NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1086933017|emb|SDO98135|]
View 

magnesium transporter [Arthrobacter sp. ok909]

Protein Classification

magnesium transporter CorA family protein( domain architecture ID 10191267)

magnesium transporter CorA family protein belonging to the CorA metal ion transporter (MIT) superfamily, similar to CorA which mediates both influx and efflux of magnesium, and may also transport nickel and cobalt

CATH:  3.30.460.20|1.20.58.340
Gene Ontology:  GO:0046873|GO:0030001
PubMed:  17619822|23046658|16179994
SCOP:  4000404|3000358

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 37-326 1.89e-103

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 304.88  E-value: 1.89e-103
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVTVH- 115
Cdd:cd12822     1 WIDLEGPTEEELQLLAEEFGFHPLAIEDALNERQRPKIEEYDDYLFLVLRALRYDEEELELETSELSIFLGKNFLVTVHe 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 116 GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDHdlQRKTFELRKNLVR 195
Cdd:cd12822    81 GPLRLIDEVIERLLSDPKLLPKGPDFLLYAILDAIVDSYFPILDELEDRLDELEDEILEEPRREV--LRELFALRRELLR 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 196 LRRVVLPMREVLNTLLRRDDLVGcSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSWAA 275
Cdd:cd12822   159 LRRILSPQREVLNRLLRRENELI-SEELRPYFRDVYDHLERLAELLDSYRDLLSSLLDAYLSLQSNRTNEVMKVLTIITT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1086933017 276 IIAVPTAVTGFFGQNVPFFGF-QSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12822   238 IFLPLTFITGIYGMNFEYMPElHWPYGYFIVLGIMLLIAGGLLWWFRRKKWL 289
 
Name Accession Description Interval E-value
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 37-326 1.89e-103

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 304.88  E-value: 1.89e-103
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVTVH- 115
Cdd:cd12822     1 WIDLEGPTEEELQLLAEEFGFHPLAIEDALNERQRPKIEEYDDYLFLVLRALRYDEEELELETSELSIFLGKNFLVTVHe 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 116 GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDHdlQRKTFELRKNLVR 195
Cdd:cd12822    81 GPLRLIDEVIERLLSDPKLLPKGPDFLLYAILDAIVDSYFPILDELEDRLDELEDEILEEPRREV--LRELFALRRELLR 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 196 LRRVVLPMREVLNTLLRRDDLVGcSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSWAA 275
Cdd:cd12822   159 LRRILSPQREVLNRLLRRENELI-SEELRPYFRDVYDHLERLAELLDSYRDLLSSLLDAYLSLQSNRTNEVMKVLTIITT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1086933017 276 IIAVPTAVTGFFGQNVPFFGF-QSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12822   238 IFLPLTFITGIYGMNFEYMPElHWPYGYFIVLGIMLLIAGGLLWWFRRKKWL 289
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
21-326 2.89e-93

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 279.41  E-value: 2.89e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  21 RAHEIEALAHQDDVVlWIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIA 100
Cdd:COG0598     2 ELDALEELLDPEGFV-WIDLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLRTLNYNEEEEELETG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 101 EVSAFVTRNALVTVH-GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDhsPD 179
Cdd:COG0598    81 PLSIFLGPNFLITVReRPLDAFDDVRERLERGPGLLPRGPDYLLYALLDAIVDRYFPVLEELEDELDELEDELFES--PD 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 180 HDLQRKTFELRKNLVRLRRVVLPMREVLNTLLRRDDLvGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQ 259
Cdd:COG0598   159 NELLRRLYRLRRELVRLRRALAPLREVLSRLLRGESP-LISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLI 237

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1086933017 260 GNQMNLVMKKVTSWAAIIAVPTAVTGFFGQNVPFF-GFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:COG0598   238 SNRQNEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMpELDWPYGYPIVLGLMLLIAVLLYLYFKRKGWL 305
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 32-322 1.47e-58

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 190.22  E-value: 1.47e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  32 DDVVLWIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNAL 111
Cdd:pfam01544   1 EQGFVWLDLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPGVNLDDSEEADELVTVSILLGDNFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 112 VTVH-GPGFDTDKLMAHWDAEtDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDdhSPDHDLQRKTFELR 190
Cdd:pfam01544  81 ITVRhEPLPALDEVRKRLTLL-GGGPLDPGDLLYRLLDEIVDRFLELLEKLEDELDELEDELED--ETNNELLRELAALR 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 191 KNLVRLRRVVLPMREVLNTLLRRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKV 270
Cdd:pfam01544 158 RSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNRVMKVL 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1086933017 271 TSWAAIIAVPTAVTGFFGQNV---PFFGFQSDYGLWLSTALMVTGSVFLYLGFKR 322
Cdd:pfam01544 238 TVVTAIFLPLTLITGIYGMNFgglPELEWSYGYPFVLILGLMILLAILLLFYFRR 292
corA TIGR00383
magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp ...
 32-326 2.53e-41

magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp Genomics 1998;3(3):151-69 discusses this family and suggests that some members may have functions other than Mg2+ transport. [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273048 [Multi-domain]  Cd Length: 318  Bit Score: 145.99  E-value: 2.53e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  32 DDVVLWIDFTDPTADDLL-GVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYqAGLRPGSGEVDIAEVSAFVTRNA 110
Cdd:TIGR00383  24 LNTVLWIDLIEPTDEERLaKLGQFFAIHPLALEDILNSPQRPKVEEDEDHLFIISF-FLNEDEDDTFETEQVSFILGKNL 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 111 LVTVHG---PGFDtdKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFddHSPDHDLQRKTF 187
Cdd:TIGR00383 103 LFTIHErelPAFD--SIRERIRTSQKVFEKGADYLLYDIFDAIIDSYFPLLENIEDELEELEDEII--SGPTSTLMDEIL 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 188 ELRKNLVRLRRVVLPMREVLNTLLRRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVM 267
Cdd:TIGR00383 179 SLRTELLALRRSLWPLRDVLNFLLRKTHLPIQTEEVREYLRDIYDHILSLLEMIETYRELLSSLMDLYLSLVNNKMNEIM 258

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 268 KKVTSWAAIIAVPTAVTGFFGQNVPFFG-FQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:TIGR00383 259 KILTVVSTIFIPLTFIAGIYGMNFKFMPeLNWKYGYPAVLIVMAVIALGPLIYFRRKGWL 318
zntB PRK09546
zinc transporter ZntB;
148-326 5.91e-03

zinc transporter ZntB;


Pssm-ID: 181941  Cd Length: 324  Bit Score: 38.06  E-value: 5.91e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 148 DMIVDGHFDAVQELDGYIDALEESLFDDHSPDhdlQRKTFELRKNLVRLRRVVLPMREVLNTL-------LRRDDLVGcs 220
Cdd:PRK09546  149 DALTDHASEFIEELHDKIIDLEDNLLDQQIPP---RGELALLRKQLIVMRRYMAPQRDVFARLaserlpwMSDDDRRR-- 223

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 221 pamqpfLQDVYDhvlRATEWTESLRDLV--TTILETHISiqgNQMNLVMKKVT---SWAAIIAVPTA-VTGFFGQN---V 291
Cdd:PRK09546  224 ------MQDIAD---RLGRGLDDLDACIarTAVLADEIA---SVMAEAMNRRTytmSLMAMVFLPTTfLTGLFGVNlggI 291

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1086933017 292 PffGFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:PRK09546  292 P--GGGWPFGFSIFCLLLVVLIGGVAWWLKRSKWL 324
 
Name Accession Description Interval E-value
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 37-326 1.89e-103

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 304.88  E-value: 1.89e-103
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVTVH- 115
Cdd:cd12822     1 WIDLEGPTEEELQLLAEEFGFHPLAIEDALNERQRPKIEEYDDYLFLVLRALRYDEEELELETSELSIFLGKNFLVTVHe 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 116 GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDHdlQRKTFELRKNLVR 195
Cdd:cd12822    81 GPLRLIDEVIERLLSDPKLLPKGPDFLLYAILDAIVDSYFPILDELEDRLDELEDEILEEPRREV--LRELFALRRELLR 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 196 LRRVVLPMREVLNTLLRRDDLVGcSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSWAA 275
Cdd:cd12822   159 LRRILSPQREVLNRLLRRENELI-SEELRPYFRDVYDHLERLAELLDSYRDLLSSLLDAYLSLQSNRTNEVMKVLTIITT 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1086933017 276 IIAVPTAVTGFFGQNVPFFGF-QSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12822   238 IFLPLTFITGIYGMNFEYMPElHWPYGYFIVLGIMLLIAGGLLWWFRRKKWL 289
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
21-326 2.89e-93

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 279.41  E-value: 2.89e-93
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  21 RAHEIEALAHQDDVVlWIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIA 100
Cdd:COG0598     2 ELDALEELLDPEGFV-WIDLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLRTLNYNEEEEELETG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 101 EVSAFVTRNALVTVH-GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDhsPD 179
Cdd:COG0598    81 PLSIFLGPNFLITVReRPLDAFDDVRERLERGPGLLPRGPDYLLYALLDAIVDRYFPVLEELEDELDELEDELFES--PD 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 180 HDLQRKTFELRKNLVRLRRVVLPMREVLNTLLRRDDLvGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQ 259
Cdd:COG0598   159 NELLRRLYRLRRELVRLRRALAPLREVLSRLLRGESP-LISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLI 237

