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Conserved domains on  [gi|2052573654|ref|WP_216056680|]
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MULTISPECIES: HIT domain-containing protein [Psychrosphaera]

Protein Classification

HIT family protein( domain architecture ID 694)

HIT (Histidine triad) family protein, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), is part of a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides

CATH:  3.30.428.10
Gene Ontology:  GO:1904047
PubMed:  1472710|9323207|12504684|12119013
SCOP:  3000223

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
HIT_like super family cl00228
HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH ...
 7-99 5.82e-21

HIT family: HIT (Histidine triad) proteins, named for a motif related to the sequence HxHxH/Qxx (x, a hydrophobic amino acid), are a superfamily of nucleotide hydrolases and transferases, which act on the alpha-phosphate of ribonucleotides. On the basis of sequence, substrate specificity, structure, evolution and mechanism, HIT proteins are classified in the literacture into three major branches: the Hint branch, which consists of adenosine 5' -monophosphoramide hydrolases, the Fhit branch, that consists of diadenosine polyphosphate hydrolases, and the GalT branch consisting of specific nucloside monophosphate transferases. Further sequence analysis reveals several new closely related, yet uncharacterized subgroups.


The actual alignment was detected with superfamily member pfam01230:

Pssm-ID: 469672 [Multi-domain]  Cd Length: 98  Bit Score: 81.20  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2052573654   7 RIEKDSIFVKELPLCQLRLQNDSRYPWFVLLPRVNELTEVHQLSEHEQMQLMKESALVSKALTATTQCK-----KINVAN 81
Cdd:pfam01230   1 RGEIPSTVVYEDDLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADgyrivINNGAH 80

                          90
                  ....*....|....*...
gi 2052573654  82 LGNVVAQLHWHVVARFEH 99
Cdd:pfam01230  81 AGQSVPHLHIHVIPRRKH 98
 
Name Accession Description Interval E-value
HIT pfam01230
HIT domain;
7-99 5.82e-21

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 81.20  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2052573654   7 RIEKDSIFVKELPLCQLRLQNDSRYPWFVLLPRVNELTEVHQLSEHEQMQLMKESALVSKALTATTQCK-----KINVAN 81
Cdd:pfam01230   1 RGEIPSTVVYEDDLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADgyrivINNGAH 80

                          90
                  ....*....|....*...
gi 2052573654  82 LGNVVAQLHWHVVARFEH 99
Cdd:pfam01230  81 AGQSVPHLHIHVIPRRKH 98
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 40-108 2.29e-11

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 57.27  E-value: 2.29e-11
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2052573654  40 VNELTEvhqLSEHEQMQLMKESALVSKALTATTQCKKINVANL-----GNVVAQLHWHVVARFEHDQTWPGPIW 108
Cdd:COG0537    46 VASLFD---LTPEELAELMRLAQKVAKALRKALGPDGFNLGINngeaaGQTVPHLHVHVIPRYEGDDNFMPVIG 116
 
Name Accession Description Interval E-value
HIT pfam01230
HIT domain;
7-99 5.82e-21

HIT domain;


Pssm-ID: 395984 [Multi-domain]  Cd Length: 98  Bit Score: 81.20  E-value: 5.82e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2052573654   7 RIEKDSIFVKELPLCQLRLQNDSRYPWFVLLPRVNELTEVHQLSEHEQMQLMKESALVSKALTATTQCK-----KINVAN 81
Cdd:pfam01230   1 RGEIPSTVVYEDDLVLAFLDIDPQAPGHILVIPKKHIRELHDLTPEELGDLMSVAQKVARALGKVFKADgyrivINNGAH 80

                          90
                  ....*....|....*...
gi 2052573654  82 LGNVVAQLHWHVVARFEH 99
Cdd:pfam01230  81 AGQSVPHLHIHVIPRRKH 98
HinT COG0537
Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide ...
 40-108 2.29e-11

Purine nucleoside phosphoramidase/Ap4A hydrolase, histidine triade (HIT) family [Nucleotide transport and metabolism, General function prediction only];


Pssm-ID: 440303 [Multi-domain]  Cd Length: 133  Bit Score: 57.27  E-value: 2.29e-11
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2052573654  40 VNELTEvhqLSEHEQMQLMKESALVSKALTATTQCKKINVANL-----GNVVAQLHWHVVARFEHDQTWPGPIW 108
Cdd:COG0537    46 VASLFD---LTPEELAELMRLAQKVAKALRKALGPDGFNLGINngeaaGQTVPHLHVHVIPRYEGDDNFMPVIG 116
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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