NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1732943895|gb|TYS20717|]
View 

nitroreductase family protein [Bacillus subtilis]

Protein Classification

nitroreductase family protein( domain architecture ID 10114825)

nitroreductase family protein may catalyze the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles, requiring NAD(P)H as an electron donor in an obligatory two-electron transfer and using FMN as cofactor

CATH:  3.40.109.10
EC:  1.7.1.-
Gene Ontology:  GO:0016491
PubMed:  25702712|7601851|29078300

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 5-202 7.18e-62

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


:

Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 189.37  E-value: 7.18e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   5 SQLVNERRSASNLLPDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDK 84
Cdd:cd02137     1 LEVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYNQPQVTTASAVILVLGDL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  85 qayqqaadiyeglkmlgilnkqeydhmvqdtvsfyenrgepfkrdeairNASLSAMMFMLSAKEKGWDTCPMIGFDAEAV 164
Cdd:cd02137    81 -------------------------------------------------NAGLAAMNLMLAAKAKGYDTCPMGGFDKEKV 111

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1732943895 165 KQILNIDDQFEIVMMITIGKEKTEsrRPRGYRKPVNEF 202
Cdd:cd02137   112 AELLNLPDRYVPVLLIAIGKAADK--APRSGRLPVDEV 147
 
Name Accession Description Interval E-value
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 5-202 7.18e-62

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 189.37  E-value: 7.18e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   5 SQLVNERRSASNLLPDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDK 84
Cdd:cd02137     1 LEVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYNQPQVTTASAVILVLGDL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  85 qayqqaadiyeglkmlgilnkqeydhmvqdtvsfyenrgepfkrdeairNASLSAMMFMLSAKEKGWDTCPMIGFDAEAV 164
Cdd:cd02137    81 -------------------------------------------------NAGLAAMNLMLAAKAKGYDTCPMGGFDKEKV 111

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1732943895 165 KQILNIDDQFEIVMMITIGKEKTEsrRPRGYRKPVNEF 202
Cdd:cd02137   112 AELLNLPDRYVPVLLIAIGKAADK--APRSGRLPVDEV 147
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-203 3.47e-42

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 139.60  E-value: 3.47e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   4 FSQLVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAAN--GQYKVLSSSAVLLVL 81
Cdd:COG0778     1 LLELLLTRRSVRKFTDK-PVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAeaNQEWVADAPVLIVVC 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  82 GDKqayqqaadiyeglkmlgilnkqeydhmvqdtvsfyeNRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDA 161
Cdd:COG0778    80 ADP------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIGGFDP 123

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1732943895 162 EAVKQILNIDDQFEIVMMITIGKEKtESRRPRGyRKPVNEFV 203
Cdd:COG0778   124 EKVRELLGLPEGEEPVALLALGYPA-EELNPRP-RKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 8-184 5.21e-31

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 111.33  E-value: 5.21e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   8 VNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDKQAY 87
Cdd:pfam00881   1 IRQRRSVRKFDPE-PVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRDANL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  88 QQAAD--IYEGLKMLgilnkqeydhMVQDTVSFYENRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVK 165
Cdd:pfam00881  80 KLLLQdfLRGAPVLI----------VITASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170
                  ....*....|....*....
gi 1732943895 166 QILNIDDQFEIVMMITIGK 184
Cdd:pfam00881 150 ELLGLPDDERLVGLIAVGY 168
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 6-205 5.87e-17

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 75.23  E-value: 5.87e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   6 QLVNERRSASNLLpDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANG--QYKVLSSSAVLLVlgd 83
Cdd:PRK05365   11 QLFTEARTHNGWL-DEPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKERLRPALSEgnLAKTLAAPVTAIV--- 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  84 kqayqqAADIyeglkmlgilnkQEYDHMVQ-----DTVSFYEnrGEPFKRDE-AIRNASLSAMMFMLSAKEKGWDTCPMI 157
Cdd:PRK05365   87 ------AYDT------------EFHEHLPKlfphaDARSWFA--GNPALAEEtAFRNSSLQGAYLILAARALGLDAGPMS 146

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1732943895 158 GFDAEAVKQILNIDDQFEIVMMITIGKEKTESRRPRGYRKPVNEFVEY 205
Cdd:PRK05365  147 GFDAAAVDAEFFAGTTWKSNFLVNIGYGDPAKLFPRLPRLSFDEACRI 194
 
