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Conserved domains on  [gi|504289042|ref|WP_014476144|]
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MULTISPECIES: NAD(P)H-dependent oxidoreductase [Bacillus]

Protein Classification

NAD(P)H-dependent oxidoreductase( domain architecture ID 10114874)

NAD(P)H-dependent oxidoreductase, similar to nitroreductase NfsB, which catalyzes the reduction of flavin or nitrocompounds using NAD(P)H as electron donor

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-217 8.42e-80

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


:

Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 235.61  E-value: 8.42e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   7 QILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  87 ARTakdikynadyikrhlkevkqmpqdvyegylskteefqkndlhllesdrtlfDWASKQTYIALGNMMTAAAQIGVDSC 166
Cdd:cd02149   81 AKK---------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSC 109

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504289042 167 PIEGFQYdhiHRILEEEGLLENGsFDISVMVAFGYRVRDPRPKTRSAVEDV 217
Cdd:cd02149  110 PIEGFDP---AKLDEILGLDEKG-YKISVMVAFGYRSEEKLPKSRKPLEDV 156
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-217 8.42e-80

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 235.61  E-value: 8.42e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   7 QILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  87 ARTakdikynadyikrhlkevkqmpqdvyegylskteefqkndlhllesdrtlfDWASKQTYIALGNMMTAAAQIGVDSC 166
Cdd:cd02149   81 AKK---------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSC 109

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504289042 167 PIEGFQYdhiHRILEEEGLLENGsFDISVMVAFGYRVRDPRPKTRSAVEDV 217
Cdd:cd02149  110 PIEGFDP---AKLDEILGLDEKG-YKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-218 2.97e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.68  E-value: 2.97e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   8 ILDAYNFRHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE-YTWGAQKQLPTASHFVLIL 86
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  87 ARTAKdikynadyikrhlkevkqmpqdvyegylskteefqkndlhlleSDRTLFDWASKQTYIALGNMMTAAAQIGVDSC 166
Cdd:COG0778   80 ADPDR-------------------------------------------SEKVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 504289042 167 PIEGFQYDHIHRILeeeGLLENgsFDISVMVAFGYRVRDPRPKTRSAVEDVV 218
Cdd:COG0778  117 WIGGFDPEKVRELL---GLPEG--EEPVALLALGYPAEELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
8-217 6.91e-38

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 130.86  E-value: 6.91e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   8 ILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE-----YTWGAQKQLpTASHF 82
Cdd:PRK11053   3 IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKIL-DASHV 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  83 VLILARTAKDIKYNADYIKRHLK-------EVKQMPQDVYEGYLskteEFQKNDLhllesdRTLFDWASKQTYIALGNMM 155
Cdd:PRK11053  82 VVFCAKTDMDDAYLELVLEQEDAdgrfateEAKAAQDKGRRFFA----DMHRKEL------KDLQHWMEKQVYLALGNLL 151

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 504289042 156 TAAAQIGVDSCPIEGFQydhiHRILEEE-GLLENGsFDISVMVAFGYRVRD----PRPKTRSAVEDV 217
Cdd:PRK11053 152 LGAAALGIDATPIEGFD----AAILDAEfGLREKG-LTSSVVVPLGYHSEEdfnaKLPKSRLPQETI 213
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-201 9.28e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 105.94  E-value: 9.28e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   15 RHATKEFDPNKkVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTAshFVLILARTAKDIK 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAA--ALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   95 YNADYIKRhlkevkQMPQDVyegYLSKTEEFQKNDlhlleSDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYD 174
Cdd:pfam00881  81 LLLQDFLR------GAPVLI---VITASLSTYLRK-----AAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAA 146

                         170       180
                  ....*....|....*....|....*..
gi 504289042  175 HIHRILeeeGLLENGsfDISVMVAFGY 201
Cdd:pfam00881 147 AVRELL---GLPDDE--RLVGLIAVGY 168
 
Name Accession Description Interval E-value
NfsB-like cd02149
nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This ...
 7-217 8.42e-80

nitroreductase similar to Escherichia coli NfsB; NAD(P)H:FMN oxidoreductase family. This domain catalyzes the reduction of flavin, nitrocompound, quinones and azo compounds using NADH or NADPH as an electron donor. The enzyme is a homodimer, and each monomer binds a FMN as co-factor. This family includes FRase I in Vibrio fischeri, wihich reduces FMN into FMNH2 as part of the bioluminescent reaction. The family also includes oxygen-insensitive nitroreductases that use NADH or NADPH as an electron donor in the ping pong bi bi mechanism. This type of nitroreductase can be used in cancer chemotherapy to activate a range of prodrugs.


Pssm-ID: 380324 [Multi-domain]  Cd Length: 156  Bit Score: 235.61  E-value: 8.42e-80
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   7 QILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLIL 86
Cdd:cd02149    1 NILELLNFRYATKKFDPNKKISDEDLETILEALRLSPSSFGLEPWKFLVVENPELKAKLAPAAWFNQPQIKDASHVVVFL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  87 ARTakdikynadyikrhlkevkqmpqdvyegylskteefqkndlhllesdrtlfDWASKQTYIALGNMMTAAAQIGVDSC 166
Cdd:cd02149   81 AKK---------------------------------------------------DWSAKQTYIALGNMLLAAAMLGIDSC 109

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504289042 167 PIEGFQYdhiHRILEEEGLLENGsFDISVMVAFGYRVRDPRPKTRSAVEDV 217
Cdd:cd02149  110 PIEGFDP---AKLDEILGLDEKG-YKISVMVAFGYRSEEKLPKSRKPLEDV 156
NfnB COG0778
Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway ...
 8-218 2.97e-41

Nitroreductase [Energy production and conversion]; Nitroreductase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440541 [Multi-domain]  Cd Length: 163  Bit Score: 137.68  E-value: 2.97e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   8 ILDAYNFRHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE-YTWGAQKQLPTASHFVLIL 86
Cdd:COG0778    1 LLELLLTRRSVRKFTD-KPVSDEELEELLEAARLAPSAGNLQPWRFVVVRDPELRERLAEaLAEANQEWVADAPVLIVVC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  87 ARTAKdikynadyikrhlkevkqmpqdvyegylskteefqkndlhlleSDRTLFDWASKQTYIALGNMMTAAAQIGVDSC 166
Cdd:COG0778   80 ADPDR-------------------------------------------SEKVPERYALLDAGIAAQNLLLAARALGLGTC 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 504289042 167 PIEGFQYDHIHRILeeeGLLENgsFDISVMVAFGYRVRDPRPKTRSAVEDVV 218
Cdd:COG0778  117 WIGGFDPEKVRELL---GLPEG--EEPVALLALGYPAEELNPRPRKPLEEVV 163
PRK11053 PRK11053
oxygen-insensitive NAD(P)H nitroreductase;
8-217 6.91e-38

oxygen-insensitive NAD(P)H nitroreductase;


Pssm-ID: 182929 [Multi-domain]  Cd Length: 217  Bit Score: 130.86  E-value: 6.91e-38
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   8 ILDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE-----YTWGAQKQLpTASHF 82
Cdd:PRK11053   3 IVSVAKKRYTTKAFDPSKKLPAEQIEQIKTLLRFSPSSVNSQPWHFIVASTEEGKARIAKaaagnYAFNERKIL-DASHV 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  83 VLILARTAKDIKYNADYIKRHLK-------EVKQMPQDVYEGYLskteEFQKNDLhllesdRTLFDWASKQTYIALGNMM 155
Cdd:PRK11053  82 VVFCAKTDMDDAYLELVLEQEDAdgrfateEAKAAQDKGRRFFA----DMHRKEL------KDLQHWMEKQVYLALGNLL 151