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1086933017 260 GNQMNLVMKKVTSWAAIIAVPTAVTGFFGQNVPFF-GFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:COG0598   238 SNRQNEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMpELDWPYGYPIVLGLMLLIAVLLYLYFKRKGWL 305
MtCorA-like cd12830
Mycobacterium tuberculosis CorA-like subfamily; This bacterial subfamily belongs to the ...
 36-326 1.01e-80

Mycobacterium tuberculosis CorA-like subfamily; This bacterial subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Mg2+ transporter Mycobacterium tuberculosis CorA (which also transports Co2+). Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213364 [Multi-domain]  Cd Length: 292  Bit Score: 247.05  E-value: 1.01e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  36 LWIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVTV- 114
Cdd:cd12830     3 VWVGLHEPDAEELAAVAEEFGLHELAVEDAVHAHQRPKLERYGDSLFLVLRTARYVDHTEEVETGETHVFVGPDFVVTVr 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 115 HGPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDhDLQRkTFELRKNLV 194
Cdd:cd12830    83 HGAAGPLARVRARLEADPELLALGPDAVLYAILDFIVDGYLPVAEELEDDVDELEEEVFSPDRRD-DIER-IYRLKREVL 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 195 RLRRVVLPMREVLNTLLRRDDLVGCsPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSWA 274
Cdd:cd12830   161 EFRRAVDPLVEVLRRLARGHEPLIP-KELRPYFRDVADHLLRVADRVDSLRELLSSALQANLALVSVQQNEDMRKISAWA 239

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1086933017 275 AIIAVPTAVTGFFGQNvpffgFQ------SDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12830   240 AILAVPTAIAGIYGMN-----FDhmpelhWTYGYPLVLGLMVAVCLGLYRRFRRNGWL 292
TmCorA-like_1 cd12828
Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima ...
 35-326 1.80e-59

Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this subfamily are found in all three kingdoms of life. It is functionally diverse subfamily, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes Methanosarcina mazei CorA which may be involved in transport of copper and/or other divalent metal ions. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213362 [Multi-domain]  Cd Length: 294  Bit Score: 192.64  E-value: 1.80e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  35 VLWIDFTDPTADDLLG-VETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVT 113
Cdd:cd12828     2 VTWINVDGLHDIELIEkLGERFGLHPLVLEDILNTGQRPKVEEYDDYLFIVLKMLYYDEDTGELESEQVSLILGKNYVIS 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 114 VH---GPGFD--TDKLMAHwdaETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDhsPDHDLQRKTFE 188
Cdd:cd12828    82 FQerpGDVFDpvRERLRNG---KGRIRKKGADYLAYALLDAIVDNYFPVLEKLGDRIEDLEEELLEN--PTPETLERIHK 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 189 LRKNLVRLRRVVLPMREVLNTLLRRD-DLVgcSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVM 267
Cdd:cd12828   157 LKRELLFLRRSIWPLREVINRLLRGEsPLI--SEETRPYLRDVYDHTIQVIDTIETYRDMLSGLLDLYLSSVSNRMNEVM 234

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1086933017 268 KKVTSWAAIIAVPTAVTGFFGQN---VPFFGFQsdYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12828   235 KVLTIISTIFIPLTFIAGIYGMNfeyMPELEWK--YGYPAVLGVMLLIALGMLLYFKRKKWL 294
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 32-322 1.47e-58

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 190.22  E-value: 1.47e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  32 DDVVLWIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNAL 111
Cdd:pfam01544   1 EQGFVWLDLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPGVNLDDSEEADELVTVSILLGDNFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 112 VTVH-GPGFDTDKLMAHWDAEtDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDdhSPDHDLQRKTFELR 190
Cdd:pfam01544  81 ITVRhEPLPALDEVRKRLTLL-GGGPLDPGDLLYRLLDEIVDRFLELLEKLEDELDELEDELED--ETNNELLRELAALR 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 191 KNLVRLRRVVLPMREVLNTLLRRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKV 270
Cdd:pfam01544 158 RSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNRVMKVL 237

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1086933017 271 TSWAAIIAVPTAVTGFFGQNV---PFFGFQSDYGLWLSTALMVTGSVFLYLGFKR 322
Cdd:pfam01544 238 TVVTAIFLPLTLITGIYGMNFgglPELEWSYGYPFVLILGLMILLAILLLFYFRR 292
TmCorA-like_u2 cd12831
Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This ...
 37-325 4.72e-48

Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213365 [Multi-domain]  Cd Length: 287  Bit Score: 162.72  E-value: 4.72e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEvdiaEVSAFVTRNALVTVHG 116
Cdd:cd12831     4 WVDFNNPTEEEIKLLTSFFHFHPLAIEDCLHLLQRPKLDYYDDYQFLVLHAIDQEDLEAE----EVNLFVGENYIVTFHK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 117 PGFDT-DKLMAHWDAETDLaAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESlfDDHSPDHDLQRKTFELRKNLVR 195
Cdd:cd12831    80 EPIREiNEVWEKLKEKKDK-KLGTLYIAYMIIDKIVDDYFPIVYEIEDRLNEIEDN--NVDSSNKKLMDEVFDIRSDLLK 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 196 LRRVVLPMREVLNTLLRRDDLVGCSPAMQPFlQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSWAA 275
Cdd:cd12831   157 LRRTILPMRDLLYRILNSERLEGDKEQRAYF-QDIYDHLLKLSEMLESNREMTADIRDNYESYNSNRMNSIMMTLTIIST 235

