NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446270865|ref|WP_000348720|]
View 

aminoglycoside 6'-N-acetyltransferase [Streptococcus pneumoniae]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 10456837)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate

CATH:  3.40.630.30
EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0008080
PubMed:  15581578|10940244|9175471|27367672|26818562
SCOP:  3000403

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
PhnO super family cl42766
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 70-135 3.46e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


The actual alignment was detected with superfamily member COG0454:

Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.69  E-value: 3.46e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446270865  70 SPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGFQEANRI 135
Cdd:COG0454   56 DKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERY 121
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 70-135 3.46e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.69  E-value: 3.46e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446270865  70 SPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGFQEANRI 135
Cdd:COG0454   56 DKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERY 121
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 13-129 5.48e-14

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 63.69  E-value: 5.48e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446270865   13 KLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFTEVEGDTIVdlALCSLRFDYVEGckysPVGFLEGIIVDEEYRLKDIAKN 92
Cdd:pfam00583   6 ELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELV--GFASLSIIDDEP----PVGEIEGLAVAPEYRGKGIGTA 79

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446270865   93 LCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGF 129
Cdd:pfam00583  80 LLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 46-109 2.54e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 2.54e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446270865  46 VEGDTIVdlALCSLRFDYVEGckysPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEF 109
Cdd:cd04301    5 EDDGEIV--GFASLSPDGSGG----DTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
PTZ00330 PTZ00330
acetyltransferase; Provisional
 72-134 5.44e-04

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 37.90  E-value: 5.44e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446270865  72 VGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTltnTDSIRFHLNIGFQEANR 134
Cdd:PTZ00330  82 VGHIEDVVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDCT---EDMVAFYKKLGFRACER 141
 
Name Accession Description Interval E-value
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 70-135 3.46e-14

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 64.69  E-value: 3.46e-14
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 446270865  70 SPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGFQEANRI 135
Cdd:COG0454   56 DKVLELKRLYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGNPAAIRFYERLGFKEIERY 121
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 13-129 5.48e-14

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 63.69  E-value: 5.48e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446270865   13 KLMKQLWPQLTDKEAIDEVKKYTNGKNTAIFTEVEGDTIVdlALCSLRFDYVEGckysPVGFLEGIIVDEEYRLKDIAKN 92
Cdd:pfam00583   6 ELLSEEFPEPWPDEPLDLLEDWDEDASEGFFVAEEDGELV--GFASLSIIDDEP----PVGEIEGLAVAPEYRGKGIGTA 79

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 446270865   93 LCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGF 129
Cdd:pfam00583  80 LLQALLEWARERGCERIFLEVAADNLAAIALYEKLGF 116
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
46-135 9.09e-10

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 53.85  E-value: 9.09e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446270865  46 VEGDTIVdlALCSLRfDYVEGCKYSPVGfLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHL 125
Cdd:COG1247   58 EEDGEVV--GFASLG-PFRPRPAYRGTA-EESIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADNEASIALYE 133

                         90
                 ....*....|
gi 446270865 126 NIGFQEANRI 135
Cdd:COG1247  134 KLGFEEVGTL 143
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 71-138 1.22e-09

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 51.97  E-value: 1.22e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 446270865  71 PVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTLTNTDSIRFHLNIGFQEANRIIHF 138
Cdd:COG0456   12 DEAEIEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEVREDNEAAIALYEKLGFEEVGERPNY 79
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
12-132 1.02e-07

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 47.77  E-value: 1.02e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446270865  12 AKLMKQLWPQLTDKEAIDEVKKytNGKNTAIFTEVEGDTIVdlalCSLRFDYVEGCKYSPVGFLEGIIVDEEYRLKDIAK 91
Cdd:COG3153   13 AALLRAAFGPGREAELVDRLRE--DPAAGLSLVAEDDGEIV----GHVALSPVDIDGEGPALLLGPLAVDPEYRGQGIGR 86

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 446270865  92 NLCTKCEEWAKNKGCKEFasdCTLTNTDSIRFHLNIGFQEA 132
Cdd:COG3153   87 ALMRAALEAARERGARAV---VLLGDPSLLPFYERFGFRPA 124
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 72-134 5.85e-07

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 45.75  E-value: 5.85e-07
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446270865  72 VGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFasdCTLTNTDSIRFHLNIGFQEANR 134
Cdd:COG1246   52 LAELRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRL---FLLTTSAAIHFYEKLGFEEIDK 111
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 46-109 2.54e-05

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 39.95  E-value: 2.54e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 446270865  46 VEGDTIVdlALCSLRFDYVEGckysPVGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEF 109
Cdd:cd04301    5 EDDGEIV--GFASLSPDGSGG----DTAYIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRL 62
PTZ00330 PTZ00330
acetyltransferase; Provisional
 72-134 5.44e-04

acetyltransferase; Provisional


Pssm-ID: 140351 [Multi-domain]  Cd Length: 147  Bit Score: 37.90  E-value: 5.44e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 446270865  72 VGFLEGIIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFASDCTltnTDSIRFHLNIGFQEANR 134
Cdd:PTZ00330  82 VGHIEDVVVDPSYRGQGLGRALISDLCEIARSSGCYKVILDCT---EDMVAFYKKLGFRACER 141
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 78-131 3.65e-03

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 34.35  E-value: 3.65e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 446270865   78 IIVDEEYRLKDIAKNLCTKCEEWAKNKGCKEFasdCTLTNTDSIRFHLNIGFQE 131
Cdd:pfam13508  34 LAVHPEYRGQGIGRALLEAAEAAAKEGGIKLL---ELETTNRAAAFYEKLGFEE 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH