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Conserved domains on  [gi|2437085822|gb|WCF44289|]
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polyprotein, partial [Rubus yellow net virus]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
ps-ssRNAv_RdRp-like super family cl40470
conserved catalytic core domain of RNA-dependent RNA polymerase (RdRp) from the positive-sense ...
1-23 5.85e-03

conserved catalytic core domain of RNA-dependent RNA polymerase (RdRp) from the positive-sense single-stranded RNA [(+)ssRNA] viruses and closely related viruses; This family contains the catalytic core domain of RdRp of RNA viruses which belong to Group IV of the Baltimore classification system, and are a group of related viruses that have positive-sense (+), single-stranded (ss) genomes made of ribonucleic acid (RNA). RdRp (also known as RNA replicase) catalyzes the replication of RNA from an RNA template; specifically, it catalyzes the synthesis of the RNA strand complementary to a given RNA template. The Baltimore Classification is divided into 7 classes, 3 of which include RNA viruses: Group IV (+) RNA viruses, Group III double-stranded (ds) RNA viruses, and Group V negative-sense (-) RNA viruses. Baltimore groups of viruses differ with respect to the nature of their genome (i.e., the nucleic acid form that is packaged into virions) and correspond to distinct strategies of genome replication and expression. (+) viral RNA is similar to mRNA and thus can be immediately translated by the host cell. (+)ssRNA viruses can also produce (+) copies of the genome from (-) strands of an intermediate dsRNA genome. This acts as both a transcription and a replication process since the replicated RNA is also mRNA. RdRps belong to the expansive class of polymerases containing so-called palm catalytic domains along with the accessory fingers and thumb domains. All RdRps also have six conserved structural motifs (A-F), located in its majority in the palm subdomain (A-E motifs) and the F motif is located on the finger subdomain. All these motifs have been shown to be implicated in RdRp fidelity such as processes of correct incorporation and reorganization of nucleotides. In addition to Group IV viruses, this model also includes Picobirnaviruses (PBVs), members of the family Picobirnaviridae of dsRNA viruses (Baltimore classification Group III), which are bi-segmented dsRNA viruses. The phylogenetic tree of the RdRps of RNA viruses (realm Riboviria) showed that picobirnaviruses are embedded in the branch of diverse (+)RNA viruses; sometimes they are collectively referred to as the picornavirus supergroup. RdRps of members of the family Permutatetraviridae, a distinct group of RNA viruses that encompass a circular permutation within the RdRp palm domain, are not included in this model.


The actual alignment was detected with superfamily member cd01647:

Pssm-ID: 477363  Cd Length: 177  Bit Score: 34.88  E-value: 5.85e-03
                          10        20
                  ....*....|....*....|...
gi 2437085822   1 RDNGLVLSPSKMKLAATEIDFLG 23
Cdd:cd01647   152 REAGLKLNPEKCEFGVPEVEFLG 174
RT_RNaseH_2 super family cl39038
RNase H-like domain found in reverse transcriptase;
99-135 7.14e-03

RNase H-like domain found in reverse transcriptase;


The actual alignment was detected with superfamily member pfam17919:

Pssm-ID: 465567 [Multi-domain]  Cd Length: 100  Bit Score: 34.01  E-value: 7.14e-03
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 2437085822  99 VKKIKSLVQNLPDLKLPSEEAYMIIETDGCMEGWGGV 135
Cdd:pfam17919   9 FEKLKQALTSAPVLAHPDPDKPFILETDASDYGIGAV 45
 
Name Accession Description Interval E-value
RT_LTR cd01647
RT_LTR: Reverse transcriptases (RTs) from retrotransposons and retroviruses which have long ...
1-23 5.85e-03

RT_LTR: Reverse transcriptases (RTs) from retrotransposons and retroviruses which have long terminal repeats (LTRs) in their DNA copies but not in their RNA template. RT catalyzes DNA replication from an RNA template, and is responsible for the replication of retroelements. An RT gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. RTs are present in a variety of mobile elements, including retrotransposons, retroviruses, group II introns, bacterial msDNAs, hepadnaviruses, and Caulimoviruses.


Pssm-ID: 238825  Cd Length: 177  Bit Score: 34.88  E-value: 5.85e-03
                          10        20
                  ....*....|....*....|...
gi 2437085822   1 RDNGLVLSPSKMKLAATEIDFLG 23
Cdd:cd01647   152 REAGLKLNPEKCEFGVPEVEFLG 174
RT_RNaseH_2 pfam17919
RNase H-like domain found in reverse transcriptase;
99-135 7.14e-03

RNase H-like domain found in reverse transcriptase;


Pssm-ID: 465567 [Multi-domain]  Cd Length: 100  Bit Score: 34.01  E-value: 7.14e-03
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 2437085822  99 VKKIKSLVQNLPDLKLPSEEAYMIIETDGCMEGWGGV 135
Cdd:pfam17919   9 FEKLKQALTSAPVLAHPDPDKPFILETDASDYGIGAV 45
 
Name Accession Description Interval E-value
RT_LTR cd01647
RT_LTR: Reverse transcriptases (RTs) from retrotransposons and retroviruses which have long ...
1-23 5.85e-03

RT_LTR: Reverse transcriptases (RTs) from retrotransposons and retroviruses which have long terminal repeats (LTRs) in their DNA copies but not in their RNA template. RT catalyzes DNA replication from an RNA template, and is responsible for the replication of retroelements. An RT gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. RTs are present in a variety of mobile elements, including retrotransposons, retroviruses, group II introns, bacterial msDNAs, hepadnaviruses, and Caulimoviruses.


Pssm-ID: 238825  Cd Length: 177  Bit Score: 34.88  E-value: 5.85e-03
                          10        20
                  ....*....|....*....|...
gi 2437085822   1 RDNGLVLSPSKMKLAATEIDFLG 23
Cdd:cd01647   152 REAGLKLNPEKCEFGVPEVEFLG 174
RT_RNaseH_2 pfam17919
RNase H-like domain found in reverse transcriptase;
99-135 7.14e-03

RNase H-like domain found in reverse transcriptase;


Pssm-ID: 465567 [Multi-domain]  Cd Length: 100  Bit Score: 34.01  E-value: 7.14e-03
                          10        20        30
                  ....*....|....*....|....*....|....*..
gi 2437085822  99 VKKIKSLVQNLPDLKLPSEEAYMIIETDGCMEGWGGV 135
Cdd:pfam17919   9 FEKLKQALTSAPVLAHPDPDKPFILETDASDYGIGAV 45
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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