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Conserved domains on  [gi|1998153589|ref|WP_206511648|]
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MULTISPECIES: BtrH N-terminal domain-containing protein [Nocardiaceae]

Protein Classification

BtrH N-terminal domain-containing protein( domain architecture ID 11233448)

BtrH N-terminal domain-containing protein similar to the N-terminal domain of Bacillus circulans butirosin biosynthesis protein H (BtrH), an aminoglycoside acyltransferase that attaches the (S)-4-amino-2-hydroxybutyrate side chain from the acyl carrier protein BtrI to the aminoglycoside ribostamycin in the biosynthetic pathway of butirosin; also contains a DUF4872 domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
BtrH_N pfam14399
Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier ...
 17-150 2.50e-47

Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier protein:aminoglycoside acyltransferase BtrH. Alternatively it can be referred to as butirosin biosynthesis protein H. BtrH transfers the unique (S)-4-amino-2-hydroxybutyrate (AHBA) side chain, which protects the antibiotic butirosin from several common resistance mechanisms. Butirosin, an aminoglycoside antibiotic produced by Bacillus circulans, exhibits improved antibiotic properties over its parent molecule and retains bactericidal activity toward many aminoglycoside-resistant strains. Butirosin is unique in carrying the AHBA side-chain. BtrH transfers the AHBA from the acyl carrier protein BtrI to the parent aminoglycoside ribostamycin as a gamma-glutamylated dipeptide.


:

Pssm-ID: 433933  Cd Length: 134  Bit Score: 156.62  E-value: 2.50e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  17 HCGSGAMRDLLHWHGLGWdgppSEGLTFTLGGALGLSYLRSNALVPPLYLVGRGA--DFEVDLPRRLGGQVQVLTTDDPE 94
Cdd:pfam14399   1 HCESGALADLLAHAGLPL----SEPMAFGLGGGLGFVYLPFKKLNPLPLIGGRTKpgDLFKRLAKRLGIKVKRRTTSDPD 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1998153589  95 EGWSWVRDEIDSGRPSLVWGDIAELPYLRVQM--QMSRHDIVVIGYDEDAGVAFVVDN 150
Cdd:pfam14399  77 EAWAALDAELDAGRPVGLQVDVYYLPYFPKEYrfHFNAHNLVVYGYDDEGGVYLVSDP 134
DUF4872 pfam16169
Domain of unknown function (DUF4872); Members of this family are often found in the gene ...
 161-342 1.72e-44

Domain of unknown function (DUF4872); Members of this family are often found in the gene neighbourhood, or fused to, non-ribosomal peptide synthetases.


:

Pssm-ID: 435184  Cd Length: 173  Bit Score: 150.83  E-value: 1.72e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 161 DSLARARSSTSFPQPTRHCTYRITWPSALPSIGGTAAAAFRQSADNLRQP----SGARIANLTAAsgaeglaaaealaaD 236
Cdd:pfam16169   1 DALARARFAKGFPAPKGHMYYPLAIPDKLPDLAKAIRKAIKKNARAMLNPpvpnAGVRGIRKLAK--------------D 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 237 VHTWAD-LPDDELEILLFGLGAFIEKAGTGGGLFRRLLAEGCAEIARLTGDLATADLARVADRCAQAWTETARAGIRRGA 315
Cdd:pfam16169  67 IRKWPEkFGADDLELYLRQLVRMQEEIGTGGGGFRFLYADFLQEAAELLGDPALAELSELMTEAADAWRDFALLAARAGK 146

                         170       180
                  ....*....|....*....|....*..
gi 1998153589 316 DVRARLDAVALAAGRLPKGETDLVDAL 342
Cdd:pfam16169 147 GREADLAEAADALEELAELEEEAFTAL 173
 
Name Accession Description Interval E-value
BtrH_N pfam14399
Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier ...
 17-150 2.50e-47

Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier protein:aminoglycoside acyltransferase BtrH. Alternatively it can be referred to as butirosin biosynthesis protein H. BtrH transfers the unique (S)-4-amino-2-hydroxybutyrate (AHBA) side chain, which protects the antibiotic butirosin from several common resistance mechanisms. Butirosin, an aminoglycoside antibiotic produced by Bacillus circulans, exhibits improved antibiotic properties over its parent molecule and retains bactericidal activity toward many aminoglycoside-resistant strains. Butirosin is unique in carrying the AHBA side-chain. BtrH transfers the AHBA from the acyl carrier protein BtrI to the parent aminoglycoside ribostamycin as a gamma-glutamylated dipeptide.


