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Conserved domains on  [gi|2153709903|ref|WP_229128912|]
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MULTISPECIES: cysteine hydrolase family protein [Bacillus]

Protein Classification

cysteine hydrolase family protein( domain architecture ID 10003554)

cysteine hydrolase family protein related to isochorismatase and nicotinamidase; catalyzes the hydrolysis of a chemical bond using an active site cysteinyl residue

CATH:  3.40.50.850
EC:  3.-.-.-
Gene Ontology:  GO:0016787

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PncA COG1335
Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction ...
 10-153 7.71e-35

Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction only]; Nicotinamidase-related amidase is part of the Pathway/BioSystem: Pyrimidine degradation


:

Pssm-ID: 440946 [Multi-domain]  Cd Length: 169  Bit Score: 119.62  E-value: 7.71e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  10 RNMTEHLLALKEHAAKKKWPIIYINDHF---GIWQADIAAIIKKC-KNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTAL 85
Cdd:COG1335    23 DAVVANIARLLAAARAAGVPVIHTRDWHppdGSEFAEFDLWPPHCvPGTPGAELVPELAPLPGDPVVDKTRYSAFYGTDL 102

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2153709903  86 ETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMMENvLFANIT 153
Cdd:COG1335   103 DELLRERGIDTLVVAGLATDVCVLSTARDALDLGYEVTVVEDACASRDPEAHEAALARLRA-AGATVV 169
 
Name Accession Description Interval E-value
PncA COG1335
Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction ...
 10-153 7.71e-35

Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction only]; Nicotinamidase-related amidase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440946 [Multi-domain]  Cd Length: 169  Bit Score: 119.62  E-value: 7.71e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  10 RNMTEHLLALKEHAAKKKWPIIYINDHF---GIWQADIAAIIKKC-KNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTAL 85
Cdd:COG1335    23 DAVVANIARLLAAARAAGVPVIHTRDWHppdGSEFAEFDLWPPHCvPGTPGAELVPELAPLPGDPVVDKTRYSAFYGTDL 102

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2153709903  86 ETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMMENvLFANIT 153
Cdd:COG1335   103 DELLRERGIDTLVVAGLATDVCVLSTARDALDLGYEVTVVEDACASRDPEAHEAALARLRA-AGATVV 169
cysteine_hydrolases cd00431
Cysteine hydrolases; This family contains amidohydrolases, like CSHase (N-carbamoylsarcosine ...
 2-145 9.96e-35

Cysteine hydrolases; This family contains amidohydrolases, like CSHase (N-carbamoylsarcosine amidohydrolase), involved in creatine metabolism and nicotinamidase, converting nicotinamide to nicotinic acid and ammonia in the pyridine nucleotide cycle. It also contains isochorismatase, an enzyme that catalyzes the conversion of isochorismate to 2,3-dihydroxybenzoate and pyruvate, via the hydrolysis of the vinyl ether bond, and other related enzymes with unknown function.


Pssm-ID: 238245 [Multi-domain]  Cd Length: 161  Bit Score: 118.91  E-value: 9.96e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903   2 GDVLSKKTRNMTEHLLALKEHAAKKKWPIIYINDHFGIWQADIAAIIKK---CKNDRSEYIIDRLAPNNNDYFLIKPKHS 78
Cdd:cd00431    15 GGLLLPGADELVPNINRLLAAARAAGIPVIFTRDWHPPDDPEFAELLWPphcVKGTEGAELVPELAPLPDDLVIEKTRYS 94

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2153709903  79 AFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMME 145
Cdd:cd00431    95 AFYGTDLDELLRERGIDTLVVCGIATDICVLATARDALDLGYRVIVVEDACATRDEEDHEAALERLA 161
Isochorismatase pfam00857
Isochorismatase family; This family are hydrolase enzymes.
 9-155 2.55e-30

Isochorismatase family; This family are hydrolase enzymes.


