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Conserved domains on  [gi|1079387570|ref|WP_070162529|]
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MULTISPECIES: translation initiation factor IF-1 [Actinomycetes]

Protein Classification

translation initiation factor IF-1( domain architecture ID 10011353)

initiation factor 1 together with initiation factor 2 and 3 play an important role in the correct assembly of the functional initiation complex for protein biosynthesis in prokaryotes

Gene Ontology:  GO:0043022|GO:0019843|GO:0003743

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
infA PRK00276
translation initiation factor IF-1; Validated
 3-73 1.71e-43

translation initiation factor IF-1; Validated


:

Pssm-ID: 178954  Cd Length: 72  Bit Score: 134.51  E-value: 1.71e-43
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1079387570  3 KKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:PRK00276   2 AKEDVIEMEGTVVEALPNAMFRVELENGHEVLAHISGKMRKNYIRILPGDKVTVELSPYDLTKGRITYRHK 72
 
Name Accession Description Interval E-value
infA PRK00276
translation initiation factor IF-1; Validated
 3-73 1.71e-43

translation initiation factor IF-1; Validated


Pssm-ID: 178954  Cd Length: 72  Bit Score: 134.51  E-value: 1.71e-43
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1079387570  3 KKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:PRK00276   2 AKEDVIEMEGTVVEALPNAMFRVELENGHEVLAHISGKMRKNYIRILPGDKVTVELSPYDLTKGRITYRHK 72
InfA COG0361
Translation initiation factor IF-1 [Translation, ribosomal structure and biogenesis]; ...
 1-73 7.49e-40

Translation initiation factor IF-1 [Translation, ribosomal structure and biogenesis]; Translation initiation factor IF-1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440130  Cd Length: 73  Bit Score: 125.22  E-value: 7.49e-40
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1079387570  1 MGKKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:COG0361    1 MAKKEDLIEVEGVVEEVLPNARFRVRLENGHERLARISGKMRKNRIRILPGDRVLVEPSPYDLTKGRIVYRYK 73
infA TIGR00008
translation initiation factor IF-1; This family consists of translation initiation factor IF-1 ...
 4-72 7.19e-38

translation initiation factor IF-1; This family consists of translation initiation factor IF-1 as found in bacteria and chloroplasts. This protein, about 70 residues in length, consists largely of an S1 RNA binding domain (pfam00575). [Protein synthesis, Translation factors]


Pssm-ID: 188015  Cd Length: 69  Bit Score: 120.13  E-value: 7.19e-38
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1079387570  4 KEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:TIGR00008  1 KEDKIEMEGKVTESLPNAMFRVELENGHEVLAHISGKIRMHYIRILPGDRVKVELSPYDLTRGRITYRL 69
S1_IF1 cd04451
S1_IF1: Translation Initiation Factor IF1, S1-like RNA-binding domain. IF1 contains an S1-like ...
 8-71 9.40e-34

S1_IF1: Translation Initiation Factor IF1, S1-like RNA-binding domain. IF1 contains an S1-like RNA-binding domain, which is found in a wide variety of RNA-associated proteins. Translation initiation includes a number of interrelated steps preceding the formation of the first peptide bond. In Escherichia coli, the initiation mechanism requires, in addition to mRNA, fMet-tRNA, and ribosomal subunits, the presence of three additional proteins (initiation factors IF1, IF2, and IF3) and at least one GTP molecule. The three initiation factors influence both the kinetics and the stability of ternary complex formation. IF1 is the smallest of the three factors. IF1 enhances the rate of 70S ribosome subunit association and dissociation and the interaction of 30S ribosomal subunit with IF2 and IF3. It stimulates 30S complex formation. In addition, by binding to the A-site of the 30S ribosomal subunit, IF1 may contribute to the fidelity of the selection of the initiation site of the mRNA.


Pssm-ID: 239898  Cd Length: 64  Bit Score: 109.53  E-value: 9.40e-34
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079387570  8 IEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYR 71
Cdd:cd04451    1 IEMEGVVTEALPNAMFRVELENGHEVLAHISGKMRMNYIRILPGDRVKVELSPYDLTKGRIVYR 64
eIF-1a pfam01176
Translation initiation factor 1A / IF-1; This family includes both the eukaryotic translation ...
 9-71 2.22e-27

Translation initiation factor 1A / IF-1; This family includes both the eukaryotic translation factor eIF-1A and the bacterial translation initiation factor IF-1.


Pssm-ID: 460099  Cd Length: 62  Bit Score: 93.60  E-value: 2.22e-27
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1079387570  9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRqHYIRILPEDRVVVEMSPYDLNRGRIVYR 71
Cdd:pfam01176  1 EVIGKVVEALGNNLFEVELDNGHTRLAHIPGKMR-NRIWIKRGDYVLVEPSPYDLTKGRIVYR 62
eIF1a smart00652
eukaryotic translation initiation factor 1A;
9-72 2.58e-04

eukaryotic translation initiation factor 1A;


Pssm-ID: 128900  Cd Length: 83  Bit Score: 35.69  E-value: 2.58e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079387570   9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHyIRILPEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:smart00652  6 QEIAQVVKMLGNGRLEVMCADGKERLARIPGKMRKK-VWIRRGDIVLVDPWDFQDVKADIIYKY 68
 
Name Accession Description Interval E-value
infA PRK00276
translation initiation factor IF-1; Validated
 3-73 1.71e-43

translation initiation factor IF-1; Validated


Pssm-ID: 178954  Cd Length: 72  Bit Score: 134.51  E-value: 1.71e-43
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1079387570  3 KKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:PRK00276   2 AKEDVIEMEGTVVEALPNAMFRVELENGHEVLAHISGKMRKNYIRILPGDKVTVELSPYDLTKGRITYRHK 72
InfA COG0361
Translation initiation factor IF-1 [Translation, ribosomal structure and biogenesis]; ...
 1-73 7.49e-40

Translation initiation factor IF-1 [Translation, ribosomal structure and biogenesis]; Translation initiation factor IF-1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440130  Cd Length: 73  Bit Score: 125.22  E-value: 7.49e-40
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1079387570  1 MGKKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:COG0361    1 MAKKEDLIEVEGVVEEVLPNARFRVRLENGHERLARISGKMRKNRIRILPGDRVLVEPSPYDLTKGRIVYRYK 73
infA TIGR00008
translation initiation factor IF-1; This family consists of translation initiation factor IF-1 ...
 4-72 7.19e-38

translation initiation factor IF-1; This family consists of translation initiation factor IF-1 as found in bacteria and chloroplasts. This protein, about 70 residues in length, consists largely of an S1 RNA binding domain (pfam00575). [Protein synthesis, Translation factors]


Pssm-ID: 188015  Cd Length: 69  Bit Score: 120.13  E-value: 7.19e-38
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1079387570  4 KEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:TIGR00008  1 KEDKIEMEGKVTESLPNAMFRVELENGHEVLAHISGKIRMHYIRILPGDRVKVELSPYDLTRGRITYRL 69
infA CHL00010
translation initiation factor 1
 3-73 2.73e-35

translation initiation factor 1


Pssm-ID: 214332  Cd Length: 78  Bit Score: 113.93  E-value: 2.73e-35
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1079387570  3 KKEGVIEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:CHL00010   2 KKENKIEMEGLVTESLPNGMFRVRLDNGCQVLGYISGKIRRNSIRILPGDRVKVELSPYDLTKGRIIYRLR 72
S1_IF1 cd04451
S1_IF1: Translation Initiation Factor IF1, S1-like RNA-binding domain. IF1 contains an S1-like ...
 8-71 9.40e-34

S1_IF1: Translation Initiation Factor IF1, S1-like RNA-binding domain. IF1 contains an S1-like RNA-binding domain, which is found in a wide variety of RNA-associated proteins. Translation initiation includes a number of interrelated steps preceding the formation of the first peptide bond. In Escherichia coli, the initiation mechanism requires, in addition to mRNA, fMet-tRNA, and ribosomal subunits, the presence of three additional proteins (initiation factors IF1, IF2, and IF3) and at least one GTP molecule. The three initiation factors influence both the kinetics and the stability of ternary complex formation. IF1 is the smallest of the three factors. IF1 enhances the rate of 70S ribosome subunit association and dissociation and the interaction of 30S ribosomal subunit with IF2 and IF3. It stimulates 30S complex formation. In addition, by binding to the A-site of the 30S ribosomal subunit, IF1 may contribute to the fidelity of the selection of the initiation site of the mRNA.


Pssm-ID: 239898  Cd Length: 64  Bit Score: 109.53  E-value: 9.40e-34
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079387570  8 IEVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHYIRILPEDRVVVEMSPYDLNRGRIVYR 71
Cdd:cd04451    1 IEMEGVVTEALPNAMFRVELENGHEVLAHISGKMRMNYIRILPGDRVKVELSPYDLTKGRIVYR 64
eIF-1a pfam01176
Translation initiation factor 1A / IF-1; This family includes both the eukaryotic translation ...
 9-71 2.22e-27

Translation initiation factor 1A / IF-1; This family includes both the eukaryotic translation factor eIF-1A and the bacterial translation initiation factor IF-1.


Pssm-ID: 460099  Cd Length: 62  Bit Score: 93.60  E-value: 2.22e-27
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1079387570  9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRqHYIRILPEDRVVVEMSPYDLNRGRIVYR 71
Cdd:pfam01176  1 EVIGKVVEALGNNLFEVELDNGHTRLAHIPGKMR-NRIWIKRGDYVLVEPSPYDLTKGRIVYR 62
S1_IF1A cd05793
S1_IF1A: Translation initiation factor IF1A, also referred to as eIF1A in eukaryotes and aIF1A ...
 9-72 2.26e-05

S1_IF1A: Translation initiation factor IF1A, also referred to as eIF1A in eukaryotes and aIF1A in archaea, S1-like RNA-binding domain. S1-like RNA-binding domains are found in a wide variety of RNA-associated proteins. IF1A is essential for translation initiation. eIF1A acts synergistically with eIF1 to mediate assembly of ribosomal initiation complexes at the initiation codon and maintain the accuracy of this process by recognizing and destabilizing aberrant preinitiation complexes from the mRNA. Without eIF1A and eIF1, 43S ribosomal preinitiation complexes can bind to the cap-proximal region, but are unable to reach the initiation codon. eIF1a also enhances the formation of 5'-terminal complexes in the presence of other translation initiation factors. This protein family is only found in eukaryotes and archaea.


Pssm-ID: 240219  Cd Length: 77  Bit Score: 38.27  E-value: 2.26e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079387570  9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHyIRILPEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:cd05793    1 EEYGQVEKMLGNGRLEVRCFDGKKRLCRIRGKMRKR-VWINEGDIVLVAPWDFQDDKADIIYKY 63
eIF1a smart00652
eukaryotic translation initiation factor 1A;
9-72 2.58e-04

eukaryotic translation initiation factor 1A;


Pssm-ID: 128900  Cd Length: 83  Bit Score: 35.69  E-value: 2.58e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1079387570   9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQHyIRILPEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:smart00652  6 QEIAQVVKMLGNGRLEVMCADGKERLARIPGKMRKK-VWIRRGDIVLVDPWDFQDVKADIIYKY 68
S1 smart00316
Ribosomal protein S1-like RNA-binding domain;
10-73 7.49e-04

Ribosomal protein S1-like RNA-binding domain;


Pssm-ID: 197648 [Multi-domain]  Cd Length: 72  Bit Score: 34.12  E-value: 7.49e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1079387570  10 VEGRVTEALPNaMFRVRLENDHVVLATISGKMRQH----YIRILPEDRVVVEMSPYDLNRGRIVYRYK 73
Cdd:smart00316  6 VEGTVTEITPG-GAFVDLGNGVEGLIPISELSDKRvkdpEEVLKVGDEVKVKVLSVDEEKGRIILSLK 72
PRK04012 PRK04012
translation initiation factor IF-1A; Provisional
 9-72 4.18e-03

translation initiation factor IF-1A; Provisional


Pssm-ID: 179708  Cd Length: 100  Bit Score: 32.62  E-value: 4.18e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1079387570   9 EVEGRVTEALPNAMFRVRLENDHVVLATISGKMRQH-YIRIlpEDRVVVEMSPYDLNRGRIVYRY 72
Cdd:PRK04012   22 EVFGVVEQMLGANRVRVRCMDGVERMGRIPGKMKKRmWIRE--GDVVIVAPWDFQDEKADIIWRY 84
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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