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 504289042 156 TAAAQIGVDSCPIEGFQydhiHRILEEE-GLLENGsFDISVMVAFGYRVRD----PRPKTRSAVEDV 217
Cdd:PRK11053 152 LGAAALGIDATPIEGFD----AAILDAEfGLREKG-LTSSVVVPLGYHSEEdfnaKLPKSRLPQETI 213
Nitroreductase pfam00881
Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent ...
 15-201 9.28e-29

Nitroreductase family; The nitroreductase family comprises a group of FMN- or FAD-dependent and NAD(P)H-dependent enzymes able to metabolize nitrosubstituted compounds.


Pssm-ID: 425926 [Multi-domain]  Cd Length: 168  Bit Score: 105.94  E-value: 9.28e-29
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   15 RHATKEFDPNKkVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTAshFVLILARTAKDIK 94
Cdd:pfam00881   4 RRSVRKFDPEP-VPKEVLEEILEAARRAPSAGNLQPWRFYVVTDGELRYRLAEAALELLLVEPAA--ALLLLLRRDANLK 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   95 YNADYIKRhlkevkQMPQDVyegYLSKTEEFQKNDlhlleSDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYD 174
Cdd:pfam00881  81 LLLQDFLR------GAPVLI---VITASLSTYLRK-----AAERAYREALLDAGAAAQNLLLAATSLGLGSCPIGGFDAA 146

                         170       180
                  ....*....|....*....|....*..
gi 504289042  175 HIHRILeeeGLLENGsfDISVMVAFGY 201
Cdd:pfam00881 147 AVRELL---GLPDDE--RLVGLIAVGY 168
MhqN-like cd02137
nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily ...
 9-217 2.21e-26

nitroreductase family protein similar to the NAD(P)H nitroreductase MhqN; A diverse subfamily of the nitroreductase family containing uncharacterized proteins; includes nitroreductases MhqN, YodC, YdgI, DrgA. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380314 [Multi-domain]  Cd Length: 147  Bit Score: 99.24  E-value: 2.21e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   9 LDAYNFRHATKEFDPNKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGaQKQLPTASHFVLILAR 88
Cdd:cd02137    1 LEVIKSRRSVRNFDPDHKIPKEELKEILELATLAPSSFNLQPWRFVVVRDPELKAKLAEAAYN-QPQVTTASAVILVLGD 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  89 TakdikyNADyikrhlkevkqmpqdvyegylskteefqkndlhllesdrtlfdwaskqtyIALGNMMTAAAQIGVDSCPI 168
Cdd:cd02137   80 L------NAG--------------------------------------------------LAAMNLMLAAKAKGYDTCPM 103

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*....
gi 504289042 169 EGFQYDHIHRILEeeglLENGsFDISVMVAFGYRVRDPRPKTRSAVEDV 217
Cdd:cd02137  104 GGFDKEKVAELLN----LPDR-YVPVLLIAIGKAADKAPRSGRLPVDEV 147
nitroreductase cd03370
uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus ...
 27-215 8.71e-22

uncharacterized nitroreductase family proteins; Nitroreductase family containing Thermus thermophilus NADH oxidase and other, uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380327 [Multi-domain]  Cd Length: 191  Bit Score: 88.53  E-value: 8.71e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  27 VSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGaQKQLpTASHFVLILARTAKDIKYNADYI------ 100
Cdd:cd03370   19 VPDEDLREILRLAGLAPSAWNIQPWRFVVVRDAELKEQLQAAAYG-QAQV-TSAPAVIVIYSDMEDALANLEETihpgls 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042 101 -KRHLKEVKQMpQDVYEGYlskTEEFQKNdlhllesdrtlfdWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYDHIHRI 179
Cdd:cd03370   97 eERRQREAAGL-RGAFGKM---SVEQRGQ-------------WGLAQANIALGFLLLAAQSLGYDTSPMLGFDPEKVKAL 159

