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Conserved domains on  [gi|2169866323|ref|WP_232774721|]
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glycosyltransferase family 2 protein [Brevibacillus sp. AF8]

Protein Classification

glycosyltransferase family 2 protein( domain architecture ID 10118426)

glycosyltransferase family 2 protein catalyzes the transfer of saccharide moieties from a donor to an acceptor to form glycosidic bonds

CAZY:  GT2
EC:  2.4.-.-
Gene Ontology:  GO:0016757
PubMed:  9445404|12691742
SCOP:  3000077

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-224 4.92e-48

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


:

Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 162.07  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTVYDLVDEIIIVDTGSIDRTKEIALTYGAQLFDFtWVDDFSAARNFAFAQAKMTYIFWLD 82
Cdd:cd02511     1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQR-WWDGFGAQRNFALELATNDWVLSLD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  83 ADDVLLEADRERFAALKASDNFDYDSVSMPYHLTVDE-RGKPVHYLRRNRLVKRKCNFVWHGAVHEYLAVAG-------- 153
Cdd:cd02511    80 ADERLTPELADEILALLATDDYDGYYVPRRNFFLGRWiRHGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgvgivlkg 159

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2169866323 154 DILH-SEIAITHQKNKpyTDRNLQIYVKRK-EKGevfeprDQFYFANELFDHARYEEAAEQYQL---FLDGGLGWI 224
Cdd:cd02511   160 DILHyGYKSLEEFLEK--HNRYSSLEAKDLaAKG------KKRSLLKGLLLGRPLLAFLKMYILkrgFLDGRAGFI 227
Spy super family cl27809
Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational ...
 194-288 2.92e-05

Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational modification, protein turnover, chaperones];


The actual alignment was detected with superfamily member COG3914:

Pssm-ID: 443119 [Multi-domain]  Cd Length: 658  Bit Score: 45.75  E-value: 2.92e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323 194 FYFANELFDHARYEEAAEQYQLFLDGGLGWIEdqiwACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICCRMGAFFV 273
Cdd:COG3914   116 FNLGNLLLALGRLEEALAALRRALALNPDFAE----AYLNLGEALRRLGRLEEAIAALRRALELDPDNAEALNNLGNALQ 191

                          90
                  ....*....|....*
gi 2169866323 274 EKQDFPKAIYWYRQA 288
Cdd:COG3914   192 DLGRLEEAIAAYRRA 206
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-224 4.92e-48

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 162.07  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTVYDLVDEIIIVDTGSIDRTKEIALTYGAQLFDFtWVDDFSAARNFAFAQAKMTYIFWLD 82
Cdd:cd02511     1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQR-WWDGFGAQRNFALELATNDWVLSLD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  83 ADDVLLEADRERFAALKASDNFDYDSVSMPYHLTVDE-RGKPVHYLRRNRLVKRKCNFVWHGAVHEYLAVAG-------- 153
Cdd:cd02511    80 ADERLTPELADEILALLATDDYDGYYVPRRNFFLGRWiRHGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgvgivlkg 159

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2169866323 154 DILH-SEIAITHQKNKpyTDRNLQIYVKRK-EKGevfeprDQFYFANELFDHARYEEAAEQYQL---FLDGGLGWI 224
Cdd:cd02511   160 DILHyGYKSLEEFLEK--HNRYSSLEAKDLaAKG------KKRSLLKGLLLGRPLLAFLKMYILkrgFLDGRAGFI 227
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-106 4.44e-20

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 87.06  E-value: 4.44e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   1 MFTVSLCMIVRDEEESLGRCLSTV---YDLVDEIIIVDTGSIDRTKEIALTYGAQLFDFTWVD-----DFSAARNFAFAQ 72
Cdd:COG0463     1 MPLVSVVIPTYNEEEYLEEALESLlaqTYPDFEIIVVDDGSTDGTAEILRELAAKDPRIRVIRlernrGKGAARNAGLAA 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2169866323  73 AKMTYIFWLDADDVLLEADRERFAALKASDNFDY 106
Cdd:COG0463    81 ARGDYIAFLDADDQLDPEKLEELVAALEEGPADL 114
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 5-146 1.72e-16

