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Conserved domains on  [gi|644349596|ref|WP_025301637|]
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MULTISPECIES: glutamate--cysteine ligase [Serratia]

Protein Classification

glutamate--cysteine ligase( domain architecture ID 10006790)

glutamate--cysteine ligase catalyzes the conversion of ATP, L-glutamate, and L-cysteine to ADP, phosphate, and gamma-L-glutamyl-L-cysteine

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
1-516 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


:

Pssm-ID: 442162  Cd Length: 518  Bit Score: 995.40  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   1 MIPDVSQALSWLEA--HPQALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDH 78
Cdd:COG2918    1 MSPDLSRRLAWLAApaHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  79 LLTFLRDIHRYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLE 158
Cdd:COG2918   81 LLAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEA 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 159 FWQAWAGVQ-DAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETtlPFERTEQGMCYLPYATSLRLS 237
Cdd:COG2918  161 FWQALQGLEgDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMS 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 238 DLGYTNKSQSNLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSD 317
Cdd:COG2918  239 DLGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIGVKVDGEYRQLNTNILQIENEYYSPIRPKRVTRSGEKPSQ 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 318 ALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCGA 397
Cdd:COG2918  319 ALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQRGGEE 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 398 shEPLENVGKALFADLRRVAEVLDGEAGNRQYQQVCDELVAAFDDPELTFSARILKAMKAEGTG--RVGLQLAEQYRQTL 475
Cdd:COG2918  399 --IPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGfgEFGLRLAKQHREYL 476
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 644349596 476 LEEPLEILNEAELDKEREASWQRQRDIEANDTLSFEAFLKQ 516
Cdd:COG2918  477 RAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLAR 517
 
Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
1-516 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


Pssm-ID: 442162  Cd Length: 518  Bit Score: 995.40  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   1 MIPDVSQALSWLEA--HPQALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDH 78
Cdd:COG2918    1 MSPDLSRRLAWLAApaHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  79 LLTFLRDIHRYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLE 158
Cdd:COG2918   81 LLAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEA 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 159 FWQAWAGVQ-DAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETtlPFERTEQGMCYLPYATSLRLS 237
Cdd:COG2918  161 FWQALQGLEgDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMS 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 238 DLGYTNKSQSNLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSD 317
Cdd:COG2918  239 DLGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIGVKVDGEYRQLNTNILQIENEYYSPIRPKRVTRSGEKPSQ 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 318 ALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCGA 397
Cdd:COG2918  319 ALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQRGGEE 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 398 shEPLENVGKALFADLRRVAEVLDGEAGNRQYQQVCDELVAAFDDPELTFSARILKAMKAEGTG--RVGLQLAEQYRQTL 475
Cdd:COG2918  399 --IPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGfgEFGLRLAKQHREYL 476
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 644349596 476 LEEPLEILNEAELDKEREASWQRQRDIEANDTLSFEAFLKQ 516
Cdd:COG2918  477 RAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLAR 517
glu_cys_ligase TIGR01434
glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model ...
6-515 0e+00

glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model represents glutamate--cysteine ligase, and enzyme in the biosynthesis of glutathione (GSH). GSH is one of several low molecular weight cysteine derivatives that can serve to protect against oxidative damage and participate in a biosynthetic or detoxification reactions. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]


Pssm-ID: 213621  Cd Length: 512  Bit Score: 883.82  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596    6 SQALSWLEAHPQALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRD 85
Cdd:TIGR01434   1 SQALAWLEKNPQALKGIQRGIERETLRVNADGTLATTGHPEALGSALTHKWITTDFSEALLEFITPTDGDIAHMLTFLRD 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   86 IHRYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAG 165
Cdd:TIGR01434  81 LHRFTASKLGDERMWPLSMPCYIADGQDIEIAQYGTSNIGRFKTLYREGLKNRYGALMQTISGIHYNFSLPMAFWQAKRG 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  166 VQDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETTLPFERTEQGMCYLPYATSLRLSDLGYTNKS 245
Cdd:TIGR01434 161 AEGGESAKDKISAGYFRLIRNYYRFGWVIPYLFGASPAISSSFLQGKPTKLPFEKTDEGMYYLPYATSLRLSDLGYTNKS 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  246 QSNLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALLRGGIE 325
Cdd:TIGR01434 241 QSNLGITFNDLQEYIASLKQAIKTPSEEYAKIGTKKDGKRLQLNTNILQIENELYAPIRPKRVTRSGESPSDALLRRGIE 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  326 YIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCGASHEPLENV 405
Cdd:TIGR01434 321 YVEVRSLDINPFSPIGIDEQQVRFLDLFLVWCALADSPEMSSGELLCTTDNWNRVILEGRKPGLTLGIGCETAQFPLPVV 400
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  406 GKALFADLRRVAEVLDGEAGNRQYQQVCDELVAAFDDPELTFSARILKAMKAEGTGRVGLQLAEQYRQTLLEEPLEILNE 485
Cdd:TIGR01434 401 GKDLFRDLKRVAQTLDSIRGGEAYQKACDELVACFDNPELTFSAQILRSMIDTGIGGTGLAFAEAYRELFREEPLEILEQ 480
                         490       500       510
                  ....*....|....*....|....*....|
gi 644349596  486 AELDKEREASWQRQRDIEANDTLSFEAFLK 515
Cdd:TIGR01434 481 ERFEAERSASLRRQQEIERADTEPFATWLA 510
Glu_cys_ligase pfam04262
Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that ...
10-380 0e+00

Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that carry out the first step of the glutathione biosynthesis pathway.


Pssm-ID: 427826  Cd Length: 371  Bit Score: 725.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   10 SWLEAHPQ--ALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRDIH 87
Cdd:pfam04262   1 ALLSDNPHlpLLKGILRGIERESLRVTPDGKLAQTPHPKALGSALTHPWITTDFSESLLEFITPPFSDVDETLDFLNDLH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   88 RYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVQ 167
Cdd:pfam04262  81 RFVYRNLGDELLWPLSMPCVLPDEEDIPIAQYGTSNIGRMKTVYREGLAHRYGKKMQTIAGVHYNFSLPDSFWPLLQELE 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  168 DAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGREttLPFERTEQGMCYLPYATSLRLSDLGYTNKSQS 247
Cdd:pfam04262 161 GSELSQDFISDGYFGLIRNFYRYGWLLPYLFGASPALCSSFLKGRN--HPLEKLDAGTLYLPYATSLRMSDLGYTNKAQA 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  248 NLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALLRGGIEYI 327
Cdd:pfam04262 239 SLKISYNSLDEYVKSLKKAISTPSPEYEKIGVKVDGEYRQLNTNVLQIENEFYSPIRPKRVTKSGERPLDALLRRGVEYI 318
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|...
gi 644349596  328 EVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRV 380
Cdd:pfam04262 319 EVRSLDINPFSPLGIDEEQLRFLDLFLLYCLLEESPEIDEEELAEIRKNQERV 371
PRK02471 PRK02471
bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;
7-488 3.57e-101

bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;


Pssm-ID: 179427 [Multi-domain]  Cd Length: 752  Bit Score: 320.72  E-value: 3.57e-101
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   7 QALSWLEAHPQALKGiRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRDI 86
Cdd:PRK02471   5 KLIQKLHLRPLLLQA-NFGLEKESLRVDSDGKLALTPHPKAFGSRSFHPYIQTDFSESQLELITPVADSIEEVYRFLGAL 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  87 HRYVARNIG-DERMWPLSMPCFIEAEQDIELAQFGssniGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQA-WA 164
Cdd:PRK02471  84 HDVASRSLDeDEYLWPLSMPPILPDEEEIPIAQLD----NREDVQYREYLAKKYGKKKQMISGIHYNFSLGEALIQRlFE 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 165 GVQDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKgrettlpferteQGMCYL-PYATSLRLSDLGYTN 243
Cdd:PRK02471 160 EQSEQQDYKDFKNDLYLKVARNFLRYRWLLTYLFGASPVAEADFFK------------QNKEYLnQDVRSLRNSKYGYVN 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 244 KSQsnLGITFNDLHSYVAGLKRAIvtpseefaklgvkegdrylqlNSNVLQIENELYAPIRPKrvtksGETPSDALLRGG 323
Cdd:PRK02471 228 KED--VYVSYNSLEDYVSSLENAV---------------------ESGQLISEKEFYSPVRLR-----GAKQDRSLLEKG 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 324 IEYIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGrkPGQTIgigcgashePLE 403
Cdd:PRK02471 280 ITYLEFRLFDLNPFEPYGISQETLDFVHLFLLYLLWLDEPEDTDQWVELADENNNLIALEH--PLEAL---------ALA 348
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 404 NVGKALFADLRRVAEVLDGEAGnrqYQQVCDELVAAFDDPELTFSARILKAMKAEGTGRVGLQLAEQYRQTLLEEP--LE 481
Cdd:PRK02471 349 AEAELILDAMEDLVQHLGLPDE---YQDLIKQLKEQLTDPEKTLSGRLLKQIKQESYVAFGLEQAKQYKEYAWERPyaLK 425

                 ....*..
gi 644349596 482 ILNEAEL 488
Cdd:PRK02471 426 GYEDMEL 432
 
Name Accession Description Interval E-value
GshA COG2918
Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];
1-516 0e+00

Gamma-glutamylcysteine synthetase [Coenzyme transport and metabolism];


Pssm-ID: 442162  Cd Length: 518  Bit Score: 995.40  E-value: 0e+00
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   1 MIPDVSQALSWLEA--HPQALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDH 78
Cdd:COG2918    1 MSPDLSRRLAWLAApaHPSLLKGILRGIERETLRVTPDGRLAQTPHPAALGSALTHPWITTDYSEALLEFITPVFDDIDE 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  79 LLTFLRDIHRYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLE 158
Cdd:COG2918   81 LLAFLRDIHRFVYRHLGDELLWPLSMPCFLPGEEDIPIAQYGSSNIGRMKTVYRRGLKNRYGRLMQTIAGVHYNFSLPEA 160
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 159 FWQAWAGVQ-DAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETtlPFERTEQGMCYLPYATSLRLS 237
Cdd:COG2918  161 FWQALQGLEgDSESLQDFISAGYFGLIRNFRRFGWLIPYLFGASPAVCKSFLKGREH--PLEEFDKGTLYLPYATSLRMS 238
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 238 DLGYTNKSQSNLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSD 317
Cdd:COG2918  239 DLGYQNKAQSGLKICYNSLDSYVDSLRRAIRTPSPEYEKIGVKVDGEYRQLNTNILQIENEYYSPIRPKRVTRSGEKPSQ 318
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 318 ALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCGA 397
Cdd:COG2918  319 ALLRRGVEYIEVRSLDINPFSPIGIDEEQIRFLDLFLLWCLLADSPPISAEELAEIDDNFNRVVLEGRKPGLTLQRGGEE 398
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 398 shEPLENVGKALFADLRRVAEVLDGEAGNRQYQQVCDELVAAFDDPELTFSARILKAMKAEGTG--RVGLQLAEQYRQTL 475
Cdd:COG2918  399 --IPLQDWGKELLDDLQPVAELLDAAHGGGAYQAALAAQRAKLDDPELTPSARILAEMRENGEGfgEFGLRLAKQHREYL 476
                        490       500       510       520
                 ....*....|....*....|....*....|....*....|.
gi 644349596 476 LEEPLEILNEAELDKEREASWQRQRDIEANDTLSFEAFLKQ 516
Cdd:COG2918  477 RAEPLSAETEAEFEAEAEESLAEQAEIEAADTLSFDEFLAR 517
glu_cys_ligase TIGR01434
glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model ...
6-515 0e+00

glutamate--cysteine ligase; Alternate name: gamma-glutamylcysteine synthetase. This model represents glutamate--cysteine ligase, and enzyme in the biosynthesis of glutathione (GSH). GSH is one of several low molecular weight cysteine derivatives that can serve to protect against oxidative damage and participate in a biosynthetic or detoxification reactions. [Biosynthesis of cofactors, prosthetic groups, and carriers, Glutathione and analogs]


Pssm-ID: 213621  Cd Length: 512  Bit Score: 883.82  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596    6 SQALSWLEAHPQALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRD 85
Cdd:TIGR01434   1 SQALAWLEKNPQALKGIQRGIERETLRVNADGTLATTGHPEALGSALTHKWITTDFSEALLEFITPTDGDIAHMLTFLRD 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   86 IHRYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAG 165
Cdd:TIGR01434  81 LHRFTASKLGDERMWPLSMPCYIADGQDIEIAQYGTSNIGRFKTLYREGLKNRYGALMQTISGIHYNFSLPMAFWQAKRG 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  166 VQDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGRETTLPFERTEQGMCYLPYATSLRLSDLGYTNKS 245
Cdd:TIGR01434 161 AEGGESAKDKISAGYFRLIRNYYRFGWVIPYLFGASPAISSSFLQGKPTKLPFEKTDEGMYYLPYATSLRLSDLGYTNKS 240
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  246 QSNLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALLRGGIE 325
Cdd:TIGR01434 241 QSNLGITFNDLQEYIASLKQAIKTPSEEYAKIGTKKDGKRLQLNTNILQIENELYAPIRPKRVTRSGESPSDALLRRGIE 320
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  326 YIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGRKPGQTIGIGCGASHEPLENV 405
Cdd:TIGR01434 321 YVEVRSLDINPFSPIGIDEQQVRFLDLFLVWCALADSPEMSSGELLCTTDNWNRVILEGRKPGLTLGIGCETAQFPLPVV 400
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  406 GKALFADLRRVAEVLDGEAGNRQYQQVCDELVAAFDDPELTFSARILKAMKAEGTGRVGLQLAEQYRQTLLEEPLEILNE 485
Cdd:TIGR01434 401 GKDLFRDLKRVAQTLDSIRGGEAYQKACDELVACFDNPELTFSAQILRSMIDTGIGGTGLAFAEAYRELFREEPLEILEQ 480
                         490       500       510
                  ....*....|....*....|....*....|
gi 644349596  486 AELDKEREASWQRQRDIEANDTLSFEAFLK 515
Cdd:TIGR01434 481 ERFEAERSASLRRQQEIERADTEPFATWLA 510
Glu_cys_ligase pfam04262
Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that ...
10-380 0e+00

Glutamate-cysteine ligase; Family of bacterial f glutamate-cysteine ligases (EC:6.3.2.2) that carry out the first step of the glutathione biosynthesis pathway.