                        170       180       190
                 ....*....|....*....|....*....|....*.
gi 504289042 180 LeeeGLLENGSfdISVMVAFGYRVRDPRPKTRSAVE 215
Cdd:cd03370  160 L---GLPEHVT--IAALVALGKPAEEGYPHHRHSLE 190
Nitro_FMN_reductase cd02062
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-201 4.84e-18

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380311 [Multi-domain]  Cd Length: 139  Bit Score: 76.95  E-value: 4.84e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  15 RHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLILARtakdik 94
Cdd:cd02062    4 RRSIRKFTD-KPVPEEKLRKILEAARLAPSAGNLQPWRFIVVRDREKKEKLAKLAAPNQKFIAGAPVVIVVVAD------ 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  95 ynadyikrhlkevkqmpqdvyegylskteefqkndlhllesDRTLFDWASKQTYIALGNMMTAAAQIGVDSCPIEGFQYD 174
Cdd:cd02062   77 -----------------------------------------PDKSRPWALEDAGAAAQNLLLAAAALGLGSCWIGGFDFR 115

                        170       180
                 ....*....|....*....|....*..
gi 504289042 175 HiHRILEEEGLLENgsFDISVMVAFGY 201
Cdd:cd02062  116 E-DKVRELLGIPEN--LRPVALIAIGY 139
PnbA_NfnB-like cd02136
nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as ...
 15-221 1.13e-12

nitroreductase similar to Mycobacterium smegmatis NfnB; Members of this family utilize FMN as a cofactor and catalyze reduction of a variety of nitroaromatic compounds, including nitrofurans, nitrobenzens, nitrophenol, nitrobenzoate and quinones by using either NADH or NADPH as a source of reducing equivalents in an obligatory two-election transfer mechanism. The enzyme is typically a homodimer. Mycobacterium smegmatis nitroreductase NfnB plays a role in resistance to benzothiazinone.


Pssm-ID: 380313 [Multi-domain]  Cd Length: 152  Bit Score: 62.99  E-value: 1.13e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  15 RHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEfREKLREYTWGAqkqlPTAshfvLILartakdik 94
Cdd:cd02136    5 RRSVRAFKD-KPVPKETIEKILEAARRAPSGKNTQPWRVYVVTGKA-RERLKKAFFGA----PVA----LFL-------- 66

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  95 ynadyikrhlkevkqmpqdvyegYLSKTEEFQkndlhllesdrTLFDWAskqtyIALGNMMTAAAQIGVDSCPIEGFQYD 174
Cdd:cd02136   67 -----------------------TMDKVLGPW-----------SWFDLG-----AFLQNLMLAAHALGLGTCPQGALAGY 107

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*..
gi 504289042 175 HiHRILEEEGLLENGSFDISVMVAFGYRVrDPRPKTRSAVEDVVKWV 221
Cdd:cd02136  108 P-DVVRKELGIPDDEELVCGIALGYPDPD-APVNQFRTPREPLEEFV 152
nitroreductase cd02139
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 8-220 1.19e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380316 [Multi-domain]  Cd Length: 165  Bit Score: 57.87  E-value: 1.19e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   8 ILDAYNFRHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGaQKQLPTAShfVLILA 87
Cdd:cd02139    1 VYEAIKKRRSIRKYKP-TPVEEEKLLRILEAARLAPSAKNRQPWRFIVVKDKELKEKLAEAANG-QKFIAEAP--VVIVA 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  88 RTAKDIKYNADYIKRHlkevkqmpqdvyegylskteefqkndlhllesdrtLFDWAskqtyIALGNMMTAAAQIGVDSCP 167
Cdd:cd02139   77 CADPSESGMGCGKPYY-----------------------------------LVDVA-----IAMEHLVLAATEEGLGTCW 116

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504289042 168 IEGFQYDHIHRILeeeGLLENgsFDISVMVAFGYRVRDPRPKTRSAVEDVVKW 220
Cdd:cd02139  117 IGAFDEDKVKEIL---GIPEE--YRVVALTPLGYPAEEPPPRPRKPLEEIVFY 164
nitroreductase cd20608
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 9-201 2.48e-10

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380329 [Multi-domain]  Cd Length: 145  Bit Score: 56.57  E-value: 2.48e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042   9 LDAYNFRHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTAShfVLILAr 88
Cdd:cd20608    1 FEAIKTRRSVRRFSD-KPVEEEKLEKILEAARLAPSWANKQCWRFIVVTDKETLSELAKKESPSNGWLKDAP--VIIVV- 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  89 takdikyNADyikrhlkevkqmPQDvyegylSKTEEFQkndlhllesDRTLFDWAskqtyIALGNMMTAAAQIGVDSCPI 168
Cdd:cd20608   77 -------CAD------------PKD------SGWLNGQ---------NYYLVDAA-----IAMQNLMLAATDLGLGTCWI 117

                        170       180       190
                 ....*....|....*....|....*....|...
gi 504289042 169 EGFQYDHIHRILeeeGLLENgsFDISVMVAFGY 201
Cdd:cd20608  118 GAFDEKKVKEIL---GIPEN--IRVVALTPLGY 145
nitroreductase cd20609
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 15-67 2.50e-10

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380330 [Multi-domain]  Cd Length: 145  Bit Score: 56.63  E-value: 2.50e-10
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 504289042  15 RHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE 67
Cdd:cd20609    9 RYSVRKFSD-KPVEKEKLDKILEAGRLAPTAVNYQPQRILVVRSEEALEKLAK 60
TdsD-like cd02138
nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase ...
 15-218 2.50e-10

nitroreductase similar to Burkholderia pseudomallei TdsD; A subfamily of the nitroreductase family containing uncharacterized proteins that are similar to Burkholderia pseudomallei TdsD, may be involved in the processing of organosulfur compounds. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.


Pssm-ID: 380315 [Multi-domain]  Cd Length: 174  Bit Score: 57.17  E-value: 2.50e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  15 RHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVV-QNPEFREKLREY------TWgAQKqlptASHFVLILA 87
Cdd:cd02138    5 RWSPRAFSP-EPISEEDLLSLFEAARWAPSCFNEQPWRFVVArRDTEAFEKLLDLlaegnqSW-AKN----APVLIVVLA 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  88 RT--AKDIKYNADYikrhlkevkqmpqdvyegylskteefqkndlhllesdrtLFDwaskqTYIALGNMMTAAAQIGVDS 165
Cdd:cd02138   79 KTefDHNGKPNRYA---------------------------------------LFD-----TGAAVANLALQATALGLVV 114

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 504289042 166 CPIEGFQYDHIHRILeeeGLLENgsFDISVMVAFGY-----------RVRDPRPKTRSAVEDVV 218
Cdd:cd02138  115 HQMAGFDPEKAKEAL---GIPDE--YEPITMIAIGYpgdpeslpeklLEREEAPRTRKPLSEIV 173
nitroreductase cd02151
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 24-91 1.08e-09

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers..


Pssm-ID: 380326 [Multi-domain]  Cd Length: 157  Bit Score: 55.23  E-value: 1.08e-09
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 504289042  24 NKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKQLPTASHFVLILARTAK 91
Cdd:cd02151   14 DEPIEEEKLEEILEAALLAPSSRNSRPVEFIVVDDKETLKKLSECKPHGSAFLKGAPAAIVVLADTEK 81
NfsA-like cd02146
nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin ...
 24-218 1.62e-08

nitroreductase similar to Escherichia coli NfsA; This family contains NADPH-dependent flavin reductase and oxygen-insensitive nitroreductase. These enzymes are homodimeric flavoproteins that contain one FMN per monomer as a cofactor. Flavin reductase catalyzes the reduction of flavin by using NADPH as an electron donor. Oxygen-insensitive nitroreductase, such as NfsA protein in Escherichia coli, catalyzes reduction of nitrocompounds using NADPH as electron donor.