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1086933017 276 IIAVPTAVTGFFGQNvpfFGF----QSDYGLWLSTALMVTGSVFLYLGFKRRDW 325
Cdd:cd12831   236 IFMPLTFIAGIYGMN---FEYmpelKWHYGYFVVLGIMLLIAIGMILWFKKKGW 286
corA TIGR00383
magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp ...
 32-326 2.53e-41

magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp Genomics 1998;3(3):151-69 discusses this family and suggests that some members may have functions other than Mg2+ transport. [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273048 [Multi-domain]  Cd Length: 318  Bit Score: 145.99  E-value: 2.53e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  32 DDVVLWIDFTDPTADDLL-GVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYqAGLRPGSGEVDIAEVSAFVTRNA 110
Cdd:TIGR00383  24 LNTVLWIDLIEPTDEERLaKLGQFFAIHPLALEDILNSPQRPKVEEDEDHLFIISF-FLNEDEDDTFETEQVSFILGKNL 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 111 LVTVHG---PGFDtdKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFddHSPDHDLQRKTF 187
Cdd:TIGR00383 103 LFTIHErelPAFD--SIRERIRTSQKVFEKGADYLLYDIFDAIIDSYFPLLENIEDELEELEDEII--SGPTSTLMDEIL 178

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 188 ELRKNLVRLRRVVLPMREVLNTLLRRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVM 267
Cdd:TIGR00383 179 SLRTELLALRRSLWPLRDVLNFLLRKTHLPIQTEEVREYLRDIYDHILSLLEMIETYRELLSSLMDLYLSLVNNKMNEIM 258

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 268 KKVTSWAAIIAVPTAVTGFFGQNVPFFG-FQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:TIGR00383 259 KILTVVSTIFIPLTFIAGIYGMNFKFMPeLNWKYGYPAVLIVMAVIALGPLIYFRRKGWL 318
MIT_CorA-like cd11744
metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of ...
 37-325 2.85e-25

metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. They are found in most bacteria and archaea, and in some eukaryotes. It is a functionally diverse group which includes the Mg2+ transporters of Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and the Zn2+ transporter Salmonella typhimurium ZntB, which mediates the efflux of Zn2+ (and Cd2+). It includes five Saccharomyces cerevisiae members: i) two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, ii) two mitochondrial inner membrane Mg2+ transporters: Mfm1p/Lpe10p, and Mrs2p, and iii) and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. It also includes a family of Arabidopsis thaliana members (AtMGTs), some of which are localized to distinct tissues, and not all of which can transport Mg2+. Thermotoga maritima CorA and Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, Mrs2p, and Alr1p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213354 [Multi-domain]  Cd Length: 286  Bit Score: 102.67  E-value: 2.85e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVTVH- 115
Cdd:cd11744     1 WIHCERLHDDDIRRVLEDNLHVPRALEDILLAESRPSFHPLDDNFMIILRGFNMDENASPEDMLSVRILYFQGALISTRe 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 116 --GPGFDTDKLMAHWDAETDLAAHgvpFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSpDHDLQRkTFELRKNL 193
Cdd:cd11744    81 ipSRAFMEIRQRLREHSKGPKSAA---SLLNQLIEGLNGKIDLYLETIEETLNELDVNDEEGHY-DESTYN-HIALQKAL 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 194 VRLRRVVLPMREVLNTLLRRD-DLVGCSPamqPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTS 272
Cdd:cd11744   156 ISIRRFIRPQQYALRDLIRSEsELVTSRP---HQYRFAHNNITRINETIEFYLGEVALLQDEIKHNRDEKTNKNSKLFTL 232

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 1086933017 273 WAAIIAVPTAVTGFFGQNVPFFG--FQSDYGLWLSTALMVTGsVFLYLGFKRRDW 325
Cdd:cd11744   233 VATIFLPTTFLAGLYGMNFEGMPgvESSMAFTWFCIALIVAF-GLEWLLFKRKGF 286
Alr1p-like cd12829
Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the ...
 37-317 5.45e-24

Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213363 [Multi-domain]  Cd Length: 305  Bit Score: 99.65  E-value: 5.45e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLF--LSAYQAGLRPGSGEVDIAEVSAFVTRNALVTV 114
Cdd:cd12829     1 WLDVQSPTDDDMRAIGKLFPIHPLTLEDILSQDTREKVELFPNYYFisFRAIESDSELDENVLEPVNVYIVVFREGILTF 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 115 HGPGFD-TDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDH-DLQRKTFELRKN 192
Cdd:cd12829    81 HFKPLDhIERVRKRIRLLSDSHLLSSDWVLYALLDSIVDSFFPLVDSIEKEVDSIDELVLLISSSDQsDLLRRIGDLRRR 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 193 LVRLRRVVLPMREVLNTLLRR---DDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISI-------QGNQ 262
Cdd:cd12829   161 VTSLLRLLFAKSDVLKQLLKRlmkKTFVSPDSEVAIYLGDVQDHILTLLQKLNHYEDLLSRSHSNYLAQlslemsqESNG 240

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1086933017 263 MNLVMKKVTSwAAIIAVP-TAVTGFFGQNV--PFFGFQSDYGLW-----LSTALMVTGSVFLY 317
Cdd:cd12829   241 TDKAMLRLTV-IATIFLPlQLITGLFGMNVkvPGQDIESLYWFFgivivAIMVLVAIVVVFLV 302
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 37-326 3.10e-21

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 91.41  E-value: 3.10e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANH---LFLSAYQAGLRPGSGEVDIAEVSAFVTRNALVT 113
Cdd:cd12827     1 WINVVNPTEEEIEQLAEKYGIPEDFLTYALDEEERSRIEYDDDTdylLIILDVPVEEEEDDDEYETIPLGIILTKDRLIT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 114 V--------------HGPGFDTDKLMAhwdaetdlaahgvpFLFwGLLDMIVDGHFDAVQELDGYIDALEESLfdDHSPD 179
Cdd:cd12827    81 IcnhetdllddflegRVKNFDTSSKTR--------------FLL-QILYRIATSYLKYLRQINKERDELEQEL--RKSTK 143

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 180 -HDLQRkTFELRKNLVRLR------RVVLpmrevlnTLLRRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTIL 252
Cdd:cd12827   144 nKELFQ-LLDLEKSLVYFStslksnEIVL-------EKLKRSRSLKLDEEDKELLEDVIIENKQAIEMAEIYSDILSGLM 215

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1086933017 253 ETHISIQGNQMNLVMKKVTSWAAIIAVPTAVTGFFGQNVPFFGFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12827   216 DAFASVISNNLNNVMKFLTSITIVLTIPTIITGFYGMNVPLPFADSPYAFWIILLISLLLSVIVALILRRKKYF 289
TmCorA-like_u3 cd12832
Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs ...
 37-326 2.99e-20

Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213366 [Multi-domain]  Cd Length: 287  Bit Score: 88.81  E-value: 2.99e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSAYQAGLRPGSGEVDIAEVSA----FVTRNALV 112
Cdd:cd12832     1 WIDVVAPTEEDLEDLARRYNLHETSLKDCLDPDHLPKYEEVDGVTFIILRVYTDKKRENADTIQELTTkiaiFVVDKFVI 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 113 TVH---GPGFDTdkLMAHWDAETdLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDhDLQRKTFEL 189
Cdd:cd12832    81 TIHrtdLEFIAN--LKEKWLSGM-HKCESPTELLLRIVKDALKTYEAPLEKLSEELDKYEEEVFLKKRKG-SLLKRLYHL 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 190 RKNLVRLRRVVLPMREVLNTLlrrdDLVGCSPamqPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKK 269
Cdd:cd12832   157 KRKASVIKRVLNLTQDVIDKI----DTPLKSD---PYTQDLRDLLIKLLSLYDELHENATNLLSLYISLSSQRTNEVMRV 229

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1086933017 270 VTswaaIIAV---P-TAVTGFFGQNVPFFG-FQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12832   230 LT----IFSVfflPlTFIAGVYGMNFKYMPeLHWKYGYPATLLVMIVIVVGIYFWFKRKGWL 287
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 37-325 4.77e-20

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 88.14  E-value: 4.77e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPT-ADDLLGVETVYGFHRLAVEDAVHDFQRPKLDR-YANHLFLSayqagLRPGSGEVDIAEVSAFVTRNALVTV 114
Cdd:cd12821     1 WYDIDEPDdEIRRLLEELESSHLPNDLRDILLGVSFPRFEEvSGSGIYIQ-----LRKPQKDADTASFHFLITRDRLITV 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 115 H-GPGFDTDKLMAHWDAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLfdDHSPDHDLQRKTFELRKNL 193
Cdd:cd12821    76 RlDELPALDELRQDLRSGQMKSAKSAAELLFVLLGAIIKALLTGIDQFEEKLEELEWDL--LEGNNAIKLDRILELRREL 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 194 VRLRRVVLPMREVLNTLLRrDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQMNLVMKKVTSW 273
Cdd:cd12821   154 LRLTNLIEPQQEVLMALQE-AFAELLFSEDEEELRRTLDRIERLLQLIEEYEQELDTLQDIEEVVSSHRGNEIMKTLTVV 232

