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Conserved domains on  [gi|759621471|ref|WP_043339529|]
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MULTISPECIES: transcription termination/antitermination protein NusG [Belnapia]

Protein Classification

transcription termination/antitermination protein NusG( domain architecture ID 11489978)

transcription termination/antitermination protein NusG (N-Utilization Substance G) is involved in transcription elongation and termination in bacteria

CATH:  2.30.30.30|3.30.70.940
Gene Ontology:  GO:0006353|GO:0032784|GO:0031564|GO:0006354
PubMed:  27899640|29887376
SCOP:  4002609

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 5-176 1.71e-80

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


:

Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 236.04  E-value: 1.71e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    5 WYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   85 VTGFLGNKNKPSPITE-AEAQRIVNQVKEGVEKPrKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIFGR 163
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEdEEVKNILNALEEGKDKP-KPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGR 159

                         170
                  ....*....|...
gi 759621471  164 STPVELEYTQVEK 176
Cdd:TIGR00922 160 ETPVELEFSQVEK 172
 
Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 5-176 1.71e-80

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 236.04  E-value: 1.71e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    5 WYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   85 VTGFLGNKNKPSPITE-AEAQRIVNQVKEGVEKPrKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIFGR 163
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEdEEVKNILNALEEGKDKP-KPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGR 159

                         170
                  ....*....|...
gi 759621471  164 STPVELEYTQVEK 176
Cdd:TIGR00922 160 ETPVELEFSQVEK 172
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
1-176 4.16e-78

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 230.10  E-value: 4.16e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   1 MAKRWYVVHVYSGFEKKIAQQIKEQAaqKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVK 80
Cdd:COG0250    3 MEKRWYVVHTYSGYEKKVKENLERRI--EGI-----EVFVPTEEVVEIKNGKKKTVERPLFPGYVFVRMDLTDESWYLVR 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  81 DTPKVTGFLGNKNKPSPITEAEAQRIVNQVKEGVEKPRkPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSI 160
Cdd:COG0250   76 NTPGVTGFVGFGGKPAPLPDEEVERILARLEEGEEKPR-PKVDFEVGDRVRITDGPFAGFEGTVEEVDPEKGRVKVLVSI 154

                        170
                 ....*....|....*.
gi 759621471 161 FGRSTPVELEYTQVEK 176
Cdd:COG0250  155 FGRETPVELDFSQVEK 170
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 4-110 5.04e-55

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 169.19  E-value: 5.04e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVEVKNGKKKVKERKLFPGYVLVEMDMNDDTWHLVRNTP 80

                         90       100
                 ....*....|....*....|....*..
gi 759621471  84 KVTGFLGNKNKPSPITEAEAQRIVNQV 110
Cdd:cd09891   81 GVTGFVGSGGKPVPLSEEEVERILGQV 107
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 4-109 1.02e-38

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 127.87  E-value: 1.02e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471     4 RWYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPTEEVKEIRRGKKKVVERKLFPGYIFVEADLEDEVWTAIRGTP 80

                           90       100
                   ....*....|....*....|....*.
gi 759621471    84 KVTGFLGNKNKPSPITEAEAQRIVNQ 109
Cdd:smart00738  81 GVRGFVGGGGKPTPVPDDEIEKILKP 106
NusG pfam02357
Transcription termination factor nusG;
3-106 2.42e-38

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 126.57  E-value: 2.42e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    3 KRWYVVHVYSGFEKKIAQQIKEQaaqkGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDT 82
Cdd:pfam02357   1 KKWYVLQTYSGKEKKVKENLERQ----GI-----EVFLPTEEVVEIRNGKKKVVERPLFPGYVFVRMDMTDETWHLVRST 71

                          90       100
                  ....*....|....*....|....
gi 759621471   83 PKVTGFLGNKNKPSPITEAEAQRI 106
Cdd:pfam02357  72 PGVTGFVGGSGKPTPIPDEEVERI 95
antiterm_LoaP NF033641
antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The ...
 5-176 3.12e-27

antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The founding member, from Bacillus amyloliquefaciens, was shown to serve as an antiterminator for the transcription of genes involved in antibiotic biosynthesis.