Pssm-ID: 380322 [Multi-domain]  Cd Length: 229  Bit Score: 53.01  E-value: 1.62e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042  24 NKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTwGAQKQLPTASHFVLILArtakdikynaDYiKRH 103
Cdd:cd02146   16 DEPLTDETLETLIAAAQSASTSSNLQAYSVIVVTDPELREKLAELA-GNQPYVAQAPVFLVFCA----------DL-YRH 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 504289042 104 LK--EVKQMPQDVYEGylskteefqkndlhlleSDRTLFdwASKQTYIALGNMMTAAAQIGVDSCPIEGFQyDHIHRILE 181
Cdd:cd02146   84 QKiaEEAGGKDVGLDY-----------------LESFLV--GVVDAALAAQNALVAAESLGLGIVYIGGIR-NNPEEVIE 143

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 504289042 182 EEGLLEnGSFDISVMvAFGYRVRDPRPKTRSAVEDVV 218
Cdd:cd02146  144 LLGLPE-YVFPLFGL-TVGHPDPTPEVKPRLPLEAVV 178
nitroreductase cd02150
nitroreductase family protein; A subfamily of the nitroreductase family containing ...
 24-91 9.71e-08

nitroreductase family protein; A subfamily of the nitroreductase family containing uncharacterized proteins. Nitroreductase catalyzes the reduction of nitroaromatic compounds such as nitrotoluenes, nitrofurans and nitroimidazoles. This process requires NAD(P)H as electron donor in an obligatory two-electron transfer and uses FMN as cofactor. The enzyme is typically a homodimer.often found to be homodimers.


Pssm-ID: 380325 [Multi-domain]  Cd Length: 156  Bit Score: 49.91  E-value: 9.71e-08
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 504289042  24 NKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLREYTWGAQKqLPTASHFVLILARTAK 91
Cdd:cd02150   12 DKPVEEEDIEKLLRAAMAAPSAGNQQPWHFIVVTDREKLDKIAEAHPYGKM-LKEAPLAIVVCGDPSK 78
iodotyrosine_dehalogenase cd02144
iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the ...
 24-67 3.19e-07

iodotyrosine dehalogenase; Iodotyrosine dehalogenase catalyzes the removal of iodine from the 3, 5 positions of L-tyosine in thyroid, liver and kidney, using NADPH as electron donor. This enzyme is a homolog of the nitroreductase family. These enzymes are usually homodimers.


Pssm-ID: 380320  Cd Length: 192  Bit Score: 48.69  E-value: 3.19e-07
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 504289042  24 NKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKLRE 67
Cdd:cd02144   16 SEPVPREVIENAIRTAGTAPSGANTQPWTFVVVSDPEIKRKIRE 59
nitroreductase cd20610
nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or ...
 15-65 4.68e-04

nitroreductase family protein; Proteins of this family catalyze the reduction of flavin or nitrocompounds using NAD(P)H as electron donor in a obligatory two-electron transfer, utilizing FMN or FAD as cofactor. They are often found to be homodimers. Enzymes of this family are described as NAD(P)H:FMN oxidoreductases, oxygen-insensitive nitroreductase, flavin reductase P, dihydropteridine reductase, NADH oxidase or NADH dehydrogenase.


Pssm-ID: 380331 [Multi-domain]  Cd Length: 167  Bit Score: 39.57  E-value: 4.68e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 504289042  15 RHATKEFDPnKKVSDSDFEFILETGRLSPSSLGLEPWKFVVVQNPEFREKL 65
Cdd:cd20610    4 RRSIRKFKP-DPVPKEDIEKILEAANWAPSGMNRQNWEFVVVKGGEKIEKI 53
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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