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 76.28  E-value: 1.72e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   5 SLCMIVRDEEESLGRCLSTV---YDLVDEIIIVDTGSIDRTKEIALTYGAQLFDF-----TWVDDFSAARNFAFAQAKMT 76
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLlnqTYPNFEIIVVDDGSTDGTVEIAEEYAKKDPRVrvirlPENRGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  77 YIFWLDADDVLLEADRERFAALKASDNFDYDSVSMPYHLTVDERGKPVHYLRRNRLVKRKCNFVWHGAVH 146
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGLNLP 150
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 3-51 3.30e-08

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 54.15  E-value: 3.30e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTV-----YDLVDEIIIVDTGSIDRTKEIALTYGA 51
Cdd:PRK13915   32 TVSVVLPALNEEETVGKVVDSIrpllmEPLVDELIVIDSGSTDATAERAAAAGA 85
Spy COG3914
Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational ...
 194-288 2.92e-05

Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 443119 [Multi-domain]  Cd Length: 658  Bit Score: 45.75  E-value: 2.92e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323 194 FYFANELFDHARYEEAAEQYQLFLDGGLGWIEdqiwACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICCRMGAFFV 273
Cdd:COG3914   116 FNLGNLLLALGRLEEALAALRRALALNPDFAE----AYLNLGEALRRLGRLEEAIAALRRALELDPDNAEALNNLGNALQ 191

                          90
                  ....*....|....*
gi 2169866323 274 EKQDFPKAIYWYRQA 288
Cdd:COG3914   192 DLGRLEEAIAAYRRA 206
PRK14720 PRK14720
transcription elongation factor GreA;
226-288 2.02e-03

transcription elongation factor GreA;


Pssm-ID: 184804 [Multi-domain]  Cd Length: 906  Bit Score: 40.14  E-value: 2.02e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2169866323 226 DQIWACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICcRMGAFFVEKQDFPKAIYWYRQA 288
Cdd:PRK14720  114 ENKLALRTLAEAYAKLNENKKLKGVWERLVKADRDNPEIV-KKLATSYEEEDKEKAITYLKKA 175
 
Name Accession Description Interval E-value
Beta4Glucosyltransferase cd02511
UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of ...
 3-224 4.92e-48

UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide; UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.


Pssm-ID: 133005 [Multi-domain]  Cd Length: 229  Bit Score: 162.07  E-value: 4.92e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTVYDLVDEIIIVDTGSIDRTKEIALTYGAQLFDFtWVDDFSAARNFAFAQAKMTYIFWLD 82
Cdd:cd02511     1 TLSVVIITKNEERNIERCLESVKWAVDEIIVVDSGSTDRTVEIAKEYGAKVYQR-WWDGFGAQRNFALELATNDWVLSLD 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  83 ADDVLLEADRERFAALKASDNFDYDSVSMPYHLTVDE-RGKPVHYLRRNRLVKRKCNFVWHGAVHEYLAVAG-------- 153
Cdd:cd02511    80 ADERLTPELADEILALLATDDYDGYYVPRRNFFLGRWiRHGGWYPDRQLRLFRRGKARFEDGRVHEQVVVDGgvgivlkg 159

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2169866323 154 DILH-SEIAITHQKNKpyTDRNLQIYVKRK-EKGevfeprDQFYFANELFDHARYEEAAEQYQL---FLDGGLGWI 224
Cdd:cd02511   160 DILHyGYKSLEEFLEK--HNRYSSLEAKDLaAKG------KKRSLLKGLLLGRPLLAFLKMYILkrgFLDGRAGFI 227
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 1-106 4.44e-20

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 87.06  E-value: 4.44e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   1 MFTVSLCMIVRDEEESLGRCLSTV---YDLVDEIIIVDTGSIDRTKEIALTYGAQLFDFTWVD-----DFSAARNFAFAQ 72
Cdd:COG0463     1 MPLVSVVIPTYNEEEYLEEALESLlaqTYPDFEIIVVDDGSTDGTAEILRELAAKDPRIRVIRlernrGKGAARNAGLAA 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 2169866323  73 AKMTYIFWLDADDVLLEADRERFAALKASDNFDY 106
Cdd:COG0463    81 ARGDYIAFLDADDQLDPEKLEELVAALEEGPADL 114
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 5-146 1.72e-16

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 76.28  E-value: 1.72e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   5 SLCMIVRDEEESLGRCLSTV---YDLVDEIIIVDTGSIDRTKEIALTYGAQLFDF-----TWVDDFSAARNFAFAQAKMT 76
Cdd:pfam00535   1 SVIIPTYNEEKYLLETLESLlnqTYPNFEIIVVDDGSTDGTVEIAEEYAKKDPRVrvirlPENRGKAGARNAGLRAATGD 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  77 YIFWLDADDVLLEADRERFAALKASDNFDYDSVSMPYHLTVDERGKPVHYLRRNRLVKRKCNFVWHGAVH 146
Cdd:pfam00535  81 YIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGLNLP 150
Glyco_tranf_GTA_type cd00761
Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a ...
 8-106 2.87e-11

Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold; Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.


Pssm-ID: 132997 [Multi-domain]  Cd Length: 156  Bit Score: 60.98  E-value: 2.87e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   8 MIVRDEEESLGRCLSTVYDL---VDEIIIVDTGSIDRTKEIALTYGAQLFDFTWVDD-----FSAARNFAFAQAKMTYIF 79
Cdd:cd00761     3 IPAYNEEPYLERCLESLLAQtypNFEVIVVDDGSTDGTLEILEEYAKKDPRVIRVINeenqgLAAARNAGLKAARGEYIL 82

                          90       100
                  ....*....|....*....|....*...
gi 2169866323  80 WLDADDVLL-EADRERFAALKASDNFDY 106
Cdd:cd00761    83 FLDADDLLLpDWLERLVAELLADPEADA 110
WcaE COG1216
Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];
1-91 5.13e-11

Glycosyltransferase, GT2 family [Carbohydrate transport and metabolism];


Pssm-ID: 440829 [Multi-domain]  Cd Length: 202  Bit Score: 61.55  E-value: 5.13e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   1 MFTVSLCMIVRDEEESLGRCLSTVY---DLVDEIIIVDTGSIDRTKEIALTYgaQLFDFTWVDD-----FSAARNFAFAQ 72
Cdd:COG1216     2 RPKVSVVIPTYNRPELLRRCLESLLaqtYPPFEVIVVDNGSTDGTAELLAAL--AFPRVRVIRNpenlgFAAARNLGLRA 79

                          90
                  ....*....|....*....
gi 2169866323  73 AKMTYIFWLDaDDVLLEAD 91
Cdd:COG1216    80 AGGDYLLFLD-DDTVVEPD 97
BcsA COG1215
Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1, ...
 3-87 2.75e-10

Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility];


Pssm-ID: 440828 [Multi-domain]  Cd Length: 303  Bit Score: 60.53  E-value: 2.75e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTVYDL-----VDEIIIVDTGSIDRTKEIALTYGAQLFDFTWVDD-----FSAARNFAFAQ 72
Cdd:COG1215    30 RVSVIIPAYNEEAVIEETLRSLLAQdypkeKLEVIVVDDGSTDETAEIARELAAEYPRVRVIERpenggKAAALNAGLKA 109

                          90
                  ....*....|....*
gi 2169866323  73 AKMTYIFWLDADDVL 87
Cdd:COG1215   110 ARGDIVVFLDADTVL 124
PRK13915 PRK13915
putative glucosyl-3-phosphoglycerate synthase; Provisional
 3-51 3.30e-08

putative glucosyl-3-phosphoglycerate synthase; Provisional


Pssm-ID: 237556 [Multi-domain]  Cd Length: 306  Bit Score: 54.15  E-value: 3.30e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTV-----YDLVDEIIIVDTGSIDRTKEIALTYGA 51
Cdd:PRK13915   32 TVSVVLPALNEEETVGKVVDSIrpllmEPLVDELIVIDSGSTDATAERAAAAGA 85
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 10-84 1.02e-07

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 51.42  E-value: 1.02e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  10 VRDEEESLGRCLSTVYDL-----VDEIIIVDTGSIDRTKEIALTYGAQlfdFTWVDDFSAARNF--------AFAQAKMT 76
Cdd:cd04179     5 AYNEEENIPELVERLLAVleegyDYEIIVVDDGSTDGTAEIARELAAR---VPRVRVIRLSRNFgkgaavraGFKAARGD 81


                  ....*...
gi 2169866323  77 YIFWLDAD 84
Cdd:cd04179    82 IVVTMDAD 89
Succinoglycan_BP_ExoA cd02525
ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA ...
 3-84 1.23e-07

ExoA is involved in the biosynthesis of succinoglycan; Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.


Pssm-ID: 133016 [Multi-domain]  Cd Length: 249  Bit Score: 52.23  E-value: 1.23e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   3 TVSLCMIVRDEEESLGRCLSTV----YDLVD-EIIIVDTGSIDRTKEIALTYGAQLFDFTWVDD----FSAARNFAFAQA 73
Cdd:cd02525     1 FVSIIIPVRNEEKYIEELLESLlnqsYPKDLiEIIVVDGGSTDGTREIVQEYAAKDPRIRLIDNpkriQSAGLNIGIRNS 80

                          90
                  ....*....|.
gi 2169866323  74 KMTYIFWLDAD 84
Cdd:cd02525    81 RGDIIIRVDAH 91
CESA_like cd06423
CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily ...
 11-96 2.69e-07

CESA_like is the cellulose synthase superfamily; The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.


Pssm-ID: 133045 [Multi-domain]  Cd Length: 180  Bit Score: 50.30  E-value: 2.69e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  11 RDEEESLGRCLSTVYDL---VDEIIIVDTGSIDRTKEIALTYGAQLFD-FTWVDDF-----SAARNFAFAQAKMTYIFWL 81
Cdd:cd06423     6 YNEEAVIERTIESLLALdypKLEVIVVDDGSTDDTLEILEELAALYIRrVLVVRDKenggkAGALNAGLRHAKGDIVVVL 85

                          90       100
                  ....*....|....*....|
gi 2169866323  82 DAD-----DVLLEADRERFA 96
Cdd:cd06423    86 DADtilepDALKRLVVPFFA 105
PRK10073 PRK10073
putative glycosyl transferase; Provisional
 30-105 5.87e-07

putative glycosyl transferase; Provisional


Pssm-ID: 182223 [Multi-domain]  Cd Length: 328  Bit Score: 50.81  E-value: 5.87e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  30 EIIIVDTGSIDRTKEIALTYGAQLFDFTWVDD----FSAARNFAFAQAKMTYIFWLDADDVLLEADRERFAALKASDNFD 105
Cdd:PRK10073   37 EIIIVNDGSTDNSVEIAKHYAENYPHVRLLHQanagVSVARNTGLAVATGKYVAFPDADDVVYPTMYETLMTMALEDDLD 116
GT_2_like_a cd02522
GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; ...
 10-103 6.08e-06

GT_2_like_a represents a glycosyltransferase family-2 subfamily with unknown function; Glycosyltransferase family 2 (GT-2) subfamily of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133013 [Multi-domain]  Cd Length: 221  Bit Score: 46.79  E-value: 6.08e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  10 VRDEEESLGRCLSTVYDLVD---EIIIVDTGSIDRTKEIALTYGAQLfdftwvddFSAAR------NFAFAQAKMTYIFW 80
Cdd:cd02522     7 TLNEAENLPRLLASLRRLNPlplEIIVVDGGSTDGTVAIARSAGVVV--------ISSPKgrarqmNAGAAAARGDWLLF 78

                          90       100
                  ....*....|....*....|...
gi 2169866323  81 LDAdDVLLEADRERFAALKASDN 103
Cdd:cd02522    79 LHA-DTRLPPDWDAAIIETLRAD 100
GT_2_WfgS_like cd06433
WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella ...
 14-145 1.23e-05

WfgS and WfeV are involved in O-antigen biosynthesis; Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133055 [Multi-domain]  Cd Length: 202  Bit Score: 45.61  E-value: 1.23e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  14 EESLGRCLSTV----YDLVdEIIIVDTGSIDRTKEIALTYGAQLFDFTWVDDF--SAARNFAFAQAKMTYIFWLDADDVL 87
Cdd:cd06433    10 AETLEETIDSVlsqtYPNI-EYIVIDGGSTDGTVDIIKKYEDKITYWISEPDKgiYDAMNKGIALATGDIIGFLNSDDTL 88

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2169866323  88 LEADrerfAALKASDNFDYDSVSMPY-HLTVDERGKPVHYLRR----NRLVKRKCNFVWHGAV 145
Cdd:cd06433    89 LPGA----LLAVVAAFAEHPEVDVVYgDVLLVDENGRVIGRRRpppfLDKFLLYGMPICHQAT 147
DPG_synthase cd04188
DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate ...
 13-84 2.36e-05

DPG_synthase is involved in protein N-linked glycosylation; UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate.