Pssm-ID: 468124 [Multi-domain]  Cd Length: 166  Bit Score: 100.54  E-value: 3.12e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIkeqaaQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:NF033641   1 WYVLFVRTGKEEKVKKLL-----NKLFDKELLRCFVPKRKLPERKQGKWYKVIKPLFPGYVFIETDMNEELYYKLKKIPG 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  85 VTGFLGNKNK-PSPITEAEAQRIVNQVKEG--VEkprkPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIF 161
Cdd:NF033641  76 IIRLLGNEGDeFSPIPEEEMEFLLKLLNDDdiIE----YSKGYIEGDKVIVISGPLKGLEGIIKKIDKRKRRAKIELNFM 151

                        170
                 ....*....|....*
gi 759621471 162 GRSTPVELEYTQVEK 176
Cdd:NF033641 152 GEERLVDVGIEIVSK 166
antiterm_UpxY NF033644
UpxY family transcription antiterminator; The UpxY family of NusG-related transcription ...
 5-160 1.53e-13

UpxY family transcription antiterminator; The UpxY family of NusG-related transcription antiterminators was described originally from a paralogous family of eight members from Bacteriodes fragilis, UpaY to UphY, each of which was associated with a distinct capsular polysaccharide biosynthesis locus. There is no UpxY protein per se.


Pssm-ID: 468125 [Multi-domain]  Cd Length: 162  Bit Score: 64.80  E-value: 1.53e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIKEqaaqKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSdDAWHLVKDTPK 84
Cdd:NF033644   1 WYALYTRPRREKKVAELLEK----KGI-----ESFLPMQKEIRQWSDRKKRVEVPLIPNLVFVHITEK-ELDEVLEQTPG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  85 VTGFL---GNKNKPSPITEAEAQRI---VNQVKEGVEkprKPAVVFEVGEQVRVADGPFTSFMGTVEEVdeeRGRVKVSV 158
Cdd:NF033644  71 VVRYIrddRGKSKPAIIPDKQMERFrlmLDPSDEVVV---YLEAPLKKGDKVRVIGGPLKGLEGELVRV---KGKKRVVV 144


                 ..
gi 759621471 159 SI 160
Cdd:NF033644 145 RI 146
nusG PRK08559
transcription antitermination protein NusG; Validated
 6-158 3.37e-05

transcription antitermination protein NusG; Validated


Pssm-ID: 181467 [Multi-domain]  Cd Length: 153  Bit Score: 41.78  E-value: 3.37e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   6 YVVHVYSGFEKKIAQQIKEQAAQKGLGdqFDEILVPaEEVTevrrgqkvnserkffpGYVLVKMEMSDDAWHLVKDTPKV 85
Cdd:PRK08559   9 FAVKTTAGQERNVALMLAMRAKKENLP--IYAILAP-PELK----------------GYVLVEAESKGAVEEAIRGIPHV 69

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 759621471  86 TGFLgnknkPSPITEAEAQRIVnqvkegveKPRKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSV 158
Cdd:PRK08559  70 RGVV-----PGEISFEEVEHFL--------KPKPIVEGIKEGDIVELIAGPFKGEKARVVRVDESKEEVTVEL 129
 
Name Accession Description Interval E-value
nusG TIGR00922
transcription termination/antitermination factor NusG; NusG proteins are transcription factors ...
 5-176 1.71e-80

transcription termination/antitermination factor NusG; NusG proteins are transcription factors which are aparrently universal in prokaryotes (archaea and eukaryotes have homologs that may have related functions). The essential components of these factors include an N-terminal RNP-like (ribonucleoprotein) domain and a C-terminal KOW motif (pfam00467) believed to be a nucleic acid binding domain. In E. coli, NusA has been shown to interact with RNA polymerase and termination factor Rho. This model covers a wide variety of bacterial species but excludes mycoplasmas which are covered by a separate model (TIGR01956).The function of all of these NusG proteins is likely to be the same at the level of interaction with RNA and other protein factors to affect termination; however different species may utilize NusG towards different processes and in combination with different suites of affector proteins.In E. coli, NusG promotes rho-dependent termination. It is an essential gene. In Streptomyces virginiae and related species, an additional N-terminal sequence is also present and is suggested to play a role in butyrolactone-mediated autoregulation. In Thermotoga maritima, NusG has a long insert, fails to substitute for E. coli NusG (with or without the long insert), is a large 0.7 % of total cellular protein, and has a general, sequence non-specific DNA and RNA binding activity that blocks ethidium staining, yet permits transcription.Archaeal proteins once termed NusG share the KOW domain but are actually a ribosomal protein corresponding to L24p in bacterial and L26e in eukaryotes (TIGR00405). [Transcription, Transcription factors]


