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Conserved domains on  [gi|505489704|ref|WP_015674350|]
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biotin--[acetyl-CoA-carboxylase] ligase [Actinobacillus suis]

Protein Classification

lipoate--protein ligase family protein( domain architecture ID 46944)

lipoate--protein ligase family protein, similar to Staphylococcus aureus lipoate--protein ligase 1 and Saccharomyces cerevisiae octanoyltransferase

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BPL_LplA_LipB super family cl14057
biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), ...
 1-255 6.35e-98

biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.


The actual alignment was detected with superfamily member PRK11886:

Pssm-ID: 449326 [Multi-domain]  Cd Length: 319  Bit Score: 288.99  E-value: 6.35e-98
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   1 MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTED 80
Cdd:PRK11886  63 LDLLDPERISSQLPPGRVTVLPVIDSTNQYLLDRIAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWR-LNQG 141

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  81 AAHISSLSLVISLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQA 159
Cdd:PRK11886 142 PAQAMGLSLVVGIAIAEALRRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAaHVVIGIGINVAMPDFPEELIDQP 221

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 160 WSDLSDYQ--FDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQ 237
Cdd:PRK11886 222 WSDLQEAGptIDRNQLAAELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKLIIGDKEISGIARGIDEQGALLLED 301

                        250
                 ....*....|....*...
gi 505489704 238 NSVITPFGIGEMSLRSDE 255
Cdd:PRK11886 302 DGVEKPFNGGEISLRSWE 319
 
Name Accession Description Interval E-value
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
1-255 6.35e-98

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 288.99  E-value: 6.35e-98
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   1 MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTED 80
Cdd:PRK11886  63 LDLLDPERISSQLPPGRVTVLPVIDSTNQYLLDRIAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWR-LNQG 141

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  81 AAHISSLSLVISLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQA 159
Cdd:PRK11886 142 PAQAMGLSLVVGIAIAEALRRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAaHVVIGIGINVAMPDFPEELIDQP 221

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 160 WSDLSDYQ--FDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQ 237
Cdd:PRK11886 222 WSDLQEAGptIDRNQLAAELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKLIIGDKEISGIARGIDEQGALLLED 301

                        250
                 ....*....|....*...
gi 505489704 238 NSVITPFGIGEMSLRSDE 255
Cdd:PRK11886 302 DGVEKPFNGGEISLRSWE 319
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 20-252 2.22e-89

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 264.34  E-value: 2.22e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  20 VFDEIDSTNEYLLKH-YQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:COG0340    4 VFDEVDSTNDEAKELaREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLR-PDLPPARLPLLSLAAGLAVAEA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  99 LQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLSDY---QFDRNALV 174
Cdd:COG0340   83 LRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIdWVVIGIGINVNQPPFDPEELDQPATSLKEEtgkEVDREELL 162

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505489704 175 CRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQNS-VITPFGIGEMSLR 252
Cdd:COG0340  163 AALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETGGETLEGIAVGIDEDGALLLETADgEIRAVAAGEVSLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 20-188 1.64e-60

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 188.63  E-value: 1.64e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  20 VFDEIDSTNEYLLKHYQTL-PQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:cd16442    4 VLDEIDSTNDEAKELARSGaPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLR-PDVPPAEAPLLTLLAAVAVAEA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  99 LQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLSDYQFDRNALVCRL 177
Cdd:cd16442   83 LEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVaAVVIGIGINVNNTPPPEPLPDTSLATSLGKEVDRNELLEEL 162

                        170
                 ....*....|.
gi 505489704 178 AYELQKNLKIY 188
Cdd:cd16442  163 LAALENRLELF 173
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 20-252 4.90e-58

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 184.53  E-value: 4.90e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   20 VFDEIDSTNEYLLKHYQTL-PQGSVCLAEQQTAGRGRRGRTWYSPQSeNVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:TIGR00121   4 VLDVIDSTNQYALELAKEGkLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILR-PDLPKSPAPGLTLVAGIAIAEV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   99 LQaQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLS--DYQFDRNALVC 175
Cdd:TIGR00121  82 LK-ELGDQVQVKWPNDILLKDKKLGGILTELTGKENRAdYVVIGIGINVQNRKPAESLREQAISLSEeaGIDLDRGELIE 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 505489704  176 RLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQNSVITPFGIGEMSLR 252
Cdd:TIGR00121 161 GFLRNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIEGIARGIDKDGALLLEDGGGIKKIISGEISLR 237
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 20-145 2.44e-23

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 91.73  E-value: 2.44e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   20 VFDEIDSTNEYLLKHYQTLPQ-GSVCLAEQQTAGRGRRGRTWYSPQSEnVYFSILW--HYPTEDAAHISSLSLVISLIIA 96
Cdd:pfam03099   1 LGERIKSTNTYLEELNSSELEsGGVVVVRRQTGGRGRGGNVWHSPKGC-LTYSLLLskEHPNVDPSVLEFYVLELVLAVL 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 505489704   97 ESL--QAQQVENI--QIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLN 145
Cdd:pfam03099  80 EALglYKPGISGIpcFVKWPNDLYVNGRKLAGILQRSTRGGTLHHGVIGLGVN 132
 
Name Accession Description Interval E-value
PRK11886 PRK11886
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;
1-255 6.35e-98

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA;


Pssm-ID: 237010 [Multi-domain]  Cd Length: 319  Bit Score: 288.99  E-value: 6.35e-98
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   1 MSKLNQSQIQTALLCGQALVFDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTED 80
Cdd:PRK11886  63 LDLLDPERISSQLPPGRVTVLPVIDSTNQYLLDRIAELKSGDLCLAEYQTAGRGRRGRQWFSPFGGNLYLSLYWR-LNQG 141

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  81 AAHISSLSLVISLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQA 159
Cdd:PRK11886 142 PAQAMGLSLVVGIAIAEALRRLGAIDVGLKWPNDIYLNDRKLAGILVELSGETGDAaHVVIGIGINVAMPDFPEELIDQP 221

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 160 WSDLSDYQ--FDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQ 237
Cdd:PRK11886 222 WSDLQEAGptIDRNQLAAELIKQLRAALELFEQEGLAPFLERWKKLDLFLGREVKLIIGDKEISGIARGIDEQGALLLED 301

                        250
                 ....*....|....*...
gi 505489704 238 NSVITPFGIGEMSLRSDE 255
Cdd:PRK11886 302 DGVEKPFNGGEISLRSWE 319
BirA2 COG0340
Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA ...
 20-252 2.22e-89

Biotin-(acetyl-CoA carboxylase) ligase [Coenzyme transport and metabolism]; Biotin-(acetyl-CoA carboxylase) ligase is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 440109 [Multi-domain]  Cd Length: 241  Bit Score: 264.34  E-value: 2.22e-89
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  20 VFDEIDSTNEYLLKH-YQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:COG0340    4 VFDEVDSTNDEAKELaREGAPEGTVVVAEEQTAGRGRRGRSWVSPPGKGLYFSLLLR-PDLPPARLPLLSLAAGLAVAEA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  99 LQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLSDY---QFDRNALV 174
Cdd:COG0340   83 LRELTGVDVGLKWPNDILLNGKKLAGILIEASGEGDGIdWVVIGIGINVNQPPFDPEELDQPATSLKEEtgkEVDREELL 162

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 505489704 175 CRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQNS-VITPFGIGEMSLR 252
Cdd:COG0340  163 AALLEELEELYDRFLEEGFAPILEEWRARLATLGRRVRVETGGETLEGIAVGIDEDGALLLETADgEIRAVAAGEVSLR 241
BPL cd16442
biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an ...
 20-188 1.64e-60

biotin protein ligase; Biotin protein ligase (EC 6.3.4.15) catalyzes the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine.