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1086933017 274 AAIIAVPTAVTGFFGQNVPF-FGFQSDYGLWLSTALMVTGSVFLYLGFKRRDW 325
Cdd:cd12821   233 TTVFLPLTLIGGLWGMNFKNmPELEWKYGYLFVLGLIILSTAGLYLYLRRKGW 285
ZntB-like_1 cd12833
Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to ...
 36-326 5.51e-18

Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213367 [Multi-domain]  Cd Length: 290  Bit Score: 82.54  E-value: 5.51e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  36 LWI--DFTDPTADDLLgvETVYGFHRLAVEDAVHDFQRPKLDRYANHLFLSayqagLR-----PGSGEVDIAEVSAFVTR 108
Cdd:cd12833     3 LWLhlDYSDPEAQDWL--EQSSGLPELVIDALLAEETRPRATRLGDGLLLN-----LRgvnlnPGADPEDMVSIRLWITP 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 109 NALVTVHgpgfdTDKLMAHWDAETDLAAHGVP-----FLFWgLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDhdLQ 183
Cdd:cd12833    76 GRIISTR-----RRRLLAVDDIREALEAGKGPkspgdFLAA-LAERLTDRMEDVIDELEDRLDELEERVLEEEDEE--LR 147

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 184 RKTFELRKNLVRLRRVVLPMREVLNTLLrRDDLVGCSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILETHISIQGNQM 263
Cdd:cd12833   148 GELAELRRQAIALRRYLAPQRDALERLA-REDLPWLSDDDRLRLREAADRLTRYIEDLDAIRERAAVLQEELTNRRAEQM 226

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1086933017 264 NLVMKKVTSWAAIIAVPTAVTGFFGQNV---PffGFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12833   227 NRRMYVLSIVAAIFLPLGFLTGLLGINVggiP--GAENPWAFWIFCGLLVVLAVGLLLLFRRKKWL 290
EcCorA-like_u1 cd12837
uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A ...
 36-326 1.54e-10

uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A uncharacterized subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA_ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA_ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213371 [Multi-domain]  Cd Length: 298  Bit Score: 61.01  E-value: 1.54e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  36 LWIDFTDPTADDLLGVETVYGFH--------------RLAVEDavhdfqrpkldryaNHLFLSAYQAGlRPGSGEVDIAE 101
Cdd:cd12837     1 VWIDLLNPTPEEEAAVEALLGIAiptreemseieassRLYREG--------------GALYMTAPLLS-RADTDDPELSP 65

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 102 VSAFVTRNALVTV--HGPG-FDT--DKLmahwdAETDLAAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDH 176
Cdd:cd12837    66 VTFVLTPERLVTVryADPRaFDTfaARA-----ARQPSGCASGEDVLLGLLEAIVDRLADVLERVGADLDRLSRRIFRSS 140

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 177 SPDHDlqRKTFELRKNLVRLRR---VVLPMREVLNTLLRrddlvgcspaMQPFLQDVYDHVLRAT--EWTESL-RDLVTt 250
Cdd:cd12837   141 AKRRR--RASRDLRSVLRRIGRagdLLSKIRESLLSLGR----------LLSFLSANAADWLSKElrARLKTLqRDVAS- 207

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 251 iLETHISIQGNQMNLVMK--------------KVTSWAAIIAV-PTAVTGFFGQNVPFF-GFQSDYGLWLSTALMVTGSV 314
Cdd:cd12837   208 -LSEHATFLSNKIQFLLDatlglinieqnniiKIFSVASVVFLpPTLIASIYGMNFKHMpELDWPFGYPLALGLMVLSAV 286

                         330
                  ....*....|..
gi 1086933017 315 FLYLGFKRRDWI 326
Cdd:cd12837   287 LPLLWFKRRGWL 298
ZntB_u1 cd12834
Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like ...
 142-323 4.23e-10

Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; The MIT superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. The ZntB-like subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213368 [Multi-domain]  Cd Length: 290  Bit Score: 59.52  E-value: 4.23e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 142 LFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSpdHDLQRKTFELRKNLVRLRRVVLPMREVLNTLLRRDDLvGCSP 221
Cdd:cd12834   108 LLEHLLREQADTLSRIVRELTDELDRIEDRLLSGRL--RRERRELGALRRRLVRLHRLLAPEPAALFRLLRRPPD-WLPD 184

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 222 AMQPFLQdvydhvlRATEWTESL-RDLV-----TTILETHISIQGN-QMNLVMKKVTSWAAIIAVPTAVTGFFGQNVP-F 293
Cdd:cd12834   185 ADLETLR-------RLTERLDALdRDLValqerARLLQEEIAARLAeQTNRSLYILSVVTALLLPPTLVTGLFGMNTGgL 257

