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Conserved domains on  [gi|2721362262|ref|WP_342168318|]
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PglL family O-oligosaccharyltransferase [Serratia nevei]

Protein Classification

PglL family O-oligosaccharyltransferase( domain architecture ID 11462228)

PglL family O-oligosaccharyltransferase catalyzes the O-glycosylation of protein targets, similar to Acinetobacter baylyi general O-oligosaccharyltransferase and Neisseria meningitidis PglL that is responsible for O-glycosylation of type IV pilins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RfaL COG3307
O-antigen ligase [Cell wall/membrane/envelope biogenesis];
273-404 1.49e-17

O-antigen ligase [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 442536 [Multi-domain]  Cd Length: 239  Bit Score: 82.05  E-value: 1.49e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 273 SGLIAREGSNYSRLTMLQDTLSMILAKPLLGWGYGGFEYSFAHFRLQAMPWRevlevAGHPHNEILLWWVEGGLPALAGI 352
Cdd:COG3307   106 LEILGKDSSLGGRLEIWKAALEMIKEHPLLGVGYGNFWYAYPAYQPAGYIWN-----AGHAHNEYLEILAETGILGLLLL 180

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2721362262 353 VIVLIAgalLLKRAWQRERKqpAGARVGLFLVLLPMLVHTQLEYPFYLSAPH 404
Cdd:COG3307   181 LLLLLV---LLRRALRRLRR--SRLALALLALLLALLVHGLTESPLFSPASA 227
Wzy_C_2 super family cl20428
Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of ...
 381-553 5.70e-08

Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of 12 TMs, found in Gram-positive bacteria such as Streptococcus pnuemoniae. It forms part of the EPS or exopolysaccharide system. This family is the 6xTMs at the C-terminal end of the molecule. Wzy functions in polymerizing the oligosaccharide repeat subunits to form high-molecular-weight capsular polysaccharides. A contiguous emebrane-bound flippase, Wzx, pfam01943, transports the repeat units to the external surface of the membrane. These polysaccharides form the capsule and their differing compositions contribute to the multidudinous pneumococcal capsular serotypes, all being structurally and antigenically different.


The actual alignment was detected with superfamily member pfam11846:

Pssm-ID: 432124  Cd Length: 186  Bit Score: 53.13  E-value: 5.70e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 381 LFLVLLPMLVHTQLEYPFYLSAPHWLAFLLLLALLDGQ-TGEPRPLPFAKALSLPVAMAAVGVLVMAVFAWQGRMaLTQS 459
Cdd:pfam11846   2 LLALLAPILLHSLLEYPLWYSPFLLPAFLVLGLLDPNYrWRRLRLRPKGVLRVVALVLPILGVAYLLSILHDYQR-AEQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 460 ERTMLATIDSIEQmpPPAAWIYRERKTFDEQSYALLVYNQTRDDALLTGYRQWANAYLQRRIDANVYATLIMILRYQGAQ 539
Cdd:pfam11846  81 YYTSRPNEQALRD--VTNPLVWQDRFLFRAYADFLRLGLLPPTPENLADKLALARKLIHLSPEPAVYERLALSLALLGDP 158

                         170
                  ....*....|....
gi 2721362262 540 AEADARLREAAFLF 553
Cdd:pfam11846 159 DEALQTLARARALF 172
 
Name Accession Description Interval E-value
RfaL COG3307
O-antigen ligase [Cell wall/membrane/envelope biogenesis];
273-404 1.49e-17

O-antigen ligase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442536 [Multi-domain]  Cd Length: 239  Bit Score: 82.05  E-value: 1.49e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 273 SGLIAREGSNYSRLTMLQDTLSMILAKPLLGWGYGGFEYSFAHFRLQAMPWRevlevAGHPHNEILLWWVEGGLPALAGI 352
Cdd:COG3307   106 LEILGKDSSLGGRLEIWKAALEMIKEHPLLGVGYGNFWYAYPAYQPAGYIWN-----AGHAHNEYLEILAETGILGLLLL 180

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2721362262 353 VIVLIAgalLLKRAWQRERKqpAGARVGLFLVLLPMLVHTQLEYPFYLSAPH 404
Cdd:COG3307   181 LLLLLV---LLRRALRRLRR--SRLALALLALLLALLVHGLTESPLFSPASA 227
Wzy_C_2 pfam11846
Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of ...
 381-553 5.70e-08

Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of 12 TMs, found in Gram-positive bacteria such as Streptococcus pnuemoniae. It forms part of the EPS or exopolysaccharide system. This family is the 6xTMs at the C-terminal end of the molecule. Wzy functions in polymerizing the oligosaccharide repeat subunits to form high-molecular-weight capsular polysaccharides. A contiguous emebrane-bound flippase, Wzx, pfam01943, transports the repeat units to the external surface of the membrane. These polysaccharides form the capsule and their differing compositions contribute to the multidudinous pneumococcal capsular serotypes, all being structurally and antigenically different.