Pssm-ID: 273341 [Multi-domain]  Cd Length: 172  Bit Score: 236.04  E-value: 1.71e-80
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    5 WYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:TIGR00922   1 WYVVQTYSGYEKKVKQNLEELIELLGMGDYIFEVIVPTEEVVEIKKGKKKVVERKIFPGYVLVKMDLTDVSWHLVKNTPG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   85 VTGFLGNKNKPSPITE-AEAQRIVNQVKEGVEKPrKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIFGR 163
Cdd:TIGR00922  81 VTGFVGSGGKPKALSEdEEVKNILNALEEGKDKP-KPKIDFEVGEQVRVNDGPFANFTGTVEEVDYEKSKLKVSVSIFGR 159

                         170
                  ....*....|...
gi 759621471  164 STPVELEYTQVEK 176
Cdd:TIGR00922 160 ETPVELEFSQVEK 172
NusG COG0250
Transcription termination/antitermination protein NusG [Transcription];
1-176 4.16e-78

Transcription termination/antitermination protein NusG [Transcription];


Pssm-ID: 440020 [Multi-domain]  Cd Length: 171  Bit Score: 230.10  E-value: 4.16e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   1 MAKRWYVVHVYSGFEKKIAQQIKEQAaqKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVK 80
Cdd:COG0250    3 MEKRWYVVHTYSGYEKKVKENLERRI--EGI-----EVFVPTEEVVEIKNGKKKTVERPLFPGYVFVRMDLTDESWYLVR 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  81 DTPKVTGFLGNKNKPSPITEAEAQRIVNQVKEGVEKPRkPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSI 160
Cdd:COG0250   76 NTPGVTGFVGFGGKPAPLPDEEVERILARLEEGEEKPR-PKVDFEVGDRVRITDGPFAGFEGTVEEVDPEKGRVKVLVSI 154

                        170
                 ....*....|....*.
gi 759621471 161 FGRSTPVELEYTQVEK 176
Cdd:COG0250  155 FGRETPVELDFSQVEK 170
NGN_Bact_1 cd09891
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The ...
 4-110 5.04e-55

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 1; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination in bacteria. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Homologs of the NusG gene exist in all bacteria. The NusG N-terminal domain (NGN) is similar in all NusG homologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG suggests that the common properties of NusG and its homologs are due to their similar NGN domains.


Pssm-ID: 193580 [Multi-domain]  Cd Length: 107  Bit Score: 169.19  E-value: 5.04e-55
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:cd09891    1 KWYVVHTYSGYENKVKENLEKRIESEGLEDYIGEVLVPTEEVVEVKNGKKKVKERKLFPGYVLVEMDMNDDTWHLVRNTP 80

                         90       100
                 ....*....|....*....|....*..
gi 759621471  84 KVTGFLGNKNKPSPITEAEAQRIVNQV 110
Cdd:cd09891   81 GVTGFVGSGGKPVPLSEEEVERILGQV 107
NGN smart00738
In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, ...
 4-109 1.02e-38

In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold; In Spt5p, this domain may confer affinity for Spt4p.Spt4p


Pssm-ID: 197850 [Multi-domain]  Cd Length: 106  Bit Score: 127.87  E-value: 1.02e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471     4 RWYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:smart00738   1 NWYAVRTTSGQEKRVAENLERKAEALGLEDKIVSILVPTEEVKEIRRGKKKVVERKLFPGYIFVEADLEDEVWTAIRGTP 80

                           90       100
                   ....*....|....*....|....*.
gi 759621471    84 KVTGFLGNKNKPSPITEAEAQRIVNQ 109
Cdd:smart00738  81 GVRGFVGGGGKPTPVPDDEIEKILKP 106
NusG pfam02357
Transcription termination factor nusG;
3-106 2.42e-38

Transcription termination factor nusG;