Name Accession Description Interval E-value
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 5-202 7.18e-62

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 189.37  E-value: 7.18e-62
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   5 SQLVNERRSASNLLPDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDK 84
Cdd:cd02137     1 LEVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYNQPQVTTASAVILVLGDL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  85 qayqqaadiyeglkmlgilnkqeydhmvqdtvsfyenrgepfkrdeairNASLSAMMFMLSAKEKGWDTCPMIGFDAEAV 164
Cdd:cd02137    81 -------------------------------------------------NAGLAAMNLMLAAKAKGYDTCPMGGFDKEKV 111

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1732943895 165 KQILNIDDQFEIVMMITIGKEKTEsrRPRGYRKPVNEF 202
Cdd:cd02137   112 AELLNLPDRYVPVLLIAIGKAADK--APRSGRLPVDEV 147
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 4-203 3.47e-42

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 139.60  E-value: 3.47e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   4 FSQLVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAAN--GQYKVLSSSAVLLVL 81
Cdd:COG0778     1 LLELLLTRRSVRKFTDK-PVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEALAeaNQEWVADAPVLIVVC 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  82 GDKqayqqaadiyeglkmlgilnkqeydhmvqdtvsfyeNRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDA 161
Cdd:COG0778    80 ADP------------------------------------DRSEKVPERYALLDAGIAAQNLLLAARALGLGTCWIGGFDP 123

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1732943895 162 EAVKQILNIDDQFEIVMMITIGKEKtESRRPRGyRKPVNEFV 203
Cdd:COG0778   124 EKVRELLGLPEGEEPVALLALGYPA-EELNPRP-RKPLEEVV 163
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 8-184 5.21e-31

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 111.33  E-value: 5.21e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   8 VNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDKQAY 87
Cdd:pfam00881   1 IRQRRSVRKFDPE-PVPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAAALLLLLRRDANL 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  88 QQAAD--IYEGLKMLgilnkqeydhMVQDTVSFYENRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVK 165
Cdd:pfam00881  80 KLLLQdfLRGAPVLI----------VITASLSTYLRKAAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAAAVR 149

                         170
                  ....*....|....*....
gi 1732943895 166 QILNIDDQFEIVMMITIGK 184
Cdd:pfam00881 150 ELLGLPDDERLVGLIAVGY 168
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 11-190 1.95e-30

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 110.49  E-value: 1.95e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  11 RRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGD-KQAYQQ 89
Cdd:cd03370     8 RRSIRKYTQE-PVPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYGQAQVTSAPAVIVIYSDmEDALAN 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  90 AAD-IYEGlkmlgiLNKQEYDHMVQDTVSFYENRGEPfKRDEAIRNASLSAMMF-MLSAKEKGWDTCPMIGFDAEAVKQI 167
Cdd:cd03370    87 LEEtIHPG------LSEERRQREAAGLRGAFGKMSVE-QRGQWGLAQANIALGFlLLAAQSLGYDTSPMLGFDPEKVKAL 159

                         170       180
                  ....*....|....*....|...
gi 1732943895 168 LNIDDQFEIVMMITIGKEKTESR 190
Cdd:cd03370   160 LGLPEHVTIAALVALGKPAEEGY 182
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 4-205 8.50e-25

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 95.23  E-value: 8.50e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   4 FSQLVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGD 83
Cdd:cd02139     1 VYEAIKKRRSIRKYKPT-PVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANGQKFIAEAPVVIVACAD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  84 KQAYqqaadiyeglkmlGILNKQEYdHMVqdtvsfyenrgepfkrDEAIrnaslsAMMFM-LSAKEKGWDTCPMIGFDAE 162
Cdd:cd02139    80 PSES-------------GMGCGKPY-YLV----------------DVAI------AMEHLvLAATEEGLGTCWIGAFDED 123

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1732943895 163 AVKQILNIDDQFEIVMMITIGKEKtESRRPRGyRKPVNEFVEY 205
Cdd:cd02139   124 KVKEILGIPEEYRVVALTPLGYPA-EEPPPRP-RKPLEEIVFY 164
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 3-202 2.86e-21