Pssm-ID: 433933  Cd Length: 134  Bit Score: 156.62  E-value: 2.50e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  17 HCGSGAMRDLLHWHGLGWdgppSEGLTFTLGGALGLSYLRSNALVPPLYLVGRGA--DFEVDLPRRLGGQVQVLTTDDPE 94
Cdd:pfam14399   1 HCESGALADLLAHAGLPL----SEPMAFGLGGGLGFVYLPFKKLNPLPLIGGRTKpgDLFKRLAKRLGIKVKRRTTSDPD 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1998153589  95 EGWSWVRDEIDSGRPSLVWGDIAELPYLRVQM--QMSRHDIVVIGYDEDAGVAFVVDN 150
Cdd:pfam14399  77 EAWAALDAELDAGRPVGLQVDVYYLPYFPKEYrfHFNAHNLVVYGYDDEGGVYLVSDP 134
DUF4872 pfam16169
Domain of unknown function (DUF4872); Members of this family are often found in the gene ...
 161-342 1.72e-44

Domain of unknown function (DUF4872); Members of this family are often found in the gene neighbourhood, or fused to, non-ribosomal peptide synthetases.


Pssm-ID: 435184  Cd Length: 173  Bit Score: 150.83  E-value: 1.72e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 161 DSLARARSSTSFPQPTRHCTYRITWPSALPSIGGTAAAAFRQSADNLRQP----SGARIANLTAAsgaeglaaaealaaD 236
Cdd:pfam16169   1 DALARARFAKGFPAPKGHMYYPLAIPDKLPDLAKAIRKAIKKNARAMLNPpvpnAGVRGIRKLAK--------------D 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 237 VHTWAD-LPDDELEILLFGLGAFIEKAGTGGGLFRRLLAEGCAEIARLTGDLATADLARVADRCAQAWTETARAGIRRGA 315
Cdd:pfam16169  67 IRKWPEkFGADDLELYLRQLVRMQEEIGTGGGGFRFLYADFLQEAAELLGDPALAELSELMTEAADAWRDFALLAARAGK 146

                         170       180
                  ....*....|....*....|....*..
gi 1998153589 316 DVRARLDAVALAAGRLPKGETDLVDAL 342
Cdd:pfam16169 147 GREADLAEAADALEELAELEEEAFTAL 173
Peptidase_C39A cd02549
A sub-family of peptidase family C39. Peptidase family C39 mostly contains ...
 78-172 3.36e-03

A sub-family of peptidase family C39. Peptidase family C39 mostly contains bacteriocin-processing endopeptidases from bacteria. The cysteine peptidases in family C39 cleave the "double-glycine" leader peptides from the precursors of various bacteriocins (mostly non-lantibiotic). The cleavage is mediated by the transporter as part of the secretion process. Bacteriocins are antibiotic proteins secreted by some species of bacteria that inhibit the growth of other bacterial species. The bacteriocin is synthesized as a precursor with an N-terminal leader peptide, and processing involves removal of the leader peptide by cleavage at a Gly-Gly bond, followed by translocation of the mature bacteriocin across the cytoplasmic membrane. Most endopeptidases of family C39 are N-terminal domains in larger proteins (ABC transporters) that serve both functions. The proposed protease active site is conserved in this sub-family of proteins with a single peptidase domain, which are lacking the nucleotide-binding transporter signature or have different domain architectures.