Pssm-ID: 432124  Cd Length: 186  Bit Score: 53.13  E-value: 5.70e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 381 LFLVLLPMLVHTQLEYPFYLSAPHWLAFLLLLALLDGQ-TGEPRPLPFAKALSLPVAMAAVGVLVMAVFAWQGRMaLTQS 459
Cdd:pfam11846   2 LLALLAPILLHSLLEYPLWYSPFLLPAFLVLGLLDPNYrWRRLRLRPKGVLRVVALVLPILGVAYLLSILHDYQR-AEQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 460 ERTMLATIDSIEQmpPPAAWIYRERKTFDEQSYALLVYNQTRDDALLTGYRQWANAYLQRRIDANVYATLIMILRYQGAQ 539
Cdd:pfam11846  81 YYTSRPNEQALRD--VTNPLVWQDRFLFRAYADFLRLGLLPPTPENLADKLALARKLIHLSPEPAVYERLALSLALLGDP 158

                         170
                  ....*....|....
gi 2721362262 540 AEADARLREAAFLF 553
Cdd:pfam11846 159 DEALQTLARARALF 172
Wzy_C pfam04932
O-Antigen ligase; This group of bacterial proteins is involved in the synthesis of O-antigen, ...
 277-353 8.08e-08

O-Antigen ligase; This group of bacterial proteins is involved in the synthesis of O-antigen, a lipopolysaccharide found in the outer membrane in gram-negative bacteria. This family includes O-antigen ligases such as E. coli RfaL.


Pssm-ID: 461489  Cd Length: 148  Bit Score: 51.63  E-value: 8.08e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2721362262 277 AREGSNYSRLTMLQDTLSMILAKPLLGWGYGGFEYSFAHFRLQAMPwreVLEVAGHPHNEILLWWVEGGLPALAGIV 353
Cdd:pfam04932  75 AKDGSSSGRLEIWKSALDLIKDHPLLGVGAGTGLAYAAYLLYAQLL---GSGGYTHAHNLYLEILAELGIPGLLLFL 148
 
Name Accession Description Interval E-value
RfaL COG3307
O-antigen ligase [Cell wall/membrane/envelope biogenesis];
273-404 1.49e-17

O-antigen ligase [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 442536 [Multi-domain]  Cd Length: 239  Bit Score: 82.05  E-value: 1.49e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 273 SGLIAREGSNYSRLTMLQDTLSMILAKPLLGWGYGGFEYSFAHFRLQAMPWRevlevAGHPHNEILLWWVEGGLPALAGI 352
Cdd:COG3307   106 LEILGKDSSLGGRLEIWKAALEMIKEHPLLGVGYGNFWYAYPAYQPAGYIWN-----AGHAHNEYLEILAETGILGLLLL 180

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2721362262 353 VIVLIAgalLLKRAWQRERKqpAGARVGLFLVLLPMLVHTQLEYPFYLSAPH 404
Cdd:COG3307   181 LLLLLV---LLRRALRRLRR--SRLALALLALLLALLVHGLTESPLFSPASA 227
Wzy_C_2 pfam11846
Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of ...
 381-553 5.70e-08

Virulence factor membrane-bound polymerase, C-terminal; Wzy is a membrane-bound polymerase of 12 TMs, found in Gram-positive bacteria such as Streptococcus pnuemoniae. It forms part of the EPS or exopolysaccharide system. This family is the 6xTMs at the C-terminal end of the molecule. Wzy functions in polymerizing the oligosaccharide repeat subunits to form high-molecular-weight capsular polysaccharides. A contiguous emebrane-bound flippase, Wzx, pfam01943, transports the repeat units to the external surface of the membrane. These polysaccharides form the capsule and their differing compositions contribute to the multidudinous pneumococcal capsular serotypes, all being structurally and antigenically different.


Pssm-ID: 432124  Cd Length: 186  Bit Score: 53.13  E-value: 5.70e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 381 LFLVLLPMLVHTQLEYPFYLSAPHWLAFLLLLALLDGQ-TGEPRPLPFAKALSLPVAMAAVGVLVMAVFAWQGRMaLTQS 459
Cdd:pfam11846   2 LLALLAPILLHSLLEYPLWYSPFLLPAFLVLGLLDPNYrWRRLRLRPKGVLRVVALVLPILGVAYLLSILHDYQR-AEQL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2721362262 460 ERTMLATIDSIEQmpPPAAWIYRERKTFDEQSYALLVYNQTRDDALLTGYRQWANAYLQRRIDANVYATLIMILRYQGAQ 539
Cdd:pfam11846  81 YYTSRPNEQALRD--VTNPLVWQDRFLFRAYADFLRLGLLPPTPENLADKLALARKLIHLSPEPAVYERLALSLALLGDP 158

                         170
                  ....*....|....
gi 2721362262 540 AEADARLREAAFLF 553
Cdd:pfam11846 159 DEALQTLARARALF 172
Wzy_C pfam04932
O-Antigen ligase; This group of bacterial proteins is involved in the synthesis of O-antigen, ...
 277-353 8.08e-08

O-Antigen ligase; This group of bacterial proteins is involved in the synthesis of O-antigen, a lipopolysaccharide found in the outer membrane in gram-negative bacteria. This family includes O-antigen ligases such as E. coli RfaL.


Pssm-ID: 461489  Cd Length: 148  Bit Score: 51.63  E-value: 8.08e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2721362262 277 AREGSNYSRLTMLQDTLSMILAKPLLGWGYGGFEYSFAHFRLQAMPwreVLEVAGHPHNEILLWWVEGGLPALAGIV 353
Cdd:pfam04932  75 AKDGSSSGRLEIWKSALDLIKDHPLLGVGAGTGLAYAAYLLYAQLL---GSGGYTHAHNLYLEILAELGIPGLLLFL 148
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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