 80-325 1.98e-85

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 257.02  E-value: 1.98e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:COG0340     1 RIEVFDEVDSTNDEAKELAREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLRPDLPPARLPLLSLAAGLAVA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRPEDfPPELRETASSLGIYSGnYEL 239
Cdd:COG0340    81 EALRELTGVDVGLKWPNDILLNGKKLAGILIEAS--GEGDGIDWVVIGIGINVNQPPFD-PEELDQPATSLKEETG-KEV 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 240 SRAALAAAMIRELDRMNDSWPSDK-SSYFNTYRDNCVTVGKNVDVTNyaTGAVRHGYAVDVNEDFSLKVRFENGDTEDVR 318
Cdd:COG0340   157 DREELLAALLEELEELYDRFLEEGfAPILEEWRARLATLGRRVRVET--GGETLEGIAVGIDEDGALLLETADGEIRAVA 234


                  ....*..
gi 1079632717 319 SGEVSVK 325
Cdd:COG0340   235 AGEVSLR 241
BirA COG1654
Biotin operon repressor [Transcription];
1-325 5.19e-66

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 210.61  E-value: 5.19e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717   1 MTKDEVLAILiKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGYRLISCPDRLYAGEIYAYLN-KERCS 79
Cdd:COG1654     4 STRLKLLRLL-ADGEFHSGEELAEELGVSRAAVWKHIKALRELGYEIESVPGKGYRLAEPPDLLDPEEIRAGLStKRLGR 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:COG1654    83 EILYVISSTSTNLLALELAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGGGLLYSLLLRPPIAPALLSLLLLAAAVAVA 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSVEGEIGMIsnIIVGIGVNVRQRPEDFPPELRETASSLGIYSGNYEL 239
Cdd:COG1654   163 AALAEGGGLVKWKKWPNDLLKKGKKILGILEEEGGDADGVVI--VVGGGGNNNNSNPEEEPQELAELATSLLLILRLRLL 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 240 SRAALAAAMIRELDRMNDSWPSDKSSYFNTYRDNCVTVGKNVDVTNYatGAVRHGYAVDVNEDFSLKVRFENGDTEDVRS 319
Cdd:COG1654   241 RLLLLLLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVVVEIG--GGGGGGGALGGGLLGLLLLGGGGGGGEGSLS 318


                  ....*.
gi 1079632717 320 GEVSVK 325
Cdd:COG1654   319 AVVVLL 324
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 80-254 1.15e-59

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 189.01  E-value: 1.15e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:cd16442     1 KLIVLDEIDSTNDEAKELARSGAPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLRPDVPPAEAPLLTLLAAVAVA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSVFDIRTDIKWVNDLFLNGRKITGILTEMSVEGEigMISNIIVGIGVNVRQRPEDFPPELRETASSLGIysgnyEL 239
Cdd:cd16442    81 EALEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGE--GVAAVVIGIGINVNNTPPPEPLPDTSLATSLGK-----EV 153

                         170
                  ....*....|....*
gi 1079632717 240 SRAALAAAMIRELDR 254
Cdd:cd16442   154 DRNELLEELLAALEN 168
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
1-324 7.83e-58

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 189.23  E-value: 7.83e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717   1 MTKDEVLAILiKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGYRLISCPDRLYAGEIYAYLNKERCSn 80
Cdd:PRK11886    4 TVMLQLLSLL-ADGDFHSGEQLGEELGISRAAIWKHIQTLEEWGLDIFSVKGKGYRLAEPLDLLDPERISSQLPPGRVT- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  81 iiCLPSVDSTNNILKsMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVHR 160
Cdd:PRK11886   82 --VLPVIDSTNQYLL-DRIAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWRLNQGPAQAMGLSLVVGIAIAE 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 161 AILSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRpeDFPPELRETA----SSLGIysgn 236
Cdd:PRK11886  159 ALRRLGAIDVGLKWPNDIYLNDRKLAGILVELS--GETGDAAHVVIGIGINVAMP--DFPEELIDQPwsdlQEAGP---- 230

