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Conserved domains on  [gi|1079631842|emb|SCW41293|]
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Anti-sigma regulatory factor (Ser/Thr protein kinase) [Ruminococcaceae bacterium YRB3002]

Protein Classification

ATP-binding protein( domain architecture ID 10005496)

ATP-binding protein with a histidine kinase-like ATPase domain, similar to serine/threonine-protein kinase BtrW, which phosphorylates and inactivates its specific antagonist protein BtrV and may function as a negative regulator of sigma-B activity

Gene Ontology:  GO:0005524|GO:0016787
PubMed:  16077112|12354223

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RsbW COG2172
Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];
3-135 3.86e-27

Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];


:

Pssm-ID: 441775 [Multi-domain]  Cd Length: 127  Bit Score: 97.68  E-value: 3.86e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842   3 ELDIKAVCENLPEVLSFVDEQLAHLSCSHKVISQIDAAVEEIFVNISSYAYdPMIGSATIRMEFSDDPLSVIISFVDGGV 82
Cdd:COG2172     1 SLSLPADLEDLGLARRAVRALLRELGLDEDDADDLVLAVSEAVTNAVRHAY-GGDPDGPVEVELELDPDGLEIEVRDEGP 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1079631842  83 PYDPLAKPDPnlkipVRDRRKGGLGIFIVKKTMDDVSYEYKDGQNILTIMKTL 135
Cdd:COG2172    80 GFDPEDLPDP-----YSTLAEGGRGLFLIRRLMDEVEYESDPGGTTVRLVKRL 127
 
Name Accession Description Interval E-value
RsbW COG2172
Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];
3-135 3.86e-27

Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];


Pssm-ID: 441775 [Multi-domain]  Cd Length: 127  Bit Score: 97.68  E-value: 3.86e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842   3 ELDIKAVCENLPEVLSFVDEQLAHLSCSHKVISQIDAAVEEIFVNISSYAYdPMIGSATIRMEFSDDPLSVIISFVDGGV 82
Cdd:COG2172     1 SLSLPADLEDLGLARRAVRALLRELGLDEDDADDLVLAVSEAVTNAVRHAY-GGDPDGPVEVELELDPDGLEIEVRDEGP 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1079631842  83 PYDPLAKPDPnlkipVRDRRKGGLGIFIVKKTMDDVSYEYKDGQNILTIMKTL 135
Cdd:COG2172    80 GFDPEDLPDP-----YSTLAEGGRGLFLIRRLMDEVEYESDPGGTTVRLVKRL 127
HATPase_c_2 pfam13581
Histidine kinase-like ATPase domain;
11-133 4.43e-24

Histidine kinase-like ATPase domain;


Pssm-ID: 433327 [Multi-domain]  Cd Length: 127  Bit Score: 89.66  E-value: 4.43e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  11 ENLPEVLSFVDEQLAHLSCSHKVISQIDAAVEEIFVNISSYAYDPMIGSaTIRMEFSDDPLSVIISFVDGGVPYDPLAKP 90
Cdd:pfam13581   6 EQLRAARRVLEAVLRRAGLPEELLDEVELAVGEACTNAVEHAYREGPEG-PVEVRLTSDGGGLVVTVADSGPPFDPLTLP 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1079631842  91 DPNLKIPVRDRRKGGLGIFIVKKTMDDVSYEYKDGQNILTIMK 133
Cdd:pfam13581  85 PPDLEEPDEDRKEGGRGLALIRGLMDDVEYTRGGEGNTVRMRK 127
HATPase_RsbW-like cd16936
Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase ...
 40-133 7.49e-20

Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase involved in regulating sigma-B during stress in Bacilli, and related domains; This family includes histidine kinase-like ATPase (HATPase) domain of RsbW, an anti sigma-B factor as well as a serine-protein kinase involved in regulating sigma-B during stress in Bacilli. The alternative sigma factor sigma-B is an important regulator of the general stress response of Bacillus cereus and B. subtilis. RsbW is an anti-sigma factor while RsbV is an anti-sigma factor antagonist (anti-anti-sigma factor). RsbW can also act as a kinase on RsbV. In a partner-switching mechanism, RsbW, RsbV, and sigma-B participate as follows: in non-stressed cells, sigma-B is present in an inactive form complexed with RsbW; in this form, sigma-B is unable to bind to RNA polymerase. Under stress, RsbV binds to RsbW, forming an RsbV-RsbW complex, and sigma-B is released to bind to RNA polymerase. RsbW may then act as a kinase on RsbV, phosphorylating a serine residue; RsbW is then released to bind to sigma-B, hence blocking its ability to bind RNA polymerase. A phosphatase then dephosphorylates RsbV so that it can again form a complex with RsbW, leading to the release of sigma-B.


Pssm-ID: 340413 [Multi-domain]  Cd Length: 91  Bit Score: 77.69  E-value: 7.49e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  40 AVEEIFVNISSYAYdPMIGSATIRMEFSDDPLSVIISFVDGGVPYDPLAKPDPNlkipvRDRRKGGLGIFIVKKTMDDVS 119
Cdd:cd16936     4 AVSEAVTNAVRHAY-RHDGPGPVRLELDLDPDRLRVEVTDSGPGFDPLRPADPD-----AGLREGGRGLALIRALMDEVG 77

                          90
                  ....*....|....
gi 1079631842 120 YEYKDGQNILTIMK 133
Cdd:cd16936    78 YRRTPGGKTVWLEL 91
PRK04069 PRK04069
serine-protein kinase RsbW; Provisional
 34-134 8.15e-06

serine-protein kinase RsbW; Provisional


Pssm-ID: 235217 [Multi-domain]  Cd Length: 161  Bit Score: 42.99  E-value: 8.15e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  34 ISQIDAAVEEIFVNISSYAY-DPMIGSATIRMEFSDDPLSVIISfvDGGVPYD------PLAKPDPNLkiPVRDRRKGGL 106
Cdd:PRK04069   40 IEDMKIAVSEACTNAVQHAYkEDEVGEIHIRFEIYEDRLEIVVA--DNGVSFDyetlksKLGPYDISK--PIEDLREGGL 115