Pssm-ID: 133031 [Multi-domain]  Cd Length: 211  Bit Score: 44.87  E-value: 2.36e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  13 EEESLGRCLSTVYDLVD-------EIIIVDTGSIDRTKEIALTYGAQLFDFTWVDDFS------AARNFAFAQAKMTYIF 79
Cdd:cd04188     8 EEKRLPPTLEEAVEYLEerpsfsyEIIVVDDGSKDGTAEVARKLARKNPALIRVLTLPknrgkgGAVRAGMLAARGDYIL 87


                  ....*
gi 2169866323  80 WLDAD 84
Cdd:cd04188    88 FADAD 92
Spy COG3914
Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational ...
 194-288 2.92e-05

Predicted O-linked N-acetylglucosamine transferase, SPINDLY family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 443119 [Multi-domain]  Cd Length: 658  Bit Score: 45.75  E-value: 2.92e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323 194 FYFANELFDHARYEEAAEQYQLFLDGGLGWIEdqiwACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICCRMGAFFV 273
Cdd:COG3914   116 FNLGNLLLALGRLEEALAALRRALALNPDFAE----AYLNLGEALRRLGRLEEAIAALRRALELDPDNAEALNNLGNALQ 191

                          90
                  ....*....|....*
gi 2169866323 274 EKQDFPKAIYWYRQA 288
Cdd:COG3914   192 DLGRLEEAIAAYRRA 206
TPR COG0457
Tetratricopeptide (TPR) repeat [General function prediction only];
194-350 1.00e-04

Tetratricopeptide (TPR) repeat [General function prediction only];


Pssm-ID: 440225 [Multi-domain]  Cd Length: 245  Bit Score: 43.46  E-value: 1.00e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323 194 FYFANELFDHARYEEAAEQYQ--LFLDgglgwiEDQIWACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICCRMGAF 271
Cdd:COG0457    12 NNLGLAYRRLGRYEEAIEDYEkaLELD------PDDAEALYNLGLAYLRLGRYEEALADYEQALELDPDDAEALNNLGLA 85

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2169866323 272 FVEKQDFPKAIYWYRQATLLeRPQDSMgkvdesawtwlPHIQLCYCYDRIGDQRKAKIHHDMAKLFYPTHPSVAYNEAY 350
Cdd:COG0457    86 LQALGRYEEALEDYDKALEL-DPDDAE-----------ALYNLGLALLELGRYDEAIEAYERALELDPDDADALYNLGI 152
DPM1_like_bac cd04187
Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes ...
 10-84 3.78e-04

Bacterial DPM1_like enzymes are related to eukaryotic DPM1; A family of bacterial enzymes related to eukaryotic DPM1; Although the mechanism of eukaryotic enzyme is well studied, the mechanism of the bacterial enzymes is not well understood. The eukaryotic DPM1 is the catalytic subunit of eukaryotic Dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. The enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133030 [Multi-domain]  Cd Length: 181  Bit Score: 40.92  E-value: 3.78e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  10 VRDEEESLGRCLSTVYDLVD------EIIIVDTGSIDRTKEIALTYGAQ-----LFDFtwvddfsaARNF--------AF 70
Cdd:cd04187     5 VYNEEENLPELYERLKAVLEslgydyEIIFVDDGSTDRTLEILRELAARdprvkVIRL--------SRNFgqqaallaGL 76

                          90
                  ....*....|....
gi 2169866323  71 AQAKMTYIFWLDAD 84
Cdd:cd04187    77 DHARGDAVITMDAD 90
GT_2_like_c cd04186
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 8-91 6.51e-04

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133029 [Multi-domain]  Cd Length: 166  Bit Score: 39.85  E-value: 6.51e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323   8 MIVRDEEESLGRCLSTVYDLVD---EIIIVDTGSIDRTKEIALTYGAQLFDFTWVDD--FSAARNFAFAQAKMTYIFWLD 82
Cdd:cd04186     3 IVNYNSLEYLKACLDSLLAQTYpdfEVIVVDNASTDGSVELLRELFPEVRLIRNGENlgFGAGNNQGIREAKGDYVLLLN 82


                  ....*....
gi 2169866323  83 aDDVLLEAD 91
Cdd:cd04186    83 -PDTVVEPG 90
LapB COG2956
Lipopolysaccharide biosynthesis regulator YciM/LapB, contains six TPR domains and a C-terminal ...
 193-288 6.74e-04

Lipopolysaccharide biosynthesis regulator YciM/LapB, contains six TPR domains and a C-terminal metal-binding domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442196 [Multi-domain]  Cd Length: 275  Bit Score: 40.87  E-value: 6.74e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323 193 QFYFANELFDHARYEEAAEQYQLFLDGGlgwiEDQIWACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICCRMGAFF 272
Cdd:COG2956    79 LLELAQDYLKAGLLDRAEELLEKLLELD----PDDAEALRLLAEIYEQEGDWEKAIEVLERLLKLGPENAHAYCELAELY 154

