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Conserved domains on  [gi|2746980425|ref|WP_350359749|]
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glycosyltransferase [Xanthomonas citri]

Protein Classification

glycosyltransferase family 2 protein( domain architecture ID 12936640)

glycosyltransferase family 2 protein catalyzes the transfer of saccharide moieties from a donor to an acceptor to form glycosidic bonds

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 2-108 4.83e-24

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


:

Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 94.95  E-value: 4.83e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGrMPKMRIIGNAALSFLTKLSSGyWDIFDPTNGYTAV 81
Cdd:cd04179    81 DIVVTMDADLQHPPEDIPKLLEKLLEGGADVVIGSRFVRGGGAG-MPLLRRLGSRLFNFLIRLLLG-VRISDTQSGFRLF 158

                          90       100
                  ....*....|....*....|....*..
gi 2746980425  82 HASVVARMPLDKISRRYFFESDMLFRL 108
Cdd:cd04179   159 RREVLEALLSLLESNGFEFGLELLVGA 185
glyco_TIGR04182 super family cl37399
glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases ...
 2-234 2.36e-10

glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases restricted to the archaea. All but two members are from species with the PGF-CTERM/archaeosortase A system, a proposed maturation system for exported, glycosylated proteins as are found often in S-layers.


The actual alignment was detected with superfamily member TIGR04182:

Pssm-ID: 275035  Cd Length: 293  Bit Score: 59.23  E-value: 2.36e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQiGRMPKMRIIGNAALSFLTKLSSGYwDIFDPTNGYTAV 81
Cdd:TIGR04182  74 PYVLMLDGDGTYLPADADALLEPLLSGRADHVIGNRFADMEP-GAFTRLNLVGNRLINRLFRIIHGV-DLRDILSGYRAF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425  82 HASVVARMPLDKISrrYFFESDMLFR--LNIIRAVVVDVPMDARYGDEVSNLS-VR---KIvldfaIRHVRNFGKR---I 152
Cdd:TIGR04182 152 TRESIRRMELTETG--FEIETEIAVEcvKKNLRVEVVPITYRARPDGSDTKLNpLRdgfKI-----GLTIYRLAKTnnpL 224

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425 153 FynYFlrdlslasleLVAGVLFLFSGATVGVFF---WLQSaqtgyFSSAGSVMLAALQVIVGIQLIL-GFLAYDIAAVPA 228
Cdd:TIGR04182 225 F--YF----------GIIGAIFGLAGFLLGIYVvveWLKG-----ISHEPLAILTALLIIFGVQLFMfGVLSDMILTLHR 287


                  ....*.
gi 2746980425 229 RTLHLL 234
Cdd:TIGR04182 288 EQMREI 293
 
Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 2-108 4.83e-24

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 94.95  E-value: 4.83e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGrMPKMRIIGNAALSFLTKLSSGyWDIFDPTNGYTAV 81
Cdd:cd04179    81 DIVVTMDADLQHPPEDIPKLLEKLLEGGADVVIGSRFVRGGGAG-MPLLRRLGSRLFNFLIRLLLG-VRISDTQSGFRLF 158

                          90       100
                  ....*....|....*....|....*..
gi 2746980425  82 HASVVARMPLDKISRRYFFESDMLFRL 108
Cdd:cd04179   159 RREVLEALLSLLESNGFEFGLELLVGA 185
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 2-137 8.76e-12

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 62.41  E-value: 8.76e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIgrmpKMRIIGNAALSFLTKLSsgywDIFDPTNGYTAV 81
Cdd:COG0463    84 DYIAFLDADDQLDPEKLEELVAALEEGPADLVYGSRLIREGES----DLRRLGSRLFNLVRLLT----NLPDSTSGFRLF 155

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2746980425  82 HASVVARMPLDKisrRYFFESDMLfRLNIIRAVVVDVPMdaRYGDEVSNLSVRKIV 137
Cdd:COG0463   156 RREVLEELGFDE---GFLEDTELL-RALRHGFRIAEVPV--RYRAGESKLNLRDLL 205
glyco_TIGR04182 TIGR04182
glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases ...
 2-234 2.36e-10

glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases restricted to the archaea. All but two members are from species with the PGF-CTERM/archaeosortase A system, a proposed maturation system for exported, glycosylated proteins as are found often in S-layers.


Pssm-ID: 275035  Cd Length: 293  Bit Score: 59.23  E-value: 2.36e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQiGRMPKMRIIGNAALSFLTKLSSGYwDIFDPTNGYTAV 81
Cdd:TIGR04182  74 PYVLMLDGDGTYLPADADALLEPLLSGRADHVIGNRFADMEP-GAFTRLNLVGNRLINRLFRIIHGV-DLRDILSGYRAF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425  82 HASVVARMPLDKISrrYFFESDMLFR--LNIIRAVVVDVPMDARYGDEVSNLS-VR---KIvldfaIRHVRNFGKR---I 152
Cdd:TIGR04182 152 TRESIRRMELTETG--FEIETEIAVEcvKKNLRVEVVPITYRARPDGSDTKLNpLRdgfKI-----GLTIYRLAKTnnpL 224

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425 153 FynYFlrdlslasleLVAGVLFLFSGATVGVFF---WLQSaqtgyFSSAGSVMLAALQVIVGIQLIL-GFLAYDIAAVPA 228
Cdd:TIGR04182 225 F--YF----------GIIGAIFGLAGFLLGIYVvveWLKG-----ISHEPLAILTALLIIFGVQLFMfGVLSDMILTLHR 287


                  ....*.
gi 2746980425 229 RTLHLL 234
Cdd:TIGR04182 288 EQMREI 293
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 2-88 7.57e-06