Pssm-ID: 319741 [Multi-domain]  Cd Length: 173  Bit Score: 188.63  E-value: 1.64e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  20 VFDEIDSTNEYLLKHYQTL-PQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:cd16442    4 VLDEIDSTNDEAKELARSGaPEGTVVVAEEQTAGRGRRGRKWESPKGKGLYFSLLLR-PDVPPAEAPLLTLLAAVAVAEA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  99 LQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLSDYQFDRNALVCRL 177
Cdd:cd16442   83 LEKLGGIPVQIKWPNDILVNGKKLAGILTEASAEGEGVaAVVIGIGINVNNTPPPEPLPDTSLATSLGKEVDRNELLEEL 162

                        170
                 ....*....|.
gi 505489704 178 AYELQKNLKIY 188
Cdd:cd16442  163 LAALENRLELF 173
birA_ligase TIGR00121
birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the ...
 20-252 4.90e-58

birA, biotin-[acetyl-CoA-carboxylase] ligase region; This model represents the biotin--acetyl-CoA-carboxylase ligase region of biotin--acetyl-CoA-carboxylase ligase. In Escherichia coli and some other species, this enzyme is part of a bifunction protein BirA that includes a small, N-terminal biotin operon repressor domain. Proteins identified by this model should not be called bifunctional unless they are also identified by birA_repr_reg (TIGR00122). The protein name suggests that this enzyme transfers biotin only to acetyl-CoA-carboxylase but it also transfers the biotin moiety to other proteins. The apparent orthologs among the eukaryotes are larger proteins that contain a single copy of this domain. [Protein fate, Protein modification and repair]


Pssm-ID: 272917 [Multi-domain]  Cd Length: 237  Bit Score: 184.53  E-value: 4.90e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   20 VFDEIDSTNEYLLKHYQTL-PQGSVCLAEQQTAGRGRRGRTWYSPQSeNVYFSILWHyPTEDAAHISSLSLVISLIIAES 98
Cdd:TIGR00121   4 VLDVIDSTNQYALELAKEGkLKGDLVVAEYQTAGRGRRGRKWLSPEG-GLYFSLILR-PDLPKSPAPGLTLVAGIAIAEV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   99 LQaQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGI-HLVIGIGLNLGMTKVDENIVAQAWSDLS--DYQFDRNALVC 175
Cdd:TIGR00121  82 LK-ELGDQVQVKWPNDILLKDKKLGGILTELTGKENRAdYVVIGIGINVQNRKPAESLREQAISLSEeaGIDLDRGELIE 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 505489704  176 RLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQNSVITPFGIGEMSLR 252
Cdd:TIGR00121 161 GFLRNFEENLEWFEQEGIDEILSKWEKLSAHIGREVSLTTGNGEIEGIARGIDKDGALLLEDGGGIKKIISGEISLR 237
PRK06955 PRK06955
biotin--[acetyl-CoA-carboxylase] ligase;
20-253 1.39e-37

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 235896 [Multi-domain]  Cd Length: 300  Bit Score: 133.75  E-value: 1.39e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  20 VFDEIDSTNEYLLKHYQTLP-------QGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHYPTEDAAhISSLSLVIS 92
Cdd:PRK06955  37 IVEETGSTNADLMARLKALPrsadalpAPIVRVAYEQTAGRGRQGRPWFAQPGNALLFSVACVLPRPVAA-LAGLSLAVG 115

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  93 LIIAESLQAQQV---ENIQIKWPNDIYYCGKKMGGILIET---KADRSGIhlVIGIGLNL-----GMTKVD--------- 152
Cdd:PRK06955 116 VALAEALAALPAalgQRIALKWPNDLLIAGRKLAGILIETvwaTPDATAV--VIGIGLNVrradaVAAEVDalrareaal 193

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 153 -------ENIVAQAWSDLSDYqfdRNALVCRLAYELQKnlkiYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEI-HGIS 224
Cdd:PRK06955 194 arglppvALAAACAGANLTDT---LAAALNALAPALQA----FGADGLAPFAARWHALHAYAGREVVLLEDGAELaRGVA 266

                        250       260       270
                 ....*....|....*....|....*....|....
gi 505489704 225 RGINQHGELLLEqnsviTPFGI-----GEMSLRS 253
Cdd:PRK06955 267 HGIDETGQLLLD-----TPAGRqaiaaGDVSLRE 295
BirA COG1654
Biotin operon repressor [Transcription];
4-255 2.02e-32