Pssm-ID: 426736 [Multi-domain]  Cd Length: 96  Bit Score: 126.57  E-value: 2.42e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    3 KRWYVVHVYSGFEKKIAQQIKEQaaqkGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDT 82
Cdd:pfam02357   1 KKWYVLQTYSGKEKKVKENLERQ----GI-----EVFLPTEEVVEIRNGKKKVVERPLFPGYVFVRMDMTDETWHLVRST 71

                          90       100
                  ....*....|....*....|....
gi 759621471   83 PKVTGFLGNKNKPSPITEAEAQRI 106
Cdd:pfam02357  72 PGVTGFVGGSGKPTPIPDEEVERI 95
antiterm_LoaP NF033641
antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The ...
 5-176 3.12e-27

antiterminator LoaP; LoaP is a paralog of NusG with an extensive presence in Firmicutes. The founding member, from Bacillus amyloliquefaciens, was shown to serve as an antiterminator for the transcription of genes involved in antibiotic biosynthesis.


Pssm-ID: 468124 [Multi-domain]  Cd Length: 166  Bit Score: 100.54  E-value: 3.12e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIkeqaaQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:NF033641   1 WYVLFVRTGKEEKVKKLL-----NKLFDKELLRCFVPKRKLPERKQGKWYKVIKPLFPGYVFIETDMNEELYYKLKKIPG 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  85 VTGFLGNKNK-PSPITEAEAQRIVNQVKEG--VEkprkPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIF 161
Cdd:NF033641  76 IIRLLGNEGDeFSPIPEEEMEFLLKLLNDDdiIE----YSKGYIEGDKVIVISGPLKGLEGIIKKIDKRKRRAKIELNFM 151

                        170
                 ....*....|....*
gi 759621471 162 GRSTPVELEYTQVEK 176
Cdd:NF033641 152 GEERLVDVGIEIVSK 166
KOW_NusG cd06091
NusG contains an NGN domain at its N-terminus and KOW motif at its C-terminus; KOW_NusG motif ...
 124-176 7.11e-25

NusG contains an NGN domain at its N-terminus and KOW motif at its C-terminus; KOW_NusG motif is one of the two domains of N-Utilization Substance G (NusG) a transcription elongation and Rho-termination factor in bacteria and archaea. KOW domain is known as an RNA-binding motif that is shared so far among some families of ribosomal proteins, the essential bacterial transcriptional elongation factor NusG, the eukaryotic chromatin elongation factor Spt5, the higher eukaryotic KIN17 proteins and Mtr4. The eukaryotic ortholog of NusG is Spt5 with multiple KOW motifs at its C-terminus.


Pssm-ID: 240515 [Multi-domain]  Cd Length: 56  Bit Score: 90.98  E-value: 7.11e-25
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 759621471 124 FEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIFGRSTPVELEYTQVEK 176
Cdd:cd06091    4 FEVGDTVRIISGPFAGFEGKVEEIDEEKGKVKVLVEMFGRETPVELDFDQVEK 56
NGN cd08000
N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization ...
 5-106 1.32e-22

N-Utilization Substance G (NusG) N-terminal (NGN) domain Superfamily; The N-Utilization Substance G (NusG) and its eukaryotic homolog Spt5 are involved in transcription elongation and termination. NusG contains an NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein composed of an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms a Spt4-Spt5 complex that is an essential RNA Polymerase II elongation factor. NusG was originally discovered as an N-dependent antitermination enhancing activity in Escherichia coli and has a variety of functions, such as being involved in RNA polymerase elongation and Rho-termination in bacteria. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The diverse activities suggest that, after diverging from a common ancestor, NusG proteins became specialized in different bacteria.


Pssm-ID: 193574 [Multi-domain]  Cd Length: 99  Bit Score: 86.60  E-value: 1.32e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIKEQAAQKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTPK 84
Cdd:cd08000    2 WYVLFVKTGREEKVEKLLEKRFEANDI-----EAFVPKKEVPERKRGKIEEVIKPLFPGYVFVETDLSPELYELIREVPG 76

                         90       100
                 ....*....|....*....|..
gi 759621471  85 VTGFLGNKNKPSPITEAEAQRI 106
Cdd:cd08000   77 VIGILGNGEEPSPVSDEEIEMI 98
NusG_myco TIGR01956
NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the ...
 4-176 1.22e-14

NusG family protein; This model represents a family of Mycoplasma proteins orthologous to the bacterial transcription termination/antitermination factor NusG. These sequences from Mycoplasma are notably diverged (long branches in a Neighbor-joining phylogenetic tree) from the bacterial species. And although NusA and ribosomal protein S10 (NusE) appear to be present, NusB may be absent in Mycoplasmas calling into question whether these species have a functional Nus system including this family as a member.