                          90
                  ....*....|....*.
gi 2169866323 273 VEKQDFPKAIYWYRQA 288
Cdd:COG2956   155 LEQGDYDEAIEALEKA 170
GT_2_like_e cd04192
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 10-84 1.13e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133035 [Multi-domain]  Cd Length: 229  Bit Score: 39.96  E-value: 1.13e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  10 VRDEEESLGRCLSTV------YDLVdEIIIVDTGSIDRTKEIALTYGA----QLFDFTWVDDFSAARNFAFAQ----AKM 75
Cdd:cd04192     5 ARNEAENLPRLLQSLsaldypKEKF-EVILVDDHSTDGTVQILEFAAAkpnfQLKILNNSRVSISGKKNALTTaikaAKG 83


                  ....*....
gi 2169866323  76 TYIFWLDAD 84
Cdd:cd04192    84 DWIVTTDAD 92
PRK14720 PRK14720
transcription elongation factor GreA;
226-288 2.02e-03

transcription elongation factor GreA;


Pssm-ID: 184804 [Multi-domain]  Cd Length: 906  Bit Score: 40.14  E-value: 2.02e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2169866323 226 DQIWACQKQSECYAHLKQPEKQVQSLTRTLAIDLPRAEICcRMGAFFVEKQDFPKAIYWYRQA 288
Cdd:PRK14720  114 ENKLALRTLAEAYAKLNENKKLKGVWERLVKADRDNPEIV-KKLATSYEEEDKEKAITYLKKA 175
GT2_Chondriotin_Pol_N cd06420
N-terminal domain of Chondroitin polymerase functions as a GalNAc transferase; Chondroitin ...
 15-84 3.26e-03

N-terminal domain of Chondroitin polymerase functions as a GalNAc transferase; Chondroitin polymerase is a two domain, bi-functional protein. The N-terminal domain functions as a GalNAc transferase. The bacterial chondroitin polymerase catalyzes elongation of the chondroitin chain by alternatively transferring the GlcUA and GalNAc moiety from UDP-GlcUA and UDP-GalNAc to the non-reducing ends of the chondroitin chain. The enzyme consists of N-terminal and C-terminal domains in which the two active sites catalyze the addition of GalNAc and GlcUA, respectively. Chondroitin chains range from 40 to over 100 repeating units of the disaccharide. Sulfated chondroitins are involved in the regulation of various biological functions such as central nervous system development, wound repair, infection, growth factor signaling, and morphogenesis, in addition to its conventional structural roles. In Caenorhabditis elegans, chondroitin is an essential factor for the worm to undergo cytokinesis and cell division. Chondroitin is synthesized as proteoglycans, sulfated and secreted to the cell surface or extracellular matrix.


Pssm-ID: 133042 [Multi-domain]  Cd Length: 182  Bit Score: 37.94  E-value: 3.26e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  15 ESLGRCLSTVY---DLVDEIIIVDTGSIDRTKEIALTYGAQlFDFT----WVDD--FSAA--RNFAFAQAKMTYIFWLDA 83
Cdd:cd06420    10 EALELVLKSVLnqsILPFEVIIADDGSTEETKELIEEFKSQ-FPIPikhvWQEDegFRKAkiRNKAIAAAKGDYLIFIDG 88


                  .
gi 2169866323  84 D 84
Cdd:cd06420    89 D 89
GT_2_like_d cd04196
Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse ...
 29-126 4.38e-03

Subfamily of Glycosyltransferase Family GT2 of unknown function; GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.


Pssm-ID: 133039 [Multi-domain]  Cd Length: 214  Bit Score: 38.00  E-value: 4.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2169866323  29 DEIIIVDTGSIDRTKEIALTYgAQLFDFTWVDDFS-----AARNF--AFAQAKMTYIFWLDADDVLLEADRERFaaLKAs 101
Cdd:cd04196    28 DELIISDDGSTDGTVEIIKEY-IDKDPFIIILIRNgknlgVARNFesLLQAADGDYVFFCDQDDIWLPDKLERL--LKA- 103

                          90       100
                  ....*....|....*....|....*..
gi 2169866323 102 dNFDYDSVSMPYH--LTVDERGKPVHY 126
Cdd:cd04196   104 -FLKDDKPLLVYSdlELVDENGNPIGE 129
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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