Biotin operon repressor [Transcription];


Pssm-ID: 441260 [Multi-domain]  Cd Length: 324  Bit Score: 120.86  E-value: 2.02e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   4 LNQSQIQTAL----LCGQALVFDEIDSTNEYLLK-HYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHyPT 78
Cdd:COG1654   66 LDPEEIRAGLstkrLGREILYVISSTSTNLLALElAAQGGDAGTVVAAEQQRGGRGRRRRSWSSPGGGGLLYSLLLR-PP 144

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  79 EDAAHISSLSLVISLIIAESLQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDENIVAQ 158
Cdd:COG1654  145 IAPALLSLLLLAAAVAVAAALAEGGGLVKWKKWPNDLLKKGKKILGILEEEGGDADGVVIVVGGGGNNNNSNPEEEPQEL 224

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 159 AWSDLSDYQFDRNALVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGINQHGELLLEQN 238
Cdd:COG1654  225 AELATSLLLILRLRLLRLLLLLLLLLLELLELLGFLEFFFLWERLDWELLRVLKLVVVVVEIGGGGGGGGALGGGLLGLL 304

                        250
                 ....*....|....*..
gi 505489704 239 SVITPFGIGEMSLRSDE 255
Cdd:COG1654  305 LLGGGGGGGEGSLSAVV 321
PRK08330 PRK08330
biotin--protein ligase; Provisional
 21-236 6.88e-29

biotin--protein ligase; Provisional


Pssm-ID: 169384 [Multi-domain]  Cd Length: 236  Bit Score: 109.45  E-value: 6.88e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  21 FDEIDSTNEYLLKHYQTLPQGSVCLAEQQTAGRGRRGRTWYSPQSeNVYFSILWHYPTEDaAHISSLSLVISLIIAESLQ 100
Cdd:PRK08330   8 FDEVDSTNEYAKRIAPDEEEGTVIVADRQTAGHGRKGRAWASPEG-GLWMSVILKPKVSP-EHLPKLVFLGALAVVDTLR 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 101 AQQVENiQIKWPNDIYYCGKKMGGILIETKADrsgiHLVIGIGLNLGmTKVDENIVAQAWS---------DLSDYqfdRN 171
Cdd:PRK08330  86 EFGIEG-KIKWPNDVLVNYKKIAGVLVEGKGD----FVVLGIGLNVN-NEIPDELRETATSmkevlgrevPLIEV---FK 156

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 505489704 172 ALVCRLAYELQKNLKIYPLVgfehYAERWQAFDIFRdRPVKLVTEGSEI-HGISRGINQHGELLLE 236
Cdd:PRK08330 157 RLVENLDRWYKLFLEGPGEI----LEEVKGRSMILG-KRVKIIGDGEILvEGIAEDIDEFGALILR 217
BPL_LplA_LipB pfam03099
Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, ...
 20-145 2.44e-23

Biotin/lipoate A/B protein ligase family; This family includes biotin protein ligase, lipoate-protein ligase A and B. Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Each organizm probably has only one BPL. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LPLA) catalyzes the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes. The unusual biosynthesis pathway of lipoic acid is mechanistically intertwined with attachment of the cofactor.


Pssm-ID: 427135  Cd Length: 132  Bit Score: 91.73  E-value: 2.44e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   20 VFDEIDSTNEYLLKHYQTLPQ-GSVCLAEQQTAGRGRRGRTWYSPQSEnVYFSILW--HYPTEDAAHISSLSLVISLIIA 96
Cdd:pfam03099   1 LGERIKSTNTYLEELNSSELEsGGVVVVRRQTGGRGRGGNVWHSPKGC-LTYSLLLskEHPNVDPSVLEFYVLELVLAVL 79

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 505489704   97 ESL--QAQQVENI--QIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLN 145
Cdd:pfam03099  80 EALglYKPGISGIpcFVKWPNDLYVNGRKLAGILQRSTRGGTLHHGVIGLGVN 132
PRK13325 PRK13325
bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;
6-254 3.49e-20

bifunctional biotin--[acetyl-CoA-carboxylase] ligase/type III pantothenate kinase;