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 237 yELSRAALAAAMIRELDR-MNDSWPSDKSSYFNTYRDNCVTVGKNVDVTnyaTGAVR-HGYAVDVNEDFSLKVRfENGDT 314
Cdd:PRK11886  231 -TIDRNQLAAELIKQLRAaLELFEQEGLAPFLERWKKLDLFLGREVKLI---IGDKEiSGIARGIDEQGALLLE-DDGVE 305

                         330
                  ....*....|
gi 1079632717 315 EDVRSGEVSV 324
Cdd:PRK11886  306 KPFNGGEISL 315
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 81-323 1.16e-42

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 147.16  E-value: 1.16e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  81 IICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADISEITCWTAVAVHR 160
Cdd:TIGR00121   2 VIVLDVIDSTNQYALELAKEGKLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILRPDLPKSPAPGLTLVAGIAIAE 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 161 AiLSVFDIRTDIKWVNDLFLNGRKITGILTEMSveGEIGMISNIIVGIGVNVRQRpeDFPPELRETASSLGIYSGnYELS 240
Cdd:TIGR00121  81 V-LKELGDQVQVKWPNDILLKDKKLGGILTELT--GKENRADYVVIGIGINVQNR--KPAESLREQAISLSEEAG-IDLD 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 241 RAALAAAMIRELD-RMNDSWPSDKSSYFNTYRDNCVTVGKNVDVTNYATGAVrhGYAVDVNEDFSLKVRfENGDTEDVRS 319
Cdd:TIGR00121 155 RGELIEGFLRNFEeNLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIE--GIARGIDKDGALLLE-DGGGIKKIIS 231


                  ....
gi 1079632717 320 GEVS 323
Cdd:TIGR00121 232 GEIS 235
PRK08330 PRK08330
biotin--protein ligase; Provisional
 80-325 5.73e-35

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 127.17  E-value: 5.73e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPpAGTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADISEITCWTAVAVH 159
Cdd:PRK08330    4 NIIYFDEVDSTNEYAKRIAPDEE-EGTVIVADRQTAGHGRKGRAWASPEG-GLWMSVILKPKVSPEHLPKLVFLGALAVV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAiLSVFDIRTDIKWVNDLFLNGRKITGILTEMSVEgeigmisNIIVGIGVNVRQrpeDFPPELRETASSLGIYSGNyEL 239
Cdd:PRK08330   82 DT-LREFGIEGKIKWPNDVLVNYKKIAGVLVEGKGD-------FVVLGIGLNVNN---EIPDELRETATSMKEVLGR-EV 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 240 SRAALAAAMIRELDRMNDSWPSDKSSYFNTYRDNCVTVGKNVDVTNYATGAVRhGYAVDVNEDFSLKVRFENGDTEDVRS 319
Cdd:PRK08330  150 PLIEVFKRLVENLDRWYKLFLEGPGEILEEVKGRSMILGKRVKIIGDGEILVE-GIAEDIDEFGALILRLDDGTVKKVLY 228


                  ....*.
gi 1079632717 320 GEVSVK 325
Cdd:PRK08330  229 GDVSLR 234
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 85-211 6.35e-18

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 78.64  E-value: 6.35e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  85 PSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADISEI---TCWTAVAVHRA 161
Cdd:pfam03099   3 ERIKSTNTYLEELNSSELESGGVVVVRRQTGGRGRGGNVWHSPKG-CLTYSLLLSKEHPNVDPSVLefyVLELVLAVLEA 81

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1079632717 162 ILSVF----DIRTDIKWVNDLFLNGRKITGILTEmSVEGEIgmISNIIVGIGVN 211
Cdd:pfam03099  82 LGLYKpgisGIPCFVKWPNDLYVNGRKLAGILQR-STRGGT--LHHGVIGLGVN 132
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 80-217 2.07e-13