Pssm-ID: 239109 [Multi-domain]  Cd Length: 141  Bit Score: 37.39  E-value: 3.36e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  78 PRRLGGQVQVLTTDDPeegwswVRDEIDSGRPSLVWGDIAELPYLrvqmqmSRHDIVVIGYDEDaGVAFVVDNDRAEVQE 157
Cdd:cd02549    54 ARKYGLVVRPLTGLLA------LLRQLAAGHPVIVSVNLGVSITP------SGHAMVVIGYDRK-GNVYVNDPGGGRRLV 120

                          90
                  ....*....|....*
gi 1998153589 158 VPFDSLARARSSTSF 172
Cdd:cd02549   121 VSFDEFEKAWKRMGG 135
YvpB COG4990
Predicted cysteine peptidase, C39 family [General function prediction only];
81-149 5.21e-03

Predicted cysteine peptidase, C39 family [General function prediction only];


Pssm-ID: 444014 [Multi-domain]  Cd Length: 303  Bit Score: 38.25  E-value: 5.21e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  81 LGGQVQVLTtddpeeGWSW--VRDEIDSGRPSLVWG--DIAELPYLR---------VQMQMSRHDIVVIGYDEDaGVaFV 147
Cdd:COG4990   200 LPGKAVDLT------GASFedILDELASGNPVIVWTtlDFSPPSAFRswttpdgktFDFTANEHAVVVTGYDDE-GV-YV 271


                  ..
gi 1998153589 148 VD 149
Cdd:COG4990   272 ND 273
 
Name Accession Description Interval E-value
BtrH_N pfam14399
Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier ...
 17-150 2.50e-47

Butirosin biosynthesis protein H, N-terminal; BtrH_N is the N-terminus of the acyl carrier protein:aminoglycoside acyltransferase BtrH. Alternatively it can be referred to as butirosin biosynthesis protein H. BtrH transfers the unique (S)-4-amino-2-hydroxybutyrate (AHBA) side chain, which protects the antibiotic butirosin from several common resistance mechanisms. Butirosin, an aminoglycoside antibiotic produced by Bacillus circulans, exhibits improved antibiotic properties over its parent molecule and retains bactericidal activity toward many aminoglycoside-resistant strains. Butirosin is unique in carrying the AHBA side-chain. BtrH transfers the AHBA from the acyl carrier protein BtrI to the parent aminoglycoside ribostamycin as a gamma-glutamylated dipeptide.


Pssm-ID: 433933  Cd Length: 134  Bit Score: 156.62  E-value: 2.50e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  17 HCGSGAMRDLLHWHGLGWdgppSEGLTFTLGGALGLSYLRSNALVPPLYLVGRGA--DFEVDLPRRLGGQVQVLTTDDPE 94
Cdd:pfam14399   1 HCESGALADLLAHAGLPL----SEPMAFGLGGGLGFVYLPFKKLNPLPLIGGRTKpgDLFKRLAKRLGIKVKRRTTSDPD 76

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 1998153589  95 EGWSWVRDEIDSGRPSLVWGDIAELPYLRVQM--QMSRHDIVVIGYDEDAGVAFVVDN 150
Cdd:pfam14399  77 EAWAALDAELDAGRPVGLQVDVYYLPYFPKEYrfHFNAHNLVVYGYDDEGGVYLVSDP 134
DUF4872 pfam16169
Domain of unknown function (DUF4872); Members of this family are often found in the gene ...
 161-342 1.72e-44

Domain of unknown function (DUF4872); Members of this family are often found in the gene neighbourhood, or fused to, non-ribosomal peptide synthetases.


Pssm-ID: 435184  Cd Length: 173  Bit Score: 150.83  E-value: 1.72e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 161 DSLARARSSTSFPQPTRHCTYRITWPSALPSIGGTAAAAFRQSADNLRQP----SGARIANLTAAsgaeglaaaealaaD 236
Cdd:pfam16169   1 DALARARFAKGFPAPKGHMYYPLAIPDKLPDLAKAIRKAIKKNARAMLNPpvpnAGVRGIRKLAK--------------D 66

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589 237 VHTWAD-LPDDELEILLFGLGAFIEKAGTGGGLFRRLLAEGCAEIARLTGDLATADLARVADRCAQAWTETARAGIRRGA 315
Cdd:pfam16169  67 IRKWPEkFGADDLELYLRQLVRMQEEIGTGGGGFRFLYADFLQEAAELLGDPALAELSELMTEAADAWRDFALLAARAGK 146

                         170       180
                  ....*....|....*....|....*..
gi 1998153589 316 DVRARLDAVALAAGRLPKGETDLVDAL 342
Cdd:pfam16169 147 GREADLAEAADALEELAELEEEAFTAL 173
Peptidase_C39_2 pfam13529
Peptidase_C39 like family;
75-149 2.13e-04

Peptidase_C39 like family;


Pssm-ID: 379241 [Multi-domain]  Cd Length: 139  Bit Score: 40.89  E-value: 2.13e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1998153589  75 VDLPRRLGGQVQVLTTDDpeegWSWVRDEIDSGRPSLVWgdIAELPYLRVQMQMSRHDIVVIGYDEDAGVAFVVD 149
Cdd:pfam13529  70 VALAEKYGLKVTDITGSS----FDEVIRLLDAGIPVVVS--TTTFGPLNYYFTSSGHLVVIVGYDDKGDYVYVND 138
Peptidase_C39A cd02549
A sub-family of peptidase family C39. Peptidase family C39 mostly contains ...
 78-172 3.36e-03

A sub-family of peptidase family C39. Peptidase family C39 mostly contains bacteriocin-processing endopeptidases from bacteria. The cysteine peptidases in family C39 cleave the "double-glycine" leader peptides from the precursors of various bacteriocins (mostly non-lantibiotic). The cleavage is mediated by the transporter as part of the secretion process. Bacteriocins are antibiotic proteins secreted by some species of bacteria that inhibit the growth of other bacterial species. The bacteriocin is synthesized as a precursor with an N-terminal leader peptide, and processing involves removal of the leader peptide by cleavage at a Gly-Gly bond, followed by translocation of the mature bacteriocin across the cytoplasmic membrane. Most endopeptidases of family C39 are N-terminal domains in larger proteins (ABC transporters) that serve both functions. The proposed protease active site is conserved in this sub-family of proteins with a single peptidase domain, which are lacking the nucleotide-binding transporter signature or have different domain architectures.


Pssm-ID: 239109 [Multi-domain]  Cd Length: 141  Bit Score: 37.39  E-value: 3.36e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  78 PRRLGGQVQVLTTDDPeegwswVRDEIDSGRPSLVWGDIAELPYLrvqmqmSRHDIVVIGYDEDaGVAFVVDNDRAEVQE 157
Cdd:cd02549    54 ARKYGLVVRPLTGLLA------LLRQLAAGHPVIVSVNLGVSITP------SGHAMVVIGYDRK-GNVYVNDPGGGRRLV 120

                          90
                  ....*....|....*
gi 1998153589 158 VPFDSLARARSSTSF 172
Cdd:cd02549   121 VSFDEFEKAWKRMGG 135
YvpB COG4990
Predicted cysteine peptidase, C39 family [General function prediction only];
81-149 5.21e-03

Predicted cysteine peptidase, C39 family [General function prediction only];


Pssm-ID: 444014 [Multi-domain]  Cd Length: 303  Bit Score: 38.25  E-value: 5.21e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1998153589  81 LGGQVQVLTtddpeeGWSW--VRDEIDSGRPSLVWG--DIAELPYLR---------VQMQMSRHDIVVIGYDEDaGVaFV 147
Cdd:COG4990   200 LPGKAVDLT------GASFedILDELASGNPVIVWTtlDFSPPSAFRswttpdgktFDFTANEHAVVVTGYDDE-GV-YV 271


                  ..
gi 1998153589 148 VD 149
Cdd:COG4990   272 ND 273
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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