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 85.77  E-value: 2.86e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   3 EFSQLVNERRSASNLLPDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANG-QYKVLSSSAVLLVL 81
Cdd:cd02149     1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFnQPQIKDASHVVVFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  82 GD-----KQAYQQAADIYEGLKMLGIlnkqeydhmvqdtvsfyenrgepfkrdeairnaslsammfmlsakekgwDTCPM 156
Cdd:cd02149    81 AKkdwsaKQTYIALGNMLLAAAMLGI-------------------------------------------------DSCPI 111

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1732943895 157 IGFDAEAVKQILNIDDQ-FEIVMMITIGKEKTESrRPRgYRKPVNEF 202
Cdd:cd02149   112 EGFDPAKLDEILGLDEKgYKISVMVAFGYRSEEK-LPK-SRKPLEDV 156
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 7-203 1.19e-20

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 84.52  E-value: 1.19e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   7 LVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPD-VKEKLKQAAN--GQYKVLSSSAVLLVLGD 83
Cdd:cd02138     1 LIAERWSPRAFSPE-PISEEDLLSLFEAARWAPSCFNEQPWRFVVARRDTeAFEKLLDLLAegNQSWAKNAPVLIVVLAK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  84 KqayqqaadiyeglkmlgilnkqEYDHmvqdtvsfyenRGEPfkRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEA 163
Cdd:cd02138    80 T----------------------EFDH-----------NGKP--NRYALFDTGAAVANLALQATALGLVVHQMAGFDPEK 124

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1732943895 164 VKQILNIDDQFEIVMMITIGK------------EKTESRRPrgyRKPVNEFV 203
Cdd:cd02138   125 AKEALGIPDEYEPITMIAIGYpgdpeslpekllEREEAPRT---RKPLSEIV 173
RutE-like cd02148
nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family ...
 6-196 3.25e-19

nitroreductase similar to Escherichia coli RutE; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. RutE is involved in the utilization of uracil as the sole nitrogen source; it appears to have the same function as YdfG, which reduces malonic semialdehyde to 3-hydroxypropionic acid.


Pssm-ID: 380323 [Multi-domain]  Cd Length: 186  Bit Score: 81.15  E-value: 3.25e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   6 QLVNERRSASNLLpDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANG--QYKVLSSSAVLLVLGD 83
Cdd:cd02148     4 QLFTEARTHNAWE-DKPVSDEELRAIYDLAKWGPTAANCSPARIVFVRSAEAKERLVPHLSEgnREKTMAAPVTAILAYD 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  84 KQAYQQaadiyeglkmlgiLNKQeYDHMvqDTVSFYENRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEA 163
Cdd:cd02148    83 TEFYEH-------------LPRL-FPHG--DARSWFFGSGPARAEETAFRNASLQAAYFILAARALGLDCGPMSGFDAAG 146

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1732943895 164 VKQILNIDDQFEIVMMITIGKEKTESRRPRGYR 196
Cdd:cd02148   147 VDAEFFAGTRWKSNLVVNLGYGDPSKLFPRLPR 179
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 11-183 5.01e-19

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 79.26  E-value: 5.01e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  11 RRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLgdkqayqqa 90
Cdd:cd02062     4 RRSIRKFTDK-PVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAAPNQKFIAGAPVVIVV--------- 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  91 adiyeglkmlgILNKQEYDHMvqdtvsfyenrgepfkrdeAIRNASLSAMMFMLSAKEKGWDTCPMIGFD--AEAVKQIL 168
Cdd:cd02062    74 -----------VADPDKSRPW-------------------ALEDAGAAAQNLLLAAAALGLGSCWIGGFDfrEDKVRELL 123

                         170
                  ....*....|....*
gi 1732943895 169 NIDDQFEIVMMITIG 183
Cdd:cd02062   124 GIPENLRPVALIAIG 138
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 3-183 1.71e-18

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 78.20  E-value: 1.71e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   3 EFSQLVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVlssSAVLLVLG 82
Cdd:cd20609     1 DFLELAKKRYSVRKFSDK-PVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAKATPRFFGA---PLVIVVCY 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  83 DK-QAYQQAADiyeglkmlgilnkqEYDHMVQDtvsfyenrgepfkrdeairnASLSAMMFMLSAKEKGWDTCPMIGFDA 161
Cdd:cd20609    77 DKdESWKRPYD--------------GKDSGDID--------------------AAIVATHMMLAATELGLGTCWVGNFDP 122