Pssm-ID: 273895 [Multi-domain]  Cd Length: 258  Bit Score: 69.61  E-value: 1.22e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    4 RWYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVN---------------------------- 55
Cdd:TIGR01956   1 QWYIATTINGNEDEVIENIKAKVRALGLENYISDFKILKEREIEEKVFEPKNgqaprsmkntattkwetldetkykktki 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   56 SERKFFPGYVLVKMEMSDDAWHLVKDTPKVTGFLGNKNK---PSPITEAEAQR------IVNQVKEGV------------ 114
Cdd:TIGR01956  81 SEKNKYNGYIYIKMIMTEDAWFLIRNTENVTGLVGSSGKgakPIPISADADKLkmlkgiSENTKKRVLvtntaivemeen 160

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  115 -----------EKPRKPAVV-------------------------FEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSV 158
Cdd:TIGR01956 161 kfdekcqyilkHKQVKPEAIaqvsesgeiideiveefqlvdnlskFRVGNFVKIVDGPFKGIVGKIKKIDQEKKKAIVEV 240

                         250
                  ....*....|....*...
gi 759621471  159 SIFGRSTPVELEYTQVEK 176
Cdd:TIGR01956 241 EILGKSVDVDLNFKHLKL 258
antiterm_UpxY NF033644
UpxY family transcription antiterminator; The UpxY family of NusG-related transcription ...
 5-160 1.53e-13

UpxY family transcription antiterminator; The UpxY family of NusG-related transcription antiterminators was described originally from a paralogous family of eight members from Bacteriodes fragilis, UpaY to UphY, each of which was associated with a distinct capsular polysaccharide biosynthesis locus. There is no UpxY protein per se.


Pssm-ID: 468125 [Multi-domain]  Cd Length: 162  Bit Score: 64.80  E-value: 1.53e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIKEqaaqKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSdDAWHLVKDTPK 84
Cdd:NF033644   1 WYALYTRPRREKKVAELLEK----KGI-----ESFLPMQKEIRQWSDRKKRVEVPLIPNLVFVHITEK-ELDEVLEQTPG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  85 VTGFL---GNKNKPSPITEAEAQRI---VNQVKEGVEkprKPAVVFEVGEQVRVADGPFTSFMGTVEEVdeeRGRVKVSV 158
Cdd:NF033644  71 VVRYIrddRGKSKPAIIPDKQMERFrlmLDPSDEVVV---YLEAPLKKGDKVRVIGGPLKGLEGELVRV---KGKKRVVV 144


                 ..
gi 759621471 159 SI 160
Cdd:NF033644 145 RI 146
NGN_SP_TaA cd09893
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), TaA; ...
 4-102 2.75e-12

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), TaA; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antiterminationn factors. TaA is a NusG SP factor that is required for synthesis of a polyketide antibiotic TA in Myxococcus xanthus. Orthologs of the NusG gene exist in all bacteria, but its functions and requirements are different. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggest that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193582 [Multi-domain]  Cd Length: 95  Bit Score: 59.63  E-value: 2.75e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGFEKKIAQQIkeqaAQKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKdTP 83
Cdd:cd09893    1 SWYALYTRSRHEKKVADRL----AKKGI-----ESFLPLYEVLSRWKDRKKKIKVPLFPGYLFVRFQLDPERLRILK-TP 70

                         90
                 ....*....|....*....
gi 759621471  84 KVTGFLGNKNKPSPITEAE 102
Cdd:cd09893   71 GVVRIVGNSGGPIPIPDEE 89
NGN_Bact_2 cd09889
Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 2; The ...
 4-106 4.93e-12

Bacterial N-Utilization Substance G (NusG) N-terminal (NGN) domain, subgroup 2; The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and associates with RNA polymerase elongation and Rho-termination. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. The NusG N-terminal domain (NGN) is quite similar in all NusG orthologs, but its C-terminal domain and the linker that separates these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193578 [Multi-domain]  Cd Length: 100  Bit Score: 59.26  E-value: 4.93e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGFEKKIAQQIKEQaaqkGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:cd09889    1 MWYVVQVRTGREKAVLELLEKL----VGPDVLQECFIPQYERKKRSQGVWRERKYTLFPGYVFVVTDDIDELYYELKRVP 76

                         90       100
                 ....*....|....*....|...
gi 759621471  84 KVTGFLGNKNKPSPITEAEAQRI 106
Cdd:cd09889   77 GFTRLLGNDGSFFPLTPEEADFI 99
NGN_SP cd09886
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The ...
 4-106 1.29e-11

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP); The N-Utilization Substance G (NusG) protein is involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggest that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193575 [Multi-domain]  Cd Length: 97  Bit Score: 58.15  E-value: 1.29e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGfekkiaqqiKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSdDAWHL--VKD 81
Cdd:cd09886    1 SWYALRTNPG---------CEQRAEEALEARGVEAFLPMLTEERKRRRKKFDVERPLFPGYVFARLDRS-KGQDTstIRA 70

                         90       100
                 ....*....|....*....|....*
gi 759621471  82 TPKVTGFLGNKNKPSPITEAEAQRI 106
Cdd:cd09886   71 CDGVLGVVGFDGRPAPVPEQEMRDL 95
NGN_SP_UpxY cd09895
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; ...
 4-106 9.87e-08

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), UpxY; The N-Utilization Substance G (NusG) proteins are involved in transcription elongation and termination. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS (lipopolysaccharide) biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. UpxY proteins, UpxY proteins, where the x is replaced by the letter designation of the specific polysaccharide (UpaY to UphY), are a family of NusG SP factors that act specifically in transcriptional antitermination of operons from which they are encoded. UpxYs are necessary and specific for transcription regulation of the polysaccharide biosynthesis operon. Orthologs of the NusG gene exist in all bacteria, but their functions and requirements are different. The NusG N-terminal (NGN) domain is similar in all NusG orthologs, but its C-terminal domain and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193584 [Multi-domain]  Cd Length: 95  Bit Score: 47.57  E-value: 9.87e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGFEKKIAQQIKEqaaqKGLgdqfdEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAwhLVKDTP 83
Cdd:cd09895    1 PWYALYTFPRREKKVAEYLEK----KGI-----ECFLPMQYEVRQWSGRKKRVEVPLFPNLVFVHITREELD--EVLETP 69

                         90       100
                 ....*....|....*....|....
gi 759621471  84 KVTGFLGNKNK-PSPITEAEAQRI 106
Cdd:cd09895   70 GVVRFVRYRGKePAIIPDKQMESF 93
NGN_SP_RfaH cd09892
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; ...
 4-98 7.71e-07

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), RfaH; RfaH is an operon-specific virulence regulator, thought to have arisen from an early duplication of N-Utilization Substance G (NusG). Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. In contrast, RfaH is a non-essential protein that controls expression of operons containing an ops (operon polarity suppressor) element in their transcribed DNA. RfaH and NusG are different in their response to Rho-dependent terminators and regulatory targets. The NusG N-terminal (NGN) domain is quite similar in all NusG orthologs, but its C-terminal domains and the linker that separate these two domains are different. The domain organization of NusG and its homologs suggest that the common properties of NusG and RfaH are due to their similar NGN domains.


Pssm-ID: 193581 [Multi-domain]  Cd Length: 96  Bit Score: 45.24  E-value: 7.71e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   4 RWYVVHVYSGfekkiaqqiKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:cd09892    1 AWYLLYTKPR---------QEERAAENLERQGFEVFLPMIRVEKRRRGKRTVVTEPLFPGYLFVRLDPEVQNWRPIRSTR 71

                         90
                 ....*....|....*
gi 759621471  84 KVTGFLGNKNKPSPI 98
Cdd:cd09892   72 GVSRLVRFGGEPAPV 86
NGN_plant cd09890
Plant N-Utilization Substance G (NusG) N-terminal (NGN) domain; The N-Utilization Substance G ...
 5-98 9.30e-07

Plant N-Utilization Substance G (NusG) N-terminal (NGN) domain; The N-Utilization Substance G (NusG) protein and its eukaryotic homolog, Spt5, are involved in transcription elongation and termination. NusG contains a NGN domain at its N-terminus and Kyrpides Ouzounis and Woese (KOW) repeats at its C-terminus in bacteria and archaea. The eukaryotic ortholog, Spt5, is a large protein comprising an acidic N-terminus, an NGN domain, and multiple KOW motifs at its C-terminus. Spt5 forms an Spt4-Spt5 complex that is an essential RNA polymerase II elongation factor. The bacterial infected plants contain bacterial DNA, such as NGN sequences, that can be used to clone the DNA of uncultured organisms.


Pssm-ID: 193579  Cd Length: 113  Bit Score: 45.42  E-value: 9.30e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   5 WYVVHVYSGFEKKIAQQIKEQAAQKGLGDQFdEILVPAEEV-TEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDTP 83
Cdd:cd09890    2 WYMLRVPAGRENQAAEALERALATEFPDREF-EVWVPSIPVdRKLKNGSISVKEKPLFPGYVLLRCVLNKEVYDFIRDND 80

                         90       100
                 ....*....|....*....|....
gi 759621471  84 KVTGFLGNK---------NKPSPI 98
Cdd:cd09890   81 SVYGFVGSKvgktgkrqiEIPRPV 104
nusG PRK08559
transcription antitermination protein NusG; Validated
 6-158 3.37e-05

transcription antitermination protein NusG; Validated


Pssm-ID: 181467 [Multi-domain]  Cd Length: 153  Bit Score: 41.78  E-value: 3.37e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   6 YVVHVYSGFEKKIAQQIKEQAAQKGLGdqFDEILVPaEEVTevrrgqkvnserkffpGYVLVKMEMSDDAWHLVKDTPKV 85
Cdd:PRK08559   9 FAVKTTAGQERNVALMLAMRAKKENLP--IYAILAP-PELK----------------GYVLVEAESKGAVEEAIRGIPHV 69

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 759621471  86 TGFLgnknkPSPITEAEAQRIVnqvkegveKPRKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSV 158
Cdd:PRK08559  70 RGVV-----PGEISFEEVEHFL--------KPKPIVEGIKEGDIVELIAGPFKGEKARVVRVDESKEEVTVEL 129
KOW_elon_Spt5 TIGR00405
transcription elongation factor Spt5; This protein contains a KOW domain, shared by bacterial ...
 6-174 6.16e-04

transcription elongation factor Spt5; This protein contains a KOW domain, shared by bacterial NusG and the uL24 (previously L24p/L26e) family of ribosomal proteins. The most recent papers and crystal structures make this a transcription elongation factor rather than a ribosomal protein.


Pssm-ID: 129499 [Multi-domain]  Cd Length: 145  Bit Score: 38.33  E-value: 6.16e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471    6 YVVHVYSGFEKKIAQQIKEQAAQKGLgdQFDEILVPAEevtevrrgqkvnserkfFPGYVLVKMEMSDDAWHLVKDTPKV 85
Cdd:TIGR00405   1 FAVKTSVGQEKNVARLMARKARKSGL--EVYSILAPES-----------------LKGYILVEAETKIDMRNPIIGVPHV 61

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   86 TGFLgnknkPSPITEAEAQRIVnqvkegveKPRKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERGRVKVSVSIFGRST 165
Cdd:TIGR00405  62 RGVV-----EGEIDFEEIERFL--------TPKKIIESIKKGDIVEIISGPFKGERAKVIRVDESKEEVTLELIEAAVPI 128


                  ....*....
gi 759621471  166 PVELEYTQV 174
Cdd:TIGR00405 129 PVTVKGDQV 137
KOW pfam00467
KOW motif; This family has been extended to coincide with ref. The KOW (Kyprides, Ouzounis, ...
 126-156 4.22e-03

KOW motif; This family has been extended to coincide with ref. The KOW (Kyprides, Ouzounis, Woese) motif is found in a variety of ribosomal proteins and NusG.


