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Conserved domains on  [gi|2295394060|gb|UWI20203|]
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MAG: glycosyl hydrolase family 25 [Bacteriophage sp.]

Protein Classification

glycoside hydrolase family 25 domain-containing protein( domain architecture ID 2550)

glycoside hydrolase family 25 domain-containing protein is a peptidoglycan hydrolase (also called lysozyme) that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GH25_muramidase super family cl10448
Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan ...
 3-207 4.04e-67

Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.


The actual alignment was detected with superfamily member cd06417:

Pssm-ID: 447900  Cd Length: 195  Bit Score: 206.14  E-value: 4.04e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   3 LNGIDISHHQGEAGMTVsifekMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTNAEKEVDYFLTHCK 82
Cdd:cd06417     1 MNGIDVSSWQSRIVTTV-----VPADFVIVKATQGTGYVNPSWRSQAAQAIAAGKLLGLYHYANGGNAIAEADYFLNNIK 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  83 NYVGEALIALDWEKSYNQSFDNPNWAKQWLDIFYEKTGIRPLIYMSDSVTEAHNWsDVAKNYGLWIAQYPDDSIVhGYQS 162
Cdd:cd06417    76 GYVGKAVLVLDWESYQNSAWGNSAWARQWVNRVHELTGVWPMVYVSKSVTRQINW-SVRADCGLWVAQYASNNPT-GYQS 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2295394060 163 NpwrDGDGQGAFAITAIHQYTASGRISPWTGNLDLDIAYMSKEAW 207
Cdd:cd06417   154 Q---AGPWNAAWSGETIHQYTSNGSLNGYNGPLDLNLFYGDREQW 195
 
Name Accession Description Interval E-value
GH25_LysA-like cd06417
LysA is a cell wall endolysin produced by Lactobacillus fermentum, which degrades bacterial ...
 3-207 4.04e-67

LysA is a cell wall endolysin produced by Lactobacillus fermentum, which degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. The N-terminal glycosyl hydrolase family 25 (GH25) domain of LysA has sequence similarity with other murein hydrolase catalytic domains while the C-terminal domain has sequence similarity with putative bacterial cell wall-binding SH3b domains. This domain family also includes LysL of Lactococcus lactis.


Pssm-ID: 119379  Cd Length: 195  Bit Score: 206.14  E-value: 4.04e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   3 LNGIDISHHQGEAGMTVsifekMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTNAEKEVDYFLTHCK 82
Cdd:cd06417     1 MNGIDVSSWQSRIVTTV-----VPADFVIVKATQGTGYVNPSWRSQAAQAIAAGKLLGLYHYANGGNAIAEADYFLNNIK 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  83 NYVGEALIALDWEKSYNQSFDNPNWAKQWLDIFYEKTGIRPLIYMSDSVTEAHNWsDVAKNYGLWIAQYPDDSIVhGYQS 162
Cdd:cd06417    76 GYVGKAVLVLDWESYQNSAWGNSAWARQWVNRVHELTGVWPMVYVSKSVTRQINW-SVRADCGLWVAQYASNNPT-GYQS 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2295394060 163 NpwrDGDGQGAFAITAIHQYTASGRISPWTGNLDLDIAYMSKEAW 207
Cdd:cd06417   154 Q---AGPWNAAWSGETIHQYTSNGSLNGYNGPLDLNLFYGDREQW 195
Acm COG3757
Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis] ...
 1-211 1.54e-43

Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442971  Cd Length: 208  Bit Score: 146.20  E-value: 1.54e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   1 MSLNGIDISHHQGEagMTVSIFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA-NGTNAEKEVDYFLT 79
Cdd:COG3757     9 YPVHGIDVSHYQGD--IDWAAVKAAGIDFAYIKATEGTDYVDPKFARNWAGARAAGLPRGAYHFFrPCSDAAAQADNFIS 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCKNYVGEALIALDWEKSYNQSFDNPN---WAKQWLDIFYEKTGIRPLIYMSDSVTEAH-NWSDVAkNYGLWIAQYPDDs 155
Cdd:COG3757    87 TVPRDPGDLPPVLDLEENGYYGLSPAQlraWLKAFLDEVEAHTGRKPIIYTSPSFYNDYlGNSDFS-DYPLWIARYGSS- 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2295394060 156 iVHGYQSNPWrdgdgqgafaitAIHQYTASGRISPWTGNLDLDIAYMSKEAWGKYA 211
Cdd:COG3757   165 -PGYLPGRNW------------TFWQYTSSGRVPGISGNVDLNVFNGSRDELKALA 207
Glyco_hydro_25 pfam01183
Glycosyl hydrolases family 25;
6-189 8.39e-28

Glycosyl hydrolases family 25;


Pssm-ID: 426105  Cd Length: 180  Bit Score: 104.75  E-value: 8.39e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   6 IDISHHQGEAGmtvsiFEKMSFD---FAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA---NGTNAEKEVDYFLT 79
Cdd:pfam01183   1 IDVSSYQGDID-----WQKVKASgvsFVFIKATEGTDYVDPYFTTQYANARAAGLKVGAYHFArpcNSSTAAAQADYFLS 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCKNYVGEA---LIALDWEKSYNQS-FDNPNWAKQWLDIFYEKTGIRPLIYMSDSV-TEAHNWSDVAKNYGLWIAQYPdd 154
Cdd:pfam01183  76 NVQGLGLDAgtlPPVLDVEVTTGLTkAAATSNILRFLDRVKKQTGYKPVIYTGTSFwTNNLLYGQFIADYPLWIASYA-- 153

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 2295394060 155 siVHGYQSNPWRDGdgqgafaiTAIHQYTASGRIS 189
Cdd:pfam01183 154 --VTPPKDYPGWTK--------WTFWQYTSSGSIP 178
Glyco_25 smart00641
Glycosyl hydrolases family 25;
3-95 4.44e-08

Glycosyl hydrolases family 25;


Pssm-ID: 128889  Cd Length: 109  Bit Score: 50.11  E-value: 4.44e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060    3 LNGIDISHHQGEAGMtvSIFEKMSFDFAIMKATDGTRFVDA-WCDKYYQSAKKRGKLLGVYHYA--NGTNAEKEVDYFLT 79
Cdd:smart00641   1 TKGIDVSDYEGGIDG--AKVRNTGASFAFMKATEGAGYTPPyYSYQYFLADNAGYILRGFYHAAypVSSSATAQANYFPS 78

                           90       100
                   ....*....|....*....|.
gi 2295394060   80 HCKNYVGE-----ALIALDWE 95
Cdd:smart00641  79 MDGVRIFQftsnpFVLDLDGN 99
 
Name Accession Description Interval E-value
GH25_LysA-like cd06417
LysA is a cell wall endolysin produced by Lactobacillus fermentum, which degrades bacterial ...
 3-207 4.04e-67

LysA is a cell wall endolysin produced by Lactobacillus fermentum, which degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. The N-terminal glycosyl hydrolase family 25 (GH25) domain of LysA has sequence similarity with other murein hydrolase catalytic domains while the C-terminal domain has sequence similarity with putative bacterial cell wall-binding SH3b domains. This domain family also includes LysL of Lactococcus lactis.


Pssm-ID: 119379  Cd Length: 195  Bit Score: 206.14  E-value: 4.04e-67
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   3 LNGIDISHHQGEAGMTVsifekMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTNAEKEVDYFLTHCK 82
Cdd:cd06417     1 MNGIDVSSWQSRIVTTV-----VPADFVIVKATQGTGYVNPSWRSQAAQAIAAGKLLGLYHYANGGNAIAEADYFLNNIK 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  83 NYVGEALIALDWEKSYNQSFDNPNWAKQWLDIFYEKTGIRPLIYMSDSVTEAHNWsDVAKNYGLWIAQYPDDSIVhGYQS 162
Cdd:cd06417    76 GYVGKAVLVLDWESYQNSAWGNSAWARQWVNRVHELTGVWPMVYVSKSVTRQINW-SVRADCGLWVAQYASNNPT-GYQS 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2295394060 163 NpwrDGDGQGAFAITAIHQYTASGRISPWTGNLDLDIAYMSKEAW 207
Cdd:cd06417   154 Q---AGPWNAAWSGETIHQYTSNGSLNGYNGPLDLNLFYGDREQW 195
Acm COG3757
Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis] ...
 1-211 1.54e-43

Lyzozyme M1 (1,4-beta-N-acetylmuramidase), GH25 family [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442971  Cd Length: 208  Bit Score: 146.20  E-value: 1.54e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   1 MSLNGIDISHHQGEagMTVSIFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA-NGTNAEKEVDYFLT 79
Cdd:COG3757     9 YPVHGIDVSHYQGD--IDWAAVKAAGIDFAYIKATEGTDYVDPKFARNWAGARAAGLPRGAYHFFrPCSDAAAQADNFIS 86

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCKNYVGEALIALDWEKSYNQSFDNPN---WAKQWLDIFYEKTGIRPLIYMSDSVTEAH-NWSDVAkNYGLWIAQYPDDs 155
Cdd:COG3757    87 TVPRDPGDLPPVLDLEENGYYGLSPAQlraWLKAFLDEVEAHTGRKPIIYTSPSFYNDYlGNSDFS-DYPLWIARYGSS- 164

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2295394060 156 iVHGYQSNPWrdgdgqgafaitAIHQYTASGRISPWTGNLDLDIAYMSKEAWGKYA 211
Cdd:COG3757   165 -PGYLPGRNW------------TFWQYTSSGRVPGISGNVDLNVFNGSRDELKALA 207
GH25_muramidase cd00599
Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan ...
 5-200 1.69e-35

Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.


Pssm-ID: 119373 [Multi-domain]  Cd Length: 186  Bit Score: 124.77  E-value: 1.69e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   5 GIDISHHQGEAGMTVsiFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA-NGTNAEKEVDYFLTHCKN 83
Cdd:cd00599     2 GIDVSSWQGSIDWNA--VKAAGIDFVFIKATEGTTYVDPKFATNRARARAAGLLVGAYHFArPCANAEAQADNFVNTVPR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  84 YVGEALIALDWE--KSYNQSFDNPNWAKQWLDIFYEKTGIRPLIYMSDSVTEAHNWSDVAKNYGLWIAQYPDDSIVHGYQ 161
Cdd:cd00599    80 DPGSLPLVLDVEdtGGGCSAAALAAWLNAFLNEVEALTGKKPIIYTSPSFWDDYLASSQLSDYPLWIAHYRGEPPPAPGA 159

                         170       180       190
                  ....*....|....*....|....*....|....*....
gi 2295394060 162 SNPWRdgdgqgafaitaIHQYTASGRISPWTGNLDLDIA 200
Cdd:cd00599   160 WRPWT------------LWQYTSSGRVPGISGPVDLNVF 186
Glyco_hydro_25 pfam01183
Glycosyl hydrolases family 25;
6-189 8.39e-28

Glycosyl hydrolases family 25;


Pssm-ID: 426105  Cd Length: 180  Bit Score: 104.75  E-value: 8.39e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   6 IDISHHQGEAGmtvsiFEKMSFD---FAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA---NGTNAEKEVDYFLT 79
Cdd:pfam01183   1 IDVSSYQGDID-----WQKVKASgvsFVFIKATEGTDYVDPYFTTQYANARAAGLKVGAYHFArpcNSSTAAAQADYFLS 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCKNYVGEA---LIALDWEKSYNQS-FDNPNWAKQWLDIFYEKTGIRPLIYMSDSV-TEAHNWSDVAKNYGLWIAQYPdd 154
Cdd:pfam01183  76 NVQGLGLDAgtlPPVLDVEVTTGLTkAAATSNILRFLDRVKKQTGYKPVIYTGTSFwTNNLLYGQFIADYPLWIASYA-- 153

                         170       180       190
                  ....*....|....*....|....*....|....*
gi 2295394060 155 siVHGYQSNPWRDGdgqgafaiTAIHQYTASGRIS 189
Cdd:pfam01183 154 --VTPPKDYPGWTK--------WTFWQYTSSGSIP 178
GH25_Lyc-like cd06525
Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by ...
 4-198 1.25e-24

Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.


Pssm-ID: 119385  Cd Length: 184  Bit Score: 96.60  E-value: 1.25e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   4 NGIDISHHQG--------EAGMTVsifekmsfdfAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGT-NAEKEV 74
Cdd:cd06525     1 KGIDISNWQGninfnavkDSGVEV----------VYIKATEGTTFVDSYFNENYNGAKAAGLKVGFYHFLVGTsNPEEQA 70

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  75 DYFLTHCKNYVGEALIALDWEKSYNQSFDN-PNWAKQWLDIFYEKTGIRPLIYmSDSVTEAHNWSDVAKNYGLWIAQYPD 153
Cdd:cd06525    71 ENFYNTIKGKKMDLKPALDVEVNFGLSKDElNDYVLRFIEEFEKLSGLKVGIY-TYTSFINNNLDSRLSSYPLWIANYGV 149

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*
gi 2295394060 154 DSivhGYQSNPWRDGDGqgafaitaiHQYTASGRISPWTGNLDLD 198
Cdd:cd06525   150 SP---PSSNGIWNSWVG---------FQYSETGRVNGVSGSVDLD 182
GH25_YegX-like cd06524
YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) ...
 5-199 2.34e-20

YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.