                          90       100
                  ....*....|....*....|....*...
gi 1079631842 107 GIFIVKKTMDDVSYEYKDGqniLTIMKT 134
Cdd:PRK04069  116 GLFLIETLMDDVTVYKDSG---VTVSMT 140
 
Name Accession Description Interval E-value
RsbW COG2172
Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];
3-135 3.86e-27

Anti-sigma regulatory factor (Ser/Thr protein kinase) [Signal transduction mechanisms];


Pssm-ID: 441775 [Multi-domain]  Cd Length: 127  Bit Score: 97.68  E-value: 3.86e-27
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842   3 ELDIKAVCENLPEVLSFVDEQLAHLSCSHKVISQIDAAVEEIFVNISSYAYdPMIGSATIRMEFSDDPLSVIISFVDGGV 82
Cdd:COG2172     1 SLSLPADLEDLGLARRAVRALLRELGLDEDDADDLVLAVSEAVTNAVRHAY-GGDPDGPVEVELELDPDGLEIEVRDEGP 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1079631842  83 PYDPLAKPDPnlkipVRDRRKGGLGIFIVKKTMDDVSYEYKDGQNILTIMKTL 135
Cdd:COG2172    80 GFDPEDLPDP-----YSTLAEGGRGLFLIRRLMDEVEYESDPGGTTVRLVKRL 127
HATPase_c_2 pfam13581
Histidine kinase-like ATPase domain;
11-133 4.43e-24

Histidine kinase-like ATPase domain;


Pssm-ID: 433327 [Multi-domain]  Cd Length: 127  Bit Score: 89.66  E-value: 4.43e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  11 ENLPEVLSFVDEQLAHLSCSHKVISQIDAAVEEIFVNISSYAYDPMIGSaTIRMEFSDDPLSVIISFVDGGVPYDPLAKP 90
Cdd:pfam13581   6 EQLRAARRVLEAVLRRAGLPEELLDEVELAVGEACTNAVEHAYREGPEG-PVEVRLTSDGGGLVVTVADSGPPFDPLTLP 84

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1079631842  91 DPNLKIPVRDRRKGGLGIFIVKKTMDDVSYEYKDGQNILTIMK 133
Cdd:pfam13581  85 PPDLEEPDEDRKEGGRGLALIRGLMDDVEYTRGGEGNTVRMRK 127
HATPase_RsbW-like cd16936
Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase ...
 40-133 7.49e-20

Histidine kinase-like ATPase domain of RsbW, an anti sigma-B factor and serine-protein kinase involved in regulating sigma-B during stress in Bacilli, and related domains; This family includes histidine kinase-like ATPase (HATPase) domain of RsbW, an anti sigma-B factor as well as a serine-protein kinase involved in regulating sigma-B during stress in Bacilli. The alternative sigma factor sigma-B is an important regulator of the general stress response of Bacillus cereus and B. subtilis. RsbW is an anti-sigma factor while RsbV is an anti-sigma factor antagonist (anti-anti-sigma factor). RsbW can also act as a kinase on RsbV. In a partner-switching mechanism, RsbW, RsbV, and sigma-B participate as follows: in non-stressed cells, sigma-B is present in an inactive form complexed with RsbW; in this form, sigma-B is unable to bind to RNA polymerase. Under stress, RsbV binds to RsbW, forming an RsbV-RsbW complex, and sigma-B is released to bind to RNA polymerase. RsbW may then act as a kinase on RsbV, phosphorylating a serine residue; RsbW is then released to bind to sigma-B, hence blocking its ability to bind RNA polymerase. A phosphatase then dephosphorylates RsbV so that it can again form a complex with RsbW, leading to the release of sigma-B.


Pssm-ID: 340413 [Multi-domain]  Cd Length: 91  Bit Score: 77.69  E-value: 7.49e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  40 AVEEIFVNISSYAYdPMIGSATIRMEFSDDPLSVIISFVDGGVPYDPLAKPDPNlkipvRDRRKGGLGIFIVKKTMDDVS 119
Cdd:cd16936     4 AVSEAVTNAVRHAY-RHDGPGPVRLELDLDPDRLRVEVTDSGPGFDPLRPADPD-----AGLREGGRGLALIRALMDEVG 77

                          90
                  ....*....|....
gi 1079631842 120 YEYKDGQNILTIMK 133
Cdd:cd16936    78 YRRTPGGKTVWLEL 91
PRK04069 PRK04069
serine-protein kinase RsbW; Provisional
 34-134 8.15e-06

serine-protein kinase RsbW; Provisional


Pssm-ID: 235217 [Multi-domain]  Cd Length: 161  Bit Score: 42.99  E-value: 8.15e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1079631842  34 ISQIDAAVEEIFVNISSYAY-DPMIGSATIRMEFSDDPLSVIISfvDGGVPYD------PLAKPDPNLkiPVRDRRKGGL 106
Cdd:PRK04069   40 IEDMKIAVSEACTNAVQHAYkEDEVGEIHIRFEIYEDRLEIVVA--DNGVSFDyetlksKLGPYDISK--PIEDLREGGL 115

                          90       100
                  ....*....|....*....|....*...
gi 1079631842 107 GIFIVKKTMDDVSYEYKDGqniLTIMKT 134
Cdd:PRK04069  116 GLFLIETLMDDVTVYKDSG---VTVSMT 140
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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