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 68.74  E-value: 2.07e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  80 NIICLPSVDSTNNILKSMLPDQPPAGTCIIANEQTGGRGRMGRSFISPPNTgIYLSMLLrPQSAVAdiSEIT----CWTA 155
Cdd:PTZ00276    8 NIHFVGEVTSTMDVARTMLAAAGGKPFAVLAESQTAGRGTGGRTWTSPKGN-MYFTLCI-PQKGVP--PELVpvlpLITG 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1079632717 156 VAVHRAILSVFDIRT-DIKWVNDLFLNGRKITGILTEmsVEGEigmisNIIVGIGVNVRQRPE 217
Cdd:PTZ00276   84 LACRAAIMEVLHGAAvHTKWPNDIIYAGKKIGGSLIE--SEGE-----YLIIGIGMNIEVAPP 139
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
89-247 1.37e-11

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 64.03  E-value: 1.37e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  89 STNNILKSMLPDQP------PAGTCIIANEQTGGRGRMGRSFISPPNTGIYLSM---LLRPQSAVADISeITCWTAVAVH 159
Cdd:PRK06955   43 STNADLMARLKALPrsadalPAPIVRVAYEQTAGRGRQGRPWFAQPGNALLFSVacvLPRPVAALAGLS-LAVGVALAEA 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 160 RAILSV-FDIRTDIKWVNDLFLNGRKITGILTEmSVEGEIGMISNIIvGIGVNVRqRPEDFPPE---LRETASSLGiysg 235
Cdd:PRK06955  122 LAALPAaLGQRIALKWPNDLLIAGRKLAGILIE-TVWATPDATAVVI-GIGLNVR-RADAVAAEvdaLRAREAALA---- 194

                         170
                  ....*....|..
gi 1079632717 236 nYELSRAALAAA 247
Cdd:PRK06955  195 -RGLPPVALAAA 205
PRK05935 PRK05935
biotin--protein ligase; Provisional
 86-211 4.35e-10

biotin--protein ligase; Provisional


Pssm-ID: 235649 [Multi-domain]  Cd Length: 190  Bit Score: 58.29  E-value: 4.35e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  86 SVDSTNNILKSMLPD-QPPAGTCIIANEQTGGRGRMGRSFISpPNTGIYLSMLLRPQSAVADISEITCWTAVAVHRAILS 164
Cdd:PRK05935   10 ETPSTNTTAKEGMHLwDPYALTVISTREQTAGKGKFGKSWHS-SDQDLLASFCFFITVLNIDVSLLFRLGTEAVMRLGED 88

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 1079632717 165 VFDIRTDIKWVNDLFLNGRKITGILTE-MSVEGEIGmisnIIVGIGVN 211
Cdd:PRK05935   89 LGITEAVIKWPNDVLVHGEKLCGVLCEtIPVKGGLG----VILGIGVN 132
HTH_11 pfam08279
HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.
 6-55 1.90e-09

HTH domain; This family includes helix-turn-helix domains in a wide variety of proteins.


Pssm-ID: 429896 [Multi-domain]  Cd Length: 52  Bit Score: 52.82  E-value: 1.90e-09
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1079632717   6 VLAILIKKADYVSGESISSEIGVSRAAVNGAVKSLKADGCLIDSVTNKGY 55
Cdd:pfam08279   3 ILQLLLEARGPISGQELAEKLGVSRRTIRRDIKILEELGVPIEAEPGRGY 52
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
81-219 8.09e-09

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 54.96  E-value: 8.09e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  81 IICLPSVDSTNNILKSMLPD---QPPagTCIIANEQTGGRGRMGRSFISPPNtGIYLSMLLRPQSAVADI--SEITCWTA 155
Cdd:PRK08477    3 IRVFESLDSTQTYLIEKIKNgelKAP--FAIVAKEQTAGIGSRGNSWEGKKG-NLFFSFALKESDLPKDLplQSSSIYFG 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079632717 156 VaVHRAILSVFDIRTDIKWVNDLFLNGRKITGILTEMsvegeigMISNIIVGIGVNVRQRPEDF 219
Cdd:PRK08477   80 F-LLKEVLKELGSKVWLKWPNDLYLDDKKIGGVITNK-------IKNFIVCGIGLNLKFSPKNF 135
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
94-325 5.80e-07

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 50.86  E-value: 5.80e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717  94 LKSMLPDQPPAGTCIiANEQTGGRGRMGRSFISPPNTGIYLSM---LLRPQSAVADISEITcwtAVAVHRAiLSVFDIRT 170
Cdd:PRK13325  100 LARIAPDKAHKTICV-THLQSKGRGRQGRKWSHRLGECLMFSFgwvFDRPQYELGSLSPVA---AVACRRA-LSRLGLKT 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 171 DIKWVNDLFLNGRKITGILTEMSvegEIGMISNIIVGIGVNVRqrpedFPPELRETASSLGIYS-----GNYElsRAALA 245
Cdd:PRK13325  175 QIKWPNDLVVGRDKLGGILIETV---RTGGKTVAVVGIGINFV-----LPKEVENAASVQSLFQtasrrGNAD--AAVLL 244

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 246 AAMIRELDRMNDSWPSDK-SSYFNTYRDNCVTVGKNVDVTNYATgAVRHGYAVDVNEDFSLKVRFENGDtEDVRSGEVSV 324
Cdd:PRK13325  245 ETLLAELDAVLLQYARDGfAPFVAEYQAANRDHGKAVLLLRDGE-TVFEGTVKGVDGQGVLHLETAEGK-QTVVSGEISL 322


                  .
gi 1079632717 325 K 325
Cdd:PRK13325  323 R 323
PTZ00275 PTZ00275
biotin-acetyl-CoA-carboxylase ligase; Provisional
 108-218 2.44e-06

biotin-acetyl-CoA-carboxylase ligase; Provisional


Pssm-ID: 185536 [Multi-domain]  Cd Length: 285  Bit Score: 48.28  E-value: 2.44e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079632717 108 IIANEQTGGRG------RMGRSFISPPNTGIYLSMLLRPQSAVADISEITCWTAVAVHRAILSvFDIRTDIKWVNDLFLN 181
Cdd:PTZ00275   53 VSCNEQTNGIGtrdtkkNQDRIWLSEKGNLFTTFVFLWNRNDIEKVKYLAQTCTVAISKTLEY-FHLVTQIKWINDVLVN 131

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1079632717 182 GRKITGILTEMSVEGEIGMISN----IIVGIGVNVRQRPED 218
Cdd:PTZ00275  132 YKKIAGCLVHLYYLDDFPNLNSryvcVMVGIGINVTLEDKH 172
MntR COG1321
Mn-dependent transcriptional regulator MntR, DtxR family [Transcription];
5-57 4.75e-04

Mn-dependent transcriptional regulator MntR, DtxR family [Transcription];


Pssm-ID: 440932 [Multi-domain]  Cd Length: 135  Bit Score: 39.80  E-value: 4.75e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1079632717   5 EVLAILIKKADYVSGESISSEIGVSRAAVNGAVKSLKADGcLIDSVTNKGYRL 57
Cdd:COG1321    13 KAIYELSEEGGPVRTSDIAERLGVSPPSVTEMLKKLEEKG-LVEYEPYGGITL 64
BPL_LplA_LipB cd16435
biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), ...
 160-219 7.89e-03

biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.


Pssm-ID: 319740  Cd Length: 198  Bit Score: 37.13  E-value: 7.89e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1079632717 160 RAILSVFDIRTDIKWV-NDLFLNGRKITGILTEMSVEGeigmisnIIVGIGVNVRQRPEDF 219
Cdd:cd16435   108 RDAIADFGQSAEVKWGrNDLWIDNRKVCGIAVRVVKEA-------IFHGIALNLNQDLENF 161
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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