                         170       180       190
                  ....*....|....*....|....*....|
gi 1086933017 294 FGFQSDYGLWLSTALMVTGSVFLYLGFKRR 323
Cdd:cd12834   258 PLAEGPHGFWIAVALALLAAGLAYLLLRRL 287
HpCorA-like cd12836
Mg2+ transporter Helicobacter pylori CorA-like subgroup; A bacterial subgroup of the ...
 35-326 7.61e-08

Mg2+ transporter Helicobacter pylori CorA-like subgroup; A bacterial subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Mg2+ transporter Helicobacter pylori CorAs (which can also transport Co2+, and Ni2+); CorA plays an important role in the viability of this pathogen. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB (members of the EcCorA_ZntB-like family) form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213370 [Multi-domain]  Cd Length: 288  Bit Score: 52.65  E-value: 7.61e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  35 VLWIDFTDPTADDLLGVETVYGF------HRLAVEDAvhdfqrpklDRY--------ANHLFLSayqaglrpGSGEVDIA 100
Cdd:cd12836     1 VLWIDLINPTEEEIKTVEQYFNIefptkqESEEIESS---------SRYwededsitINSNFLV--------PDDDGYHN 63

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 101 E-VSAFVTRNALVTVHGPGFDT-----DKLMAHWDAETDlAAHgvpfLFWGLLDMIVDGHFDAVQELDGYIDALEESLFD 174
Cdd:cd12836    64 EpVSFILKDNILFTIRYGELRTfsetsKKLRANPKKFQT-GYD----IFSSILEIRIDYDADLLENISKEISKLRKKLFA 138

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 175 DHSPDHDLQRKTFELRKNLVRLRRVVLPMREVLNTLLRRDDlvgcspamqpFLQDVYDHVLRATEWTESLRDLVTTILET 254
Cdd:cd12836   139 DEDLDEELLEKISRLQELNMTIRENLIDKQRVLSSLLKSEK----------FPEDLKENLRIMIKDINSLIEHTTFNFER 208

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 255 ----------HISIQGNQmnlVMKKVTSWAAIIAVPTAVTGFFGQNvpfFGFQSD----YGLWLSTALMVTGSVFLYLGF 320
Cdd:cd12836   209 ldylqntflgLINIEQNK---IIKIFTVATVIFMPPTLIASIYGMN---FKFMPEldwkYGYPFALLLMVISSILPLLYF 282


                  ....*.
gi 1086933017 321 KRRDWI 326
Cdd:cd12836   283 KKKKWL 288
EcCorA-like cd12825
Escherichia coli Mg2+ transporter CorA_like subfamily; A bacterial subfamily of the ...
 37-326 1.56e-06

Escherichia coli Mg2+ transporter CorA_like subfamily; A bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB_like(EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Mg2+ transporters Escherichia coli, Salmonella typhimurium, and Helicobacter pylori CorAs (which can also transport Co2+, and Ni2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213359 [Multi-domain]  Cd Length: 287  Bit Score: 48.85  E-value: 1.56e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  37 WIDFTDPTADDLLGVETVYGFHrLAVEDAVHDFQ-----RPKLDRYANHLFLSAYQAglrpgsgEVDIAEVSAFVT--RN 109
Cdd:cd12825     1 WIDLINPTEEELATLSSEYGFI-LPPREHLEDIEissryWEDDSQLFITLSFPNQTK-------EDTIEEEPVTFIikGN 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 110 ALVTVHG---PGFDTDKLMAHWDAETDLAAHGvpfLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHSPDHDLQRKT 186
Cdd:cd12825    73 ILFTIRYrdlPAFDIFPKRAFSQRKFFNSGEE---LFLFLFEVIVDYLADILENISKKIKQLSREILEEKQFSEEDLDAL 149

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 187 FELRKNLVRLRRVVLPMREVLNTLLRRDDLvgcSPAMQPFLQDVYDHVLRATEWTESLRDLVTTILET---HISIQGNQm 263
Cdd:cd12825   150 TKLNENNITLRESLIEKQRIITLLLKSPRE---NSDTKEKLKIELRDLSSLLDHTTFNFDKLDDLKNNilgKIELEQNK- 225

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1086933017 264 nlVMKKVTSWAAIIAVPTAVTGFFG---QNVPFFGFQsdYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:cd12825   226 --IIKIFTVVTVCFLPPTLIASIYGmnfKNMPELKWS--YGYPFALILMILSTILPLLYFKRKGWL 287
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 159-323 3.27e-06

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 48.01  E-value: 3.27e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 159 QELDGYIDALEESLfddhspDHDLQRKTFELRKNLVRLRRVVLPMREVLNTLLRRDD------LVGCSPAMQPFLQDVYD 232
Cdd:cd12823   135 PEALPLLDELTDKI------STSNLERLLPLKRRLVELETRVQKVRDALEELLDDDEdmadmyLTDKAAGPERLESSRKE 208