Pssm-ID: 376404 [Multi-domain]  Cd Length: 173  Bit Score: 108.26  E-value: 2.55e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903   9 TRNMTEHLLALKEHAAKKKWPIIYINDHFGIWQADIAAIIKKC----KNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTA 84
Cdd:pfam00857  23 IAAILENINRLLKAARKAGIPVIFTRQVPEPDDADFALKDRPSpafpPGTTGAELVPELAPLPGDLVVDKTRFSAFAGTD 102

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2153709903  85 LETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMMENvLFANITTA 155
Cdd:pfam00857 103 LDEILRELGIDTLVLAGVATDVCVLSTARDALDRGYEVVVVSDACASLSPEAHDAALERLAQ-RGAEVTTT 172
PRK11440 PRK11440
putative hydrolase; Provisional
 63-142 2.51e-07

putative hydrolase; Provisional


Pssm-ID: 183137  Cd Length: 188  Bit Score: 48.19  E-value: 2.51e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  63 LAPNNNDYFLIKPKHSAFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALT 142
Cdd:PRK11440   90 LGKTDSDIEVTKRQWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHQNSMN 169
 
Name Accession Description Interval E-value
PncA COG1335
Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction ...
 10-153 7.71e-35

Nicotinamidase-related amidase [Coenzyme transport and metabolism, General function prediction only]; Nicotinamidase-related amidase is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 440946 [Multi-domain]  Cd Length: 169  Bit Score: 119.62  E-value: 7.71e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  10 RNMTEHLLALKEHAAKKKWPIIYINDHF---GIWQADIAAIIKKC-KNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTAL 85
Cdd:COG1335    23 DAVVANIARLLAAARAAGVPVIHTRDWHppdGSEFAEFDLWPPHCvPGTPGAELVPELAPLPGDPVVDKTRYSAFYGTDL 102

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2153709903  86 ETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMMENvLFANIT 153
Cdd:COG1335   103 DELLRERGIDTLVVAGLATDVCVLSTARDALDLGYEVTVVEDACASRDPEAHEAALARLRA-AGATVV 169
cysteine_hydrolases cd00431
Cysteine hydrolases; This family contains amidohydrolases, like CSHase (N-carbamoylsarcosine ...
 2-145 9.96e-35

Cysteine hydrolases; This family contains amidohydrolases, like CSHase (N-carbamoylsarcosine amidohydrolase), involved in creatine metabolism and nicotinamidase, converting nicotinamide to nicotinic acid and ammonia in the pyridine nucleotide cycle. It also contains isochorismatase, an enzyme that catalyzes the conversion of isochorismate to 2,3-dihydroxybenzoate and pyruvate, via the hydrolysis of the vinyl ether bond, and other related enzymes with unknown function.


Pssm-ID: 238245 [Multi-domain]  Cd Length: 161  Bit Score: 118.91  E-value: 9.96e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903   2 GDVLSKKTRNMTEHLLALKEHAAKKKWPIIYINDHFGIWQADIAAIIKK---CKNDRSEYIIDRLAPNNNDYFLIKPKHS 78
Cdd:cd00431    15 GGLLLPGADELVPNINRLLAAARAAGIPVIFTRDWHPPDDPEFAELLWPphcVKGTEGAELVPELAPLPDDLVIEKTRYS 94

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2153709903  79 AFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMME 145
Cdd:cd00431    95 AFYGTDLDELLRERGIDTLVVCGIATDICVLATARDALDLGYRVIVVEDACATRDEEDHEAALERLA 161
Isochorismatase pfam00857
Isochorismatase family; This family are hydrolase enzymes.
 9-155 2.55e-30

Isochorismatase family; This family are hydrolase enzymes.


Pssm-ID: 376404 [Multi-domain]  Cd Length: 173  Bit Score: 108.26  E-value: 2.55e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903   9 TRNMTEHLLALKEHAAKKKWPIIYINDHFGIWQADIAAIIKKC----KNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTA 84
Cdd:pfam00857  23 IAAILENINRLLKAARKAGIPVIFTRQVPEPDDADFALKDRPSpafpPGTTGAELVPELAPLPGDLVVDKTRFSAFAGTD 102

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2153709903  85 LETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMMENvLFANITTA 155
Cdd:pfam00857 103 LDEILRELGIDTLVLAGVATDVCVLSTARDALDRGYEVVVVSDACASLSPEAHDAALERLAQ-RGAEVTTT 172
EntB1 COG1535
Isochorismate hydrolase [Secondary metabolites biosynthesis, transport and catabolism];
14-160 1.04e-26

Isochorismate hydrolase [Secondary metabolites biosynthesis, transport and catabolism];