Pssm-ID: 425698 [Multi-domain]  Cd Length: 32  Bit Score: 33.51  E-value: 4.22e-03
                          10        20        30
                  ....*....|....*....|....*....|.
gi 759621471  126 VGEQVRVADGPFTSFMGTVEEVDEERGRVKV 156
Cdd:pfam00467   1 KGDVVRVIAGPFKGKVGKVVEVDDKKNRVLV 31
NGN_SP_AnfA1 cd09894
N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), AnFA1; ...
 3-112 5.50e-03

N-Utilization Substance G (NusG) N-terminal domain in the NusG Specialized Paralog (SP), AnFA1; Regulation of the afp, antifeeding prophage, gene cluster is mediated by AnFA1, a RfaH-like transcriptional antiterminator. RfaH is an operon-specific virulence regulator, thought to arisen from an early duplication of N-Utilization Substance G (NusG). NusG is essential in Escherichia coli and is associated with RNA polymerase elongation and Rho-termination in bacteria. Paralogs of eubacterial NusG, NusG SP (Specialized Paralog of NusG), are more diverse and often found as the first ORF in operons encoding secreted proteins and LPS biosynthesis genes. NusG SP family members are operon-specific transcriptional antitermination factors. Orthologs of the NusG gene exist in all bacteria, but their functions and requirements are different. The NusG N-terminal domain (NGN) is similar in all NusG orthologs, but its C-terminal domain and the linker that separate these two domains are different. The domain organization of NusG and its orthologs suggests that the common properties of NusG and its orthologs and paralogs are due to their similar NGN domains.


Pssm-ID: 193583 [Multi-domain]  Cd Length: 99  Bit Score: 34.95  E-value: 5.50e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   3 KRWYVVHVYSGFEkkiaQQIKEQAAQKGLgdqfdEILVPaEEVTEVRR---GQKVNSERKFFPGYVLVKMEMsdDAWHLV 79
Cdd:cd09894    1 KRWYLLRCKSGKI----QSVIFSLERLGV-----EVFCP-MIRTRRKRtdcKSYREKIEPLFPGYLFVRFDP--EVVHTS 68

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 759621471  80 KDT--PKVTGFLGNKNKPSPITEAeaqrIVNQVKE 112
Cdd:cd09894   69 KITlaSGVSGFVRFGGEPCPVPDA----VIRALML 99
rfaH PRK09014
transcription/translation regulatory transformer protein RfaH;
3-176 5.61e-03

transcription/translation regulatory transformer protein RfaH;


Pssm-ID: 181611 [Multi-domain]  Cd Length: 162  Bit Score: 35.64  E-value: 5.61e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471   3 KRWYVVHVYSGfekkiaqqiKEQAAQKGLGDQFDEILVPAEEVTEVRRGQKVNSERKFFPGYVLVKMEMSDDAWHLVKDT 82
Cdd:PRK09014   2 KSWYLLYCKRG---------QLQRAQEHLERQGVECLYPMITLEKIVRGKRTEVSEPLFPNYLFVEFDPEVIHTTTIRST 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 759621471  83 PKVTGFLGNKNKPSPIteaeAQRIVNQVKEGVEKPRKPAVVFEVGEQVRVADGPFTSFMGTVEEVDEERgRVKVSVSIFG 162
Cdd:PRK09014  73 RGVSHFVRFGAQPAIV----PSDVIYQLSVYKPEKIVDPETPKPGDKVIITEGAFEGLQAIYTEPDGEA-RSILLLNLLN 147

                        170
                 ....*....|....
gi 759621471 163 RSTPVELEYTQVEK 176
Cdd:PRK09014 148 KQVKHSVDNTQFRK 161
KOW smart00739
KOW (Kyprides, Ouzounis, Woese) motif; Motif in ribosomal proteins, NusG, Spt5p, KIN17 and T54.
 124-150 9.72e-03

KOW (Kyprides, Ouzounis, Woese) motif; Motif in ribosomal proteins, NusG, Spt5p, KIN17 and T54.


Pssm-ID: 128978  Cd Length: 28  Bit Score: 32.30  E-value: 9.72e-03
                           10        20
                   ....*....|....*....|....*..
gi 759621471   124 FEVGEQVRVADGPFTSFMGTVEEVDEE 150
Cdd:smart00739   2 FEVGDTVRVIAGPFKGKVGKVLEVDGE 28
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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