Pssm-ID: 119384  Cd Length: 194  Bit Score: 85.47  E-value: 2.34e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   5 GIDISHHQGEAGMT-VSIFEKMS-FDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYH-YANGTNAEKEVDYFLTHC 81
Cdd:cd06524     2 GIDVSHYQGKIDWQkVKAKVKDSpVAFVFIKATEGVDIVDPDFPTNWEGAKEAGIIRGAYHfYRPNSDPKQQADNFLNTV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  82 KNYVGEALIA-LDWEKSYNQSFDNP--NWAKQWLDIFYEKTGIRPLIYMSDSVTEAHNWSDVAKNYGLWIAQYpddsivh 158
Cdd:cd06524    82 KLLGPGDLPPvLDVEWDGRKSSAKQiqEGVLEWLDAVEKATGVKPIIYTNPSFWTDYLTDSSFSEYPLWIADY------- 154

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|.
gi 2295394060 159 gYQSNPWRDGDGQGAfaiTAIHQYTASGRISPWTGNLDLDI 199
Cdd:cd06524   155 -NPRRKKVPPNESKK---WLLWQYSDSGKVPGISGAVDLNV 191
GH25_LytC-like cd06414
The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves ...
 5-201 4.28e-16

The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.


Pssm-ID: 119376  Cd Length: 191  Bit Score: 73.76  E-value: 4.28e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   5 GIDISHHQGEAGmtvsiFEKMS---FDFAIMKA---TDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTN---AEKEVD 75
Cdd:cd06414     3 GIDVSEWQGDID-----WKKVKasgVDFAIIRAgygGYGELQEDKYFEENIKGAKAAGIPVGVYFYSYAVTvaeAREEAE 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  76 YFLTHCKNYVGEALIALDWEksYNQSFDNPNWAKQWLDI---FY---EKTGIRPLIYMSdsvteaHNW------SDVAKN 143
Cdd:cd06414    78 FVLRLIKGYKLSYPVYYDLE--DETQLGAGLSKDQRTDIanaFCetiEAAGYYPGIYAN------LSWltnkldDERLSK 149

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 2295394060 144 YGLWIAQYpddsivhGYQSNPWRDGDGQgafaitaihQYTASGRISPWTGNLDLDIAY 201
Cdd:cd06414   150 YDVWVAQY-------GNSPTYPGNYGMW---------QYTSSGSVPGISGNVDLNYCY 191
GH25_Cpl1-like cd06415
Cpl-1 lysin (also known as Cpl-9 lysozyme / muramidase) is a bacterial cell wall endolysin ...
 4-197 1.01e-12

Cpl-1 lysin (also known as Cpl-9 lysozyme / muramidase) is a bacterial cell wall endolysin encoded by the pneumococcal bacteriophage Cp-1, which cleaves the glycosidic N-acetylmuramoyl-(beta1,4)-N-acetylglucosamine bonds of the pneumococcal glycan chain, thus acting as an enzymatic antimicrobial agent (an enzybiotic) against streptococcal infections. Cpl-1 belongs to the CP family of lysozymes (CPL lysozymes) which includes the Cpl-7 lysin. Cpl-1 has a glycosyl hydrolase family 25 (GH25) catalytic domain with an irregular (beta/alpha)5-beta3 barrel and a C-terminal cell wall-anchoring module formed by six similar choline-binding repeats (ChBr's). The ChBr's facilitate the anchoring of Cpl-1 to the choline-containing teichoic acid of the pneumococcal cell wall. Other members of this domain family have an N-terminal CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain similar to that of the firmicute CHAP lysins and associated with endopeptidase activity. The Cpl-7 lysin is also included here as is LysB of Lactococcus phage, and the Mur lysin of Lactobacillus phage.