Pssm-ID: 183976 [Multi-domain]  Cd Length: 592  Bit Score: 89.38  E-value: 3.49e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704   6 QSQIQTALLcgqalvfDEIDSTNEYLLKHYQTLP---QGSVCLAEQQTAGRGRRGRTWYSPQSENVYFSILWHYpTEDAA 82
Cdd:PRK13325  79 RSGFQTALK-------HECASSNDEILELARIAPdkaHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVF-DRPQY 150

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  83 HISSLSLVISLIIAESLQAQQVENiQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDENIVA-QAWS 161
Cdd:PRK13325 151 ELGSLSPVAAVACRRALSRLGLKT-QIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKEVENAASvQSLF 229

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 162 DLSDYQFDRNA--LVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEI-HGISRGINQHGELLLE-- 236
Cdd:PRK13325 230 QTASRRGNADAavLLETLLAELDAVLLQYARDGFAPFVAEYQAANRDHGKAVLLLRDGETVfEGTVKGVDGQGVLHLEta 309

                        250       260
                 ....*....|....*....|
gi 505489704 237 --QNSVITpfgiGEMSLRSD 254
Cdd:PRK13325 310 egKQTVVS----GEISLRSD 325
PTZ00276 PTZ00276
biotin/lipoate protein ligase; Provisional
 45-149 6.08e-19

biotin/lipoate protein ligase; Provisional


Pssm-ID: 140302 [Multi-domain]  Cd Length: 245  Bit Score: 82.99  E-value: 6.08e-19
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  45 LAEQQTAGRGRRGRTWYSPQSeNVYFSILWHYPTEDAAHISSLSLVISLI----IAESLQAQQVeniQIKWPNDIYYCGK 120
Cdd:PTZ00276  37 LAESQTAGRGTGGRTWTSPKG-NMYFTLCIPQKGVPPELVPVLPLITGLAcraaIMEVLHGAAV---HTKWPNDIIYAGK 112

                         90       100
                 ....*....|....*....|....*....
gi 505489704 121 KMGGILIETKADrsgiHLVIGIGLNLGMT 149
Cdd:PTZ00276 113 KIGGSLIESEGE----YLIIGIGMNIEVA 137
PRK08477 PRK08477
biotin--[acetyl-CoA-carboxylase] ligase;
19-185 9.57e-18

biotin--[acetyl-CoA-carboxylase] ligase;


Pssm-ID: 236273 [Multi-domain]  Cd Length: 211  Bit Score: 78.84  E-value: 9.57e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  19 LVFDEIDSTNEYLLKHYQTLP-QGSVCL-AEQQTAGRGRRGRTWYSPQSeNVYFSILwhYPTEDAAH---ISSLSLVISL 93
Cdd:PRK08477   4 RVFESLDSTQTYLIEKIKNGElKAPFAIvAKEQTAGIGSRGNSWEGKKG-NLFFSFA--LKESDLPKdlpLQSSSIYFGF 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  94 IIAESLQaQQVENIQIKWPNDIYYCGKKMGGILIETKADrsgiHLVIGIGLNLgmtkvdeNIVAQAWSDLsDYQFDRNAL 173
Cdd:PRK08477  81 LLKEVLK-ELGSKVWLKWPNDLYLDDKKIGGVITNKIKN----FIVCGIGLNL-------KFSPKNFACL-DIEISDDLL 147

                        170
                 ....*....|..
gi 505489704 174 VCRLAYELQKNL 185
Cdd:PRK08477 148 LEGFLQKIEKKI 159
PRK05935 PRK05935
biotin--protein ligase; Provisional
 23-185 1.01e-14

biotin--protein ligase; Provisional


Pssm-ID: 235649 [Multi-domain]  Cd Length: 190  Bit Score: 70.23  E-value: 1.01e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  23 EIDSTNEyLLKHYQTL--PQG-SVCLAEQQTAGRGRRGRTWYSpqSENVYFSILWHYPTEDAAHISSL-----SLVISLI 94
Cdd:PRK05935  10 ETPSTNT-TAKEGMHLwdPYAlTVISTREQTAGKGKFGKSWHS--SDQDLLASFCFFITVLNIDVSLLfrlgtEAVMRLG 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  95 iaeslQAQQVENIQIKWPNDIYYCGKKMGGILIETKADRSGIHLVIGIGLNLGMTKVDENIVAQAWSDLS-------DYQ 167
Cdd:PRK05935  87 -----EDLGITEAVIKWPNDVLVHGEKLCGVLCETIPVKGGLGVILGIGVNGNTTKDELLGIDQPATSLQellghpiDLE 161

                        170
                 ....*....|....*...
gi 505489704 168 FDRNALVCRLAYELQKNL 185
Cdd:PRK05935 162 EQRERLIKHIKHVLIQTL 179
PTZ00275 PTZ00275
biotin-acetyl-CoA-carboxylase ligase; Provisional
 19-241 4.03e-07

biotin-acetyl-CoA-carboxylase ligase; Provisional


Pssm-ID: 185536 [Multi-domain]  Cd Length: 285  Bit Score: 49.82  E-value: 4.03e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  19 LVFDEIDSTNEYLLKHYQTLPQGS--------VCLAEQQTAGRGRRG------RTWYSPQSeNVYFSILWHYPTEDAAHI 84
Cdd:PTZ00275  20 LHFDVLDSTQLYCKRNMKRFIQNGklqddnmiIVSCNEQTNGIGTRDtkknqdRIWLSEKG-NLFTTFVFLWNRNDIEKV 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  85 SSLSLVISLIIAESLQAQQVENiQIKWPNDIYYCGKKMGGILIE-------TKADRSGIHLVIGIGLNLGMTKvDENIVA 157
Cdd:PTZ00275  99 KYLAQTCTVAISKTLEYFHLVT-QIKWINDVLVNYKKIAGCLVHlyylddfPNLNSRYVCVMVGIGINVTLED-KHNLLN 176

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704 158 QAWSDL-SDYQFDRNA---------LVCRLAYELQKNLKIYPLVGFEHYAERWQAFDIFRDRPVKLVTEGSEIHGISRGI 227
Cdd:PTZ00275 177 NNYTSIkKELQRDFNTpksipsveqVTEKLIINLKAVINKLRKEGFSSFLDYITPRLLYKDKKVLIDQDNELIVGYLQGL 256

                        250
                 ....*....|....*.
gi 505489704 228 NQHGELLL--EQNSVI 241
Cdd:PTZ00275 257 LHDGSLLLlrEKNKLV 272
BPL_C pfam02237
Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It ...
 209-252 7.84e-04

Biotin protein ligase C terminal domain; The function of this structural domain is unknown. It is found to the C terminus of the biotin protein ligase catalytic domain pfam01317.


Pssm-ID: 426672 [Multi-domain]  Cd Length: 48  Bit Score: 36.29  E-value: 7.84e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 505489704  209 RPVKLVTEGSEIHGISRGINQHGELLLEQNSVITPFGI-GEMSLR 252
Cdd:pfam02237   4 REVRVLLGDGIVEGIAVGIDDDGALLLETDDGTIRDINsGEVSLR 48
BPL_LplA_LipB cd16435
biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), ...
 53-146 6.82e-03

biotin-lipoate ligase family; This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.


Pssm-ID: 319740  Cd Length: 198  Bit Score: 36.75  E-value: 6.82e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 505489704  53 RGRRGR-TWYSPQSENVYFSIlwhyPTEDAAHISSLSLVISLIIAESLQAQQVEnIQIKW-PNDIYYCGKKMGGILIETK 130
Cdd:cd16435   68 RNRGGRaVSHDPGQLVFSPVI----GPNVEFMISKFNLIIEEGIRDAIADFGQS-AEVKWgRNDLWIDNRKVCGIAVRVV 142

                         90
                 ....*....|....*.
gi 505489704 131 ADRsGIHlviGIGLNL 146
Cdd:cd16435  143 KEA-IFH---GIALNL 154
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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