Pssm-ID: 427826  Cd Length: 371  Bit Score: 725.09  E-value: 0e+00
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   10 SWLEAHPQ--ALKGIRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRDIH 87
Cdd:pfam04262   1 ALLSDNPHlpLLKGILRGIERESLRVTPDGKLAQTPHPKALGSALTHPWITTDFSESLLEFITPPFSDVDETLDFLNDLH 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   88 RYVARNIGDERMWPLSMPCFIEAEQDIELAQFGSSNIGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVQ 167
Cdd:pfam04262  81 RFVYRNLGDELLWPLSMPCVLPDEEDIPIAQYGTSNIGRMKTVYREGLAHRYGKKMQTIAGVHYNFSLPDSFWPLLQELE 160
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  168 DAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKGREttLPFERTEQGMCYLPYATSLRLSDLGYTNKSQS 247
Cdd:pfam04262 161 GSELSQDFISDGYFGLIRNFYRYGWLLPYLFGASPALCSSFLKGRN--HPLEKLDAGTLYLPYATSLRMSDLGYTNKAQA 238
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  248 NLGITFNDLHSYVAGLKRAIVTPSEEFAKLGVKEGDRYLQLNSNVLQIENELYAPIRPKRVTKSGETPSDALLRGGIEYI 327
Cdd:pfam04262 239 SLKISYNSLDEYVKSLKKAISTPSPEYEKIGVKVDGEYRQLNTNVLQIENEFYSPIRPKRVTKSGERPLDALLRRGVEYI 318
                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|...
gi 644349596  328 EVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRV 380
Cdd:pfam04262 319 EVRSLDINPFSPLGIDEEQLRFLDLFLLYCLLEESPEIDEEELAEIRKNQERV 371
PRK02471 PRK02471
bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;
7-488 3.57e-101

bifunctional glutamate--cysteine ligase GshA/glutathione synthetase GshB;


Pssm-ID: 179427 [Multi-domain]  Cd Length: 752  Bit Score: 320.72  E-value: 3.57e-101
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596   7 QALSWLEAHPQALKGiRRGIERETLRVTPNGTLATTGHPEKLGAALTHHWITTDFAEALLEFITPVDDNIDHLLTFLRDI 86
Cdd:PRK02471   5 KLIQKLHLRPLLLQA-NFGLEKESLRVDSDGKLALTPHPKAFGSRSFHPYIQTDFSESQLELITPVADSIEEVYRFLGAL 83
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596  87 HRYVARNIG-DERMWPLSMPCFIEAEQDIELAQFGssniGRMKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQA-WA 164
Cdd:PRK02471  84 HDVASRSLDeDEYLWPLSMPPILPDEEEIPIAQLD----NREDVQYREYLAKKYGKKKQMISGIHYNFSLGEALIQRlFE 159
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 165 GVQDAESGKEQISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLKgrettlpferteQGMCYL-PYATSLRLSDLGYTN 243
Cdd:PRK02471 160 EQSEQQDYKDFKNDLYLKVARNFLRYRWLLTYLFGASPVAEADFFK------------QNKEYLnQDVRSLRNSKYGYVN 227
                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 244 KSQsnLGITFNDLHSYVAGLKRAIvtpseefaklgvkegdrylqlNSNVLQIENELYAPIRPKrvtksGETPSDALLRGG 323
Cdd:PRK02471 228 KED--VYVSYNSLEDYVSSLENAV---------------------ESGQLISEKEFYSPVRLR-----GAKQDRSLLEKG 279
                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 324 IEYIEVRSLDINPFSPIGVDAVQARFLDLFLVWCALADAPEMSSDELLCTRKNWNRVILEGrkPGQTIgigcgashePLE 403
Cdd:PRK02471 280 ITYLEFRLFDLNPFEPYGISQETLDFVHLFLLYLLWLDEPEDTDQWVELADENNNLIALEH--PLEAL---------ALA 348
                        410       420       430       440       450       460       470       480
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 644349596 404 NVGKALFADLRRVAEVLDGEAGnrqYQQVCDELVAAFDDPELTFSARILKAMKAEGTGRVGLQLAEQYRQTLLEEP--LE 481
Cdd:PRK02471 349 AEAELILDAMEDLVQHLGLPDE---YQDLIKQLKEQLTDPEKTLSGRLLKQIKQESYVAFGLEQAKQYKEYAWERPyaLK 425

                 ....*..
gi 644349596 482 ILNEAEL 488
Cdd:PRK02471 426 GYEDMEL 432
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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