Pssm-ID: 119377  Cd Length: 196  Bit Score: 64.73  E-value: 1.01e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   4 NGIDISHHQGEagmTVSIFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYAN-GTN---AEKEVDYFLT 79
Cdd:cd06415     2 YGVDVASYQGT---DLTAYGQAGAKFAIVKISEGTNYVNPKASAQVSSAIANGKMTGGYHFARfGGSvsqAKYEADYFLN 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCKNYVGEA--LIALDWEKSYNQSFDNPNWA-KQWLDIFYEkTGIRPLIYMSDSVTEAH-NWSDVAKNYG--LWIAQYPD 153
Cdd:cd06415    79 SAQQAGLPKgsYLALDYEQGSGNSKAANTSAiLAFMDTIKD-AGYKPMLYSYKPLLLNNvDYSQIIAKYPnsLWVAAYPT 157

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 2295394060 154 DSIVHG--YQSNPWRDGdgqgafaiTAIHQYTASGRISPWTGNLDL 197
Cdd:cd06415   158 YGVQDTpdFNYFPSMDG--------VAIWQFTSNWRGGGVDGNITL 195
GH25_CH-type cd06412
CH-type (Chalaropsis-type) lysozymes represent one of four functionally-defined classes of ...
 5-154 5.39e-12

CH-type (Chalaropsis-type) lysozymes represent one of four functionally-defined classes of peptidoglycan hydrolases (also referred to as endo-N-acetylmuramidases) that cleave bacterial cell wall peptidoglycans. CH-type lysozymes exhibit both lysozyme (acetylmuramidase) and diacetylmuramidase activity. The first member of this family to be described was a muramidase from the fungus Chalaropsis. However, a majority of the CH-type lysozymes are found in bacteriophages and Gram-positive bacteria such as Streptomyces and Clostridium. CH-type lysozymes have a single glycosyl hydrolase family 25 (GH25) domain with an unusual beta/alpha-barrel fold in which the last strand of the barrel is antiparallel to strands beta7 and beta1. Most CH-type lysozymes appear to lack the cell wall-binding domain found in other GH25 muramidases.


Pssm-ID: 119374  Cd Length: 199  Bit Score: 62.72  E-value: 5.39e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   5 GIDISHHQGEAGMTVSIFEKMSFdfAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYA--NGTNAEKEVDYFLTHCK 82
Cdd:cd06412     3 GIDVSGHQGSVDWSGAAANGARF--AYVKATEGTSYTNPRFSSQYNGAYNAGLIRGAYHFAlpDQSSGAAQADYFLDHGG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  83 NYVGEALI---ALDWE------KSYNQS-FDNPNWAKQWLDIFYEKTGIRPLIYMSDS--VTEAHNWSDVAkNYGLWIAQ 150
Cdd:cd06412    81 GWSPDGRTlpgVLDLEynpygaTCYGLSpAQMVSWIKDFSDTYKARTGRDPVIYTTTSwwNQCTGNSAGFA-NHPLWLAR 159


                  ....
gi 2295394060 151 YPDD 154
Cdd:cd06412   160 YGSS 163
GH25_muramidase_1 cd06413
Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) ...
 5-201 9.17e-12

Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.


Pssm-ID: 119375  Cd Length: 191  Bit Score: 61.91  E-value: 9.17e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   5 GIDISHHQGEAGMTVsiFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYH-YANGTNAEKEVDYFLTHCKN 83
Cdd:cd06413     5 GIDVSHHQGDIDWAR--VRAQGVSFAYIKATEGGDHVDKRFAENWRGARAAGLPRGAYHfFTFCRSGAEQAANFIRNVPK 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  84 YVGEALIALDWEKSYNqSFDNPNWAK--QWLDIFYEK----TGIRPLIYMSDSvTEAHNWSDVAKNYGLWIAQYpdDSIV 157
Cdd:cd06413    83 DPGALPPVVDVEWNGN-SATCPSAEEvlAELQVFLDAleahYGKRPIIYTTYD-FYDDYLKGEFPDYPLWIRSV--AGHP 158

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 2295394060 158 HGYQSNPWrdgdgqgafaitAIHQYTASGRISPWTGNLDLDIAY 201
Cdd:cd06413   159 RLYEDRPW------------TFWQYTNRGRVPGIEGDVDLNVFN 190
GH25_PlyB-like cd06523
PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus ...
 6-197 4.59e-10

PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.