                         170       180
                  ....*....|....*....|..
gi 1732943895 162 EAVKQILNIDDQFEIVMMITIG 183
Cdd:cd20609   123 EKVREAFNLPENLEPVAILPLG 144
PRK05365 PRK05365
malonic semialdehyde reductase; Provisional
 6-205 5.87e-17

malonic semialdehyde reductase; Provisional


Pssm-ID: 180040 [Multi-domain]  Cd Length: 195  Bit Score: 75.23  E-value: 5.87e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   6 QLVNERRSASNLLpDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANG--QYKVLSSSAVLLVlgd 83
Cdd:PRK05365   11 QLFTEARTHNGWL-DEPVSDEQLRELYDLVKWGPTSANCSPARFVFVRSAEAKERLRPALSEgnLAKTLAAPVTAIV--- 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  84 kqayqqAADIyeglkmlgilnkQEYDHMVQ-----DTVSFYEnrGEPFKRDE-AIRNASLSAMMFMLSAKEKGWDTCPMI 157
Cdd:PRK05365   87 ------AYDT------------EFHEHLPKlfphaDARSWFA--GNPALAEEtAFRNSSLQGAYLILAARALGLDAGPMS 146

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*...
gi 1732943895 158 GFDAEAVKQILNIDDQFEIVMMITIGKEKTESRRPRGYRKPVNEFVEY 205
Cdd:PRK05365  147 GFDAAAVDAEFFAGTTWKSNFLVNIGYGDPAKLFPRLPRLSFDEACRI 194
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 8-183 9.07e-12

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 60.75  E-value: 9.07e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   8 VNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDpdvKEKLKQAANgqykvlsssavllvlgdkqAY 87
Cdd:cd20610     1 IKKRRSIRKFKPD-PVPKEDIEKILEAANWAPSGMNRQNWEFVVVKG---GEKIEKIGI-------------------SI 57

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  88 QQAADIYEGLKMLGILNKQeydHMVQDTVSFYENRG----------EPFKRDEaIRNA---SLSAMM--FMLSAKEKGWD 152
Cdd:cd20610    58 KKKNEEIARLLEKVFAEKP---IRFRKFRRFFTLFGgapvlvvvytEPYKPPE-ERKPdlqSVSAAIqnLLLAAHALGLG 133

                         170       180       190
                  ....*....|....*....|....*....|...
gi 1732943895 153 TCPMIG--FDAEAVKQILNIDDQFEIVMMITIG 183
Cdd:cd20610   134 TCWMTGplYAEDEIEEILEIPDDKELVAVTPLG 166
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 8-183 1.07e-10

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 57.34  E-value: 1.07e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   8 VNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLgdkqay 87
Cdd:cd20608     4 IKTRRSVRRFSDK-PVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPSNGWLKDAPVIIVV------ 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  88 qqAADIYEGlkmlGILNKQEYdHMVqdtvsfyenrgepfkrdeairNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKQI 167
Cdd:cd20608    77 --CADPKDS----GWLNGQNY-YLV---------------------DAAIAMQNLMLAATDLGLGTCWIGAFDEKKVKEI 128

                         170
                  ....*....|....*.
gi 1732943895 168 LNIDDQFEIVMMITIG 183
Cdd:cd20608   129 LGIPENIRVVALTPLG 144
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 6-193 3.38e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 53.69  E-value: 3.38e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   6 QLVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLS-SSAVLLVLGDK 84
Cdd:cd02151     1 ELLKKRRSIRKYTDE-PIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECKPHGSAFLKgAPAAIVVLADT 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  85 qayqQAADIYeglkmlgilnkqeydhmVQDtvsfyenrgepfkrdeairnASLSAMMFMLSAKEKGWDTC-------PMI 157
Cdd:cd02151    80 ----EKSDTW-----------------IED--------------------ASIAATYIQLAAESLGLGSCwiqirnrETQ 118

