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Conserved domains on  [gi|1590333022|ref|WP_131654606|]
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MULTISPECIES: benzoate 1,2-dioxygenase small subunit [Pseudomonas]

Protein Classification

benzoate 1,2-dioxygenase small subunit( domain architecture ID 10799071)

benzoate 1,2-dioxygenase catalyzes degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB); the beta subunit may be responsible for the substrate specificity of the enzyme

CATH:  3.10.450.50
EC:  1.14.12.10
Gene Ontology:  GO:0006725
SCOP:  4001024|3000472

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
benzo_1_2_benB TIGR03232
benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to ...
 8-162 1.76e-102

benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to the larger family of aromatic ring-hydroxylating dioxygenases. Members of this family should all act on benzoate, but several have additional known activities on various benozate analogs. Some members actually may be named more suitably according to such alternate an activity, such as 2-chlorobenzoate 1,2-dioxygenase (1.14.12.13).


:

Pssm-ID: 132276  Cd Length: 155  Bit Score: 290.56  E-value: 1.76e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   8 VRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATMPDTR 87
Cdd:TIGR03232   1 IQAFLYREARLLDDEQWDDWLECYRADASFWMPAWDDDDQLTEDPQSEISLIYYPNRQGLEDRVFRIKTERSSATVPDTR 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1590333022  88 TSHNLSNIEIVEEADGLCHVRFNWHTLSFRYKTTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYHI 162
Cdd:TIGR03232  81 TSHNISNVEIEEQDGDVITVRFNWHTLSFRYKTTDSYFGMSRYTIDFSGESPKIKSKYVVLKNDYINQVIDIYHI 155
 
Name Accession Description Interval E-value
benzo_1_2_benB TIGR03232
benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to ...
 8-162 1.76e-102

benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to the larger family of aromatic ring-hydroxylating dioxygenases. Members of this family should all act on benzoate, but several have additional known activities on various benozate analogs. Some members actually may be named more suitably according to such alternate an activity, such as 2-chlorobenzoate 1,2-dioxygenase (1.14.12.13).


Pssm-ID: 132276  Cd Length: 155  Bit Score: 290.56  E-value: 1.76e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   8 VRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATMPDTR 87
Cdd:TIGR03232   1 IQAFLYREARLLDDEQWDDWLECYRADASFWMPAWDDDDQLTEDPQSEISLIYYPNRQGLEDRVFRIKTERSSATVPDTR 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1590333022  88 TSHNLSNIEIVEEADGLCHVRFNWHTLSFRYKTTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYHI 162
Cdd:TIGR03232  81 TSHNISNVEIEEQDGDVITVRFNWHTLSFRYKTTDSYFGMSRYTIDFSGESPKIKSKYVVLKNDYINQVIDIYHI 155
ring_hydroxylating_dioxygenases_beta cd00667
Ring hydroxylating dioxygenase beta subunit. This subunit has a similar structure to NTF-2, ...
 4-161 1.06e-59

Ring hydroxylating dioxygenase beta subunit. This subunit has a similar structure to NTF-2, Ketosteroid isomerase and scytalone dehydratase.The degradation of aromatic compounds by aerobic bacteria frequently begins with the dihydroxylation of the substrate by nonheme iron-containing dioxygenases. These enzymes consist of two or three soluble proteins that interact to form an electron-transport chain that transfers electrons from reduced nucleotides (NADH) via flavin and [2Fe-2S] redox centers to a terminal dioxygenase. Aromatic-ring-hydroxylating dioxygenases oxidize aromatic hydrocarbons and related compounds to cis-arene diols. These enzymes utilize a mononuclear non-heme iron center to catalyze the addition of dioxygen to their respective substrates. The active site of these enzymes however is in the alpha sub-unit. No functional role has been attributed to the beta sub-unit except for a structural role.


Pssm-ID: 238357  Cd Length: 160  Bit Score: 182.46  E-value: 1.06e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATM 83
Cdd:cd00667     2 LQAEVEQFLYREARLLDDRRWDEWLALFAEDCHYWVPARENRERRDEDPGLELSAIYDDDRRMLEDRVVRLRTGRAWSED 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1590333022  84 PDTRTSHNLSNIEIVEEADGLCHVRFNWHTLSFRYK-TTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYH 161
Cdd:cd00667    82 PPSRTRHLVSNVRVLEGDGGEIEVRSNFVVVRTRLDgESDVFAGGRYDDLRRSEDGLRIASRRVVLDNDRIPTVNLSPF 160
Ring_hydroxyl_B pfam00866
Ring hydroxylating beta subunit; This subunit has a similar structure to NTF-2 and scytalone ...
 14-155 4.47e-55

Ring hydroxylating beta subunit; This subunit has a similar structure to NTF-2 and scytalone dehydratase.


Pssm-ID: 425916  Cd Length: 144  Bit Score: 170.17  E-value: 4.47e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022  14 REARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATMPDTRTSHNLS 93
Cdd:pfam00866   1 REARLLDDRDWDAWLALLAEDIHYWMPQREDRQRRDRDPQREESAIFDDDRAGLEDRVFRIRTGRAWAEDPPSRTRHLVS 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1590333022  94 NIEIVE-EADGLCHVRFNWHTLSFRYK-TTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQ 155
Cdd:pfam00866  81 NVRVEEtEADGELEVRSNFIVYRNRLErQVDSFAGRRTDVLRRSGDGFKIARRTILLDNSVIPS 144
HcaF COG5517
3-phenylpropionate/cinnamic acid dioxygenase, small subunit [Secondary metabolites ...
 4-162 1.33e-53

3-phenylpropionate/cinnamic acid dioxygenase, small subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 444268  Cd Length: 162  Bit Score: 166.94  E-value: 1.33e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTlteDPQSEISLIwYGNRGGLEDRIFRIKTERSSATM 83
Cdd:COG5517     7 LRAEVEQFLYREARLLDERRFDEWLALFTEDGHYWVPARENRDT---DPGLPLSLI-YDDRAMLEDRVARLRTGNAWAED 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022  84 PDTRTSHNLSNIEIVEEADGLCHVRFNWHTLSFRY-KTTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYHI 162
Cdd:COG5517    83 PPSRTRHLVSNVRVEETDGGEIEVRSNFLVYRTRRdGQTDLFVGRYEDRLRRTGGGLRIARRRVVLDNSVIPTKNLSYPL 162
PRK10069 PRK10069
3-phenylpropionate/cinnamic acid dioxygenase subunit beta;
4-117 3.51e-14

3-phenylpropionate/cinnamic acid dioxygenase subunit beta;


Pssm-ID: 236647  Cd Length: 183  Bit Score: 66.59  E-value: 3.51e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMP-----SWDDNDTlTEDPQSEISLIWYG-NRGGLEDRIFRIKTE 77
Cdd:PRK10069   18 LHHEISQFLYREARLLDEWRYDDWLALLAEDIHYTMPmrttvNAQRRDR-REGVQTPPTMAWFDdNKDQLERRVARLETG 96

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1590333022  78 RSSATMPDTRTSHNLSNIEIvEEADGLCHVRFNWHTLSFR 117
Cdd:PRK10069   97 MAWAEEPPSRLRHLITNVRV-EETDIPDEFAVRSNFLLYR 135
 
Name Accession Description Interval E-value
benzo_1_2_benB TIGR03232
benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to ...
 8-162 1.76e-102

benzoate 1,2-dioxygenase, small subunit; Benzoate 1,2-dioxygenase (EC 1.14.12.10) belongs to the larger family of aromatic ring-hydroxylating dioxygenases. Members of this family should all act on benzoate, but several have additional known activities on various benozate analogs. Some members actually may be named more suitably according to such alternate an activity, such as 2-chlorobenzoate 1,2-dioxygenase (1.14.12.13).


Pssm-ID: 132276  Cd Length: 155  Bit Score: 290.56  E-value: 1.76e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   8 VRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATMPDTR 87
Cdd:TIGR03232   1 IQAFLYREARLLDDEQWDDWLECYRADASFWMPAWDDDDQLTEDPQSEISLIYYPNRQGLEDRVFRIKTERSSATVPDTR 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1590333022  88 TSHNLSNIEIVEEADGLCHVRFNWHTLSFRYKTTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYHI 162
Cdd:TIGR03232  81 TSHNISNVEIEEQDGDVITVRFNWHTLSFRYKTTDSYFGMSRYTIDFSGESPKIKSKYVVLKNDYINQVIDIYHI 155
ring_hydroxylating_dioxygenases_beta cd00667
Ring hydroxylating dioxygenase beta subunit. This subunit has a similar structure to NTF-2, ...
 4-161 1.06e-59

Ring hydroxylating dioxygenase beta subunit. This subunit has a similar structure to NTF-2, Ketosteroid isomerase and scytalone dehydratase.The degradation of aromatic compounds by aerobic bacteria frequently begins with the dihydroxylation of the substrate by nonheme iron-containing dioxygenases. These enzymes consist of two or three soluble proteins that interact to form an electron-transport chain that transfers electrons from reduced nucleotides (NADH) via flavin and [2Fe-2S] redox centers to a terminal dioxygenase. Aromatic-ring-hydroxylating dioxygenases oxidize aromatic hydrocarbons and related compounds to cis-arene diols. These enzymes utilize a mononuclear non-heme iron center to catalyze the addition of dioxygen to their respective substrates. The active site of these enzymes however is in the alpha sub-unit. No functional role has been attributed to the beta sub-unit except for a structural role.


Pssm-ID: 238357  Cd Length: 160  Bit Score: 182.46  E-value: 1.06e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATM 83
Cdd:cd00667     2 LQAEVEQFLYREARLLDDRRWDEWLALFAEDCHYWVPARENRERRDEDPGLELSAIYDDDRRMLEDRVVRLRTGRAWSED 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1590333022  84 PDTRTSHNLSNIEIVEEADGLCHVRFNWHTLSFRYK-TTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYH 161
Cdd:cd00667    82 PPSRTRHLVSNVRVLEGDGGEIEVRSNFVVVRTRLDgESDVFAGGRYDDLRRSEDGLRIASRRVVLDNDRIPTVNLSPF 160
Ring_hydroxyl_B pfam00866
Ring hydroxylating beta subunit; This subunit has a similar structure to NTF-2 and scytalone ...
 14-155 4.47e-55

Ring hydroxylating beta subunit; This subunit has a similar structure to NTF-2 and scytalone dehydratase.


Pssm-ID: 425916  Cd Length: 144  Bit Score: 170.17  E-value: 4.47e-55
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022  14 REARYLDDGQWDQWLELYASDATYWMPSWDDNDTLTEDPQSEISLIWYGNRGGLEDRIFRIKTERSSATMPDTRTSHNLS 93
Cdd:pfam00866   1 REARLLDDRDWDAWLALLAEDIHYWMPQREDRQRRDRDPQREESAIFDDDRAGLEDRVFRIRTGRAWAEDPPSRTRHLVS 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1590333022  94 NIEIVE-EADGLCHVRFNWHTLSFRYK-TTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQ 155
Cdd:pfam00866  81 NVRVEEtEADGELEVRSNFIVYRNRLErQVDSFAGRRTDVLRRSGDGFKIARRTILLDNSVIPS 144
HcaF COG5517
3-phenylpropionate/cinnamic acid dioxygenase, small subunit [Secondary metabolites ...
 4-162 1.33e-53

3-phenylpropionate/cinnamic acid dioxygenase, small subunit [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 444268  Cd Length: 162  Bit Score: 166.94  E-value: 1.33e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMPSWDDNDTlteDPQSEISLIwYGNRGGLEDRIFRIKTERSSATM 83
Cdd:COG5517     7 LRAEVEQFLYREARLLDERRFDEWLALFTEDGHYWVPARENRDT---DPGLPLSLI-YDDRAMLEDRVARLRTGNAWAED 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022  84 PDTRTSHNLSNIEIVEEADGLCHVRFNWHTLSFRY-KTTDSYFGTSFYTLDMRGPQPLIKAKKVVLKNDYVRQVIDIYHI 162
Cdd:COG5517    83 PPSRTRHLVSNVRVEETDGGEIEVRSNFLVYRTRRdGQTDLFVGRYEDRLRRTGGGLRIARRRVVLDNSVIPTKNLSYPL 162
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 8-148 2.87e-18

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 75.63  E-value: 2.87e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   8 VRDFLYREARYLDDgQWDQWLELYASDATYWMPSWDDNDTLTEDPqseisliwygnRGGLEDRIFRIKTERSsatmpdtR 87
Cdd:cd00531     1 AEQFLYRYARLLDA-GDREWLALLYADDAYFEPPGGDGLIYPDDG-----------REAIEDRVRRLPFGPS-------R 61

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1590333022  88 TSHNLSNIEIVEEADGLCHVRFNWHTLSFRYK-TTDSYFGTSFYTLDMRGPQPLIKAKKVVL 148
Cdd:cd00531    62 TRHLVSNVDVQPGDDGEGVVVSVFGVLRTRGDgEQDVFAGGQTFVLRPQGGGGKIANRRFRL 123
PRK10069 PRK10069
3-phenylpropionate/cinnamic acid dioxygenase subunit beta;
4-117 3.51e-14

3-phenylpropionate/cinnamic acid dioxygenase subunit beta;


Pssm-ID: 236647  Cd Length: 183  Bit Score: 66.59  E-value: 3.51e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1590333022   4 THDIVRDFLYREARYLDDGQWDQWLELYASDATYWMP-----SWDDNDTlTEDPQSEISLIWYG-NRGGLEDRIFRIKTE 77
Cdd:PRK10069   18 LHHEISQFLYREARLLDEWRYDDWLALLAEDIHYTMPmrttvNAQRRDR-REGVQTPPTMAWFDdNKDQLERRVARLETG 96

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1590333022  78 RSSATMPDTRTSHNLSNIEIvEEADGLCHVRFNWHTLSFR 117
Cdd:PRK10069   97 MAWAEEPPSRLRHLITNVRV-EETDIPDEFAVRSNFLLYR 135
SnoaL_4 pfam13577
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 8-43 5.26e-04

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 433323 [Multi-domain]  Cd Length: 125  Bit Score: 38.08  E-value: 5.26e-04
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 1590333022   8 VRDFLYREARYLDDGQWDQWLELYASDATYWMPSWD 43
Cdd:pfam13577   9 IRDLVARYGRALDTGDWDELAALFTEDAVFDYGGLG 44
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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