Pssm-ID: 119383  Cd Length: 177  Bit Score: 56.99  E-value: 4.59e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   6 IDISHHQGEAGMTVSIFEKmSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTN---AEKEVDYFLT--- 79
Cdd:cd06523     3 VDISEWQGPINWDYDTLSK-QLDLVIIRVQYGSNYVDLKYKNNIKEFKKRGIPFGVYAFARGTStadAKAEARDFYNran 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 -HCKNYVgealiaLDWEKsynQSFDNPNWA-KQWLDIFYEKTGIRPLIYMSDSVTEAHNwSDVAKNYGLWIAQYPDDSIV 157
Cdd:cd06523    82 kKPTFYV------LDVEV---TSMSDMNAGvQAFISELRRLGAKKVGLYIGHHFYTTFN-LAVSKFDAIWIPAYGSNPGT 151

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 2295394060 158 HGYQsnpwrdgdgqgafaitaIHQYTASGRISPWTGNLDL 197
Cdd:cd06523   152 YPYD-----------------LWQYTDSGYLPGISGNVDL 174
Glyco_25 smart00641
Glycosyl hydrolases family 25;
3-95 4.44e-08

Glycosyl hydrolases family 25;


Pssm-ID: 128889  Cd Length: 109  Bit Score: 50.11  E-value: 4.44e-08
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060    3 LNGIDISHHQGEAGMtvSIFEKMSFDFAIMKATDGTRFVDA-WCDKYYQSAKKRGKLLGVYHYA--NGTNAEKEVDYFLT 79
Cdd:smart00641   1 TKGIDVSDYEGGIDG--AKVRNTGASFAFMKATEGAGYTPPyYSYQYFLADNAGYILRGFYHAAypVSSSATAQANYFPS 78

                           90       100
                   ....*....|....*....|.
gi 2295394060   80 HCKNYVGE-----ALIALDWE 95
Cdd:smart00641  79 MDGVRIFQftsnpFVLDLDGN 99
GH25_AtlA-like cd06522
AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell ...
 6-201 2.66e-06

AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family includes the AtlA and Aml autolysins from Streptococcus mutans which have a C-terminal glycosyl hydrolase family 25 (GH25) catalytic domain as well as six tandem N-terminal repeats of the GBS (group B Streptococcus) Bsp-like peptidoglycan-binding domain. Other members of this family have one or more C-terminal peptidoglycan-binding domain(s) (SH3 or LysM) in addition to the GH25 domain.


Pssm-ID: 119382  Cd Length: 192  Bit Score: 46.59  E-value: 2.66e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   6 IDISHHQGEagMTVSIFEKMS---FDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYHYANGTNAEK---EVDYFLT 79
Cdd:cd06522     4 VDVSSNNGI--MSVADYNKLKnygVKAVIVKLTEGTTYRNPYAASQIANAKAAGLKVSAYHYAHYTSAADaqaEARYFAN 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060  80 HCK--NYVGEALIALDWEKSyNQSFDNPNWAKQWLD----IFYEKTGirplIYMSDS-VTEAHNWSDVAKNyGLWIAQYP 152
Cdd:cd06522    82 TAKslGLSKNTVMVADMEDS-SSSGNATANVNAFWQtmkaAGYKNTD----VYTSASwLNSRADTSTLGAK-RVWVAQYP 155

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2295394060 153 ddsivhgYQ---SNPWRDGDGQgafaitaiHQYTASGRISPWTGNLDLDIAY 201
Cdd:cd06522   156 -------YNpssNNLWNTNYGA--------WQWTSQAHFPGRSGGFDVSIDY 192
GH25_muramidase_2 cd06419
Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) ...
 1-111 7.73e-03

Uncharacterized bacterial muramidase containing a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.


Pssm-ID: 119381  Cd Length: 190  Bit Score: 36.22  E-value: 7.73e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2295394060   1 MSLNGIDISHHQGEagMTVSIFEKMSFDFAIMKATDGTRFVDAWCDKYYQSAKKRGKLLGVYH-YANGTNAEKEVDYFLT 79
Cdd:cd06419     6 YPVLGVQLSQDDGY--IDFNSLQSNGISFVYLRATQGASYFDDNFLSNFSRAQGTGLSVGVIHtFSFSSTAAAQYRYFIR 83

                          90       100       110
                  ....*....|....*....|....*....|..
gi 2295394060  80 HCKNYVGEALIALdwEKSYNQSFdNPNWAKQW 111
Cdd:cd06419    84 KVGNNTGNLPIAI--YVSYYGDY-NPDTKKST 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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