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 44.69  E-value: 7.57e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGRMPKMRIIGNAALSFLTKLSSGYWDIFDPTNGYTAV 81
Cdd:pfam00535  80 DYIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGLNLPFLIGGFALY 159


                  ....*..
gi 2746980425  82 HASVVAR 88
Cdd:pfam00535 160 RREALEE 166
 
Name Accession Description Interval E-value
DPM_DPG-synthase_like cd04179
DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the ...
 2-108 4.83e-24

DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133022 [Multi-domain]  Cd Length: 185  Bit Score: 94.95  E-value: 4.83e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGrMPKMRIIGNAALSFLTKLSSGyWDIFDPTNGYTAV 81
Cdd:cd04179    81 DIVVTMDADLQHPPEDIPKLLEKLLEGGADVVIGSRFVRGGGAG-MPLLRRLGSRLFNFLIRLLLG-VRISDTQSGFRLF 158

                          90       100
                  ....*....|....*....|....*..
gi 2746980425  82 HASVVARMPLDKISRRYFFESDMLFRL 108
Cdd:cd04179   159 RREVLEALLSLLESNGFEFGLELLVGA 185
DPM1_like cd06442
DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to ...
 2-137 5.54e-13

DPM1_like represents putative enzymes similar to eukaryotic DPM1; Proteins similar to eukaryotic DPM1, including enzymes from bacteria and archaea; DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. This protein family belongs to Glycosyltransferase 2 superfamily.


Pssm-ID: 133062 [Multi-domain]  Cd Length: 224  Bit Score: 66.02  E-value: 5.54e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGRMPKMRIIGNAALSFLTKLSSGYwDIFDPTNGYTAV 81
Cdd:cd06442    80 DVIVVMDADLSHPPEYIPELLEAQLEGGADLVIGSRYVEGGGVEGWGLKRKLISRGANLLARLLLGR-KVSDPTSGFRAY 158

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2746980425  82 HASVVARMPLDKISRRYFFESDMLFRLNIIRAVVVDVP---MDARYGdeVSNLSVRKIV 137
Cdd:cd06442   159 RREVLEKLIDSLVSKGYKFQLELLVRARRLGYRIVEVPitfVDREHG--ESKLGGKEIV 215
WcaA COG0463
Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis]; ...
 2-137 8.76e-12

Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440231 [Multi-domain]  Cd Length: 208  Bit Score: 62.41  E-value: 8.76e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIgrmpKMRIIGNAALSFLTKLSsgywDIFDPTNGYTAV 81
Cdd:COG0463    84 DYIAFLDADDQLDPEKLEELVAALEEGPADLVYGSRLIREGES----DLRRLGSRLFNLVRLLT----NLPDSTSGFRLF 155

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*.
gi 2746980425  82 HASVVARMPLDKisrRYFFESDMLfRLNIIRAVVVDVPMdaRYGDEVSNLSVRKIV 137
Cdd:COG0463   156 RREVLEELGFDE---GFLEDTELL-RALRHGFRIAEVPV--RYRAGESKLNLRDLL 205
glyco_TIGR04182 TIGR04182
glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases ...
 2-234 2.36e-10

glycosyltransferase, TIGR04182 family; Members of this family are glycosyltransferases restricted to the archaea. All but two members are from species with the PGF-CTERM/archaeosortase A system, a proposed maturation system for exported, glycosylated proteins as are found often in S-layers.


Pssm-ID: 275035  Cd Length: 293  Bit Score: 59.23  E-value: 2.36e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQiGRMPKMRIIGNAALSFLTKLSSGYwDIFDPTNGYTAV 81
Cdd:TIGR04182  74 PYVLMLDGDGTYLPADADALLEPLLSGRADHVIGNRFADMEP-GAFTRLNLVGNRLINRLFRIIHGV-DLRDILSGYRAF 151

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425  82 HASVVARMPLDKISrrYFFESDMLFR--LNIIRAVVVDVPMDARYGDEVSNLS-VR---KIvldfaIRHVRNFGKR---I 152
Cdd:TIGR04182 152 TRESIRRMELTETG--FEIETEIAVEcvKKNLRVEVVPITYRARPDGSDTKLNpLRdgfKI-----GLTIYRLAKTnnpL 224

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425 153 FynYFlrdlslasleLVAGVLFLFSGATVGVFF---WLQSaqtgyFSSAGSVMLAALQVIVGIQLIL-GFLAYDIAAVPA 228
Cdd:TIGR04182 225 F--YF----------GIIGAIFGLAGFLLGIYVvveWLKG-----ISHEPLAILTALLIIFGVQLFMfGVLSDMILTLHR 287


                  ....*.
gi 2746980425 229 RTLHLL 234
Cdd:TIGR04182 288 EQMREI 293
Glycos_transf_2 pfam00535
Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, ...
 2-88 7.57e-06

Glycosyl transferase family 2; Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.


Pssm-ID: 425738 [Multi-domain]  Cd Length: 166  Bit Score: 44.69  E-value: 7.57e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2746980425   2 EIIVKMDGDGQMDPDALPELIGPIVRGEADYTKGNRFYDLAQIGRMPKMRIIGNAALSFLTKLSSGYWDIFDPTNGYTAV 81
Cdd:pfam00535  80 DYIAFLDADDEVPPDWLEKLVEALEEDGADVVVGSRYVIFGETGEYRRASRITLSRLPFFLGLRLLGLNLPFLIGGFALY 159


                  ....*..
gi 2746980425  82 HASVVAR 88
Cdd:pfam00535 160 RREALEE 166
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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