MULTISPECIES: plasmid mobilization relaxosome protein MobC [Duncaniella]
Protein Classification
VirD1 and MobC domain-containing protein( domain architecture ID 11887388)
VirD1 and MobC domain-containing protein
List of domain hits
| Name | Accession | Description | Interval | E-value | ||
| MobC | pfam05713 | Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ... |
71-115 | 5.81e-06 | ||
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA. : Pssm-ID: 428600 Cd Length: 45 Bit Score: 40.54 E-value: 5.81e-06
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| VirD1 super family | cl27005 | T-DNA border endonuclease VirD1; This family consists of several T-DNA border endonuclease ... |
22-90 | 1.64e-03 | ||
T-DNA border endonuclease VirD1; This family consists of several T-DNA border endonuclease VirD1 proteins which appear to be found exclusively in Agrobacterium species. Agrobacterium, a plant pathogen, is capable to stably transform the plant cell with a segment of its own DNA called T-DNA (transferred DNA). This process depends, among others, on the specialized bacterial virulence proteins VirD1 and VirD2 that excise the T-DNA from its adjacent sequences. VirD1 is thought to interact with VirD2 in this process. The actual alignment was detected with superfamily member PRK13858: Pssm-ID: 452701 Cd Length: 147 Bit Score: 35.94 E-value: 1.64e-03
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| Name | Accession | Description | Interval | E-value | ||
| MobC | pfam05713 | Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ... |
71-115 | 5.81e-06 | ||
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA. Pssm-ID: 428600 Cd Length: 45 Bit Score: 40.54 E-value: 5.81e-06
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| PRK13858 | PRK13858 | T-DNA border endonuclease VirD1; |
22-90 | 1.64e-03 | ||
T-DNA border endonuclease VirD1; Pssm-ID: 237532 Cd Length: 147 Bit Score: 35.94 E-value: 1.64e-03
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| Name | Accession | Description | Interval | E-value | ||
| MobC | pfam05713 | Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, ... |
71-115 | 5.81e-06 | ||
Bacterial mobilization protein (MobC); This family consists of several bacterial MobC-like, mobilization proteins. MobC proteins belong to the group of relaxases. Together with MobA and MobB they bind to a single cis-active site of a mobilising plasmid, the origin of transfer (oriT) region. The absence of MobC has several different effects on oriT DNA. Site- and strand-specific nicking by MobA protein is severely reduced, accounting for the lower frequency of mobilization. The localized DNA strand separation required for this nicking is less affected, but becomes more sensitive to the level of active DNA gyrase in the cell. In addition, strand separation is not efficiently extended through the region containing the nick site. These effects suggest a model in which MobC acts as a molecular wedge for the relaxosome-induced melting of oriT DNA. The effect of MobC on strand separation may be partially complemented by the helical distortion induced by supercoiling. However, MobC extends the melted region through the nick site, thus providing the single-stranded substrate required for cleavage by MobA. Pssm-ID: 428600 Cd Length: 45 Bit Score: 40.54 E-value: 5.81e-06
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| PRK13858 | PRK13858 | T-DNA border endonuclease VirD1; |
22-90 | 1.64e-03 | ||
T-DNA border endonuclease VirD1; Pssm-ID: 237532 Cd Length: 147 Bit Score: 35.94 E-value: 1.64e-03
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