Pssm-ID: 441144 [Multi-domain]  Cd Length: 204  Bit Score: 99.54  E-value: 1.04e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  14 EHLLALKEHAAKKKWPIIY--------------INDhfgIWQADIAAiikkckNDRSEYIIDRLAPNNNDYFLIKPKHSA 79
Cdd:COG1535    47 ANIARLRDACRAAGIPVVYtaqpgdqtpedrglLND---FWGPGLTA------GPEGQEIVDELAPAPGDTVLTKWRYSA 117

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  80 FYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALtmmENV--LFANITTAKE 157
Cdd:COG1535   118 FQRTDLEERLRELGRDQLIITGVYAHIGCLATAVDAFMRDIQPFVVADAVADFSREEHRMAL---EYVagRCGVVVTTDE 194


                  ...
gi 2153709903 158 ITA 160
Cdd:COG1535   195 VLE 197
isochorismatase cd01013
Isochorismatase, also known as 2,3 dihydro-2,3 dihydroxybenzoate synthase, catalyses the ...
 59-142 9.43e-14

Isochorismatase, also known as 2,3 dihydro-2,3 dihydroxybenzoate synthase, catalyses the conversion of isochorismate, in the presence of water, to 2,3-dihydroxybenzoate and pyruvate, via the hydrolysis of a vinyl ether, an uncommon reaction in biological systems. Isochorismatase is part of the phenazine biosynthesis pathway. Phenazines are antimicrobial compounds that provide the competitive advantage for certain bacteria.


Pssm-ID: 238495  Cd Length: 203  Bit Score: 65.82  E-value: 9.43e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  59 IIDRLAPNNNDYFLIKPKHSAFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNK 138
Cdd:cd01013   107 IVTELAPQPDDTVLTKWRYSAFKRSPLLERLKESGRDQLIITGVYAHIGCLSTAVDAFMRDIQPFVVADAIADFSLEEHR 186


                  ....
gi 2153709903 139 YALT 142
Cdd:cd01013   187 MALK 190
CSHase cd01015
N-carbamoylsarcosine amidohydrolase (CSHase) hydrolyzes N-carbamoylsarcosine to sarcosine, ...
 19-140 2.69e-13

N-carbamoylsarcosine amidohydrolase (CSHase) hydrolyzes N-carbamoylsarcosine to sarcosine, carbon dioxide and ammonia. CSHase is involved in one of the two alternative pathways for creatinine degradation to glycine in microorganisms.This CSHase-containing pathway degrades creatinine via N-methylhydantoin N-carbamoylsarcosine and sarcosine to glycine. Enzymes of this pathway are used in the diagnosis for renal disfunction, for determining creatinine levels in urine and serum.


Pssm-ID: 238497 [Multi-domain]  Cd Length: 179  Bit Score: 63.96  E-value: 2.69e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  19 LKEHAAKKKWPIIYIN--------DHfGIWQADIAAIIKKCKNDRSEYIIDRLAPNNNDYFLIKPKHSAFYGTALETLLN 90
Cdd:cd01015    32 LLAAARAAGVPVIHTTvvydpdgaDG-GLWARKVPAMSDLVEGSPLAAICDELAPQEDEMVLVKKYASAFFGTSLAATLT 110

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2153709903  91 ELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEK---------DNKYA 140
Cdd:cd01015   111 ARGVDTLIVAGCSTSGCIRATAVDAMQHGFRPIVVRECVGDRAPApheanlfdiDNKYG 169
nicotinamidase_related cd01014
Nicotinamidase_ related amidohydrolases. Cysteine hydrolases of unknown function that share ...
 10-131 1.92e-12

Nicotinamidase_ related amidohydrolases. Cysteine hydrolases of unknown function that share the catalytic triad with other amidohydrolases, like nicotinamidase, which converts nicotinamide to nicotinic acid and ammonia.


Pssm-ID: 238496 [Multi-domain]  Cd Length: 155  Bit Score: 61.07  E-value: 1.92e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  10 RNMTEHLLALKEHAAKKKWPIIYINDHFG----------IWQadiaaiikkckndrseyIIDRLAPNNNDYFLIKPKHSA 79
Cdd:cd01014    22 EAALENIAALIAAARAAGIPVIHVRHIDDeggsfapgseGWE-----------------IHPELAPLEGETVIEKTVPNA 84

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2153709903  80 FYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIAS 131
Cdd:cd01014    85 FYGTDLEEWLREAGIDHLVICGAMTEMCVDTTVRSAFDLGYDVTVVADACAT 136
YcaC_related cd01012
YcaC related amidohydrolases; E.coli YcaC is an homooctameric hydrolase with unknown ...
 74-145 1.57e-07

YcaC related amidohydrolases; E.coli YcaC is an homooctameric hydrolase with unknown specificity. Despite its weak sequence similarity, it is structurally related to other amidohydrolases and shares conserved active site residues with them. Multimerisation interface seems not to be conserved in all members.


Pssm-ID: 238494 [Multi-domain]  Cd Length: 157  Bit Score: 47.97  E-value: 1.57e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2153709903  74 KPKHSAFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALTMME 145
Cdd:cd01012    68 KTSFSCWEDEAFRKALKATGRKQVVLAGLETHVCVLQTALDLLEEGYEVFVVADACGSRSKEDHELALARMR 139
PRK11440 PRK11440
putative hydrolase; Provisional
 63-142 2.51e-07

putative hydrolase; Provisional


Pssm-ID: 183137  Cd Length: 188  Bit Score: 48.19  E-value: 2.51e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2153709903  63 LAPNNNDYFLIKPKHSAFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNEKDNKYALT 142
Cdd:PRK11440   90 LGKTDSDIEVTKRQWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHQNSMN 169
PLN02621 PLN02621
nicotinamidase
 60-134 3.56e-07

nicotinamidase


Pssm-ID: 178229  Cd Length: 197  Bit Score: 47.85  E-value: 3.56e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2153709903  60 IDRLAPNnnDYFLIKPKHSAFYGTALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDCIASNNE 134
Cdd:PLN02621   95 IGRVTGP--DEVVEKSTYSAFYNTRLEERLRKIGVKEVIVTGVMTNLCCETTAREAFVRGFRVFFSTDATATANE 167
nicotinamidase cd01011
Nicotinamidase/pyrazinamidase (PZase). Nicotinamidase, a ubiquitous enzyme in prokaryotes, ...
 59-115 3.37e-06

Nicotinamidase/pyrazinamidase (PZase). Nicotinamidase, a ubiquitous enzyme in prokaryotes, converts nicotinamide to nicotinic acid (niacin) and ammonia, which in turn can be recycled to make nicotinamide adenine dinucleotide (NAD). The same enzyme is also called pyrazinamidase, because in converts the tuberculosis drug pyrazinamide (PZA) into its active form pyrazinoic acid (POA).


Pssm-ID: 238493  Cd Length: 196  Bit Score: 44.95  E-value: 3.37e-06
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2153709903  59 IIDRLAPNNNDYFLIK------PKHSAFYG------TALETLLNELHVDHLIITGIAGNICVLFTANDA 115
Cdd:cd01011    91 LHPGLPVPDIDLIVRKgtnpdiDSYSAFFDndrrssTGLAEYLRERGIDRVDVVGLATDYCVKATALDA 159
PRK11609 PRK11609
bifunctional nicotinamidase/pyrazinamidase;
77-144 6.12e-06

bifunctional nicotinamidase/pyrazinamidase;


Pssm-ID: 183228  Cd Length: 212  Bit Score: 44.21  E-value: 6.12e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2153709903  77 HSAFYG------TALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHD-CIASN-NEKDNKYALTMM 144
Cdd:PRK11609  119 YSAFFDnghrqkTALDDWLREHGITELIVMGLATDYCVKFTVLDALALGYQVNVITDgCRGVNlQPQDSAHAFMEM 194
PTZ00331 PTZ00331
alpha/beta hydrolase; Provisional
 77-128 5.93e-05

alpha/beta hydrolase; Provisional


Pssm-ID: 240363  Cd Length: 212  Bit Score: 41.59  E-value: 5.93e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 2153709903  77 HSAFYG-----TALETLLNELHVDHLIITGIAGNICVLFTANDAYMREYDITIPHDC 128
Cdd:PTZ00331  124 YSAFDNdkgskTGLAQILKAHGVRRVFICGLAFDFCVLFTALDAVKLGFKVVVLEDA 180
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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