                         170       180       190
                  ....*....|....*....|....*....|....*...
gi 1732943895 158 -GFDAE-AVKQILNIDDQFEIVMMITIGKeKTESRRPR 193
Cdd:cd02151   119 dGKTAEeYVRELLGIPENYRVLCIIALGY-PDEEKPPH 155
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 7-203 1.36e-08

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 51.82  E-value: 1.36e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   7 LVNERRSASNLLPDhPITKDELNEMFELVALAPSAFNLQHTKyVTVLDPDVKEKLKQAANGqykvlsSSAVLLVLGDKQA 86
Cdd:cd02136     1 AIKSRRSVRAFKDK-PVPKETIEKILEAARRAPSGKNTQPWR-VYVVTGKARERLKKAFFG------APVALFLTMDKVL 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  87 YQQAAdiyeglkmlgilnkqeYDhmvqdtvsfyenrgepfkrdeairnASLSAMMFMLSAKEKGWDTCPmIGF---DAEA 163
Cdd:cd02136    73 GPWSW----------------FD-------------------------LGAFLQNLMLAAHALGLGTCP-QGAlagYPDV 110

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1732943895 164 VKQILNIDDQFEIV--MMITIGKEKTESRRPRGYRKPVNEFV 203
Cdd:cd02136   111 VRKELGIPDDEELVcgIALGYPDPDAPVNQFRTPREPLEEFV 152
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
3-183 4.39e-07

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 48.43  E-value: 4.39e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895   3 EFSQLVNERRSASNLLPDHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQY-----KVLSSSAV 77
Cdd:PRK11053    2 DIVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKAAAGNYafnerKILDASHV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1732943895  78 LlVLGDK----QAY------QQAADiyeglkmlGILNKQEYDHMVQDTVSFYEN--RGEPfkRDEAI---RNASLSAMMF 142
Cdd:PRK11053   82 V-VFCAKtdmdDAYlelvleQEDAD--------GRFATEEAKAAQDKGRRFFADmhRKEL--KDLQHwmeKQVYLALGNL 150

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 1732943895 143 MLSAKEKGWDTCPMIGFDAEAVKQILNIDDQ-FEIVMMITIG 183
Cdd:PRK11053  151 LLGAAALGIDATPIEGFDAAILDAEFGLREKgLTSSVVVPLG 192
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 20-92 3.26e-05

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 43.38  E-value: 3.26e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1732943895  20 DHPITKDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEKLKQAANGQYKVLSSSAVLLVLGDKQAYQQAAD 92
Cdd:cd02146    16 DEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELAGNQPYVAQAPVFLVFCADLYRHQKIAE 88
McbC_SagB-like_oxidoreductase cd02142
oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain ...
 141-184 4.02e-05

oxidase similar to the microcin B17 processing protein McbC; This family is the oxidase domain of NRPS (non-ribosomal peptide synthetase) and other systems that modify polypeptides by cyclizing a thioester to form a ring. These include EpoB, part of the epothilone biosynthesis pathway; TubD, part of the tubulysin biosynthesis pathway, MtsD, part of the myxothiozol biosynthesis pathway; IndC, part of the indigoidine biosynthesis pathway and TfxB, part of the trifitoxin processing pathway. All are FMN-dependent and oxidize the product of the cyclization of thioesters in short polypeptides.


Pssm-ID: 380318  Cd Length: 200  Bit Score: 42.79  E-value: 4.02e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 1732943895 141 MFMLSAKEKGWDTCPMIGFDAEAVKQILNIDDQFEIVM-MITIGK 184
Cdd:cd02142   156 NLYLAATALGLGLCAIGAFDDDALRELLGLDEVEEVVLyAFVVGG 200
Frm2-like cd02140
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 4-53 8.88e-03

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to nitroreductase. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer. Members of this family are also called NADH dehydrogenase, oxygen-insensitive NAD(P)H nitrogenase or dihydropteridine reductase.


Pssm-ID: 380317  Cd Length: 192  Bit Score: 35.74  E-value: 8.88e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1732943895   4 FSQLVNERRSASNLLPDHPITKDELNEMFELVAL-APSAFNLQHTKYVTVL 53
Cdd:cd02140     1 FLEAAKARRTIYALGKNSPVSDEEIEEIVKEAVKhVPSSFNSQSSRAVILL 51
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH