NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|446273394|ref|WP_000351249|]
View 

MULTISPECIES: type II toxin-antitoxin system death-on-curing family toxin [Gammaproteobacteria]

Protein Classification

type II toxin-antitoxin system death-on-curing family toxin( domain architecture ID 10007658)

type II toxin-antitoxin system death-on-curing family toxin phosphorylates and inactivates elongation factor Tu

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Doc COG3654
Prophage maintenance system killer protein [Mobilome: prophages, transposons];
9-121 8.49e-26

Prophage maintenance system killer protein [Mobilome: prophages, transposons];


:

Pssm-ID: 442871  Cd Length: 130  Bit Score: 94.16  E-value: 8.49e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394   9 ERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGL-DDVFEIAAKYTACIAVSHALPDANKRTGLAVALEYLS 87
Cdd:COG3654    8 EDVLAIHDRLLAEYGGLPGVRDEGLLESALARPRQTFFGEELyPDLFEKAAALLYGIAKNHPFVDGNKRTAFAAALVFLD 87

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446273394  88 LNDFELTQENDLLADAVRDLVIGIINETDFADIL 121
Cdd:COG3654   88 LNGYELDADDDEAVDLVLAVAAGELDEEELAAWL 121
 
Name Accession Description Interval E-value
Doc COG3654
Prophage maintenance system killer protein [Mobilome: prophages, transposons];
9-121 8.49e-26

Prophage maintenance system killer protein [Mobilome: prophages, transposons];


Pssm-ID: 442871  Cd Length: 130  Bit Score: 94.16  E-value: 8.49e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394   9 ERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGL-DDVFEIAAKYTACIAVSHALPDANKRTGLAVALEYLS 87
Cdd:COG3654    8 EDVLAIHDRLLAEYGGLPGVRDEGLLESALARPRQTFFGEELyPDLFEKAAALLYGIAKNHPFVDGNKRTAFAAALVFLD 87

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446273394  88 LNDFELTQENDLLADAVRDLVIGIINETDFADIL 121
Cdd:COG3654   88 LNGYELDADDDEAVDLVLAVAAGELDEEELAAWL 121
DOC_P1 TIGR01550
death-on-curing family protein; The characterized member of this family is the death-on-curing ...
 9-102 3.02e-11

death-on-curing family protein; The characterized member of this family is the death-on-curing (DOC) protein of phage P1. It is part of a two protein operon with prevents-host-death (phd) that forms an addiction module. DOC lacks homology to analogous addiction module post-segregational killing proteins involved in plasmid maintenance. These modules work as a combination of a long lived poison (e.g. this protein) and a more abundant but shorter lived antidote. Members of this family have a well-conserved central motif HxFx[ND][AG]NKR. A similar region, with K replaced by G, is found in the huntingtin interacting protein (HYPE) family. [Unknown function, General]


Pssm-ID: 273687  Cd Length: 121  Bit Score: 56.32  E-value: 3.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394    9 ERVIEINAFILKTEPGM-KGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLAVALEYLS 87
Cdd:TIGR01550   5 EEIIAIHDANIERDGGLeFGMSNPGRAEATIERVSERLSYEESTDIFEVSAVLLYALIRSHPFNNANKRTALNALLLFLE 84

                          90
                  ....*....|....*
gi 446273394   88 LNDFELTQENDLLAD 102
Cdd:TIGR01550  85 LNGYEFTDSPEELID 99
Fic pfam02661
Fic/DOC family; This family consists of the Fic (filamentation induced by cAMP) protein and ...
 6-86 2.41e-06

Fic/DOC family; This family consists of the Fic (filamentation induced by cAMP) protein and doc (death on curing). The Fic protein is involved in cell division and is suggested to be involved in the synthesis of PAB or folate, indicating that the Fic protein and cAMP are involved in a regulatory mechanism of cell division via folate metabolism. This family contains a central conserved motif HPFXXGNG in most members. The exact molecular function of these proteins is uncertain. P1 lysogens of Escherichia coli carry the prophage as a stable low copy number plasmid. The frequency with which viable cells cured of prophage are produced is about 10(-5) per cell per generation. A significant part of this remarkable stability can be attributed to a plasmid-encoded mechanism that causes death of cells that have lost P1. In other words, the lysogenic cells appear to be addicted to the presence of the prophage. The plasmid withdrawal response depends on a gene named doc (death on curing) that is represented by this family. Doc induces a reversible growth arrest of E. coli cells by targetting the protein synthesis machinery. Doc hosts the C-terminal domain of its antitoxin partner Phd (prevents host death) through fold complementation, a domain that is intrinsically disordered in solution but that folds into an alpha-helix on binding to Doc.This domain forms complexes with Phd antitoxins containing pfam02604.


Pssm-ID: 426907  Cd Length: 94  Bit Score: 42.84  E-value: 2.41e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394    6 FPFERVIEINAFILKTEPGMKGAVDIP---KLQGALGRIDNAIVY---------EGLDDVFEIAAKYTACIAVSHALPDA 73
Cdd:pfam02661   2 LDLEDLLALHRLLIERHGGAGGARDVNvsgLLESALARPEQIPFGleelllypdLDREHPLEKAAALHFGFAKIHPFRDG 81

                          90
                  ....*....|...
gi 446273394   74 NKRTGLAVALEYL 86
Cdd:pfam02661  82 NGRTARLLANLFL 94
 
Name Accession Description Interval E-value
Doc COG3654
Prophage maintenance system killer protein [Mobilome: prophages, transposons];
9-121 8.49e-26

Prophage maintenance system killer protein [Mobilome: prophages, transposons];


Pssm-ID: 442871  Cd Length: 130  Bit Score: 94.16  E-value: 8.49e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394   9 ERVIEINAFILKTEPGMKGAVDIPKLQGALGRIDNAIVYEGL-DDVFEIAAKYTACIAVSHALPDANKRTGLAVALEYLS 87
Cdd:COG3654    8 EDVLAIHDRLLAEYGGLPGVRDEGLLESALARPRQTFFGEELyPDLFEKAAALLYGIAKNHPFVDGNKRTAFAAALVFLD 87

                         90       100       110
                 ....*....|....*....|....*....|....
gi 446273394  88 LNDFELTQENDLLADAVRDLVIGIINETDFADIL 121
Cdd:COG3654   88 LNGYELDADDDEAVDLVLAVAAGELDEEELAAWL 121
DOC_P1 TIGR01550
death-on-curing family protein; The characterized member of this family is the death-on-curing ...
 9-102 3.02e-11

death-on-curing family protein; The characterized member of this family is the death-on-curing (DOC) protein of phage P1. It is part of a two protein operon with prevents-host-death (phd) that forms an addiction module. DOC lacks homology to analogous addiction module post-segregational killing proteins involved in plasmid maintenance. These modules work as a combination of a long lived poison (e.g. this protein) and a more abundant but shorter lived antidote. Members of this family have a well-conserved central motif HxFx[ND][AG]NKR. A similar region, with K replaced by G, is found in the huntingtin interacting protein (HYPE) family. [Unknown function, General]


Pssm-ID: 273687  Cd Length: 121  Bit Score: 56.32  E-value: 3.02e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394    9 ERVIEINAFILKTEPGM-KGAVDIPKLQGALGRIDNAIVYEGLDDVFEIAAKYTACIAVSHALPDANKRTGLAVALEYLS 87
Cdd:TIGR01550   5 EEIIAIHDANIERDGGLeFGMSNPGRAEATIERVSERLSYEESTDIFEVSAVLLYALIRSHPFNNANKRTALNALLLFLE 84

                          90
                  ....*....|....*
gi 446273394   88 LNDFELTQENDLLAD 102
Cdd:TIGR01550  85 LNGYEFTDSPEELID 99
Fic pfam02661
Fic/DOC family; This family consists of the Fic (filamentation induced by cAMP) protein and ...
 6-86 2.41e-06

Fic/DOC family; This family consists of the Fic (filamentation induced by cAMP) protein and doc (death on curing). The Fic protein is involved in cell division and is suggested to be involved in the synthesis of PAB or folate, indicating that the Fic protein and cAMP are involved in a regulatory mechanism of cell division via folate metabolism. This family contains a central conserved motif HPFXXGNG in most members. The exact molecular function of these proteins is uncertain. P1 lysogens of Escherichia coli carry the prophage as a stable low copy number plasmid. The frequency with which viable cells cured of prophage are produced is about 10(-5) per cell per generation. A significant part of this remarkable stability can be attributed to a plasmid-encoded mechanism that causes death of cells that have lost P1. In other words, the lysogenic cells appear to be addicted to the presence of the prophage. The plasmid withdrawal response depends on a gene named doc (death on curing) that is represented by this family. Doc induces a reversible growth arrest of E. coli cells by targetting the protein synthesis machinery. Doc hosts the C-terminal domain of its antitoxin partner Phd (prevents host death) through fold complementation, a domain that is intrinsically disordered in solution but that folds into an alpha-helix on binding to Doc.This domain forms complexes with Phd antitoxins containing pfam02604.


Pssm-ID: 426907  Cd Length: 94  Bit Score: 42.84  E-value: 2.41e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 446273394    6 FPFERVIEINAFILKTEPGMKGAVDIP---KLQGALGRIDNAIVY---------EGLDDVFEIAAKYTACIAVSHALPDA 73
Cdd:pfam02661   2 LDLEDLLALHRLLIERHGGAGGARDVNvsgLLESALARPEQIPFGleelllypdLDREHPLEKAAALHFGFAKIHPFRDG 81

                          90
                  ....*....|...
gi 446273394   74 NKRTGLAVALEYL 86
Cdd:pfam02661  82 NGRTARLLANLFL 94
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH