Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   5 IIIPAYNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLAMRG 55
Cdd:cd00761     1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAKKD 51

putative glycosyl transferase; Provisional

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   2 KLSIIIPAYNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:PRK10073    7 KLSIIIPLYNAGKDFRAFMESLIAQTWTALEIIIVNDGSTDNSVEIAKHYA 57

glycosyltransferase domain; This model describes a putative glycotransferase domain, related to the group 2 family glycosyltransferases of pfam00535.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1562284756   2 KLSIIIPAYNAAKYVERCVESILSQDFnDLEILIVDdgSSDN 43
Cdd:TIGR04440   1 KLTIIIPTYNRPEYLKRWLRYYSDFGC-DYRIIIAD--SSDE 39

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   5 IIIPAYNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLAMRG 55
Cdd:cd00761     1 VIIPAYNEEPYLERCLESLLAQTYPNFEVIVVDDGSTDGTLEILEEYAKKD 51

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1562284756   1 MKLSIIIPAYNAAKYVERCVESILSQDF--NDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:COG1215    29 PRVSVIIPAYNEEAVIEETLRSLLAQDYpkEKLEVIVVDDGSTDETAEIARELA 82

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1562284756   1 MKLSIIIPAYNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:COG1216     3 PKVSVVIPTYNRPELLRRCLESLLAQTYPPFEVIVVDNGSTDGTAELLAALA 54

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1562284756   4 SIIIPAYNAAKYVERCVESILSQDF--NDLEILIVDDGSSDNTLDVCTQLAMR 54
Cdd:cd02525     3 SIIIPVRNEEKYIEELLESLLNQSYpkDLIEIIVVDGGSTDGTREIVQEYAAK 55

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1562284756   5 IIIPAYNAAKYVERCVESILS--QDFNDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:cd04179     1 VVIPAYNEEENIPELVERLLAvlEEGYDYEIIVVDDGSTDGTAEIARELA 50

CESA_like_1 is a member of the cellulose synthase (CESA) superfamily; This is a subfamily of cellulose synthase (CESA) superfamily. CESA superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members of the superfamily include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*...
gi 1562284756   2 KLSIIIPAYNAAKYVERCVESILSQDF--NDLEILIVDDGSSDNTLDV 47
Cdd:cd06439    30 TVTIIIPAYNEEAVIEAKLENLLALDYprDRLEIIVVSDGSTDGTAEI 77

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   5 IIIPAYNAAKYVERCVESI---LSQDFNDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:cd04187     1 IVVPVYNEEENLPELYERLkavLESLGYDYEIIFVDDGSTDRTLEILRELA 51

GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; Glycosyltransferase family 2 (GT-2) subfamily of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 1562284756   3 LSIIIPAYNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLA 52
Cdd:cd02522     1 LSIIIPTLNEAENLPRLLASLRRLNPLPLEIIVVDGGSTDGTVAIARSAG 50

DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1562284756   5 IIIPAYNAAK----YVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQLAMR 54
Cdd:cd04188     1 VVIPAYNEEKrlppTLEEAVEYLEERPSFSYEIIVVDDGSKDGTAEVARKLARK 54

DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to eukaryotic DPM1, including enzymes from bacteria and archaea; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. This protein family belongs to Glycosyltransferase 2 superfamily.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1562284756   5 IIIPAYNAAKYVERCVESILSQ-DFNDLEILIVDDGSSDNTLDVCTQLAMRGG 56
Cdd:cd06442     1 IIIPTYNERENIPELIERLDAAlKGIDYEIIVVDDNSPDGTAEIVRELAKEYP 53

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 1562284756   5 IIIPAYNAAKYVERCVESILSQDFND--LEILIVDDGSSDNTLDV 47
Cdd:cd04192     1 VVIAARNEAENLPRLLQSLSALDYPKekFEVILVDDHSTDGTVQI 45

dolichyl-phosphate beta-glucosyltransferase; Provisional

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   3 LSIIIPAYNAA-----------KYVERCVESILSQDFndlEILIVDDGSSDNTLDVCTQLA 52
Cdd:PTZ00260   72 LSIVIPAYNEEdrlpkmlketiKYLESRSRKDPKFKY---EIIIVNDGSKDKTLKVAKDFW 129

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1562284756  10 YNAAKYVERCVESILSQDFNDLEILIVDDGSSDNTLDVCTQL 51
Cdd:cd04185     6 YNRLDLLKECLDALLAQTRPPDHIIVIDNASTDGTAEWLTSL 47

hormogonium polysaccharide biosynthesis glycosyltransferase HpsE;

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1562284756   1 MKLSIIIPAYNAAKYVERCVESILSQ----DFNdLEILIVDDGSSDNTLDV 47
Cdd:NF038302    1 LDFTVAIPTYNGANRLPEVLERLRSQigteSLS-WEIIVVDNNSTDNTAQV 50

undecaprenyl phosphate 4-deoxy-4-formamido-L-arabinose transferase; Provisional

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1562284756   2 KLSIIIPAYNAAK----YVERCVESI--LSQDFndlEILIVDDGSSDNTLDVCTQLA 52
Cdd:PRK10714    7 KVSVVIPVYNEQEslpeLIRRTTAACesLGKEY---EILLIDDGSSDNSAEMLVEAA 60

N-acetyl-glucosamine transferase is involved in the synthesis of Poly-beta-1,6-N-acetyl-D-glucosamine; N-acetyl-glucosamine transferase is responsible for the synthesis of bacteria Poly-beta-1,6-N-acetyl-D-glucosamine (PGA). Poly-beta-1,6-N-acetyl-D-glucosamine is a homopolymer that serves as an adhesion for the maintenance of biofilm structural stability in diverse eubacteria. N-acetyl-glucosamine transferase is the product of gene pgaC. Genetic analysis indicated that all four genes of the pgaABCD locus were required for the PGA production, pgaC being a glycosyltransferase.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1562284756   5 IIIPAYNAAKYVERCVESILsQDFNDLEILIVDDGSSDNTLD 46
Cdd:cd06436     1 VLVPCLNEEAVIQRTLASLL-RNKPNFLVLVIDDASDDDTAG 41

Glycosyltransferase like family 2; Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 1562284756   4 SIIIPAYNAAK--YVERCVESILSQDFNDLEILIVDDGSSDNTLDV 47
Cdd:pfam10111   1 SVVIPVYNGEKthWIQERILNQTFQYDPEFELIIINDGSTDKTLEE 46

pp-GalNAc-T initiates the formation of mucin-type O-linked glycans; UDP-GalNAc: polypeptide alpha-N-acetylgalactosaminyltransferases (pp-GalNAc-T) initiate the formation of mucin-type, O-linked glycans by catalyzing the transfer of alpha-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to hydroxyl groups of Ser or Thr residues of core proteins to form the Tn antigen (GalNAc-a-1-O-Ser/Thr). These enzymes are type II membrane proteins with a GT-A type catalytic domain and a lectin domain located on the lumen side of the Golgi apparatus. In human, there are 15 isozymes of pp-GalNAc-Ts, representing the largest of all glycosyltransferase families. Each isozyme has unique but partially redundant substrate specificity for glycosylation sites on acceptor proteins.

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 1562284756   4 SIIIPAYNAAKYV-ERCVESILSQDFNDL--EILIVDDGSSD 42
Cdd:cd02510     1 SVIIIFHNEALSTlLRTVHSVINRTPPELlkEIILVDDFSDK 42