Multidrug resistance protein;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAG-AYIADIT 124
Cdd:TIGR00880   1 GLLLAGYALGQLIYSPLSGLLTDRFGRKPVLLVGLFIFVLSTAMFALSSNITVLIIARFLQGFGAAFALVAGaALIADIY 80

                  .
gi 1432233657 125 D 125
Cdd:TIGR00880  81 P 81

Major Facilitator Superfamily;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  10 ILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAhyGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI 89
Cdd:pfam07690   1 LFLAAFLAALGRSLLGPALPLLLAEDLGISPTEI--GLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFALGLLL 78

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1432233657  90 MATAPFLWVLYIGRIVAGITGATGAVAGAYIA 121
Cdd:pfam07690  79 LLFASSLWLLLVLRVLQGLGAGALFPAALALI 110

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  13 TVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMAT 92
Cdd:cd17330     4 VVFLDALGFGIVFPVLPFYVREFNKLGASGYYVGLLQSSYSLGQFLASPLWGRLSDRYGRKPVLLLSLAGSAVSYVLLGL 83

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1432233657  93 APFLWVLYIGRIVAGITGATGAVAGAYIADITD 125
Cdd:cd17330    84 SRNLWLLFLARFLDGLTGGNVSVAQAYIADITD 116

Major facilitator superfamily domain-containing protein 10; Major facilitator superfamily domain-containing protein 10 (MFSD10) is also called tetracycline transporter-like protein (TETRAN). It is expressed in various human tissues, including the kidney. In cultured cells, its overexpression facilitated the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs). MFSD10/TETRAN overexpression cause resistance to some NSAIDs, suggesting that it may be an organic anion transporter that serves as an efflux pump for some NSAIDs and various other organic anions at the final excretion step. MFSD10 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   7 LIVILSTVALDAVGIGLIMPVLPGLL---------------RDLVHS------NDVTAHY------GILLALYALMQFAC 59
Cdd:cd17389     1 ITIVFLALLLDLLAFTIILPLFPSLLdyygqnddsglyslqISSVDWfrdaigAPDERRYnsvlfgGLIGSLFSLLQFLS 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1432233657  60 APVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITD 125
Cdd:cd17389    81 SPITGAASDVYGRKPVLLLTMIGLAISYALWAVSRSFTIFLLSRIVGGISKGNVSLSTAIVADVTS 146

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  10 ILSTVALDAVGIGLIMPVLPGLLRDLVHSNdvtAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI 89
Cdd:cd17325     1 LSLALFLDMLGYGIIIPILPLYAESLGASV---AQIGLIVAAYALAQLLFSPPAGRLSDRFGRKRLLLLGLLLLAVSTLL 77

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1432233657  90 MATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITD 125
Cdd:cd17325    78 FAFATSYWQLLLARFLQGLaSAAVWPAAMALIADIAP 114

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNdvTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGA 83
Cdd:cd17325   198 DRRLLALFLAIFVLAFAFGALEPFLPLYAAELGGLS--PAQIGLLFGAQGLASALSQPPAGKLSDRIGRKPLILIGLLLS 275

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 1432233657  84 AVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADITD 125
Cdd:cd17325   276 AVALLLLPLATSFWLLLLLLALLGLgLGLVFPATLALLADIVP 318

Solute carrier family 22 member 18 of the Major Facilitator Superfamily of transporters; Solute carrier family 22 member 18 (SLC22A18) is also called Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein (BWR1A or BWSCR1A), efflux transporter-like protein, imprinted multi-membrane-spanning polyspecific transporter-related protein 1 (IMPT1), organic cation transporter-like protein 2 (ORCTL2), or tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein (TSSC5). It is localized at the apical membrane surface of renal proximal tubules and may act as an organic cation/proton antiporter. It functions as a tumor suppressor in several cancer types including glioblastoma and colorectal cancer. SLC22A18 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   6 PLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNdvtAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAV 85
Cdd:cd17331     1 VLLIVHLNIVLYATCYWLQQPVLPYLTKKLGVDS---VVFGYLQTTFSVLQLVGGPLFGRFGDLFGPRAALVLSFLSSAL 77

                          90       100
                  ....*....|....*....|.
gi 1432233657  86 DYAIMATAPFLWVLYIGRIVA 106
Cdd:cd17331    78 SYLLLAFASSVPMLFLSRLPS 98

Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; This family includes a group of putative arabinose efflux permease family transporters, such as alpha proteobacterium quinolone resistance protein NorA (characterized Staphylococcus aureus Quinolone resistance protein NorA belongs to a different group), Desulfovibrio dechloracetivorans bacillibactin exporter, Vibrio aerogenes antiseptic resistance protein. The biological function of those transporters remain unclear. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAhyGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGaavd 86
Cdd:cd17473     1 LITLLLASTLTVMAGAAIAPALPAIAQHFVPNSELLV--PLLLTLPALFIALFAPLAGWLADRFGRKRLLIAGLLL---- 74

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|.
gi 1432233657  87 YAIMATAPF----LWVLYIGRIVAGI-TGATGAVAGAYIAD 122
Cdd:cd17473    75 YGIGGVAGLfldsLYALLASRALLGIgVAGIMTLATALIGD 115

Multidrug transporter MdfA and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as MdfA (also called chloramphenicol resistance pump Cmr), EmrD, MdtM, MdtL, bicyclomycin resistance protein (also called sulfonamide resistance protein), and the uncharacterized inner membrane transport protein YdhC. EmrD is a proton-dependent secondary transporter, first identified as an efflux pump for uncouplers of oxidative phosphorylation. It expels a range of drug molecules and amphipathic compounds across the inner membrane of E. coli. Similarly, MdfA is a secondary multidrug transporter that exports a broad spectrum of structurally and electrically dissimilar toxic compounds. These MDR transporters are drug/H+ antiporters (DHA) belonging to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   7 LIVILSTVALDAVGIGLIMPVLPGLLRDL-VHSNDVTAHYGILLALYALMQFacapVLGALSDRFGRRPVLLVSLAGAAV 85
Cdd:cd17320     1 LLLLALLVALGPLAIDMYLPALPAIARDLgASASAVQLTLSAYLLGFALGQL----LYGPLSDRFGRRPVLLAGLLLFIL 76

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1432233657  86 DYAIMATAPFLWVLYIGRIVAGITGATGAV-AGAYIADI 123
Cdd:cd17320    77 ASLLCALASSIEQLLIARFLQGLGAAAGSVvARAIVRDL 115

Major facilitator superfamily domain-containing 14A and 14B; This subfamily is composed of major facilitator superfamily domain-containing 14A (MFSD14A) and MFSD14B, and similar proteins. MFSD14A and MFSD14B are also called hippocampus abundant transcript 1 protein (HIAT1) and hippocampus abundant transcript-like protein 1 (HIATL1), respectively. They are both ubiquitously expressed with HIAT1 highly expressed intestis and HIATL1 most abundantly expressed in skeletal muscle. Gene disruption of MFSD14A causes globozoospermia and infertility in male mice. It has bee suggested that MFSD14A may transport a solute from the bloodstream that is required for spermiogenesis. The function of MFSD14B is unknown. The MFSD14 subfamily belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  46 GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPflWVLYIGRIVAGITGATGAVAGAYIADITD 125
Cdd:cd17387    39 GLVQGVKGILSFLSAPLIGALSDVWGRKSFLLLTVFFTCAPIPLMRISP--WWYFAMISVSGIFSVTFSVIFAYVADITT 116

H(+)-myo-inositol cotransporter and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of myo-inositol/inositol transporters and similar transporters from vertebrates, plant, and fungi. The human protein is called H(+)-myo-inositol cotransporter/Proton myo-inositol cotransporter (HMIT), or H(+)-myo-inositol symporter, or Solute carrier family 2 member 13 (SLC2A13). HMIT is classified as a Class 3 GLUT (glucose transporter) based on sequence similarity with GLUTs, but it does not transport glucose. It specifically transports myo-inositol and is expressed predominantly in the brain, with high expression in the hippocampus, hypothalamus, cerebellum and brainstem. HMIT may be involved in regulating processes that require high levels of myo-inositol or its phosphorylated derivatives, such as membrane recycling, growth cone dynamics, and synaptic vesicle exocytosis. Arabidopsis Inositol transporter 4 (AtINT4) mediates high-affinity H+ symport of myo-inositol across the plasma membrane. The HMIT-like subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  22 GLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYI 101
Cdd:cd17360    14 GVISGALLYIKDDLGEVVLSTGWQELIVSSTVAGAAVGAAIGGWLNDRFGRRPCILLADALFTIGAIVMAAAPNKEVLIV 93

                          90       100
                  ....*....|....*....|....
gi 1432233657 102 GRIVAGI-TGATGAVAGAYIADIT 124
Cdd:cd17360    94 GRVLVGLgVGIASMTVPLYIAEAA 117

Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAhyGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVD 86
Cdd:cd17329     1 LWVLLIGSFLNRLGFFMVWPFMAIYLHQQLGLSASIV--GLVLALSAVAGIVASLIGGRLADRFGRKPVMLAGLLLRALG 78

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1432233657  87 YAIMATAPFLWVLYIGRIVAGITGATGAVAG-AYIADIT 124
Cdd:cd17329    79 FALLGFAHSPWLFAIALVLTGFGGGLFEPASeAMIADVT 117

cation transport protein; [Transport and binding proteins, Cations and iron carrying compounds]

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1432233657  60 APVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAG 117
Cdd:TIGR00898 144 SFVFGYLSDRFGRKKVLLLSTLVTAVSGVLTAFSPNYTVFLVFRLLVGMGIGGIWVQA 201

Bacillus subtilis multidrug efflux protein YfmO and similar transporters of the Major Facilitator Superfamily; This family is composed of Bacillus subtilis multidrug efflux protein YfmO, bacillibactin exporter YmfD/YmfE, uncharacterized MFS-type transporter YvmA, and similar proteins. YfmO acts to efflux copper or a copper complex, and could contribute to copper resistance. YmfD/YmfE is involved in secretion of bacillibactin. The YfmO-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   8 IVILSTVALDAV-GIGLIMPVLPGLLRDLVHSndvTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVD 86
Cdd:cd17474     1 LYAVFFATLLAVlGVSIVDPILPAIAEALGAS---PAQVGLLITAFTLPAAVLMPVAGVLADRFGRKKVLLPGLLLFGIG 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1432233657  87 YAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADI 123
Cdd:cd17474    78 GLLCGLSDSFALLLAGRALQGIgAAGLGPLALTLIGDL 115

Sugar (and other) transporter;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  45 YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPF---LWVLYIGRIVAGI-TGATGAVAGAYI 120
Cdd:pfam00083  48 SGLIVSIFSVGCFIGSLFAGKLGDRFGRKKSLLIANVLFVIGAVLQGAAKGkwsVYQLIVGRVLVGIgVGGASVLAPMYI 127

                  ....
gi 1432233657 121 ADIT 124
Cdd:pfam00083 128 SEIA 131

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNdvTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGA 83
Cdd:COG2814   206 RPRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLS--ASAAGLLLALFGLGGVLGALLAGRLADRFGRRRLLLIGLLLL 283

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1432233657  84 AVDYAIMATAPFLWVLYIGRIVAGIT-GATGAVAGAYIADIT 124
Cdd:COG2814   284 ALGLLLLALAGSLWLLLLALFLLGFGfGLLFPLLQALVAELA 325

Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator Superfamily; This family is composed of glucose transporters (GLUTs) and other sugar transporters including fungal hexose transporters (HXT), bacterial xylose transporter (XylE), plant sugar transport proteins (STP) and polyol transporters (PLT), H(+)-myo-inositol cotransporter (HMIT), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. The GLUT-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1432233657  64 GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI-TGATGAVAGAYIADI 123
Cdd:cd17315    55 GPLADRFGRRKSLLIAAVLYVIGALLSALAPNVWVLIVGRFLLGLgVGLASVLVPLYISEI 115

MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and metabolism, Amino acid transport and metabolism, Inorganic ion transport and metabolism, General function prediction only];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSndvTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAA 84
Cdd:COG0477    14 RALLALALGTFLEGLDFTIVNVALPSIAADLGAS---SAQLGWIVSAYLLGRAIGLLLFGRLGDRYGRKRVLLIGLLLFG 90

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1432233657  85 VDYAIMATAPFLWVLYIGRIVAGITGATG-AVAGAYIADI 123
Cdd:COG0477    91 LASLLCGLAPSPELLIAARALQGIGAGGLmPGALALIAEL 130

Solute carrier family 46 member 1, also called Proton-coupled folate transporter, of the Major Facilitator Superfamily of transporters; Solute carrier family 46 member 1 (SLC46A1) is also called proton-coupled folate transporter (PCFT), G21, or heme carrier protein 1 (HCP1). It functions in two ways: as an intestinal proton-coupled high-affinity folate transporter that facilitates the absorption of folates across the brush-border membrane of the small intestine; and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. It displays a higher affinity for folate than heme. It is also expressed in the choroid plexus and is required for transport of folates into the cerebrospinal fluid. Loss of function mutations in the SLC46A1 gene results in the autosomal recessive disorder "hereditary folate malabsorption" (HFM), characterized by severe systemic and cerebral folate deficiency. SLC46A1 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  41 VTAHY-------GILLALYALMqfacapVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWV----LYIGRIVAGIT 109
Cdd:cd17449    62 LTAHWnlyinlgGFLVGLFSVT------LLGPWSDSVGRRPVLILPSLGLALQAAVYLLVMYLQLhvayFLIGRILSGLL 135

                          90
                  ....*....|....*....
gi 1432233657 110 GATGAV-AG--AYIADITD 125
Cdd:cd17449   136 GDFNGIlAGcfAYVADVSD 154

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657   7 LIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDvTAHYGILLALYALMQFACAPVLGALSDRFG-RRPVLLVSLAGAAV 85
Cdd:cd17326     1 IYVMYLTMFLSSLQFSIVLTSIWPYLQKIDPTAT-ASFFGWVVAAYSLGQLVFSPVFGYWSNKTSsVKEPLIVSILIAIV 79

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 1432233657  86 D-----YAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADIT 124
Cdd:cd17326    80 GnilyaLAEYFPSNRKYVMLVARFLIGVGAGNVAVLRAYVAAAS 123

YcaD and similar transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MFS-type transporter YcaD, Bacillus subtilis MFS-type transporter YfkF, and similar proteins. They are uncharacterized transporters belonging to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  10 ILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTahyGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAI 89
Cdd:cd17477     1 LLLSVALLGLGSGLLLPLLSLRLEREGVSTALI---GLVASAYYLGILLGSPLVPRLIRRVGHIRAFAAGAAITAVSVLL 77

                          90       100
                  ....*....|....*....|..
gi 1432233657  90 MATAPFLWVLYIGRIVAGITGA 111
Cdd:cd17477    78 LALTDSPWVWFLLRFVMGIGLA 99

putative sialic acid transporter; Provisional

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1432233657  62 VLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGItgatgAVAGAYIADIT 124
Cdd:PRK03893   74 LLGAMGDRYGRRLAMVISIVLFSVGTLACGFAPGYWTLFIARLVIGM-----GMAGEYGSSAT 131

Glucose transporter type 6, a Class 3 GLUT, of the Major Facilitator Superfamily of transporters; Glucose transporter type 6 (GLUT6) is also called Solute carrier family 2, facilitated glucose transporter member 6 (SLC2A6). It is classified as a Class 3 GLUT protein, and is a facilitative glucose transporter that binds cytochalasin B with low affinity. It is found in the brain, spleen, and leucocytes. GLUT6 may function in oxalate secretion. SLC2A6 has been identified as an oxalate nephrolithiasis gene in mice; its deletion causes spontaneous calcium oxalate nephrolithiasis in the setting of hyperoxalaemia, hyperoxaluria, and nephrocalcinosis. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  26 PVLPGLLRDLVHSNDVTAH----YGILLALYAlmqfaCAPVLGA--LSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVL 99
Cdd:cd17434    23 PVIPALEQGDDPRLHLNVHqiswFGSVFTLGA-----AAGGLSAmfLNDRLGRKLSIMFSAVPSALGYLLMGSAQGIWML 97

                          90       100
                  ....*....|....*....|....*.
gi 1432233657 100 YIGRIVAGITGA-TGAVAGAYIADIT 124
Cdd:cd17434    98 LLGRFLTGFAGGiTAASIPVYVSEIS 123

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1432233657  43 AHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIA 121
Cdd:cd17489   227 SNAGLFFTVYAIALLLSRPFSGKLSDRKGPKTVIIPGLLLLALGLLLLSFAGSPWMLLLAAVLYGLGFGLLFPALQALA 305

Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This subfamily is composed of putative multidrug resistance (MDR) transporters including Chlamydia trachomatis antiseptic resistance protein QacA_2, and Serratia sp. DD3 Bmr3. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  23 LIMPVLPGLLRDLvhsNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG 102
Cdd:cd17504    14 MLSPALPTIQREF---GTSAAAVSWVLTAYLLVGAVSTPILGRLGDLYGRKKVLLIVLAIYTAGSLLAAFAPNLPVLIAG 90

                          90
                  ....*....|
gi 1432233657 103 RIVAGITGAT 112
Cdd:cd17504    91 RALQGIGGAV 100

Fungal Hexose transporter subfamily of the Major Facilitator Superfamily of transporters and similar proteins; The fungal hexose transporter (HXT) subfamily is comprised of functionally redundant proteins that function mainly in the transport of glucose, as well as other sugars such as galactose and fructose. Saccharomyces cerevisiae has 20 genes that encode proteins in this family (HXT1 to HXT17, GAL2, SNF3, and RGT2). Seven of these (HXT1-7) encode functional glucose transporters. Gal2p is a galactose transporter, while Rgt2p and Snf3p act as cell surface glucose receptors that initiate signal transduction in response to glucose, functioning in an induction pathway responsible for glucose uptake. Rgt2p is activated by high levels of glucose and stimulates expression of low affinity glucose transporters such as Hxt1p and Hxt3p, while Snf3p generates a glucose signal in response to low levels of glucose, stimulating the expression of high affinity glucose transporters such as Hxt2p and Hxt4p. Schizosaccharomyces pombe contains eight GHT genes (GHT1-8) belonging to this family. Ght1, Ght2, and Ght5 are high-affinity glucose transporters; Ght3 is a high-affinity gluconate transporter; and Ght6 high-affinity fructose transporter. The substrate specificities for Ght4, Ght7, and Ght8 remain undetermined. The HXT subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  42 TAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATA-PFLWVLYIGRIVAGI-TGATGAVAGAY 119
Cdd:cd17356    44 SSRQGLIVSIVNLGSFFGALISSFLSDRIGRKKSIQIGCVIYIIGAIIQVAAiGKWYQLIVGRIIAGLgVGFASVLVPVY 123

                  ....
gi 1432233657 120 IADI 123
Cdd:cd17356   124 QSEV 127

Bacillus subtilis YxlH and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Bacillus subtilis YxlH uncharacterized MFS-type transporter YxlH and similar proteins. The biological function of YxlH remains unclear. The YxlH-like subfamily belongs to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1432233657  25 MPVLPGLLRDLVHSNDVTahyGILLALYALMQFACAPVLGALSDRFGRR-PVLLVSLAGAAVDYAIMATAPFLWVLYIGR 103
Cdd:cd17490    16 VPFLSPYAESLGASLSMI---GIILGAYGISQMLLRIPLGILSDRIGKRkLFIIIGLLFTVLASLIMILFPSPWGLLIAR 92

                  ....*....
gi 1432233657 104 IVAGITGAT 112
Cdd:cd17490    93 FLSGIAAAT 101