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 233 HVLRATEW--------TESLRDLVTTILET------HISIQ-GNQMNLVMK---KVTSWAAIIAVPTAVTGFFGQNVPFF 294
Cdd:cd12823   209 DDHEEVEMlleaylqqVDELLNKLEELREYiddteeLINLIlDSRRNQLMRlelKLSIGTLSLAVGTLVAGLFGMNLPSG 288

                         170       180
                  ....*....|....*....|....*....
gi 1086933017 295 GFQSDYGLWLSTALMVTGSVFLYLGFKRR 323
Cdd:cd12823   289 LEESPYAFWIVTGGSVVGSILIFIVVLRY 317
ZntB-like cd12824
Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging ...
 98-323 1.87e-05

Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213358 [Multi-domain]  Cd Length: 290  Bit Score: 45.59  E-value: 1.87e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017  98 DIAEVSAFVTRNALVTVH-GPGFDTDKLMAHwdaetdLAAHGVPFLFWGLLDMIVDGhfdAVQELDGYIDALEESL--FD 174
Cdd:cd12824    65 DMADLRILIGQGAAVTVRsGPSRAVDELRRA------LRDHSSLKSLADLLAQMISG---MTKRLELVIFDLTETLdeVE 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 175 DHSPDHDLQRKTF---ELRKNLVRLRRVVLPMREVLNTLLRRDDLVGCSPAMQPfLQDVYDHVLRATEWTESLRDLVtTI 251
Cdd:cd12824   136 DNLLDGGTAPPPYaliALQRRLFRIRRQIRPQQQALRPLITDPAELLVTSDRET-LRRASNNVTRYLETLDFCRTRV-AL 213

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1086933017 252 LETHISIQGNQMNLVMKKVTSWAAIIAVPTA-VTGFFGQNVPFF-GFQSDYGLWLSTALMVTGSVFLYLGFKRR 323
Cdd:cd12824   214 LQDEIKAQRDEKTTRSSLLLTVVATVFLPLTfITGLLGMNVGGIpDVESPMGFWWVTGLSILVALLAWLLLRRL 287
EcCorA_ZntB-like_u1 cd12826
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 105-326 1.12e-04

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA-ZntB_like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB_like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213360  Cd Length: 281  Bit Score: 42.94  E-value: 1.12e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 105 FVTRNALVTVHGPGFDTD--KLMAHWDAETdlaAHGVPFLFWGLLDMIVDGHFDAVQELDGYIDALEESLFDDHspDHDL 182
Cdd:cd12826    63 YITKNRLVFIDDDGFVLKllERIQEKKSWK---GPSLERFLYDFLEYLIKDDLEYLEKYEEKLEELEEEILDGK--LKNF 137

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 183 QRKTFELRKNLVRLRRV---VLPMREVL----NTLLRRDDLVGcspamqpfLQDVYDHVLRATEWTESLRDLVTTILETH 255
Cdd:cd12826   138 NERILKIRKELLRLNSYyeqLMDIGEELeeneNDLFDEEELRL--------FRLFSDRVERLHDNVQMLREYSLQIRELY 209

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1086933017 256 ISIQGNQMNLVMK---KVTSwaaiIAVP-TAVTGFFGQNVPFF-GFQSDYG---LWLSTALMVTGSVFLylgFKRRDWI 326
Cdd:cd12826   210 QSQIDIRQNRIMKiltVVTT----IFLPlTLITGWYGMNFTNMpELHWKYGypvVIILSILIVVIEIIY---FKKKKWL 281
zntB PRK09546
zinc transporter ZntB;
148-326 5.91e-03

zinc transporter ZntB;


Pssm-ID: 181941  Cd Length: 324  Bit Score: 38.06  E-value: 5.91e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 148 DMIVDGHFDAVQELDGYIDALEESLFDDHSPDhdlQRKTFELRKNLVRLRRVVLPMREVLNTL-------LRRDDLVGcs 220
Cdd:PRK09546  149 DALTDHASEFIEELHDKIIDLEDNLLDQQIPP---RGELALLRKQLIVMRRYMAPQRDVFARLaserlpwMSDDDRRR-- 223

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1086933017 221 pamqpfLQDVYDhvlRATEWTESLRDLV--TTILETHISiqgNQMNLVMKKVT---SWAAIIAVPTA-VTGFFGQN---V 291
Cdd:PRK09546  224 ------MQDIAD---RLGRGLDDLDACIarTAVLADEIA---SVMAEAMNRRTytmSLMAMVFLPTTfLTGLFGVNlggI 291

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 1086933017 292 PffGFQSDYGLWLSTALMVTGSVFLYLGFKRRDWI 326
Cdd:PRK09546  292 P--GGGWPFGFSIFCLLLVVLIGGVAWWLKRSKWL 324
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH